營農生活館 敎育에 關한 硏究

崔元凱,李宗玉 진주산업대학교 1980 論文集 Vol.18 No.-

The purposes of this study were to make clear the effects of the agricultural life education and to get a better education for the agricultral junior technial college student. Data for this study were collected from questsionnarie to the freshmen who had completed the agricultural life education for a week in 1979 and 1980. The following conclusions were drawn from the research findings: 1. There wre many students who would wished to be transferred to senior college or university as a motive of the entrance of junior technical college. A number of students was decreased to be transferred to senior college, and increased to be accomplished the purposes of agricultural junior technical college after the completion of the agricultural life education. 2. Most of students were much interested by the succesful stories of practical and real farm management and home management, the day's routine of life experiences, and several group activites such as group discussion, group recreation, candle fire ceremony etc. 3. Throughout the agricultural life education many students were much interested in farm life and farm settlement. 4. The effective results of agricultural life educbtion would be much increased by the educational supplement of the speciality and the professor's cooperation of each department. 5. The fruitful education would be expected in perfect harmony among guidance professors, instructors and students, and by good accommodations.

새마을 主産團地의 營農實態 比較硏究

徐大振,李宗玉,鄭鉉承,崔元凱,姜鎬允 진주산업대학교 1981 論文集 Vol.19 No.-

The purpose of this study were to find the actual condition of the co-operative group farming in Korea, and to improve the low income Saemaul villages by the adoption of good points in co-operative Group farming. There were so many co-operative farmings in Korea, but all of them were not so successful, Eight co-operative group farmings were chosen to investigate for the above purposes. (Table 1). The tendency of agricultural income was not always true. First of all, Co-op Poultry farm did not follow the order of agricultural income. Because there was an excellent leader who had eliminated the circulation expenses in process of the purchase of feeds and the sale of eggs in common, Second, Seachiodong flower were devived into two parts the cultural flower and the sale flower. The cultural flower meant mainly producing flowers and the sale flower mainly selling flowers to downtown shops in Seoul. The income per householder of the cultual flower was less than that of the sale flower. Consequently, sucessful Co-operative Group farmings had an able leader, and performed production and sale more reasonablly.

실험 방광암 모델에 있어서 Proliferating Cell Nuclear Antigen이 발현되는 세포들의 색상-화상 분석

이현무,이태수,김원재,차은종,송형근 충북대학교 의과대학 충북대학교 의학연구소 1993 忠北醫大學術誌 Vol.3 No.1

Proliferating cell nuclear antigen(PCNA)에 대한 단클론성 항체에 의해 면역조직화학적으로 염색된 세포들의 평가에 있어서 색상-화상 분석의 역할을 살펴보고자 하였다. BBN 투여 기간에 따라 Fisher 344 백서 방광에 정상 상피로부터 단순 과증식, 결절성 혹은 유두상 과증식 및 표재성 방광암에 이르는 순차적인 병리세포학적 변화가 발생함을 관찰한 후, 이를 색상-화상 분석과 육안적 분석을 통해 PCNA에 대한 평균 labeling index를 구한 결과 두가지 방법에서 모두 각 병변 사이에 통계적으로 유의한 차이를 보였으나, 색상-화상 분석을 이용하여 구한 평균 labeling index의 변이계수가 육안적 분석을 이용하여 구한 평균 labeling index의 변이계수에 비해 전 병변에 대하여 낮게 나타나, 종양화과정이 진행함에 따라 PCNA에 대한 평균 labeling index가 증가하고 평균 labeling index가 높을 수록 증식도가 더 큼을 나타낸다는 결과를 얻었으며, 색상-화상 분석을 이용하여 얻은 결과가 육안적 분석을 이용하여 얻은 결과보다 신뢰도가 더 높은 것으로 나타났다. 이와같은 면역조직화학염색에 대한 색상-화상 분석을 통한 세포역동학적 연구는 정량화 및 재현성의 향상을 통해 면역조직화학염색의 평가에 있어 보다 신뢰도가 높은 정보를 제공하여줄 것이며, 이를 통해 방광종양의 증식도 및 악성도를 예측하는데 중요한 역할을 할 것으로 생각된다. A study was carried out to define the role of color-image analysis in evaluation of immunohistochemically labeled cells using anti-PCNA/cyclin monoclonal antibody. Sequential cellular changes(normal, simple hyperplasia, nodular or papillary hyperplasia, and transitional cell carcinoma-Ta, T1) were observed in Fisher 344 rat urinary bladder depending on the duration of BBN administration. The mean PCNA labeling index obtained by color-image analysis and visual analysis in normal, simple hyperplasia, nodular or papillary hyperplasia and bladder tumor showed statistically significant difference in each histological group. But coefficients of variation of mean PCNA labeling index obtained by color-image analysis were lower than those using visual analysis in all lesions. Higher PCNA labeling indices indicated greater biological malignant potential, and mean labeling index in each histological group obtained by both color-image and visual analysis progressively increased with tumorigenesis, but the coefficient of variation with color-image analysis was lower than that with visual analysis in general, therefore, the evaluation of labeling indices obtained using color-image analysis was more reliable than that obtained using visual analysis. The data suggest that cell kinetic evaluation using color-image analysis may provide more reliable information to evaluate immunohistochemical staining in terms of quantitation and reproducibility and play an important role in predicting proliferative activity and malignant potential for bladder tumor.

