카드뮴이 마우스 뇌에서 아연의 항상성에 관여하는 유전자발현에 미치는 영향

박종안,여은영,남상훈,장봉기,이종화,김완종 한국환경독성학회 2004 환경독성보건학회지 Vol.19 No.4

Metallothionein (MT), a small protein molecule which can bind of release metal tons, is involved in the regulation of cellular metal homeostasis This study was investigated the accumulation of cadmium in blood, tissue (liver, kidney and brain), and the effect of cadmium on several key genes (MT-Ⅰ, MT-Ⅱ, ZnT-1) in zinc metabolism m the mouse Mouses weighing 20-25g were randomly assigned to control and cadmium treated group (Cd group) Cd group was intraperitoneally injected with cadmium 2, 4, 8 mg/kg and control group was administerd with saline Mouses of each group were sacrificed by decapitation 4 hours after the administration of cadmium Cadmium contents in blood, fiver, kidney and brain were increased by a dose-dependent manner Accumulation of cadmium was mainly occurred in liver and kidney Induction of MT-I and MT-11 protein was increased, but ZnT-1 expression was decreased to a dose-dependent manner by the treatment of 2~8mg/kg cadmium These results suggested that cadmium can be transported to brain and alter the expression of several key genes in zinc homeostasis.

카드뮴의 중추신경계 독성유발 기전

이종화,장봉기,박종안,박종영,김완종,우기민 한국환경독성학회 2004 환경독성보건학회지 Vol.19 No.3

Although numerous studies have shown that cadmium disturbs the normal biological processes in central nervous system the mechanism of toxicity is not well understood The present study has investigated the effect of cadmium on oxidative stress, Na+/K- ATPase activity and the aggregation of amyloid beta peptide(β-amylotd) in neuronal cell Ime, HT22 cell LC_(5) and LC_(50) of cadmium for HT22 cell resulted from MTT assay was 4 1 μM and 9 5 μM, respectively Cadmium(2 to 8 μM) dose-dependently increased the lipid peroxidation and decreased the content of glutathione Cadmium 4 μM showed a significant decrease in Na^(-)/K^(+) ATPase activity as compared with control group The aggregation of P-amyloid was accelerated in a dose-dependent manner by the treatment with 2 to 8 μM cadmium These results suggest that the neurotoxicity of cadmium can be mediated by the increase in oxidative stress and decrease in Na^(-)/K^(+) ATPase activity.

상수 원수에 따른 용존 유기물의 특성 평가

허준무,박종안,손부순,장봉기,이종화 한국환경과학회 1999 한국환경과학회지 Vol.8 No.3

This study was carried out to evaluate the characteristics of dissolved organics based on their origins, which were divided into two categories. The first group consisted of river, lake and secondary sewage treatment effluent, which were chosen as representative of their origins. The second group were artificial samples which were made of AHA(Aldrich humic acids) and WHA(Wako humic acids). Physicochemical characteristics, biological degradability and THMFP(trihalomethane formation potential) of the samples were analysed based on the AMWD(apparent molecular weight distribution). Large portion of dissolved organic carbon(DOC) in the river and lake samples was comprised of LMW(low molecular weight), while that of AHA and WHA was HMW(high molecular weight). The DOC of the lake was evenly distributed in the all range of molecular weight. The river, lake and secondary treated effluent have lower ultraviolet(UV) absorbance at 254nm, and have a higher amount of humic acids. Higher absorbance of humic acids means that aliphatic bond and benzenoid type components that absorb UV light were contained in these kind of humic acids. It was expected that lake sample was the most biodegradable in the different samples investigated, and in order of secondary sewage treatment effluent, river, WHA and AHA based on the result of determination of specific ultraviolet absorbance(SUVA). Biodegradability showed similar result except for ABA, while dissolved organics in the range of LMW decreased during the biodegradability test, and on the contrary those of HMW increased. Production of the SMPs(soluble microbial products) was observed during humicification of dissolved organics and the SMPs were in the range of 0.7∼5.5% of DOC which was the lowest value. Sample had a higher portion of HMW showed a higher production of the SMPs. THM formation was high in the samples containing HMW and similar tendency was shown in the THMFP(trihalomethane formation potential), except for WHA.

