Comparative analysis of fat and muscle proteins in fenofibrate-fed type II diabetic OLETF rats: the fenofibrate-dependent expression of PEBP or C11orf59 protein

( Jong Ryeal Hahm ),( Jin Sook Ahn ),( Hae Sook Noh ),( Seon Mi Baek ),( Ji Hye Ha ),( Tae Sik Jung ),( Yong Jun An ),( Duk Kyu Kim ),( Deok Ryong Kim ) 생화학분자생물학회 (구 한국생화학분자생물학회) 2010 BMB Reports Vol.43 No.5

Fenofibrate, an agonist of PPARα, plays an important role in activating many proteins catalyzing lipid metabolism, and it also has a considerable effect on improvement of insulin sensitivity in the diabetic condition. To investigate fenofibrate- dependent expression of peripheral tissue proteins in diabetes, we analyzed whole muscle or fat proteins of fenofibrate-fed OLETF rats, an animal model of type II diabetes, using 2-dimensional gel electrophoresis. We found that many proteins were specifically expressed in a fenofibrate-dependent manner in these diabetic rats. In particular, a functionally unknown C11orf59 protein was differentially expressed in the muscle tissues (about 5-fold increase) in fenofibrate-fed OLETF rats as compared to control rats. Additionally, the signal proteins phosphatidylethanolamine binding protein and IkB interacting protein were differentially regulated in the fenofibrate-treated adipose tissues. We suggest here that these proteins might be involved in controlling lipid or carbohydrate metabolism in diabetes via PPARα activation. [BMB reports 2010; 43(5): 337-343]

RKIP phosphorylation–dependent ERK1 activation stimulates adipogenic lipid accumulation in 3T3-L1 preadipocytes overexpressing LC3

Hahm, Jong Ryeal,Ahmed, Mahmoud,Kim, Deok Ryong Elsevier 2016 Biochemical and biophysical research communication Vol.478 No.1

<P><B>Abstract</B></P> <P>3T3-L1 preadipocytes undergo adipogenesis in response to treatment with dexamethaxone, 1-methyl-3-isobutylxanthine, and insulin (DMI) through activation of several adipogenic transcription factors. Many autophagy-related proteins are also highly activated in the earlier stages of adipogenesis, and the LC3 conjugation system is required for formation of lipid droplets. Here, we investigated the effect of overexpression of green fluorescent protein (GFP)-LC3 fusion protein on adipogenesis. Overexpression of GFP-LC3 in 3T3-L1 preadipocytes using poly-<SMALL>L</SMALL>-lysine-assisted adenoviral GFP-LC3 transduction was sufficient to produce intracellular lipid droplets. Indeed, GFP-LC3 overexpression stimulated expression of some adipogenic transcription factors (e.g., C/EBPα or β, PPARγ, SREBP2). In particular, SREBP2 was highly activated in preadipocytes transfected with adenoviral GFP-LC3. Also, phosphorylation of Raf kinase inhibitory protein (RKIP) at serine 153, consequently stimulating extracellular-signal regulated kinase (ERK)1 activity, was significantly increased during adipogenesis induced by either poly-<SMALL>L</SMALL>-lysine-assisted adenoviral GFP-LC3 transduction or culture in the presence of dexamethasone, 1-methyl-3-isobutylxanthine, and insulin. Furthermore, RKIP knockdown promoted ERK1 and PPARγ activation, and significantly increased the intracellular accumulation of triacylglycerides in DMI-induced adipogenesis. In conclusion, GFP-LC3 overexpression in 3T3-L1 preadipocytes stimulates adipocyte differentiation via direct modulation of RKIP-dependent ERK1 activity.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Overexpression of GFP-LC3 in 3T3-L1 cells produces intracellular lipid droplets. </LI> <LI> SREBP2 is highly activated in preadipocytes transfected with adenoviral GFP-LC3. </LI> <LI> RKIP phosphorylation at serine 153 is significantly increased during adipogenesis. </LI> <LI> RKIP knockdown promotes ERK1 and PPARγ activation during adipogenesis. </LI> <LI> RKIP-dependent ERK1 activation increases triacylglycerides in adipocytes. </LI> </UL> </P>