컨테이너 터미널의 효율적 운영을 위한 의사결정지원시스템

김갑환,김홍배,윤원영,김종훈,권봉재,조지운 釜山大學校生産技術硏究所 1998 生産技術硏究所論文集 Vol.55 No.-

A decision support system is introduced for the efficient operation of port container terminals. It consists of ship planning subsystem, yard planning subsystem, resource management subsystem, real-time control subsystem, and simulation subsystem. The system is intended to support the planning process of the planners and the operation process of the administrators in the container terminals. It is assumed that the transfer crances and the yard tractor/trailers are used to handle containers in the marshaling yard. The system is explained in terms of the functions of each subsystem and the data flows among various subsystems.

TNF-α 유전자형과 방광암과의 관계

정필두,김은정,엄민식,서정원,윤석중,김종석,이상철,김원재 충북대학교 의학연구소 2001 忠北醫大學術誌 Vol.11 No.2

연구목적: TNF-α는 일부 종양의 종양화 과정과 관련이 있는 것으로 알려져 있다. 본 연구는 TNF-α 발현에 영향을 미치는 TNF-α 촉진자 -308 부위의 유전적 다형성이 방광암과 관련이 있는지 유무를 알고자 시행하였다. 대상 및 방법: 유전자 분석을 위하여 환자 113명 및 대조군 109명으로부터 혈액을 채취하여 genomic DNA를 분리한 후 PCR-RFLP 및 direct DNA sequencing을 통하여 TNF-α유전자의 다형성을 조사하여 방광암의 발생, 병기 및 분화도와 비교 검토하였다. 결과: TNF-α 촉진자 -308 부위의 유전형은 대조군에서는 GG형이 83.5%(90례 및 GA형이 16.5%(19례)로 관찰되었으며 AA형은 없었다. 환자군에서는 GG 형이 85.4%(97례), GA형 및 AA형은 각각 13.1%(15례)및 0.8%(1례)에서 관찰되었다. 두 군 모두에서 GG형이 가장 많이 나타났으며 다음으로 GA형을 보이고 AA형은 1례의 방광암 환자에서만 관찰되었다. -308부위의 경우도 두 군 사이에 유전자형의 차이는 없었다(p=0.259) 분화도별 분포를 보면 grade I이 20례, grade II가 49례, grade Ⅲ은 34례였고 병기별로 표재성인 경우가 90례였으며 침윤성은 14례였다. 분화도가 나빠질수록 GA형이 증가하였다(p=0.04). 그러나 병기와 TNF-α promoter -308부위의 유전자형 사이에는 유의한 상관 관계가 없었다(p=0.123). 결론: 방광암 환자의 혈액에서 GA genotype이 관찰되는 경우, 분화도가 나쁠 가능성이 매우 높기 때문에 좀 더 적극적인 치료와 세밀한 추적관찰을 함으로써 방광암으로 인한 사망과 암의 진행을 예방할 수 있을 것으로 기대한다. Purpose : Tumor necrosis factor-alpha (TNF-α) is involved in tumorigenesis of several cancers as an endogenous tumor promoter. The purpose of this study was to investigate whether genetic polymorphism of TNF-α promoter region (-308) was associated with human bladder tumor. Materials and Methods: The DNA from 113 and 109 respective blood samples of bladder tumor Patients and control group was analyzed by PCR-based restriction fragment length polymorphism (RFLP) and direct DNA sequencing methods to characterize the genetic polymorphism of -308 promoter region of the TNF-α gene in bladder tumor patients. We compared the association of bladder tumor with genetic Polymorphism of TNF-α promoter region(-308) in relation to the stage, grade, recurrence and progressio. Results : Eighty-six percents(97/113) of bladder tumor patients and 83.5% (90/109) of control group showed genotype GG at -308 region of TNF-α. Difference in genetic variations of TNF-α promoter (-308) did not exist between bladder tumor patients and control group(p=0.259). Tumor grade was significantly related to the GA genotype (p=0.04). The higher is the grade in bladder tumor, the more frequent was the GA genotype. Tumor stage, recurrence and progression were not significantly associated with genetic polymorphism of TNF-α promoter region (-308). Conclusion: The GA genotype of TNF-a promoter region (-308) had a significant impact on TNF-α production and was related to higher grade tumor compared to GG genotype. TNF-α serum levels in bladder tumor patients were significantly higher than controls. These data suggested that TNF-α might involve the tumorigenesis of the bladder rather than treatment or prevention of bladder tumor.

