고려 엉겅퀴(Cirsium setidens Nakai) 잎 조직을 이용한 callus 배양 및 항산화 활성 검증

박정훈,심예지,박기임,이인순,문혜연 대구대학교 특수교육재활과학연구소 2010 産業技術硏究 Vol.21 No.1

We described the effects of antioxidant activity on the extracts of tissue and callus formation by Cirsium setidens Nakai. The callus culture were induced from leaf tissue of Cirsium setidens in both MS and B5 medium with IAA or/and kinetin (0∼5.0 ㎎/ℓ). As medium for callus culture induced from Cirsium setidens, B5 medium showed better condition than MS media. MS medium brought to decrease callus differentiation after formation, on the other hand B5 medium induced to accelerate callus differentiation. Among phytohormones is required 0.5 ㎎/ℓ of IAA and between 2.0 ㎎/ℓ and 1.0 ㎎/ℓ of Kinetin for formation of callus. In order to extract functional material from natural leaf and callus tissue of Cirsium setidens used distilled water and 75% ethanol as solvent and those extract used to determine antioxidant activity as alpha-diphenyl-2-picryl-hydrozyl (DPPH) radicals scavenging effect and TBA(thiobarbituric acid) activity. In result, the effect of DPPH and TBA had high activity more natural leaf extract than callus tissue and 75% ethanol was solvent better than distilled water. The 75% ethanol extract of natural leaf showed an excellent antioxidant activity because that particularly had highest concentration of vitamin C and total phenol among those extracts

벼 생육 및 수집에 대한 대체농자재의 영향

문영훈,최정식,엄미정,한성수 한국환경농학회 2003 한국환경농학회지 Vol.22 No.4

본 시험은 환경농업기술을 직접 농가에 파급시킬 목적으로 3년간('96~'98)수행하였던 연구결과를 토대로 1999년에 환경농업 시범마을인 남원시 대강면 방산리의 일반농가포장에서 수행한 결과이다. 계분 발효퇴비와 깻묵을 시용한 환경농업과 절충형농업 시험구의 벼 생육은 화학비료를 시용한 일반관행농업 시험구에 비하여 저조하였으며, 왕우렁이(Ampullarius insularus)를 이용한 잡초방제 효과는 제초제 2회(초·중기)살포효과보다 우수하였다. 목초액의 병해충 방제효과는 저조하였고 또한 환경농업과 절충형농업 시험구의 정조수량은 일반관행농업 시험구에 비하여 3~5% 감소 되였다. 수질은 환경농업과 절충형농업 시험구가 일반관행농업 시험구에 비하여 COD, NO₃-N, SO₄^(2-), Cl^(-)이 낮았으며, 물벼룩의 밀도는 높았다. The aim of this study was to apply the new agricultural technique developed for three years(96-98) by demonstration at an model farm of environmental agriculture located in Bangsan-ri, Dagang-myun, Namwon-city. Rice growth of environmental farming plot, which supplied with oil cake and compost fermented with chicken manure was reduced compared to that of conventional farming plot, which supplied with chemical fertilizers. Weed control by using of Ampullarius insularus in the rice paddy field was effective compared to that of control, which treated with chemical herbicide twice. The yield of rice was decreased of 3-5% in both environmental and eclectic farming good compared to that of conventional farming plot. The water quality of environmental not and eclectic farming plot were good compared to conventional farming plot. Density of water flea was high in environmental and eclectic farming plot compared to that of the conventional farming plot.

