C3H/Hej 마우스에서 모발성장에 대한 생약 추출물의 효과

구성근, 주진호, 허진주, 미가, 강봉수, 김준형, 남상윤, 윤영원, 이범준, 김종수 충북대학교 동물의학연구소 2008 Journal of Biomedical and Translational Research Vol.9 No.3

본문참고

Biomass 자원의 활용 (Ⅰ) : 율추의 유효이용을 위한 화학적 조성분의 HPLC 분석 HPLC Analysis of Chemical Components for Utilization of Chestnut Inner Bark

金允根,趙種洙 한국목재공학회 2004 목재공학 Vol.32 No.2

임산바이오매스 자원 이용의 일환으로 율추(栗皺) 열수추출물의 디에틸에테르 가용부의 HPLC 분석결과, 표준물질과의 retention time이 일치한 것을 RI detector로 얻어진 각 피크의 스펙트럼을 표준물질과 비교하고, 순도를 확인하는 방법으로 피크의 동정을 실시하였다 피크의 동정 결과, phenolic acids와 aldehyde류로는 gallic acid, 3.5-dihydroxybenzoic acid, 2.4.6-trihyd개rxybenzoic acid. 그리고 protocatechualdehyde이었으며, flavonoids는 catechin과 epicatechin으로 모두 6종이 확인되었다. For the utilization of chestnur inner bark as forest biomass, the diethyl ether solubles of hot water extract from chestnut inner bark was analyzed by HPLC. Each peak was identified by comparing with retention time of standard regents and their purity from obtained UV speccrum by RI detector. Identified 6 compounds were gallic acid 3,5-dihydroxybenzoic acid, 2,4,6-trihydroxybenzoic acid and protocatecualdehyde as phenolic acids and aldehyde, and catechin and cpicatechin as flavonoids.

임산유지수종의 이용에 관한 연구 (Ⅴ) : 장기저장에 따른 때죽나무 종실의 성분 변화 Variation of Components in Sapium japonica Seed Following Long Terms Storge

조종수,강병국,양재경,박종열,김윤근 진주산업대학교 농업기술연구소 2000 農業技術硏究所報 Vol.13 No.-

This paper was carried out to analyze the variation of composition of seed oil with storage periods. Difference of content of crude fat in fresh-cut seed and one year storage seed is not detected but in pentadecanoic acid, acid value, iodine value and peroxide value in one year storage seed have lower than those of the fresh-cut seed. Linoleic acid and oleic acid were high among of total fatty acid in fresh-cut seed but moiety of linolenic acid and icosanoic acid was low otherwise that of palmitic acid and oleic acid is increased in one year storge. In neutral lipid, capric and mytistic acid were high in fresh-cut, oleic acid, palmitic acid and linoleic acid were increased after one year storge. In glycolipid, the lauric acid and linoleic acid were high in fresh-cut seed, the palmitic acid was increased, otherwise, linolenic acid was decreased after one year storge. In phospholipid, palmitic acid and unknown part were high in fresh-cut seed, palmitic acid, oleic acid and linolenic acid were increased after one year storge.

생활치 미백제가 우치 법랑질의 투명도에 미치는 영향

김동준,김영주,김학근,박은종,서은주,황윤찬,오원만,황인남 大韓齒科保存學會 2005 Restorative Dentistry & Endodontics Vol.30 No.3

