Use of RAPD Analysis to Determine Genetic Diversity and Relationships among Bougainvillea Cultivars at Intra- and Inter-Specific Levels

Jolly Chatterjee,Abul Kalam Azad Mandal,Debasis Chakrabarty,Subodh Kumar Datta 한국원예학회 2007 Horticulture, Environment, and Biotechnology Vol.48 No.1

This study was aimed at determining the genetic relationships among the most important Bougainvillea cultivars grown at the National Botanical Research Institute, Lucknow, India using random amplified polymorphic DNA (RAPD) technology. Fifty random decamer primers were screened and ten were selected for final RAPD analysis. Each primer produced a unique set of amplification products ranging in size from 300 to 2500 bp. The number of bands for each primer varied from 10 in P2 to 18 in P10. These ten primers used in this analysis yielded 167 scorable bands with an average of 11.3 bands per primer. Of the 167 fragments scored from these primers, 26 were monomorphic and 141 were polymorphic (84.4%). The generated similarity matrix showed that the genetic diversity within the tested genotypes was high (average similarity index = 30.1%). Similarity values among the studied genotypes ranged between 6 to 89%. The resulting dendrogram divided the cultivars into two main clusters. The first contained 37 cultivars and was divided into two subclusters at a similarity value of 0.03 with 21 and 16 cultivars in each subcluster, respectively. Subcluster Ⅰ included those cultivars which had affinity with Buttiana group. Subcluster Ⅱ contained cultivars of both Spectabilis and Peruviana groups. The second major cluster contained 55 cultivars, contain species of Glabra group and cultivars whose origin are not well recorded. The similarity data obtained in this study agree to some extent (at least for the Buttiana group) with the previous classification of the tested genotypes. The information obtained from this work may be useful for better management, identification of accessions and also in avoiding duplications or mislabeling of the genotypes studied.

Intravarietal Variation of Miniature Chrysanthemum Cultivars Using Randomly Amplified Polymorphic DNA

Jolly Chatterjee,Abul Kalam Azad Mandal,Sirish Anand Ranade,Subodh Kumar Datta 한국원예학회 2006 Horticulture, Environment, and Biotechnology Vol.47 No.4

Genetic diversity was estimated of 24 miniature chrysanthemum cultivars at intravarietal level first time using randomly amplified polymorphic DNA. A total of 50 random 10-mer primers have been screened of which 13 gave good amplification products. Bands amplified by these 13 selected primers ranged from 5 to 13 in number and 300 bp to 2.239 kb in molecular weight. RAPD marker showed 43-85% similarity among the cultivars. Cluster analysis was done by the Neighbor Joining Tree method which showed two major groups. Cluster 1 consisted of five cultivars, whereas cluster 2 consisted of 19 cultivars. Cluster 2 have two subclusters, one consisted of ten cultivars and the other of nine cultivars. The results provide important information for the identification and estimation of genetic diversity of chrysanthemum cultivars.

Rwanda Tea Production, Consumption, Export and Added Value

Jolly Ninsiima,Ha Sook Chung 한국차학회 2016 한국차학회지 Vol.22 No.4

Tea is an aromatic beverage commonly prepared by pouring hot or boiling water over the cured leaves of Camellia sinensis L. The purpose of this paper is to discuss the consumption, export and added value of tea produced in Rwanda. Although there is little documentation, the trade trends and drinking of Rwandan tea from a Rwandan cultural perspective, this paper used the few publications and websites available on the trends of Rwandan tea industry. In Rwanda, tea is planted on hillsides at high altitude and on well drained marshes. Rwanda is regularly confronted by prolonged droughts (resulting in famine; water sources drying; tendency toward desertification especially in the east and south) and heavy rains, which lead to a rise in the water level, floods, landslides, localized landslides, soil erosion on the basin sides and expansion of the river beds, thus affecting its agriculture production and infrastructure. The teas processed in Rwanda are mainly black tea (CTC), with green tea and orthodox tea being processed in small amounts. As in most places, the economic value of tea products attracts investment. However, there is still a lot to be done, including research and industrial expansion.