Alpha-lipoic acid attenuates adipocyte differentiation and lipid accumulation in 3T3-L1 cells via AMPK-dependent autophagy

Hahm, Jong Ryeal,Noh, Hae Sook,Ha, Ji Hye,Roh, Gu Seob,Kim, Deok Ryong Elsevier 2014 Life sciences Vol.100 No.2

원저 및 증례 : 조기에 발견된 횡문근융해를 동반한 전격성 제1형 당뇨병 1예

백종하 ( Jong Ha Baek ),이경주 ( Kyeong Ju Lee ),함종렬 ( Jong Ryeal Hahm ),정정화 ( Jung Hwa Jung ),김수경 ( Soo Kyoung Kim ),정순일 ( Soon Il Chung ),정태식 ( Tae Sik Jung ) 대한당뇨병학회 2010 임상당뇨병 Vol.11 No.1

Fulminant type 1 diabetes is characterized by an abrupt onset, severe diabetic ketoacidosis at diagnosis and a relatively low HbA1c. Rhabdomyolysis is not uncommonly combined with diabetic ketoacidosis or hyperglycemic hyperosmolar coma. Rhabdomyolysis increases the development of acute renal failure and mortality of the patients with hyperglycemic diabetic emergency. A 34-year-old man presented with 2-days duration of oliguria, vomiting and lethargy. Formerly, he developed polyuria and flu-like symptoms such as generalized myalgia and headache for 7 days. Initial investigation showed metabolic acidosis (arterial pH 7.093, bicarbonate 5.1 mmol/L), severe hyperglycemia (glucose 1,576 mg/dL), a relatively low HbA1c (7.1%) and acute renal failure (blood urea nitrogen 77 mg/dL and creatinine 4.4 mg/dL). Both serum and 24-hour urine C-peptide levels were checked at very low range and islet autoantibody tests were all negative. Serum creatinine kinase was elevated to 2,397 U/L and urine myoglobin test was positive. The patient recovered from acute renal failure and rhabdomyolysis after fluid and insulin therapy. He discharged on admission 17th days and controlled diabetes by self injection of basal and bolus insulin. (Korean Clinical Diabetes J 11:79-85, 2010)

Changes in Serum Electrolytes, ECG, and Baroreflex Sensitivity during Combined Pituitary Stimulation Test

Kim, Sungsu,Kwak, Choong Hwan,Jung, Jaehoon,Baek, Jong Ha,Jung, Jung Hwa,Park, Ki-Jong,Kim, Kyongyoung,Kim, Soo Kyoung,Kang, Dawon,Hahm, Jong Ryeal Hindawi 2018 BioMed research international Vol.2018 No.-

<P>The mechanisms by which hypoglycemia increases cardiovascular mortality remain unclear. The aim of the study is to investigate changes in serum electrolytes, norepinephrine concentrations, electrocardiography, and baroreflex sensitivity (BRS) and associations between corrected QT (QTc) intervals and the changes in serum electrolytes during combined pituitary stimulation test (CPST). We recruited the subjects who were admitted to the Gyeongsang National University Hospital to undergo CPST between September 2013 and December 2014. Participants were 12 patients suspected of having hypopituitarism. Among 12 patients, cardiac arrhythmia in two patients occurred during hypoglycemia. There were significant differences in serum levels of potassium (<I>P</I> < 0.001), sodium (<I>P</I> = 0.003), chloride (<I>P</I> = 0.002), and calcium (<I>P</I> = 0.017) at baseline, hypoglycemia, and 30 and 120 minutes after hypoglycemia. Also, there was a significant increase in heart rate (<I>P</I> = 0.004), corrected QT (QTc) interval (<I>P</I> = 0.008), QRS duration (<I>P</I> = 0.021), and BRS (<I>P</I> = 0.005) at hypoglycemia, compared to other time points during CPST. There was a positive association between QTc intervals and serum sodium levels (<I>P</I> < 0.001) in 10 patients who did not develop arrhythmia during CPST. This study showed that there were significant changes in serum levels of potassium, sodium, chloride, and calcium, as well as heart rate, QTc interval, QRSd, and BRS during CPST. It was revealed that QTc intervals had a significant association with concentrations of sodium.</P>