본 연구에서는 현재 임상에서 home bleaching을 위해 사용되고 있는 표백 재료들이 치아 법랑질의 투명도에 어떠한 변화를 일으키는지 고찰해 보고자 시행하였다. 실험 대상으로는 하루 전 발거한 우치 (bovine tooth)를 사용하였다. 치아의 순면에 수직되게 다이아몬드 드릴을 이용해 수주 하에서 직경 6 mm의 원통형 치아 시편을 만들어 순면이 노출된 상태로 투명한 아크릭 레진에 포매한 후, low speed diamond saw (Isomat, Buehler Co., USA)를 사용하여 1.2 mm 두께로 절단하였다. 절단된 시편은 순면을 먼저 편평하게 연마하고,절단된 면을 연마하여 두께 1 mm의 시편을 완성하였다. 완성된 시편(우치 25개)을 무작위로 추출하여 3개의 그룹으로 나누고 나머지는 대조군으로 하였다. Opalescence (10% carbamide peroxide, Ultradent, Milford, USA), Rembrandt (10% carbamide peroxide, DenMat, USA) 및 Opalescence F(15% carbamide peroxide with fluoride, Ultradent)를 시편의 순면에 1주일 동안 적용 (표백제는 24시간마다 교체)하였고, 설측면은 증류수를 적신 거즈에 접촉된 상태로 보관하였다 표백제를 적용하기 전과 적용 3, 5, 7일째에 시편의 삼자극치 X, Y, Z 값을 색채색차계 (Color & Color Differencemeter, Model TC-6FX, Tokyo Denshoku Co., Japan)로 백색 배경판과 흑색 배경판 상에서 측정하였다 백색 배경판과 흑색 배경판 상에서 측정된 Y값의 평균값을 이용해 contrast ratio를 계산하였다 얻어진 값을 One Way Repeated Measured ANOVA를 이용하여 통계 처리한 결과 10% Opalescence처치군, 15% Opalescence-F처치군 및 대조군에서는 통계적으로 유의할만한 증감을 보이지 않았고, Rembrandt처치군에서는 Contrast ratio가 증가하였다 (p < 0.01) . This study was done to evaluate whether vital bleaching agents could influence on the translucency of the bovine enamel. The anterior bovine teeth that were extracted one day before and without any gross discoloration were obtained and then were preserved in physiologic saline. 6 mm cylindrical tooth specimens were fabricated with diamond puncher perpendicularly on labial surface of bovine tooth. After embedded in transparent acrylic resin with labial surface being exposed, they were cut to a thickness of 1.2 mm with low speed diamond saw (Isomat, Buehler Co., Lake Bluff, IL, USA). They were smoothly ground to 1 mm thickness of enamel with sandpaper. 24 specimens were randomly divided into 3 groups and control group respectively. Opalescence (10% carbamide peroxide, Ultradent, South Jordan, USA), Rembrandt (10% carbamide peroxide, DenMat, USA) and Opalescence F (15% carbamide peroxide with fluoride, Ultradent, USA) were applied on labial sides of the bovine enamel for 7 days (bleaching agents were reapplied every 24 hours) and the opposite surface was contacted to cotton that soaked in distilled water. The control group was soaked in distilled water. Three stimulus value X, Y and Z were evaluated with colorimeter (Color ' Color Differencerneter, Model TC-6FX, Tokyo Denshoku Co., Japan) on the labial surface of all specimen three times on white and black background plate before the bleaching agents were applied and on 3rd, 5th and 7th day after applied. The degree of translucency was normally assessed by measuring the inverse property, opacity (contrast ratio). 10% Opalescence, 15% Opalescence-F, and control group showed no significant variation in the translucency of bovine enamel, However Rembrandt decreased the translucency of it (p < 0.01).

Floristic Study and Conservation Plan on Abandoned Paddy Field Type of Forest Wetlands in Korea

Jong-Won Lee(Jong-Won Lee),Ho-Geun Yun(Ho-Geun Yun),Jong-Bin An(Jong-Bin An) 한국자원식물학회 2022 한국자원식물학회지 Vol.35 No.6

This study surveyed the vascular flora present in abandoned paddy field type of 237 forest wetlands in Korea to provide baseline data for conservation, management, and monitoring and to support preparation for damage and climate change. The study period was from April 2015 to November 2021. The vascular flora of 237 forest wetlands by abandoned paddy field type were identified to consist of 128 families, 510 genera, 1,022 species, 27 subspecies, 87 varieties, 10 forma, and 1,146 taxa. 4 taxa were identified as grade II endangered wild plants, 185 taxa were identified as northern lineage plants of the Korean Peninsula, 43 taxa were endemic to the Korean Peninsula, and 58 taxa were rare plants as designated by the Korea Forest Service. Further, 296 taxa were floristic target plants and there were 86 taxa of invasive alien plants. According to wetland preference, 79 taxa (6.9%) were obligate wetland plants, 103 taxa (9.0%) were facultative wetland plants, and 61 (5.3%) taxa were facultative plants. Additionally, 66 taxa (5.8%) were facultative upland plants, and 837 taxa (73.0%) were obligate upland plants.