의사중심의 의료의 질관리 사업 : 프랑스에서의 경험 a Policy

Giraud, Alexandra,Jolly, Dominique,金昌燁 대한의학협회 1992 대한의학협회지 Vol.35 No.10

A review on numerical models and controllers for biped locomotion over leveled and uneven terrains

Varma, Navaneeth,Jolly, K.G.,Suresh, K.S. Techno-Press 2018 Advances in robotics research Vol.2 No.2

The evolution of bipedal robots was the foundation stone for development of Humanoid robots. The highly complex and non-linear dynamic of human walking made it very difficult for researchers to simulate the gait patterns under different conditions. Simple controllers were developed initially using basic mechanics like Linear Inverted Pendulum (LIP) model and later on advanced into complex control systems with dynamic stability with the help of high accuracy feedback systems and efficient real-time optimization algorithms. This paper illustrates a number of significant mathematical models and controllers developed so far in the field of bipeds and humanoids. The key facts and ideas are extracted and categorized in order to describe it in a comprehensible structure.

A Prospective Study of Anterior Segment Ocular Parameters in Anisometropia

Neha Singh,Jolly Rohatgi,Vinod Kumar 대한안과학회 2017 Korean Journal of Ophthalmology Vol.31 No.2

Purpose: The aim of this study was to investigate the differences in anterior segment ocular parameters inanisometropia >1 D. Methods: This study included 202 eyes of 101 subjects ranging from 10 to 40 years of age with anisometropiaof 1 D or more. The subjects were divided into groups according to anisomyopia, anisoastigmatism, andanisohypermetropia. After providing informed consent, each patient underwent a detailed ophthalmologicalexamination including cycloplegic refraction, best-corrected visual acuity, cover test, axial length (AL) measurementusing A-scan ultrasound biometer, keratometry, anterior chamber depth, and central corneal thicknessmeasurement. For each participant, the eye with greater refractive error was compared to the fellow eyevia paired t-tests. Correlations between parameters were studied using the Pearson correlation coefficient. Results: The average age of subjects was 21.7 ± 9.3 years. Of 101 subjects, 31 had anisomyopia; 42 had anisohypermetropia;and 28 had anisoastigmatism. A predisposition toward greater myopia in right eyes was noted inanisomyopia (24 of 31 subjects, 77%). The inter-ocular acuity difference was significant in all three groups (p < 0.01). As the degree of anisometropia increased, there was significant positive correlation in the difference in AL inmyopes (r = 0.863, p < 0.01) and hypermetropes (r = 0.669, p < 0.01) and the difference in corneal curvaturein anisoastigmatism (r = 0.564, p = 0.002) and hypermetropes (r = 0.376, p = 0.014). A significant positive correlationwas also present between the anterior chamber depth difference and refractive difference in hypermetropes(r = 0.359, p = 0.020). Conclusions: This study showed that anisomyopia is correlated only with anterior chamber differences. Anisohypermetropiais correlated with AL differences as well as corneal curvature difference and anterior chamberdepth difference. The amount of anisoastigmatism correlates only with corneal curvature difference.

Electrospun PVDF/ZnO Based Composite Fibers for Oil Absorption and Photocatalytic Degradation of Organic Dyes from Waste Water

Hemalatha Parangusan,Jolly Bhadra,Zubair Ahmad,Ali S. M A Al-Maadeed,Abdulaziz M. A A Al-Mohannadi,Noora Al-Thani 한국섬유공학회 2022 Fibers and polymers Vol.23 No.5

Clean drinking water has been a vital topic of research in modern world, this makes efficient water purification asthe latest demand. The research and development in the field of filtration technology has revitalize considerable awareness indifferent engineered methods and nanomaterials. In this work, a hydrophobic composite fibers was successfully prepared byincorporating ZnO nanofiller into polyvinylidene fluoride (PVDF) using an electrospinning method for organic dyedegradation and oil absorption applications. The hydrothermal method was used to prepare pure ZnO. The structuralproperties of PVDF/ZnO composite fibers and pure ZnO were studied using the X-ray diffraction technique (XRD). TheSEM image of pure ZnO shows a flower-like structure and the composites exhibited the structure of the fibers. The averagefiber diameter of the pure PVDF fiber was around 625 nm and the PVDF/ZnO composite fiber was around 485 nm,respectively. Finally, the prepared fibers were tested for oil absorption and the photocatalytic degradation of organic dyes. The photocatalytic activity of PVDF/ZnO was evaluated by the degradation of Azocarmine G (AZG) and Malachite green(MG) dye under sunlight irradiation. The results showed that 85 % of AZG and 90 % of MG dye could be degraded within120 min and 240 min. It was found that the PVDF/ZnO composite fiber was hydrophobic (135 °) under water. Good oilabsorption efficiency (115 %) was achieved using PVDF/ZnO composite fibers. The results shows that the preparedcomposite fibers can be used to absorb oil and degrade organic contaminants. This cost-effective, easy operation, reusability,and efficiency of the PVDF/ZnO fiber mats could be potentially useful for water treatment and oil recovery.