산국 (Chrysanthemum boreale) 휘발성 추출액 및 EDTA가 미생물 및 인체 치은 섬유아세포에 미치는 영향

차정단,김태영,우원홍,전병훈,김해경,유용욱,김강주,길봉섭 원광대학교 생체재료·매식연구소 2000 원광생체재료·매식 Vol.9 No.1

Chrysanthemum boreale (C. boreale) has been used for the treatment of furuncles, carbuncles, sores, conjunctivitis, headache, vertigo and swollen throats as a folk medicine in Korea, but there is little report about experiment evidence of antimicrobial activity. To investigate the antifungal and antibacterial activity of Chrysanthemum boreale(C. boreale) volatile substance and EDTA, the growth inhibition against Aspergillus nidulans (A. nidulans), Fusarium oxysporum (F. oxysporum), Alternaria mali(Al. mali), Candida albicans (C. albicans), staphylococcus aureus (S. aureus), Streptococcus pyogenes(S. pyogenes), streptococcus mutans (S. mutans), Escherichia coli(E. coli), Helicobacter pylori (H. pylori) and Porphyromonas gingivalis (P. gingivalis) was monitored by agar dilution and broth microdilution method. The antifungal effect of C. boreale on A. nidulans, F. oxysporum, Al. mali and C. albicans was shown at the concentration of 0.04%. The antibaterial effect of C. boreale on S. aureus, S. pyogenes, S. mutans and H. pylori was shown at the concentration of 0.04%. However, the growth was inhibited at the concentration of 0.04% irrespective of the presence of EDTA 0.125% was much higher than higher than that of several concentration of volatile substance. The minimum inhibitory concentrations of volatile substance were 0.16% against S. aureus, 0.04% against S. pyogenes, 0.02% against S. mutans, 0.40% against E. coli, and 0.18% against C. albicans. The growth of human gingival fibroblasts was not affected by C. boreale volatile substances at the concentration of 0.04%. These results indicate that volatile substance from C. boreale may have biologically toxic activities to the microorganisms.

배관내에서 난류 마찰 저항감소에 관한 연구

박설현,윤정환,서동표,정대훈,오율권 朝鮮大學校 機械技術硏究所 2000 機械技術硏究 Vol.3 No.1

Polymer drag reduction can offer a attractive way to utilize ready-made facilities in crude-oil transport system without additional large expenditure. Also, drag reduction in two phase flow can be applied to phase change system such as chemical reactor, pool and pump impeller which occurs cavitation. But the research on drag reduction in two phase flow has not been investigated. Therefore, experimental investigations have been carried out to analyze the drag reduction in the single phase and two phase flow. The objectives of the proposed investigation are primarily to identify and develop high performance polymer additives for fluid transportation. And drag reduction will be evaluated in horizontal flow by measuring void fraction, pressure drop, mean liquid velocity and turbulent intensity

Coacervation-상분리법으로 만든 Ethyl Cellulose 마이크로캅셀의 Glibenclamide 방출특성 및 수용성 첨가제의 영향

김병조,장정수,이문주,김상헌,최영욱 중앙대학교 약학연구소 1996 약학 논총 Vol.10 No.-

Glibenclamide (GBC) is a well-known antidiabetic agent, which stimulates the secretion of endogenous insulin by pancreatic βcells. However, administration of GBC at a high dose can occasionally induced the fatal hypoglycemia, especially in the patients with impaired liver function. Therefore, in order to develop an oral sustained-release preparation which reduces the side effect, microcapsulation of GBC has been carried out in this experiment. Microcapsules of GBC were prepared by coacervation-phase separation method with ethylcellulose as a wall-forming material in cyclohexane, using polyisobutylene as a coacervation-inducing agent. Different amounts of hydrophilic additives. L-arginine(ARG) and polyethylene glycol 4000 (PEG), were added to the microcapsule wall, in order to alter the porosity of the wall and hence to enhance the release of the core material. The microcapsules prepared were examined for physical properties by scanning electron microscopy (SEM) and particle size analysis. The release of the poorly water-soluble GBC was found to be very slow from the microcapsules and strongly dependent on the core to wall ratio of the microcapsules, but it was accelerated considerably with increasing amount of PEG or ARG. In general, on the basis of dissolution kinetics, GBC releases from microcapsules were followed apparent first-order kinetics, while the dissolution data of GBC powder conformed to Hixon-Crowell's cube root law. Therefore, in conclusion, it might be possible to design the sustained-release formulation by the combination of both GBC powder and microcapsules of different core wall ratio with or without hydrophilic additives.