비접지계통 중성점이동 현상 분석

이종훤(Jong-Hweon LEE),전명렬(Myeong-Ryeal JEON),최한열(Han-Yeol CHOI) 대한전기학회 2009 대한전기학회 학술대회 논문집 Vol.2009 No.7

전력계통의 비접지 계통에 지락 고장이 발생하면 중성점 이동으로 고장이 발생하지 않은 건전상의 전위가 상승한다는 사실은 누구나 다 알고 있다. 이는 전기적으로 균형을 이루고 있는 계통에 지락 고장으로 상간 불균형이 초래되기 때문인데 보통 비접지 계통의 보호는 1상 지락 시는 고장전류가 거의 없기 때문에 영상 과전압을 이용한 한시 보호계전 방식으로, 2중 접지 시는 과전류 계전방식으로 보호 하고 있다. 그런데 고장전류가 없어 상대적으로 파급이 적을 것 같은 1상 지락고장 시 건전 상에 발생한 과전압이 또 다른 고장을 유발, 더 큰 고장으로 진전되어 많은 복구비용을 발생하게 하거나 예상치 못한 현상을 일으켜 고장원인을 분석하고 처리하는데 어려움을 초래하기도 한다. 본 논문은 발전소 비접지 계통에서 발생하는 중성점이동 현상에 대해서 논하고자 한다.

靑少年 非行의 變化課程 分析

김성렬,김종두,박영규 서원대학교 교육대학원 2000 교육논총 Vol.4 No.-

This study aimed to reveal various type on changing process of juvenile delinquency and deviant behaviors in precess of rapid social change. Five theories of the approaches were adopted: anomie theory, subculture theory, social control theory, labelling theory, social disorganization theory. Based on the theoretical assumptions that become a juvenile delinquent is a process which consists of consecutive stage and through which degree of delinquency is progressively advancing, the following questions were explored in this thesis: how take the preventive measures and changing process of juvenile delinquent, The major finding of this study are as follows: First, it appeared that the increasing rate of the juvenile delinquent is higher then the total crime rate. Second, it appeared that a violent crime - murder.'robber'. violation, arson - is the highest in the juvenile delinquent. Third, it appeared that woman is higher than man in the crime rate of juvenile delinquent. Fourth, it appeared that the student delinquent is steadily increased. Fifth, students had a large percentage of entertainment costs in selfishness desire, happen highest rate the crime accidentally. According1y, a rapid increase in student delinquency, resulting from maladjustment, value conflict and the tendency of forming a gang with rough acts were noticed. Because the student crime of whole juvenile delinquency had continuously increased 61.8 % in 1998. Most juvenile delinquencies seems to be attributed to the lack of sufficient individual talent. educational experience and financial background. These problems are leading the youths, who haute not been admitted into right course of making success to the nicious cycle of juvenile delinquency and deviant behavior as a result of this study. On the contrary, because juvenike delinquencies are closely bound up with social morality, it is terriblt significant to recover social justice more than anything else.

Assessment of glycemic control in patients with type 2 diabetes mellitus treated with metformin–sulfonylurea combination: Results of a multicenter, cross-sectional, observational study in Korea

Kim, Sin Gon,Hahm, Jong Ryeal,Kim, Duk Kyu,Cho, Sung Rae,Choi, Dong Seop John Wiley & Sons Ltd 2015 Journal of diabetes investigation Vol.6 No.3