Development of a Monitoring System for Water-borne Bacteria by a Molecular Technique, PCR-RELP-sequence Analysis

이혜영,--,--,--,--,--,-- THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 2003 Journal of biomedical laboratory sciences Vol.9 No.3

Since water borne infection causes acute diseases and results in spread of diseases by secondary infection, the prevention is very important. Therefore, it is necessary to have a method that is rapid and effective to monitor pathogenic bacteria in drinking water. In this study, we employed a systematic method, Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) analysis, to develop an effective monitoring system for possible bacterial contaminants in drinking water. For this purpose, PCR primers were derived from 992 bp region of the 16s rRNA gene that is highly conserved through the different species of prokaryotes. To test whether the PCR primers designed are indeed useful for detecting all the possible microbial contaminants in the water, the primers were used to amplify 16s rRNA regions of different microbial water-borne pathogens such as E. coli, Salmonella, Yersinia, Listeria, and Staphylococcus. As expected, all of tested microorganisms amplified expected size of PCR products indicating designed PCR primers for 16s rRNA indeed can be useful to amplify all different microbial water-borne pathogens in the water. Furthermore, to test whether these 16s rRNA based PCR primers can detect bacterial populations present in the water, water samples taken from diverse sources, such as river, tap, and sewage, were used for amplification. PCR products were for then subjected for cloning into a T-vector to generate a library containing 16s rRNA sequences from various bacteria. With cloned PCR products, RFLP analysis was done using PCR products digested with restriction enzyme such as Hae Ⅲ to obtain species-specific RFLP profiles. After PCR-RFLP, the bacterial clones which showed the same RFLP profiles were regarded as the same ones, and the clones which showed distinctive RFLP profiles were subsequently subjected for sequence analysis for species identification. By this PCR-RFLP analysis, we were able to reveal diverse populations of bacteria living in water. In brief, in unsterilized natural river water, over 60 different species of bacteria were found. On the other hand, no PCR products were detected in drinking tap-water. The results from this study clearly indicate that the PCR-RFLP-sequence analysis can be a useful method for monitoring diverse, perhaps pathogenic bacteria contaminated in water in a rapid fashion.

MIPv6 환경에서 VoIP 서비스를 위한 통합 보안 메커니즘 제시와 요구사항 분석

서종운(Jong-Wun Seo),안태선(Tae-Sun Ahn),김지수(Ji-Soo Kim),강현국(Hyun-Kook Kahng) 한국정보과학회 2003 한국정보과학회 학술발표논문집 Vol.30 No.2Ⅲ

현재 인터넷 서비스의 근간을 형성하고 있는 IPv4의 가용 주소 공간의 고갈, 보안성의 결여, 그리고 멀티미디어 서비스를 위한 QoS(Quility of Service)의 필요성과 같은 요구사항을 바탕으로 차세대 인터넷 프로토콜(IPv6)로의 전환이 요구되고 있다. 본 연구 목적은 이러한 네트워크상의 이동 인터넷 환경에다 실시간 서비스를 제공할 수 있도록 SIP(Session Initiation Protocol)를 적용하여 통합 된 환경이 이전 보다 안전한 인터넷 정보 서비스를 제공할 수 있도록 보안 메커니즘을 적용 하였다. 네트워크 계층과 응용 계층의 이동성 관리 모델의 통합은 전체적인 시그널링 부하를 줄이고 지속적인 통신을 위한 빠른 핸드오프를 제공한다. 즉, 본 연구는 현재 Mobile IPv6 에서 보안상 취약점으로 나타나는 문제점 및 SIP 보안 고려사항 및 이동성을 해결하기 위해 제안되는 해결방안들을 분석하고 적합한 보안 메커니즘 적용 방안을 제안 하였다.

500MHz IF 로컬 신호용 저 페이즈 노이즈/저 전력 VCO

이종렬(Jong-Ryul Lee),김욱(Ook Kim),오창준(Chang-Jun Oh),권종기(Jong-kee Kwon),송원철(Wun-Chul Song),김경수(Kyung-Soo Kim) 대한전자공학회 1996 대한전자공학회 학술대회 Vol.1996 No.11

영상처리를 이용한 비접촉식 교량변위 측정 시스템

이종운(Jong-wun Lee),오주성(Ju-Sung Oh),권순덕(Soon-Deuk Kwon),곽종원(Jong-won Kwok) 대한전기학회 2005 정보 및 제어 심포지엄 논문집 Vol.2005 No.1

WC-Ni-Si-B₄C계 초경합금 제조 및 스테인레스 스틸과의 확산접합

원종운(Jong-Wun Won) 한국생산제조학회 2015 한국생산제조학회지 Vol.24 No.6