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김종식,황선도 한국수산학회 2003 한국수산과학회지 Vol.36 No.6
Spatial distribution and biomass of commercial shellfish were investigated in the Saemangeum tidal flat on the west coast of Korea during August 1999. Thirteen species of shellfish were collected. Major dominant species of the stydy area were Umbonium thomasi with a density of 6.7 ind. m^(-2) (38.2%), Mactra veneriformis with 6.6 ind. m^(-2) (37.4%) and Reticunassa festiva with 1.3 ind. m^(-2) - 17.2%). Commercially important species such as M. veneriformis (90.7 g·m^(-2), 69.3%), M. lusoria (15.6 g·m^(?) , 11.9%), C. sinensis (8.7 g·m^(-2), 6.7%) were dominant in total weight. Most of the shellfish were distributed between the mid-littoral and sub-littoral zones, but C. sinensis and L. flexuosa were distributed mainly in the polluted areas of the supra-littoral zone. The spatial distribution pattern of each shellfish species generally resembles the Mangyeong and Dongjin estuary. Biomass of commercially important species, M. veneriformis, M. lusoria, C. sinensis and Ruditapes philippinarum in the tidal flat (ca. 850 ha) was estimated to be 774, 135, 67 and 10 tons, respectively.
Jang, In Kwon,Kim, Jong‐,Sheek,Kim, Bong‐,Rae,Meng, Xian‐,Hong Blackwell Publishing Ltd 2011 Aquaculture research Vol.42 No.12
<P><B>Abstract</B></P><P>In the present study, we used TaqMan real‐time polymerase chain reaction to quantify and compare infection of white spot syndrome virus (WSSV) with shrimp production of <I>Fenneropenaeus chinensis</I> cultured in outdoor ponds along the west coast of the South Korea. In 2007, a total of 60 specimens in summer and 116 specimens in autumn were collected from 12 growing‐out ponds and 12 harvest ponds respectively. Pond harvest data were obtained from farmers. Of the summer samples, all specimens were WSSV positive, with a wide range of 12.4–7.0 × 10<SUP>7</SUP> (mean 7.5 × 10<SUP>6</SUP>) copies ng<SUP>−1</SUP> DNA; shrimp production was 1.7 metric tonnes per hectare (mt ha<SUP>−1</SUP>). Of the 116 autumn‐sample specimens, 81 (69.8%) were WSSV positive; WSSV infection had been decreased dramatically, to 0–7.2 (mean 3.5) copies ng<SUP>−1</SUP> DNA. Shrimp production of autumn ponds was 2.1 mt ha<SUP>−1</SUP>. Statistical analysis indicated that the difference in WSSV infections detected in summer and autumn was highly significant (<I>P</I><0.01). In summer, seven ponds (58.3%) with low‐WSSV infection loads (0–1000 WSSV copies ng<SUP>−1</SUP> DNA) had shrimp production of 2.7 mt ha<SUP>−1</SUP>; the others had shrimp production of only 0.2 mt ha<SUP>−1</SUP>. The mean shrimp production between the two infection levels showed a highly statistically significant difference (<I>P</I><0.01).</P>
한국 서해산 살조개 Protothaca jedoensis의 연령과 성장
김지현(Ji-Hyun Kim),김종식(Jong-Sheek Kim),김용호(Yong-Ho Kim),정의영(Ee-Yung Chung),류동기(Dong-Ki Ryu) 군산대학교 수산과학연구소 2004 군산대학교 수산과학연구소 연구논문집 Vol.5 No.-
Age and growth of the the Jedo venus clam, Protothaca jedoensis were investigated using samples from the subtidal zone of Boryeong, Chungcheongnam-do, Korea from January to December, 1999. The relationship between shell height and ring radius in each group was expressed as a regression line. Therefore, it could be recognized that there is a correspondence in the formation of each ring. Based on the monthly variation of the marginal index of the shell, it was suggested that the annual ring mark formation occurred during the period of February to March once a year. The relationship between .shell height (SH) and total weight (TW) was represented by the non-linear equation: TW = 0.0007 SH28919, and also in the relationship between shell length (SL) and shell height (SH), shell height (SH) and shell width (SW) were represented by the linear equations: SL=1.1 067SH+ 1.778, SW=0.6758SH-0.9824. Growth curves for shell height and total weight fitted to the von Bertalanffy equation were expressed as: SHt = 81.546(1-e-0.176(1+0.381)), TWt = 227.65(1-e-0.176(1+0381)2.8919.