119지역대의 공간적 배치 현황의 적절성

윤종근(Jong-Geun Yun),최규철(Kyu-Chul Choi) 한국산학기술학회 2017 한국산학기술학회논문지 Vol.18 No.11

본 연구는 시골지역에 위치한 119지구대의 현황을 분석하고 이를 바탕으로 좀 더 효율적인 응급의료서비스를 이용할 수 있도록 공간적 배치의 기초자료를 제공하기 위해 Y시 H면에 소재한 119지구대를 중심으로 2016년1월 1일부터 2016년12월 31일까지의 119지역대 환자이송 현황을 조사하였다. 수집된 자료는 SPSS 21.0을 이용하여 분석하였으며, 빈도분석을 실시하고, 가상적인 119지구대 배치 후 이송시간 및 거리는 GIS(Geographic Information System) 를 이용하여 정보를 분석하였다. 연구결과, 119지구대를 이용율은 대부분 65세가 넘은 고령환자가 전체 이용환자의 57%인 수준이었으며, 실제 출동반응 현황은 현장도착 평균 거리는 6.41km(11.86분)이었으며, 현장에서 의료기관까지 소요되는 거리는 18.24km(21.52분)었다. 현재 위치하고 있는 119지역대를 중심으로 GIS를 이용하여 분석한 결과 장*리 마을로 9.12km(12분)이 소요되었으며, 환자발생지역까지 출동 후 다시 의료기관까지 가는 거리는 36.83km(62분)이 소요되는 것으로 나타났다. 장*리로 가상지구대를 설정한 경우 의료기관까지이 거리는 27.71km(50분)정도 소요되는 것으로 나타났다. 따라서 본 연구의 결과를 분석에 의하면 현재 소재하고 있는 119지역대의 위치는 환자발생지점에서 의료기관까지의 거리를 감안해 볼 때 적절하지 않은 것으로 나타났다. 이에 좀 더 많은 지역의 지구대를 중심으로 활발한 연구를 통하여 환자발생시 119지구대가 출동 시 최단의 거리와 환자 발생 지점에서 의료기관까지의 최단 거리등을 고려하여 현재 설치되어 있는 119지구대의 위치를 재고해야 될 것이다. In this study, we analyzed the situation of the 119 emergency medical service zone of H town, countryside of Y city from January 1st, 2015 to December 31st, 2016 and then, on the basis of this analysis, we investigated the present condition of the patient-transportation service of the 119 emergency medical service to provide the basic data in order for patients to use the emergency medical service more efficiently. We analyzed the data with SPSS 21.0 using frequency analysis and, after positioning the virtual 119 emergency medical service, we analyzed the data of the transportation time and transportation distance by using GIS. The results of this study show that the use of the 119 emergency medical service for people over 65 years old represents approximately 57% of the total number of patients transported, The average distance and time of the real moving reaction are 6.41 km and 11.86 min, respectively. The distance and time from the pick-up location to the hospital are 18.24 km and 21.52 min, respectively. Given the present position of the 119 emergency medical service, the results of this analysis using GPS show that the (average) distance and time from the 119 emergency medical service to Jang * Ri town are 9.12 km and 12 min, and the (average) total distance and time to arrive at the hospital after the emergency medical service picks up the patient are 36.83 km and 62 min, respectively. In the case of the virtual emergency medical service, the total distance and time required to arrive at the hospital after the emergency medical service picks up the patient are 27.71km and 50min, respectively. The results of this study showed that the present position of the 119 emergency service does not provide the optimum distance and time from the patient"s location to the hospital. Therefore, we consider that the repositioning of the 119 emergency medical center is necessary, in order to reduce the time required for the emergency medical service to move to the patient"s location and then bring the patient to the hospital.

응급구조사에 대한 이미지와 업무인식 조사

윤종근(Yun Jong-Geun),정영(Chung Young),박미영(Park Mi-Young) 조선대학교 지식경영연구원 2004 지역개발연구 Vol.9 No.2

scEmergency Medical Technicians act not only as rescue-workers at the ene but also in charge of many supporting roles for the medical staff at the hospitals. However it is true that there is not a lot of awareness about the EMTs in the hospital. In order to find out the image of EMTs. descriptive research was carried out on 156 nurses who make up a great part of the medical staff with the goal of developing the profession of EMTs as well as to provide the basic information to develop a strategy for enhancing the image of EMTs. The results of this study were as follows: 1. The credit for image for EMTs of the subjects was 3.25, the most high credit was 3.58 for the 'job', and most low credit was 2.83 for the 'role' 2. The credit for the 'job perception' was 0.44. as sub-scope. 0.78 for the 'hospital stage', 0.10 credit for 'medication'. 3. There was no statistically significant difference on image among general characteristics of the subjects. 4. There was no statistically significant difference on job-perception among general characteristics of the subjects. 5. There were no statistically significant difference on image among the job related characteristics of the subjects. 6. There were no statistically significant difference for the job-perception among the job related characteristics of the subjects.