자동화학분석기 Cobas Integra를 이용한 화학검사측정에 대한 평가

이용화,정규영,박은주,김종원 대한임상병리학회 1997 대한임상병리학회지 Vol.17 No.5

철강 제조업체 근로자에서 효소면역측정법을 통한 B형 간염 바이러스 표지자 조사

이종영,강승원,하명화,김중구,배삼덕,김두희,남복동 大韓産業醫學會 1996 대한직업환경의학회지 Vol.8 No.2

포항 지역 한 철강 제조업에서 1995년 1월부터 10월 사이에 실시한 정기 건강진단을 받은 남자 근로자 11,523명을 대상으로 HBsAg 및 Anti-HBs, HBeAg 및 Auti-HBe은 EIA법으로, SGOT 및 SGPT는 반응속도법(IU)으로 검사하였다. 그리고 이들 대상자에서 교육정보, 근무 형태, 음주 및 흡연습관과 간기능 이상 여부를 조사하여 다음과 같은 결과를 얻었다. 1. B형 간염 표지자의 분포를 보면 HBsAg(+)/Anti-HBs(-)는 8.1%, HBsAg(-)/Anti-HBs(+)는 60.5% 그리고 HBsAg(+)/Anti-HBs는 0.1%이었으며, HBsAg의 양성율은 8.2%, Anti-HBs 양성율은 60.6%이었다. 2. 연령에 따라 HBsAg(+)/Anti-HBs(-)와 HBsAg(-)-HBs(+)가 유의하게 증가한 반면(p<0.05), 교육정도와 근무형태에 있어서는 통계적으로 유의한 차이가 없었다. 3. 음주율은 HBsAg(+)/Anti-HBs(-)에서 HBsAg(-)/Anti-HBs(-)와 HBsAg(-)/Anti-HBs(+)의 84.6% 및 85.7%에 비해 낮지만 73.9%나 되었고, 흡연율은 HBsAg(-)/Anti-HBs(+), HBsAg(+)/Anti-HBs(-) 및 HBsAg(-)/Anti-HBs(-)에서 54.3%, 53.7% 및 53.0%의 순서로 나타났다. HBsAg(+)/Anti-HBs(-)에서 간기능 이상자가 40.1%이었으나 HBsAg(-)Anti-HBs(-)와 HBsAg(-)/Anti-HBs(+)에서도 각각 21.9% 및 18.4%를 차지하였다. 4. HBsAg(+)에서의 e항원에 대한 표지자의 분포를 보면 HBeAg(+)/Anti-HBe(-)는 31.3%, HBeAg(-)/AntiHBe(+)는 55.0% 그리고 HBeAg(+)/Anti-HBe(+)는 1.6%이었고 HBeAg(+)/Anti-HBe(-)는 연령에 따라 감소하고 HBeAg(-)/AntiHBs(-)와 HBeAG(-)/AntiHBe(+)는 증가하였다. HBsAg 양성자중 HBeAg의 총 양성율은 32.9%로 연령이 증가할수록 유의하게 감소하였다. 본 연구는 근로자의 B형 간염 표지자 조사에 있어 Anti-HBs의 검사에서는 EIA 방법으로 실시해야 하는 근거를 제시하는 것이며, 철강산업 근로자에서는 B형간염 이외의 간장질환에도 관심을 가져야 할 것으로 보이며 음주 문제에 대한 대책마련이 시급히 이루어 져야 할 것으로 생각된다. A study for seroprevalence of Hepatitis B virus markers (HBsAg, Anti-HBs, HBeAg and Anti-HBe) by EIA method and the Liver Function Test(SGOT and SGPT) using kinetic method was carried out from January to October 1995 among male workers of a major iron and steel manufacturing company located in Pohang. The results were as follows; 1. The distribution of hepatitis B virus markers showed that the proportions of HBsAg(+)/Anti-HBs(-), HBsAg(-)/Anti-HBs(+) and HBsAg(+)/Anti-HBs(+) were 8.1%, 60.5% and 0.1%, respectively. The positive rate of HBsAg in all subjects was 8.2%, whereas that of anti-HBs was 60.6%. 2. The proportions of HBsAg(+)/Anti-HBs(-) and HBsAg(-)/Anti-HBs(+) increased significantly with age. While the distribution of hepatitis B virus markers exhibited no significant differences among the groups classified by educational background or work type. 3. The proportion of drinkers in HBsAg(+)/Anti-HBs(-)(73.9%) was significantly lower than those of HBsAg(-)/Anti-HBs(-) and HBsAg(-)/Anti-HBs(+) (84.6% and 85.7%, respectively). The proportions of smoking for the three groups of HBsAg(-)/Anti-HBs(+), HBsAg(+)/Anti-HBs(-) and HBsAg(-)/Anti-HBs(-) were 54.3%, 53.7% and 53.0%, respectively(p<0.01). The proportion of the subjects with abnormal liver function test in HBsAg(+)/Anti-HBs(-) was 40.1%, whereas those in HBsAg(-)/Anti-HBs(-) and HBsAg(-)/Anti-HBs(+) were 21.9% and 18.4%, respectively. 4. The distribution of e Ag markers among HBsAg(+) subjects showed that the proportions of HBeAg(+)/Anti-HBe(-), HBeAg(-)/Anti-HBe(+) and HBeAg(+)/Anti-HBe(+) were 31.3%, 55.0% and 1.6%, respectively. The proportion of HBeAg(+)/Anti-HBe(-) decreased significantly with age and those of HBeAg(-)/Anti-HBe(-) and HBeAg(-)/Anti-HBe(+) increased with age. The positive rate of HBeAg among HBsAg(+) subjects was 32.9%. In conclusion, as a screening method, EIA is recommended to test hepatitis B virus markers, especially when examining Anti-HBs more sensitively. For the workers of the iron and steel manufacturing company studied, liver diseases caused by other than hepatitis B should be given a special care including an education avoiding alcohol drinking.

