Aspergillus 속 미생물에 의한 발효비지의 항비만 효과

이상일(Lee, Sang-Il),이예경(Lee, Ye-Kyung),김순동(Kim, Soon-Dong),임종환(Lim, Jong-Hwan),서주원(Seo, Ju-Won),이인애(Lee, In-Ae) 한국산학기술학회 2013 한국산학기술학회논문지 Vol.14 No.11

고지방식이로 비만을 유도한 ICR 생쥐를 동물 모델로 하여 7종의 Aspergillus속 미생물로 발효시킨 발효비지 의 항비만 효과를 조사하였다. 수컷 ICR 생쥐에 5주간 고지방 식이를 실시하여 비만을 유도하고 고지방식이에 발효 비지 2%를 첨가하여 6주 동안 사육하였으며, 체중, 섭취량, 혈청 총콜레스테롤, 중성지질, Alanine transaminase과 high-density lipoprotein cholesterol, 간성 glutathione과 lipid peroxide를 측정하였다. 체중의 경우에 AE4 발효 비지를 식이한 군에서 가장 많이 감소 (31.33%, P<0.05)하였고 간성 GSH와 ALT증가로 고지방혈증이 저해되었다. 이상의 결 과로부터 Aspergillus속 미생물로 발효시킨 발효비지는 항비만 효과가 있는 것으로 나타났으며, 나아가 인체에서도 유 의한 비만개선 작용을 기대할 수 있을 것으로 사료된다. The anti-obesity effect of soybean curd residues (biji) fermented by seven Aspergillus spp. was investigated with obese ICR mice fed a high-fat diet. After inducing obesity by feeding high-fat diet for 5 weeks, animals were fed with a high fat diet supplemented with 2% fermented soybean curd residues for 6 weeks. Total cholesterol, triglyceride, Alanine transaminase, high-density lipoprotein cholesterol, hepatic content of glutathione and lipid peroxide were determined. In the case of body weight, AE4 group showed most prominent decrease (31.33%, P<0.05) and increase of hepatic GSH and ALT demonstrated hyperlipidemia inhibition. From the results, it is concluded that soybean curd residues fermented by Aspergillus spp. has anti-obesity effect and it is thought that fermented soybean curd resides can reduce obesity in human significantly.

석기암 및 그 인근지역에 분포하는 관속식물상과 주요 식생 현황

양종철, 엄정애, 장창석, 장창기, 이철호, 김규식, 오병운 忠北大學校部設基礎科學硏究所 2006 自然科學硏究 Vol.20 No.-

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백운산(제천시) 일대의 식물상

양종철, 엄정애, 장창석, 장창기, 이철호, 김규식, 오병운 忠北大學校部設基礎科學硏究所 2006 自然科學硏究 Vol.20 No.-

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월악산 국립공원 내 금수산 일대의 식물상

양종철, 엄정애, 장창석, 장창기, 이철호, 김규식, 오병운 忠北大學校部設基礎科學硏究所 2006 自然科學硏究 Vol.20 No.-

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중합효소연쇄반응을 이용한 도말양성 폐결핵 환자에서의 결핵균 DNA 검출방법에 관한 연구

이종진,김애경,조해정,한표성,홍석철,김주옥,김선영 충남대학교 의과대학 지역사회의학연구소 1994 충남의대잡지 Vol.21 No.2

Polymerase chain reaction (PCR) is the most sensitive and rapid method for the diagnosis of M.tuberculosis infection. Many reports have shown different methods for mycobacterial DNA extraction, but revealed many differences in simplicity and time-consumption. We studied which method was the best among 7 different ones for DNA extraction from 32 smear-positive sputa, using PCR of targeting 123bp DNA fragment of IS6110. The methods were the following : SDS-Microwave oven method, NaOH Lysis method, Triton X-100, Proteinase K method, Lysis buffer method. SDS-Proteinase K method, Bead Beater method, and Bead Beater/CTAB method. The amplification products were identified by electrophoresis on ethidium bromide-stained agarose gels, and confirmed by restriction enzyme, Southern blot hybridization, and DNA sequencing. All 32 cases were positive (100%) by the 7 different methods. It took 1 hour or more to detect DNA in SDS-Microwave oven method, NaOH Lysis method, and Bead beater/CTAB method. It took 2 hours or less in the others. SDS-Proteinase K method and Bead Beater/CTAB method were simpler and more convinient than the others. These results suggest that SDS-Proteinase K method and Bead Beater/CTAB method might be better than the others for the extraction of mycobacterial DNA in terms of simplicity and time-consumption, although all the methods were sensitive.

