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Sergio Carvalho de Santana,Everaldo Silvino dos Santos,Raimundo Cosme da Silva Filho,Jorge dos Santos Cavalcanti,Jackson Araujo de Oliveira,Gorete Ribeiro de Macedo,Francine Ferreira Padilha 한국화학공학회 2014 Korean Journal of Chemical Engineering Vol.31 No.4
A mathematical model was developed to predict breakthrough curves during purification of the two chitosanasesfrom Metarhizium anisopliae by expanded bed adsorption, taking into account the axial dispersion of liquid andusing Streamline DEAE and SP XL adsorbents, anion and cation exchange resins, respectively. All the experimentswere performed without clarification (with cells) aiming at the reduction of unit operations in future projects of separationprocesses, thereby reducing capital and operating costs. Chitosanases are enzymes that hydrolyze the carbohydratechitosan, resulting in oligosaccharides that have many remarkable biological activities, such as anti-cancer, anti-HIVand antioxidant activities. The two adsorbents had similar performance in relation to hydrodynamics and mass transfer. The results of the parametric sensitivity analysis agree with the literature, and the model was validated with an averagehigh degree of fit (94.68%) between simulated and experimental data obtained in this work.