석유화학 BTX 공정 점토탑에서의 악취 및 공정 부식 방지에 관한 연구

서성규,정채훈,문정선 여수대학교 산업기술지역개발연구소 2000 産業基術硏究所 論文集 Vol.9 No.-

offensive odors affect man's olfactory sense and cause repulsiveness. In recent years, the problem of offensive odors is becoming more complicated and diversed. The emitted offensive odors from petrochemical BTX process gave rise to a serious corrosion problem of benzene tower and offensive odor problem. The main purpose in this study is to investigate process corrosion and odor generation from clay tower of petrochemical BTX process which were assumed to be the conversion of sulfur compounds over clay into hydrogen sulfide. As a result of this study, when traces of thiophenes and mercaptans are present in the clay tower, these components are broken down by the clay. The forming H□S in this step will be concentrated in the benzene tower top. In order to remove the occurred hydrogen sulfide, Fe-chelate catalyst is used. Removing efficiency is better than 99%.

CMS에서 전력제어를 위한 AGC의 성능 개선에 관한 연구

이원구,이철희,천종훈,박수봉,박종안,신용길 조선대학교 동력자원연구소 1996 動力資源硏究所誌 Vol.18 No.1

CDMA 시스템에서는 이동국이 Open-loop 전력제어 및 Close-Loop 전력제어를 할 때 비 선형적인 AGC의 특성으로 인해 수신기의 감도가 현저히 낮아진다. 본 논문에서는 AGC의 비선형적인 특성 곡선값을 S/W로 선형화 시켜 RAS RAMDATA를 생성하고 이를 RAS RAM에 넣어 선형적으로 보상된 AGC특성을 얻는 알고리즘을 제안하였다. 그리고 실험을 통하여 그 유효성을 증명한다. In CDMA system, the sensitivity of receivers is lowered significantly due to characteristics of non-linear AGC when the mobile station implements open-loop power control and closed-loop power control. In this paper, We propose a algorithm that obtains linearly conpensated AGC characteristic by inserting the RAS RAM DATA in RAS RAM, which linearizes the value of non-linear characteristic graph in AGC by S/W, and the experimental results shows the validity.

5일 보존용 채혈백에 따른 보존일자별 농축혈소판의 생화학 및 성상 분석

이동범,유태종,김태전,김승곤,신종관,양대형,고광임,박성훈 임상병리검사과학회 2002 임상병리검사과학회지 Vol.34 No.2

Biochemical changes and icon of platelets on platelet concentrates preserved for a 5day term in several plastic containers were investigated. The authors evaluated the TOTM-PVC second generation container produced by Green Cross Medical Corp. and Boin medica. Blood obtained from 40 persons was manufactured for platelet concentrates within 4 hours and each divided into 20 units of platelet concentrations. Samples were taken at 0, 3, and 5days and the platelet count, pH, pO_(2), pCO_(2), HCO_(3)-, glucose, LDH, lactate, electrolytes, and mineral element were tested The number and MPV, for platelets icon, were well preserved on two different platelet concentrates during storage. A test for biochemical function appeared to be effective data. pH was maintained above 6.8 on two different platelet concentrates during storage. The results suggest that the TOTM-PVC second generation platelets storage container produced by Green Cross Medical Corp. and Boin medica was able to preserve platelets for at least five days without lesion and was it concluded to have biochemical efficacy on patients with transfusions.

