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        Antioxidation and Cancer Cell (HT-29) Antiproliferation Effects of Rubus coreanus Miquel Bamboo Salt

        Xin Zhao,Jiale Song,Joug-Hynu Lee,So-Young Kim,박건영 대한암예방학회 2010 Journal of cancer prevention Vol.15 No.4

        Bamboo is used by Korean as an important medicinal material. It has many different medicinal efficacy from its stub, roots and leaves. Rubus coreanus Miquel (raspberry) is thought to contain compounds which are important towards having health benefits. Rubus coreanus Miquel bamboo salt sample was prepared and showed in vitro antioxidant, antimutagenic and anticancer effects in this study. The radical scavenging effect of Rubus coreanus Miquel bamboo salt on DPPH radical were tested. The Rubus coreanus Miquel bamboo salt sample revealed better scavenging activities on DPPH radical than the other salt samples at concentrations of 10 and 20%. Antimutagenic effects of Rubus coreanus Miquel Bamboo salt were investigated and compared with other salt samples. Though solar salt exhibited comutagenicty with MNNG, bamboo salt showed antimutagenicity against MNNG. The Rubus coreanus Miquel bamboo salt showed more increased antimutagenicity than the bamboo salt. MTT assay was carried out with the salts treated HT-29 cancer cells. The Rubus coreanus Miquel bamboo salt showed better anticancer activities (69%) than purple bamboo salt (44%) and solar salt (11%) at a concentration of 1%, and Rubus coreanus Miquel bamboo salt also exhibited better anticancer activities than other salt samples at the low concentration of 0.5% (p<0.05). The analysis of apoptosis related genes was carried out using RT-PCR to check the anticancer effect in the colon cancer cells and showed the same anticancer cancer effects as shown in MTT assay. From the experimental results, Rubus coreanus Miquel bamboo salt exhibited better health functional effects than common bamboo salts and solar salt. (Cancer Prev Res 15, 306-312,2010)

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