韓牛 末梢血液 淋巴球의 在來山羊 赤血球 Rosette形成

宋憙鍾,金鍾冕,姜明大,蔡孝錫 全北大學校 附設 畜産開發硏究所 1988 畜産開發硏究報告 Vol.2 No.-

In order to enumerate the T lymphocytes in bovine peripheral blood lymphocytes(PBL) by E rosette assay, KGRBC were treated with various concentration of 2-aminoethylisothiouronium bromide hydrobromide(AET) and dextran(Dex), singte or combination. The PBL from 10 clinically normal Korean native cattle were formed as low percentage of spontaneous E rosette(6.7±2.4%) in control group, whereas KGRBC treated with 0.1M AET for 20 minutes and 8%Dex were formed as 37.3±2.7% and 45.1±2.1%, respectively. And the synergistic effects were noted no less than 66.5±5.6% when the KGRBC treated with 0.1M AET and 8% Dex subsequently. These results suggest that the KGRBC would be a useful agent for the enumeration of T tymphocytes by E rosette assay in bovine PBI.

움직임 분석을 이용한 물체 추적 기법

정종면,주영현,문영식 漢陽大學校 工學技術硏究所 1998 工學技術論文集 Vol.7 No.1

본 논문에서는 연속적으로 입력되는 영상의 시공간 정보에서 추출한 특징값들을 이용하여 실시간 물체 추적을 위한 이동 물체 추출 및 얼굴 영역 추출 알고리즘을 제안한다. 제안된 방법은 프레임 분류 관계와 이동 물체 추출 및 얼굴영역 분리 단계의 두 단계로 이루어져 있다. 프레임 분류 단계에서는 모든 입력 프레임에 대하여 시간에 따른 화소값의 변화를 기반으로 하여 세 종류의 특징값들을 추출한 다음, 그 값들을 이용하여 입력 프레임들을 다섯 종류로 분류한다 이 중에서 이동 물체를 포함한 영상으로 분류된 프레임에 대해서는 얼굴 영역 분리 단계에서 컬러 정보와 프레임간의 차이를 이용하여 그 얼굴 영역을 추출 및 추적하게 된다. 제안된 알고리즘을 여러 테스트 영상 시퀀스에 대하여 실험한 결과 유용성이 입증되었다. 제안된 알고리즘은 차영상에 기반하여 시공간 정보를 취득함으로써 계산량이 적어 실시간 물체 추적에 사용될 수 있다. In this paper, a real time moving object tracking algorithm using spatio-temporal information of image sequences is presented. The proposed algorithm consists of two stages : frame classification stage and face localization stage. In the frame classification stage, each frame is classified into one of 5 prespecified classes, using three measures which are based on image pixel difference. If the current frame contains moving objects, their skin regions are extracted using color and frame difference information in the face localization stage. Experimental results tested on various image sequences have shown the robustness of the proposed algorithm. Since the algorithm uses computationally efficient difference images, it is suitable for real-time moving object tracking.

젖산균의 生物學的 特性에 관한 硏究 : Ⅰ. 전산杆菌의 藥劑耐性과 傳達性 R 因子의 分布 Ⅰ. Drug resistance and distribution of transferabel R factors in Lactoacillus

金鍾冕,宋熹鍾 全北大學校 1979 論文集 Vol.21 No.-

Recent reports confirm that R factors against Enterobacteriaceae isolated from various sources in widespread in Korea. However, no report have been made concerning the incidence of transferable R facters of Lactobacillus contained in raw and fermented milk. A total of 80 strains of Lactobacillus isolated from raw and fermented milk of 5 companies were examined to investigate the minimum inhibition concentration(MIC) of the 7 drugs such as streptomycin(SM), nalidixic acid(NA), chloramphenicol(CP), tetracycline(TC), penicillin(PC), kanamycin(KM), and gentamicin(GM). After that they were examined for distribution of transferable R factors. The following conclutions were obtained. Of 69 isolates from raw milk, 51 strains were classified L. cellobiosus, 13 strains as L. plantarum, and 5 strains as others by biological test. Whereas of 11 isolates from fermented milk, 4 strains were classified as L. helveticus, 3 strains as L. bulgaricus, 1 strain as L. casei, and 3 strains as others by the above same methods. The MIC of KM, GM, and PC to the isolates was 10 ㎍/㎖, whereas 100%(80 strains), 98.8% (79 strains), 12.5%(10 strains) and 2.5%(2 strains) of the isolates was resistant to SM, NA, CP, and TC in disk sensitive potency, respectively. Among 80 drug resistant strains of isolates, the rate of double drug resistant patterns (86.2%) were higher than that of triple drug resistant patterns(13.8%) and 4 different drug resistant patterns were observed. The most frequently encountered patterns were SM NA (84.2%), followed by SM NA CP(12.5%), SM TC and SM NA TC(2.5%). Of 69 isolates from raw milk, 4 strains carried R factors to E. coli drug free receipient determining R(SM), 3 strains to E. coli SK-12 receipient determining R(CP). Whereas 11 isolates from fermented milk, 7 strains carried R factors to E. coli drug free receipient determining R(SM) and R(SM TC), 7 strains carried R factors to E. coli SK-12 receipient determining R(CP), R(TC), and R(SM). It might be summerized that Lactobacillus isolated from raw and fermented milk represented that carried R factors between different Families -Lactobacillaceae to Enterobacteriaceae- in bacteria determining from genus Lactobacillus to genus Escherichia.