Seismo-acoustic characterization of the 2018 Ambae eruption, Vanuatu

Iseul Park,Arthur Jolly,Robin Matoza,Ben Kennedy,Geoff Kilgour,Richard Johnson,Esline Garaebiti,Sandrine Cevuard 대한지질학회 2021 대한지질학회 학술대회 Vol.2021 No.10

Ambae (also called Manaro Voui or Aoba) is a basaltic shield volcano in the Vanuatu archipelago. Recently, this volcano resumed a new eruptive episode from 2017 and released various seismic and acoustic activity associated with phreatic/phreatomagmatic/magmatic eruptions. To observe the activity, we installed a multi-station network comprising seven broadband seismic stations and four 3-element acoustic arrays during the 2018 eruptive phase. The observed seismic signals mostly consist of volcanic tremor and explosion quakes, which are related to volcanic fluid movement. The corresponding acoustic (infrasound) signals have relatively low frequency (< 1 Hz) and affect the seismic data in a very-long-period band as ground-coupled airwaves. The infrasound waves during volcanic explosions show strong coherence over all the arrays, and these coherent signals are used to detect previously unreported volcanic explosions based on a reverse-time-migration method. During the detected explosions, seismic source locations ascend and then descend, which might be interpreted as the propagation of a pressure front, bubble activity, and two superimposed source locations. No preceding study for Ambae’s eruption has been completed, hence this study using novel seismo-acosutic dataset may be useful to improve the local monitoring system and future work.

The learning formats of coach education materials

John Lyle,Sue Jolly,Julian North 한국코칭능력개발원 2010 International Journal of Coaching Science Vol.4 No.1

This paper reports on an investigation into the design of coach education materials. At a time of significant change in coach education in the UK, it was appropriate to examine the extent to which coach education materials conform to good practice in terms of adult learning design principles and the formatting of the content. Coach education materials from Levels 1-3 of coaching awards in 5 sports were content analysed. These data were accompanied by opinions from 60 coaches and tutors, a focus group, and number of interviews. In the absence of a substantial literature on this aspect of coach education, a framework of analysis criteria was derived from the adult learning literature and prescriptions of good practice from those who had substantial experience in materials design. The framework elements (each with sub-groups of 5 to 18 items) were progression, feedback, interactivity, reflection, operation, contextualisation, summarising, attractiveness, layout & structure, and accessibility. The coach education materials were mixed in quality, and the analyses were usefully focused on two issues - presentational formatting and learning principles design (structural formatting). Overall, interactivity, reflection and feedback in the learning materials were less positive than might have been expected. The project was able to provide a valuable set of operational criteria for guiding the future production of learning materials in coach education.

Formulation consideration and skin retention study of microemulsion containing tazarotene for targeted therapy of acne

Mrunali R. Patel,Rashmin B. Patel,Jolly R. Parikh,Bharat G. Patel 한국약제학회 2016 Journal of Pharmaceutical Investigation Vol.46 No.1

The objective of this study was to develop and evaluate a novel microemulsion (ME) formulation containing tazarotene for targeted topical therapy of acne. Microemulsions (MEs) were formulated by spontaneous microemulsification method using 12 % Isopropyl myristate, mixed emulsifiers 15 % Labrasol-Cremophor-RH 40 (1:1), 15 % Capmul MCM and 58 % water (w/w) as an external phase. All plain and tazarotene loaded MEs were clear and showed physicochemical parameters for desired topical delivery and stability. The permeation profile of tazarotene through rat skin from selected ME formulation exhibited highest skin uptake. The microscopic observations indicated that the optimized ME had no significant effect on the microscopic structure of the skin and epithelial cells appeared mostly unchanged. The surface epithelium lining and the granular cellular structure of the skin were totally intact. The developed ME may be a potential drug delivery vehicle for targeted topical delivery of tazarotene in the treatment of acne.