기존 콘크리트교량의 비파괴시험에 관한 연구

김종인,최영화,윤인무 大邱大學校 科學技術硏究所 1998 科學技術硏究 Vol.5 No.1

An existing concrete structure has been generally received serious environmental affects after construction, and thus the strength prediction will be different from normal structures. In particular, An old existing bridge would be more difficult to do because of uncertainties of construction history. This paper has examined degrees of erosion about concrete solidity of decrepit bridges due to long using and problems of non-destructive tests. In this study, the concrete solidity of all decrepit bridges are appeared generally many deficient in the strength comparing with the standard of design and it might be caused by erosion of concrete, carbonation, poor quality of construction, heavy traffic and so on.

대구·경북지역 콘크리트용 부순모래의 품질조사

최영화,김종인,전성태 大邱大學校 科學技術硏究所 1998 科學技術硏究 Vol.5 No.4

This study is aimed for investigating the quality of crushed sand, manufactured in Taegu kyungpook region, random sampled according to the quality regulation of crushed sand for concrete, and analyzing application as a fine aggregate for concrete. By the test results, the FM of crushed sand is average 3.51 and appears very larger than that of river sand, the specific gravity of crushed sand is larger than that of river sand and satisfies the K.S regulation that more than 2.5, and the amount of material finer than NO.200 sieve in crushed sand appears average 4.9%, and satisfies the K.S regulation that less than 7%. The void ratio in crushed sand appears smaller than that of river sand, the unit weight of crushed sand appears larger than that of river sand, and the solid volume ratio and the net shape ratio of crushed sand satisfy the K.S regulation that more than 53%. After all, it is considered that manufactured crushed sand would be sufficiently used as a fine aggregate for concrete. Futhermore, if possible, the combined sand of river and crushed sand would be very effective in the aspect of economical efficiency and quality of concrete.

아산시 종량제 쓰레기 봉투에 배출된 생활폐기물 성상의 주택유형 및 계절적 특성

박종안,한성현,손부순,이종화,장봉기,염윤기 순천향대학교 기초과학연구소 2002 순천향자연과학연구 논문집 Vol.8 No.2

This study was investigated physicochemical characteristics of residential wastes in using volume-base charge system of disposal bag. It examined from August 31, 1999 to March 30, 2000 to Asan city. It divided residing conformation to singleness house, few stories apartment (below 5 layers), high-rise apartment (more than 6 layers). Samples were collected 20ℓ volume bag by each 3 volume-base charge system of disposal 1 times by season in total 27 areas. Total 108 samples were analyzed characteristics of living wastes and three ingredients (moisture, volatile solids, ash) by season and house types. 1. Mean bulky density in the volume-base charge system of disposal envelope showed by 0.206 tons/㎥. Bulky density in high-rise apartment was the lowest by 0.183 tons/㎥ by house types, and few stories apartment and singleness house appeared similarly by each 0.217 tons/㎥, 0.218 tons/㎥. 2. Weight by season of waste in volume-base charge system of disposal bag was the highest in winter and the lowest in spring. 3. Occurrence amount by composition ratio about total weight combustibility was 93.3%, and incombustibility was 2.8%, and recycling was 4.0%. In physical composition, the compositional weight fraction was food, 47.9%, paper, 27.8%, vinyl & plastics, 10.7%, textile 3.4%. 4. In combustible wastes, the compositional weight fraction was food, 51.3%, paper, 29.8%, vinyl & plastics, 11.4%, textile, 3.7%, In combustible wastes, the compositional weight fraction was glass & ceramics, 85.3%, and soil & others, 9.1%, and metal was 5.6%. Glass bottles of recycling were 36.8%, plastics kind 24.6%, and can kind was 3.8%. 5. Average moisture content of combustible wastes appeared 28.60±22.35% and moisture content of food kind highest by 45.29%. Ash content was few amount of 6.55%. Volatile solids amount was high by 64.84% but vinyl & plastics was the highest by 78.05%, and food was the lowest by 46.83%. 6. Three ingredient findings moisture contents by season of combustible waste were the highest in summer and the lowest in winter (p<0.01).