生乳에서 分離한 大腸菌群의 藥濟耐性 및 R Plasmids

金鍾冕,宋熹鐘 全北大學校 1978 論文集 Vol.20 No.-

Recent reports confirm that R factors against Enterobacteriaceae isolated from various sources is widespread in Korea. However, few reports have been made concerning the incidence of transferable drug resistance of bacteria contained in milk. A total of 114 strains of coliform bacteria were isolated from raw milk, and classified 32 strains as Escherichia coli, 18 strains as Citrobacter freundii, 64 strains as Klebsiella-Enterobacter group by IMViC system. After that, they were examined for drug resistance and distribution of R plasmids. Among 32 strains of E. coli isolated, 78.1% (25 strains) were resistance to antibacterial agents such as streptomycin(SM), chloramphenicol(CP), gentamicin(GM), ampicillin(AP), kanamycin(KM), tetracycline(TC), and nalidixic acid(NA), singly or in combination. Whereas all strains of Citrobacter freundii and Klebsiella-Enterobacter group were resistant to one or more drugs used. Among 25 resistant strains of E. coli isolated, the rate of multiple resistant strains(60%) was higher than that of singly resistant strains (40%) and 15 different drug resistant patterns were observed. The most frequently encountered patterns were AP, followed by SM and AP KM. Among 18 resistant strains of Citrobacter freundii isolated, the rate of multiple resistant strains(66.7%) was higher than that of singly resistant strains(33.3%) and 5 different drug resistant patterns were observed. Meanwhile, the most frequently encountered patterns were NA, followed by SM CP AP and SM CP AP TC. Among 64 resistant strains of Klebsiella-Enterobacter group isolated, the rate of multiple resistant strains(71.9%) was higher than that of singly resistant strains(28.1%) and 21 different drug resistant patterns were encountered. Among the 18 singly resistant strains, AP pattern (11 strains) was the commonest. While among the 46 multiple resistant strains, SM AP TC, SM AP, SM AP KM TC, and SM CP AP KM TC patterns were frequently observed. Each one strain of E. coli and Citrobacter freundii carried R plasmids, determining AP, and CP TC, respectively. Whereas 8 strains of Klebsiella-Enterobacter group carried R plasmids determining TC, AP, CP, CP TC, CP AT TC, and SM AP TC. The transfer frequency rate of AP in E. coli was 4.2×10 exp (-4)%. In Citrobacter freundii the transfer frequency rate of CP was 7×10 exp (-5)%, and that of TC was 5×10 exp (-5)%. In Klebsiella-Enterobacter group the transfer frequency rate of SM was 2×10 exp (-5)%, that of CP ranged from 1.1×10 exp (-4) to 6.7×10 exp (-4)%, that of AP 2×10 exp (-5) to 1.6×10 exp (-4)%, and that of TC 2×10 exp (-5) to 1.02×10 exp (-3)%.

冠岳樹木圓地域內 荒廢山地土壤의 肥沃化를 통한 綠化促進에 關한 硏究(II)

禹保命,權台鎬,李宗學,金景河,李峻雨,麻鎬燮 서울大學校 農科大學 1986 서울대농학연구지 Vol.11 No.2

The shallow soil depth and severe rock exposures on the hillslopes in the Kwanak aboretum area which resulted from the heavy soil erosion have made very poor growth of forest vegetation. In order to establish the forest vegetation in this area, it is urgent to recover the fertility of soil and the productivity of existing trees. For this reason, fertilization experiment (using the briquet compound fertilizer) was conducted on summer and autumn season in 1983 with the 4 main native species (Pinus densiflora, Pinus rigida, Juniperus rigida, Rhododendron schlippenbachii) growing on these eroded hillslopes. Result in 1985 and 1986 of this experiment represented that the fertilization was effective toward both height and root-collar diameter growth of trees and spring fertilization was, relatively, more effective than autumn fertilization. It also represented that effects of fertilization to P. rigida were more than those to other species. Therefore, besides the engineering methods and afforestation measures for soil erosion control, rapid establishment of vegetation through conservation and recovery of existing trees by fertilization is available measures for the rehabilitation of rockily eroded hillslopes like Mt. Kwanak area.