갈근(葛根)으로부터 분리된 puerarin의 항당뇨 효과

임현애,임지선,김정상 경북대학교 농업과학기술연구소 2006 慶北大農學誌 Vol.24 No.-

본 연구에서는 갈근 및 그의 주 이소플라본인 puerarin의 활성을 세포수준에서 분석하였다. 먼저 갈근에 함유된 이소플라본의 양을 분석한 결과, puerarin이 총 이소플라본의 90 % 차지하였다. 다음으로는 puerarin의 항당뇨 활성을 검정한 결과 먼저 탄수화물 및 지방소화효소저해활성에 대해서는 거의 미비한 것으로 나타났으나 인슐린 감수성 및 지방세포의 분화의 유도에 대해서는 농도의존적으로 작용하는 것으로 관찰되었다. 따라서 puerarin은 지방조직내로 포도당의흡수를 촉진함으로서 항당뇨 효능을 발휘하는 것으로 추정된다. In this study we evaluated the anti-diabetic potential of Puerariae Radix and its isoflavone (puerarin) by investigating their inhibitory activities against digestive enzymes, α-amylase, α-glucosidase and lipase and effect on glucose uptake and PPAR γ expression. The activities of carbohydrate digestive enzymes were not inhibited by puerarin. Glucose uptake in differentiated adipocytes was stimulated by puerarin. Furthermore, puerarin enhanced the differentiation of preadipocytes as evaluated by triacylglycerol (TG) accumulation, which is specific for differentiated adipocytes. The effect of puerarin on expression of peroxisome proliferator-activated receptor γ (PPAR-γ) gene, which is associated with obesity and dyslipidemia, was examined by both real-time PCR and reverse transcriptase PCR. The study demonstrated that puerarin increased the expression of PPAR-γ. In conclusion, puerarin showed potential to exert anti-diabetic action by enhancing cellular glucose uptake and thereby TG accumulation in adipocyte tissue.

數學 및 科學 敎育 授業現場에서의 Portfolio의 適用 : 基礎 硏究(Ⅰ) Preliminary Study(Ⅰ)

裵鐘洙,徐熹愛 서울교육대학교 과학교육연구소 1995 科學과 數學敎育 論文集 Vol.21 No.-

The term portfolio has become a popular word in areas of educational research. Unfortunately, the background of educational theories and philosophical perspectives has not been clearly established to support the effectiveness of the use of portfolios in classrooms. In this study, we have reviewed the definitions of portfolio, have analyzed the effectiveness of portfolio in mathematics and science education, and have discussed suggestions and guidelines for teachers how to use portfolios in their classroom. Further, from this study, we proposed that portfolio is grounded in learning process which aligned with Constructivism and individualized instruction. Learning characteristics of students in portfolio classroom include self-refelction upon their own learing, continuous growth in learning, and self-motivated learning. Students also improve academic, personal management, and teamwork skills in the process of learning. Particularly, the use of portfolio in science and mathematics classrooms enhance student's problem solving skill, creativity, reasoning skill, scientific thinking skill, and ability to apply knowledge to real world. It was recommended that teachers and teacher educators need to understand the nature of portfolio in order to effectively implement portfolios in classrooms. Changes in current classroom culture toward portfolio approach would stimulate more effective student learning and improve science and mathematics education in the country.