<P><B>Aims/Introduction</B></P><P>To assess the current status of glycemic control in patients with type 2 diabetes treated with a combination of metformin and sulfonylurea for >3 months, as measured by glycosylated hemoglobin (HbA1c).</P><P><B>Materials and Methods</B></P><P>Data on patient demographics, diabetic complications, HbA1c, fasting plasma glucose (FPG) and type of treatment were collected in this multicenter, cross-sectional, non-interventional study.</P><P><B>Results</B></P><P>From April 2008 to February 2009, 5,628 patients were recruited from 299 centers in Korea. Patients characteristics (mean ± SD) were as follows: age 58.4 ± 10.8 years, duration of diabetes 6.1 ± 4.7 years, body mass index 24.7 ± 2.9 kg/m<SUP>2</SUP>, HbA1c 7.77 ± 1.22%, FBG 147.4 ± 46.5 mmol/L and FPG 164.0 ± 54.3 mmol/L. The most common diabetic complication was neuropathy (22.5%), followed by retinopathy (18.3%) and microalbuminuria (16.1%). Just 1,524 (27.1%) patients achieved HbA1c ≤7%. A higher number of patients (32.6%) treated by endocrinologists achieved HbA1c ≤7% than those treated by internists (24.4%) and primary care physicians (23.2%). In multivariate analyses, diabetic retinopathy (odds ratio 0.455, 95% confidence interval 0.341–0.606), nephropathy (odds ratio 0.639, 95% confidence interval 0.43–0.949), diabetes for ≥5 years (odds ratio 0.493, 95% confidence interval 0.4–0.606) and older age added by 1 year (odds ratio 1.019, 95% confidence interval 1.01–1.029) was significantly associated with achieving target HbA1c. In addition, treatment by endocrinologists rather than internists significantly increased chances of achieving target HbA1c (odds ratio 1.417, 95% confidence interval 1.146–1.751).</P><P><B>Conclusions</B></P><P>The majority of patients with type 2 diabetes in Korea had inadequate glycemic control, despite receiving a combination of metformin and sulfonylurea.</P>

A Case of Hyperglycemic Hyperosmolar State Associated with Graves' Hyperthyroidism: A Case Report

Moon, Sung Won,Hahm, Jong Ryeal,Lee, Gyeong-Won,Kang, Mi Yeon,Jung, Jung Hwa,Jung, Tae Sik,Lee, Kang Wan,Jung, Kyoung Ah,Ahn, Yong Jun,Kim, Sunjoo,Kim, Me Ae,Kim, Deok Ryong,Chung, Soon Il,Park, Myoun The Korean Academy of Medical Sciences 2006 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.21 No.4

<P>Hyperglycemic hyperosmolar state (HHS) is an acute complication mostly occurring in elderly type 2 diabetes mellitus (DM). Thyrotoxicosis causes dramatic increase of glycogen degradation and/or gluconeogenesis and enhances breakdown of triglycerides. Thus, in general, it augments glucose intolerance in diabetic patients. A 23-yr-old female patient with Graves' disease and type 2 DM, complying with methimazole and insulin injection, had symptoms of nausea, polyuria and generalized weakness. Her serum glucose and osmolarity were 32.7 mM/L, and 321 mosm/kg, respectively. Thyroid function tests revealed that she had more aggravated hyperthyroid status; 0.01 mU/L TSH and 2.78 pM/L free T3 (reference range, 0.17-4.05, 0.31-0.62, respectively) than when she was discharged two weeks before (0.12 mU/L TSH and 1.41 pM/L free T3). Being diagnosed as HHS and refractory Graves' hyperthyroidism, she was treated successfully with intravenous fluids, insulin and high doses of methimazole (90 mg daily). Here, we described the case of a woman with Graves' disease and type 2 DM developing to HHS.</P>

성인 여자에서 발견된 뇌하수체 전엽 무형성증 1예

정태식,함종렬,이강완,정정화,김수희,하종,조활석,정순일 대한내분비학회 2002 Endocrinology and metabolism Vol.17 No.2

Dear Author, You have used abbreviations that will need to be defined in the main paper, i.e. PIT1, PROP1 and MRI. This is just for your advice. Pituitary agenesis is an uncommon cause of panhypopituitarism. It has been proposed that breech delivery, or birth trauma, is a major factor causing pituitary agenesis. Recent studies have suggested that genetic defects in the PIT1 or PROP1 gene might be involved in the pathogenesis of pituitary agenesis. In this case we report on the diagnosis of a 33-years old female patient with- growth retardation and sexual infantilism. We diagnosed anterior pituitary hormones deficiencies, with the exception of adrenocorticotropic hormone, by a combined pituitary stimulation test. We observed pituitary agenesis using sella MRI. Involvement of the PIT1 or PROP1 genes in this case remains to be determined. Here we report a case of pituitary agenesis found in an adult woman together with a brief review about this disease entity