격자구조를 이용한 단순지지 슬래브의 근사해석

김종인,최영화,김인수 대구대학교 과학기술연구소 1995 科學技術硏究 Vol.2 No.1

This study is aimed to seek alternative grillage method of simply supported slab which is subjected to distributed and concentrated load. The plate theory is summarized, and the exact solutions of structural element concerned are derived. The approximate grillage solutions of deflection are compared with the exact solutions at some reference points. The results are as follows 1. If the square plate is partitioned as four pieces, the error between the exact and approximate solution is 28% when distributed load is acted and that is 57% when concentrated load is done, but they are rather large value. 2. If the plate is partitioned as eight pieces and more, the error between the exact and approximate solution is 9∼16% when distributed load is acted and that is 9∼22% when concentrated load is done.

보건관리대행 기관의 사업장내 진료에 대한 근로자 요구 및 실시 의향

원종욱,송재석,정선아,박화미,노재훈 大韓産業醫學會 2001 대한직업환경의학회지 Vol.13 No.1

목적 : 본 연구에서는 보건관리대행 사업에 있어 사업장내 진료에 대한 수요와 보건관리대행기관의 방문진료 제공에 대한 의향을 조사하여 사업장내 진료에 대한 욕구를 파악하고, 이를 토대로 사업장내 진료에 대한 기초적인 자료를 제공하는데 목적이 있다. 방법 : 근로자들의 의료이용 행태와 방문진료에 대한 요구도를 파악하기 위해 167개 보건관리대행 사업장의 사업주와 보건담당자, 그리고 1,350명의 근로자에게 설문 조사를 실시하였고, 사업주 121명(72.5%), 보건담당자 145명(86.8%), 근로자 816명(60.5%)이 응답하였다. 일차진료의 제공 가능성을 파악하기 위해서 전국의 66개 보건관리대행기관의 의사와 간호사 및 행정책임자에게 우편 설문조사를 실시하여 의사 31명 (47.0%), 간호사 38명(57.6%) 및 행정책임자 34명(51.5%)이 응답하였다. 결과 : 보건관리대행 사업을 수행하고 있는 의사의 87%와 간호사의 94%가 보건관리대행 업무를 담당하면서 많은 근로자들로부터 투약 등 진료를 요구받고 있었고, 100 %의 간호사와 행정책임자 94%, 의사의 76 %가 보건관리대행 사업에서 진료를 수행하는 것이 필요하다고 생각하고 있었다. 한편사업주의 85 %, 보건관리 담당자 86 %와 근로자81 %가 보건관리대행기관에서 사업장내 진료를 시행 할 때 이를 수용하겠다고 응답하였다.결론 대다수의 근로자와 사업주가 보건관리대행기관의 진료를 요구하고 있었고, 보건관리대행 기관의 의사와 간호사가 사업장에서의 진료를 수용할 의향이 있고, 직업성 뇌심혈 관계질환의 예방 등 필요성이 인정된다면 보건관리대행 의사의 사업장내 진료를 인정해야 한다고 생각된다. Objectives : This study was performed to survey worker's demands for worksite medical care and agency's intention for offering worksite medical care in Group Health Service Agency. Methods : One study subjects were employers, workers and office personnels in 167 factories. They were asked for medical care utilization behavior and worksite medical care demand. 121 employers(72.5%), 145 office personnels(86.8%) and 816 workers(60.5%) answered the questionnaire. The other subjects were doctors, nurses and administrators in 66 Group Health Service Agencies nationwide. They were asked for intention of offering worksite medical care in Group Health Service Agency through the mail survey. 31 doctors (47.0%), 38 nurses (57.6%) and 34 administrators answered the questionnaire. Results : 87 percentage of doctors and 94 percentage of nurses had experiences of being requested to prescribe and laboratory examination. All nurses, 94 percentage of administrators and 76 percentage of doctors thought that it was necessary to give medical treatment at worksite. Also in worksite 85 percentage of employers, 86 percentage of office personnels and 81 percentage of workers answered that they accepted the worksite medical care when it would be given. Conclusions : Worksite medical care should be permitted because almost of all workers and employers requested worksite medical care, and doctors and nurses in Group Health Service Agency were willing to give worksite medical care and there was necessity to prevent occupational cerebrovascular disease.