문수산(김포) 산림유전자원보호구역 관속식물상 변화 및 관리방안

윤호근(Ho Geun Yun),이아영(Ah young Lee),안종빈(Jong Bin An),황태영(Tae Young Hwang),이종원(Jong Won Lee) 한국자원식물학회 2021 한국자원식물학회지 Vol.34 No.4

본 연구는 경기도 김포의 최서북단 DMZ 접경지역 및 민북지역에 위치한 문수산의 산림유전자원보호구역 일대의 관속식물상과 침입외래식물 등을 파악하고 확인된 식물들의 효율적인관리와 생물다양성 보전을 위한 기초자료로 활용하기 위하여실시하였다. 조사는 2019년 4월부터 2020년 10월까지 총 17회에 걸쳐 실시되었다. 문수산 산림유전자원보호구역 일대의 관속식물상은 총 95과 276속 395종 13아종 33변종 3품종 444분류군이 분포하는 것으로 확인되었다. 이는 우리나라 관속식물 총4,881분류군의 약 9.09%로 나타났다. 문수산 산림유전자원보호구역에서 출현한 한반도 특산식물은 은사시나무, 오동나무, 산이스라지, 백운산운추리 등 6종이 관찰되었다. IUCN 지정 희귀식물은 총 3분류군이 관찰되었다. 위기종(EN)은 복사앵도나무, 약관심종은 쥐방울덩굴과 창포가 출현하였다. 식물구계학적 특정식물은 초 39분류군으로 파악되었다. IV등급은 바위말발도리 등 2분류군, III등급은 고광나무, 병아리꽃나무 등 8분류군이 확인되었다. II등급은 잣나무 및 오리나무 등 총 10분류군이 관찰되었다. 조사지역에서 출현한 침입외래식물은 개망초 , 달맞이꽃 및 개쑥갓 등 58분류군으로 확인되었다. 귀화율은 13.1%로 나타났으며, 도시화지수는 18.0%로 계산되었다. 본연구에서 파악된 문수산 산림유전자원보호구역의 관속식물상과 선행연구와 비교한 결과, 최근 조사 구간이 더 넓고 다양한경로를 조사했음에도 불구하고 선행연구에 비해 분류군 수가감소한 것으로 파악되었다. 특히 희귀·특산식물의 수는 크게감소하고 외래침입식물의 수는 임도 및 등산로 등으로 크게 확산한 것으로 확인되었다. 따라서 산림유전자원보호구역 내 자생식물의 보전 및 관리를 위해 산림휴식년제 등을 도입해야 할것으로 생각된다. This study was investigated to find out the distribution of vascular flora and remarkable plants and its factors and management plan in the forest genetic resource reserve of Mt. Munsu, located in the DMZ and DMZ border area in Gimpo, Gyeonggi-do province. The survey was carried out 17 times from April 2019 to October 2020. First of all, in the forest genetic resource reserve of the Mt. Munsu, a total of 444 taxa in 95 families, 276 genera, 395 species, 13 subspecies, 33 varieties and 3 forms. This result was found to be about 9.09% of the total 4,881 taxa of vascular plants in Korea. In addition, endemic plants were classified as 6 taxa. Floristic special and rare plants were identified as 39 taxa and 3 taxa, respectively. Lastly, the invasive alien plants that appeared in Mt. Munsu of the forest genetic resource reserve were observed in 58 taxa. Moreover, the naturalization rate was 13.1% and the urbanization index was calculated to be 18.0%. As a result of comparing the vascular flora at the forest genetic resource reserve on Mt. Munsu identified in this study with previous studies, it was found the number of taxa was decreased compared to the previous studies, despite the fact that the recent survey section was wider and more varies routes were investigated. In particular, it was confirmed that the number of rare and endemic plants decreased significantly, and the number of invasive plants greatly spread to forest roads and hiking trails. Therefore, it is considered that the forest rest year system should be introduced for the conservation and management of native plants in the forest genetic resource reserve.