무용전공학생의 호흡순환기능 및 혈중젖산농도에 관한 비교연구

이종희,김말애,손흥기,김민정 경희대학교 체육과학연구소 1996 體育學論文集 Vol.24 No.-

The purpose of this study is to investigate the cardiovascular function and blood lactate in female by part of dance major in university. For this purpose a total of 32 female students, attending university K in Seoul participated in the study. The subjects were selected randomly 24 female students majored in dance in university(Korean dance: 8, Modern dance: 8, Ballet: 8), and 8 regular female students, who did not have any dance experience. The results of the study were as follows; 1.Cardiovascular function 1)In factors of rest HR showed in Control group 76.50±3.74 beats/min, Korean dance group 72.13±4.85 beats/min, Modern dance group 71.50±4.93 beats/min, Ballet dance group 68.8±4.19beats/min. Among these group there was significantly(p〈.05). 2)In factors of HRmax showed in Korean dance group 200.63土4.78 beats/min, , Ballet dance group 198.00±6.28beats/min, Modern dance group 197.63±6.65beats/min Control group 189.75±10.51beats/min. Among these group there was significantly(p〈.05). 3)In factors of VO2/kg showed in Ballet dance group 44.46±4.09㎖/min, Korean dance group 43.95±2.95㎖/min, Modern dance group 40.73±3.26㎖/min, Control group 34.33±2.28㎖/min. Among these group there was significantly(p〈.001). 4)In factors of VCO2 showed in Korean dance group 84.31ℓ/min, Modern dance group 2.60±0.44ℓ/min, Ballet dance group 2.52±0.23ℓ/min, Control group 2.23±0.34ℓ/min. Among these group there was not significantly. 5)In factors of art-out time showed in Korean dance group 931±52.92sec, Modern dance group 916.75±40.96sec, Ballet dance group 908.88±28.44sec, Control group 809.38±70.02sec. Among these group there was significantly(p〈.001). 2.Blood lactate 1)In factors of rest time blood lactate showed in Ballet dance group 1.17±0.26.mM/ℓ, Control group 1.11±0.21mM/ℓ , Korean dance group 1.09±0.28mM/ℓ , Modern dance group 1.06±0.33mM/ℓ Among these group there was not significantly. 2)In factors of all-out time blood lactate showed in Control group 9.14±0.92mM/ℓ, Ballet dance Broup 7.79±1.14mM/ℓ, Korean dance group 7.67±1.51mM/ℓ, Modern dance group 7.42±1.18mM/ℓ, Among these group there was not significantly. 3)In factors of recovery 3 minute blood lactate showed in Control group 9.52±0.94mM/ℓ, Ballet dance group 7.68±1.73mM/ℓ, Modern dance group 7,61±0.89mM/ℓ, Korean dance group 7.52±1.78mM/ℓ. Among these group there was significantly(p〈.05). 4) In factors of recovery 5 minute blood lactate showed in Control group 9.91±1.22mM/ℓ, Korean dance group 7.97±2.38mM/ℓ, Ballet dance group 7.86±1.86mM/ℓ, Modern dance group 7.51±1.10mM/ℓ, Among these group there was significantly(p〈.05). 5)In factors of recovery 10 minute Control group 8.99±0.97mM/ℓ, Ballet dance group 6.98±1.65mM/ℓ, Korean dance group 6.96±2.08mM/ℓ, Modern dance group 6.78±1.38mM/ℓ, Among these group there was significantly(p〈.05).

3차원 인체 스캔 데이터를 활용한 남성용 바디스 원형 설계 방법 연구

서동애,천종숙 服飾文化學會 2004 服飾文化硏究 Vol.12 No.2

The purpose of this study is to testify the pattern making method to develop the men's basic bodice pattern using 3-dimensional body scan data. The experimental patterns were made by adding wearing ease on flattened body scan data and tracing the outlines of it. The experimental bodice pattern were composed of front, back, and side panels. To compare the difference between the experimental pattern and traditional pattern, two pattern making methods were compared. Two sets of basic bodice patterns were made for each of the 10 male subjects: a set of pattern was made by experimental method and the other set was made by Bunka pattern making method. The experimental and traditional patterns were measured at 13 dimensions. The results show that there was a difference between the experimental patterns and traditional patterns at the front length, back length, front width, front neck width, back neck width, and back neck depth. The fit was also compared for both patterns. The results of the fit test show that the experimental patterns were superior to the traditional patterns at the fit of neck, shoulder, and armhole. The experimental pattern making method was expected to be useful for mass customization.

입원 중인 베트남 참전 군인의 삶의 만족도와 관련된 요인

김애리,박종,김율,노희송,박광희,김은숙,김형철,홍강식,류소연,강명근,김기순 朝鮮大學校 附設 醫學硏究所 2007 The Medical Journal of Chosun University Vol.32 No.2

Background and Objectives: The aim of the study was to identify the factors associated with life satisfaction of hospitalized Vietnam veterans. Material and Methods: The data were collected from 200 hospitalized Vietnam veterans of 5 veterans hospitals in Korea from September 13 to 23, 2004. Information of general characteristics, health behavior, disease history, family function, depression and life satisfaction were collected by structured questionnaire. Results: The mean of life satisfaction was 21.48(±6.03), and the mean of depression was 53.10(±9.64). The prevalence of depression was mild depression 24.0%, moderate depression 43.5% and severe depression 25.0%. Depression was associated with life satisfaction and depression was higher, life satisfaction was lower. Family function and age were positively associated with life satisfaction, Conclusion: Life satisfaction was affected by depression and family function in hospitalized Vietnam veterans. Therefore, it was thought that psychosocial approaches such as improve depression and family function in care of hospitalized Vietnam veterans is required.