病原性葡萄球菌의 同定을 위한 Coagulase, Deoxyribonuclease(DNase) 및 耐熱成 nuclease 生産能의 比較

金鍾冕 全北大學校 生物學硏究所 1981 生物學硏究年報 Vol.2 No.-

A total of 251 clinical isolates (human origin 43 strains and bovine udder origin 208 strains) of the Staphylococcus that fermented mannitol aerobically were tasted for their ability to produce coagulase, deoxyribonuclease(DNase), and thermostable nuclease. Of these, 158 isolates coagulated human or bovine plasma, produced DNase and thermostable nuclease, and were identified as St. aureus, 146 which produced a 1+ to 3+ clot. The remaining twelve isolates produced a ± or - clot with citrate treated plasma but produced 1+ to 3+ clot with ethylenediaminetetraacetic acid(EDTA) treated plasma. It was found that seven coagulase positive isolates failed to produced thermostable nuclease. In these organisms, we found out of the clot formation is not by coagulase activity but utilization of citrate, because EDTA treated plasma is not coagulated. Among 93 isolates which did not coagulate citrate-or EDTA treated plasma and thermostable nuclease negative, 28 strains produced DNase were identified as St. epidermidis, and other strains were not identification further. It was found that thermostable nuclease production appears to be a consistent property of St. aureus and the test is easy to perform, is rapid - became quite within 2 to t hour, and is not influenced by as many factors and variations as the coagulase test.

發癌劑 3-Methylcholanthrene 投與 마우스에 對한 免疫生物學的 硏究 : Ⅰ. 足蹠腫脹反應 및 血中抗體價 I. Footpad Swellig Reaction and Antibody Titer in Serum

宋熹鍾,金鍾冕 全北大學校 附設 畜産開發硏究所 1987 畜産開發硏究報告 Vol.1 No.-

Experiments were performed on mice to investigate the effects of a polycycic aromatic hydrocatbon, 3-methylcholanthrene (MCA) on Arthus reaction, delayed-type hypersensitivity (DTH) and antibody production to sheep red blood cells (SRBC). Mice were sensitized iv with 0.1㎖ of 1% SRBC suspension were treated with a single ip injection of olive oil alone or with different doses of MCA in oil (0.5∼50㎎/㎏0 at various time before (-) or after (+) sensitization (day 0) and were challenged at 4 days after SRBC. Atrhus reaction was measured at 3 hours after challenge and other responses at 24 hours. Treatment with MCA inhibited Arthus reaction and DTH to SRBC, measured by footpad swelling reaction, and this immunosuppressing effect was dependent on the dose and time of MCA treatment in relation to SRBC sensitization. Humoral immune responses as measured by serum hemagglutinin-and hemolysin-titers to SRBC were significantly depressed when MCA was injected before or at the same time of sensitization. However, the response was slightly depressed when injected after SRBC. These result indicate that MCA suppress the function of the cells involved in immune responses.

두류산(頭流山)

나종면 숲과 문화 연구회 2004 숲과 문화 Vol.13 No.5

人蔘이 細菌의 藥劑耐性 및 耐性因子의 傳達에 미치는 影響

金鍾冕 全北大學校 1984 論文集 Vol.26 No.-

In order to evaluate the possible role of Korean Panax ginseng on the variation of the bacterial characteristics against antibiotics, each strain of E. coli subcultured on the media containing ginseng-alcohol extract (1㎎/㎖) and the media without the extracts, was examined for drug resistance, transferability and transfer frequency of R plasmids in the presence or absence of ginseng extract (1㎎/㎖). Some differences in antibiotic susceptibility were found between strains by the condition of subcultured media or susceptibility test media. No prticular effect of ginseng extract was observed on R plasmids transferred, but the transfer frequency of R plasmids was slightly increased when ginseng extract was added on the test media. These results indicate that ginseng extract mayy alter some chracteristics of bacteria.

湖水의 環境保全에 있어서의 生物化學的 硏究 : 玉井湖의 營養鹽과 Vitamin B_12의 含量 Distribution of Chemical Compounds and Vitamin B_12 of Okjeong Lake

金鍾冕,朴忠雄,田銀順,孔錫宗 全北大學校 附設 畜産開發硏究所 1987 畜産開發硏究報告 Vol.1 No.-

Concentration of dissolved vitamin B_12 in the water of Okjeong lake have been determined by microbiological assay methods on the 20th of August, 1983. Also the relations between the vitamin B_12 and other physical, chemical and biological environmental factors were investigated. Vitamin B_12 was assayed with Euglena gracilis Z strain. The results obtained in this investigation were as folows:1. Generally, the values of COD and Cl^- were higher than values reported in 1981. 2. The concentration of nutrient salts in the lake water was found to be at a higher level: above the general criterion in a eutrophic lake viz., phosphorous 0.175-0.225 ㎎/l. 3. Chlorophyll a contents in this lake were 1.1-8.7 ㎍/l. 4. The concentration of vitamin B_12 in the water fairly high: 1.25-6.0 ng. It is apparent that concentrations of the vitamin B_12 as well as nutrient salts were high in the water of Okjeong lake. It was found that Okjeung lake was in eutrophic.