NBR과 MaxEnt 모델 분석을 활용한 희귀특산식물(개느삼) 분포 및 피해량 예측 : 양구 비봉산 산불피해지를 대상으로

윤호근(Ho-Geun Yun),이종원(Jong-Won Lee),안종빈(Jong-Bin An),유승봉(Seung-Bong Yu),박기쁨(Gi-Ppeum Bak),신현탁(Hyun-Tak Shin),박완근(Wan-Geun Park),김상준(Sang-Jun Kim) 한국자원식물학회 2022 한국자원식물학회지 Vol.35 No.2

This study was conducted to predict the distribution of rare·endemic plants (Sophora koreensis Nakai) in the border forests where wildfire damage occurred and to quantify the damage. For this purpose, we tried to derive more accurate results through forest area damage (NBR) according to the Burn severity of wildfires, damage by tree species type (Vegetation map), and MaxEnt model. For Burn severity analysis, satellite imagery (Landsat-8) was used to analyze Burn severity (ΔNBR2016-2015) and to derive the extent of damage. To prepare the Vegetation map, the land cover map prepared by the Ministry of Environment, the Vegetation map prepared by the Korea Forest Service, and the vegetation survey conducted by itself were conducted to prepare the clinical map before and after the forest fire. Lastly, for MaxEnt model analysis, the AUC value was derived by using the habitat coordinates of Sophora koreensis Nakai based on the related literature and self-report data. As a result of combining the Maxent model analysis data with the Burn severity data, it was confirmed that 45.9% of the 44,760 ㎡ of habitat (predicted) area of Sophora koreensis Nakai in the wildfire damaged area or 20,552 ㎡, was damaged.

갈근 및 녹차추출물의 아질산염 소거작용 및 간독성 보호효과

윤이란 ( I Ran Yun ),최유정 ( You Jeong Choi ),허정호 ( Jung Ho Heo ),최철웅 ( Chul Yung Choi ),성태종 ( Tae Jong Seoung ),김윤근 ( Yun Geun Kim ),김종수 ( Jong Shu Kim ) 한국가축위생학회 2010 韓國家畜衛生學會誌 Vol.33 No.3

This study was designed to evaluated the nitrite scavenging activity and protective effect of the Puerariae Radix and green tea extract on lead acetate and cadmium-induced liver damage in mice. The quantitative analytical method for major antioxidants, isoflavones, puerarin, catechine and caffeine in galgun (Puerariae Radix) and green tera extract were established by HPLC. Contents of isoflavones, such as daidzin, genistin, daidzein and genistein were 4.23g/100g, 0.13g/100g, 0.07g/100g, and 0.03g/100g, and puerarin contents was 8.99g/100g, respectively. The total catechins and caffeine contents of green tea extract were 49.24g/ 100g and 6.53g/100g. The nitrite scavenging ability of galgun extract (pH 1.2, 100mg/ml) was 98.07% and it was higher than those of other extracts. It was the highest at the pH 1.2 and more than 64% in 25~ 100mg green tea extract, and was dependents on pH and concentration of the samples. The hepatoprotective effects of an aqueous extract from the root of galgun and standard puerarin were evaluated against lead acetate and cadmium-induced liver damage in mice. Galgun extract and standard at a dose of 100mg/kg and 10mg/kg, 50mg/kg were administered orally once daily for successive 5 days and then a lead acetate and cadmium were orally at 3 hrs after the every day administration of galgun. The substantially elevated serum enzymatic activities of alanine and aspartate aminotransferase were due to lead acetate and cadmium treatment was dose dependently restored to the near normal level. In addition, galgun extract also significantly prevented the elevation of hepatic malon-dialdehyde formation in the liver of lead acetate and cadmium intoxicated mice in a dose-dependent manner. The results of this study clearly indicated that green tea and galgun extracts had nitrite scavenging ability and galgun extract had potent hepato-protective effects against lead acetate and cadmium-induced hepatic damage in mice and standard puerarin was also showed similar to the results of the galgun extracts.