시게루 반의 재난구조 가설건축의 특성에 관한 연구

김정훈 ( Joung Hun Kim ),이일형 ( Yil Hyung Lee ) 한국기초조형학회 2016 기초조형학연구 Vol.17 No.2

본 연구는 시게루 반의 건축을 이해하는데 있어 중요한 부분인 그의 재난구조 가설건축에 대한 특성을 밝히는데 목적을 두고 있다. 우선 그의 작품세계에 근저를 이루고 있는 건축교육과 경력을 통해 받은 영향은 ① 재활용에 대한 관심 ② 실험적인 성향 ③ 지역성 추구에 대한 영향 ④ 구조를 비롯한 공학적 영향으로 정리될 수 있었다. 또한 재난구조 가설건축의 배경과 유래는 재난지역 피난민들을 위한 지속적인 자원봉사 활동을 가능하게 한 진정한 건축가로서의 역할에 따른 직업적 책임감에서 비롯되었음을 알 수 있었다. 그리고 재난구조 가설건축은 경제성·시공성·친환경성·지역성에 대한 고려를 바탕으로, 창의적인 아이디어와 지속적인 실험적 도전을 통해 종이관의 재료·구조에 대한 가공성·재활용성 및 방수성·내화성·단열성·차음성 등의 성능향상으로 그것의 효율성·가능성을 발전시켜왔다. 또한 연결 접합부도 초기의 목재에서 종이관의 사용부위가 아치·쉘·볼트골조 등으로 발전함에 따라 합성목재·직물테이프·플라스틱·금속재·성형알루미늄 등으로 다양화되어왔다. 시게루 반이 지난 30여년 동안 구축해온 재난구조 가설건축은 초기에 임시거처를 위한 종이관의 단순한 구조물에서 점차 재난피해로부터의 심리적 안정을 도모할 수 있는 안식처로서의 공간 조성에 노력해왔음을 알 수 있다. 이에 따라 종이파티션시스템에서는 종이관·허니콤패널·골판지시트·(면직물)막(커튼)을 결합한 형태로 임시주거에 대한 복합구조로서의 기능향상을 도모해왔다. 따라서 재난구조 가설건축과 관련해서 보면 시게루 반은 휴머니티(humanity)의 건축을 추구한다고 볼 수 있다. 이러한 측면은 건축의 생태성과 윤리라는 관점에서 그가 재료를 덜 사용하되 더 오래 쓰고 재활용될 수 있도록 디자인한다는 면에서 생태건축적 방식이라든지, 재난시 자원봉사 활동을 통한 건축가의 자발적인 사회적 기여 측면, 그리고 필요에 의하거나 자원봉사를 통해 개인이 직접 건축물을 짓게 함으로써 얻게 되는 자아실현의 성취감 등과 연계된다고 볼 수 있다. This study aims to make clear the cases and characteristics of temporary architecture for disaster relief which are very important to comprehend Shigeru Ban`s works. The conclusions of this study are as follows: Firstly, the influences from Shigeru Ban`s architectural careers and educations are ①the concerns for recycling of materials ②the experimental inclination ③the influence from pursuit of regional condition ④the influence from engineering including structure. Also, the background and cause of temporary architecture for disaster relief are derived from his vocational duty as an authentical architect. Secondly, the paper-tube and paper-partition-system of temporary architecture for disaster relief which have been developed by continuous experiments and creative ideas are based on the efficiency of economy and construction, environment-friendly architecture, refugees` regional environment. Specially, paper-partition-system is designed to compose private spaces as shelters that makes refugees feel easy. Finally, Shigeru Ban`s works are designed to pursuit the humanitarian architecture in relation to temporary architecture for disaster relief. These are stemmed from a method of ecological architecture which include recycling, from architect`s voluntary service for social contribution, and from self realization by building themselves`s houses.

한국형 기동헬기 내폭발성 만족을 위한 연료 벤트 시스템 설계개선

김정훈(Joung-Hun Kim),김창영(Chang-Young Kim),장중진(Joong-Jin chang),이말영(Mal-Young Lee),심대성(Dai-Sung Shim) 한국항공우주학회 2014 韓國航空宇宙學會誌 Vol.42 No.1

군용 헬리콥터는 적 지역에 침투 시 저공비행 및 침투비행으로 인해 적의 소형화기에 노출되면서, 피탄에 의한 항공기의 폭발 유발 가능성이 높다. 최근 군요구도에 있어서 피탄에 의한 폭발 환경에 대해서 생존성 확보를 위한 내탄 성능을 요구한다. 국내 최초의 군용 헬리콥터인 한국형기동헬기의 연료계통 요구도에도 적의 피탄에 대한 내폭발성을 요구하고 있으며, 요구도 충족을 위해 국내 최초로 탑재형 불활성 가스발생장치(OBIGGS)를 적용하였다. 개발단계에서 미흡하게 설계된 내폭발성과 연관된 Vent System을 개선하여 내폭발성 성능을 재평가하고 설계에 적합함을 입증하였다. Military helicopter is exposed to the enemy gun firing due to the low altitude flight of contour flight, hovering & nap of the Earth flight, therefore it has the high possibility to be exploded by the gun firing. Recently the Anti-ballistic requirement is required to get the high level of safety from gun firing in required operational capability. The first military utility helicopter of SURION has the Anti-ballistic requirement and explosion proof. In order to meet the requirement, OBIGGS is adopted for the first time in KUH. It is proven that Anti-Explosion capability is satisfied to requirement for improving vent system which was insufficiently designed in development period and related to Anti-explosion