( In Kwon Jang ),( Kannan Suriakala ),( Jong Sheek Kim ),( Xian Hong Meng ),( Tae Jin Choi ) 한국미생물 · 생명공학회 2011 Journal of microbiology and biotechnology Vol.21 No.11
A highly sensitive and specific TaqMan real-time PCR was used to quantify hepatopancreatic parvovirus (HPV) type III infections in wild broodstocks and hatcheryreared postlarvae (PL) of Fenneropenaeus chinensis. Totals of 159 and 162 wild brooders from three locations were captured, and 140 and 180 PL were obtained from seven and six commercial hatcheries in 2007 and 2008, respectively. Among the three wild broodstock groups from 2007, only 1 group showed HPV infection and 3.2% of 159 brooders were positive for HPV infection. In 2008, HPV infections were observed from all three wild broodstock groups with 1.93×10(4) copies/mg tissue of pleopods. Of 162 brooders, 26.6% were positive for HPV infection. No PL from the two hatcheries collected in 2007 showed HPV infection, and PL from the rest of the five hatcheries had up to 1.74×10(6) copies/ng of DNA, and PL from three hatcheries showed HPV infections with over 1,000 copies/ng of DNA. The PL from all seven hatcheries collected in 2008 showed up to 2.10×10(5) HPV copies/ng of DNA. PL from two hatcheries showed less than 100 copies/ng of DNA, but PL from the rest of the hatcheries showed HPV infections with over 1,000 copies/ng of DNA. These results show that HPV type III is widely distributed in Korea in addition to previously reported HPV type I, and they can be effectively detected by type-specific real-time PCR.
Jang, In-Kwon,Gopalakannan, Ayyaru,Suriakala, Kannan,Kim, Jong-Sheek,Kim, Bong-Rae,Cho, Yeong-Rok,Meng, Xian-Hong,Seo, Hyeong-Chul The Korean Society of Fisheries and Aquatic Scienc 2008 Fisheries and Aquatic Sciences Vol.11 No.4
Viral diseases are major emerging problems of shrimp that have affected the production, and even complete losses for shrimp farms. In this study, we developed a sensitive TaqMan real-time PCR method to quantify white spot syndrome virus (WSSV) and hepatopancreatic parvovirus (HPV) in the shrimp and pond water in which fleshy shrimp, Fenneropenaeus chinensis, and Pacific white shrimp, Litopenaeus vannamei, are reared. WSSV and HPV in pond seawaters ranged from $1.65{\times}10^3$ to $2.43{\times}10^9$ and from 0 to $4.43{\times}10^5$ copies/L of seawater, respectively. Of 20 ponds analyzed, all pond water and shrimp were positive for WSSv. L. vannamei showed higher susceptibility to WSSV than F chinensis. HPV was detected only in the pond water for F chinensis. In shrimp tissue, however, HPV was found in both species, with 23-times higher infection rate in F chinensis than L. vannamei. The total bacterial counts in the pond water ranged from $2.23{\times}l0^3$ to $1.98{\times}l0^5\;CFU/mL$. The variations in total bacterial count for each pond appeared to correlate to the variations of the WSSV load. Statistical analysis indicated that there was no significant difference (P>0.05) between the WSSV load in pond water and shrimp, and there was no relationship between total bacterial load and viral load in the pond water. However, a significant difference (P<0.01) was found between HPV load and L. vannamei and F chinensis pond water.