Anti-intestinal inflammatory activity of fucoidan extracted from Hizikia fusiformis depending on extraction process

Sung Bum Son,Seung Joo Kim,Jiyoon Kim,Jun Seong Kwon,Sung-Hwan Eom,Prakash Chaudhary,Jung-Ae Kim,Kyung Tae Kim 한국수산과학회 양식분과 2023 한국수산과학회 양식분과 학술대회 Vol.2023 No.6

Bardoxolone Methyl Suppresses Hepatitis B Virus Large Surface Protein Variant W4P-Related Carcinogenesis and Hepatocellular Carcinoma Cell Proliferation Via the Inhibition of Signal Transducer and Activator of Transcription 3 Signaling

Gee, Min Sung,Kang, Sung-Bae,Kim, Namkwon,Choi, Jiyoon,Kim, Nam-Jung,Kim, Bum-Joon,Inn, Kyung-Soo,Lee, Jong Kil S. Karger 2018 Pharmacology Vol. No.

<P>Bardoxolone methyl (CDDO-me) is a synthetic triterpenoid that has been shown to suppress various cancers and inflammation. It has been implicated for the suppression of signal transducer and activator of transcription 3 (STAT3)-mediated signaling, which plays crucial roles in the development and progression of hepatocellular carcinoma (HCC). Previously, we showed that hepatitis B virus (HBV) large surface protein (LHB) variant W4P promotes carcinogenesis and tumor progression through STAT3 activation. Thus, we examined the anti-cancer activity of CDDO-me against HCC using W4P-LHB-expressing NIH3T3 cells and HepG2 and Huh7 HCC cell lines. CDDO-me exerted cytotoxic activity against W4P-LHB-expressing NIH3T3 cells, HepG2 cells, and Huh7 cells, and induced apoptotic cell death in a dose-dependent manner, demonstrating its anti-cancer activity against HCC. Sublethal concentrations of CDDO-me suppressed STAT3 activation by W4P-LHB ectopic expression and interleukin-6 treatment in W4P-LHB-NIH3T3 and Huh7 cells respectively. The suppression of STAT3 activation by CDDO-me in W4P-LHB-NIH3T3 cells was further confirmed by decreased cyclin D1 protein levels and increased p21 and p53 mRNA synthesis. In addition, CDDO-me treatment resulted in decreased cell migration and colony formation in in vitro assays using W4P-LHB-NIH3T3, HepG2, or Huh7 cell lines, supporting its anti-cancer activity through STAT3 inhibition. Furthermore, ­CDDO-me administration significantly suppressed tumor growth induced by W4P-LHB-expressing NIH3T3 cells in nude mice, confirming its anti-cancer activity. Collectively, our findings demonstrated that CDDO-me is capable of suppressing STAT3 activation in HCC cells and cells transformed by the natural variant of HBV protein. The results suggest that CDDO-me can be a potential therapeutic agent against HCC, especially tumors related to HBV mutations.</P>

Anti-Inflammatory Effect of Essential Oils Extracted from Wood of Four Coniferous Tree Species (침엽수 4종 목부 정유의 항염증 효과 평가)

( Jiyoon YANG ),( Won-Sil CHOI ),( Jae-Woo KIM ),( Sung-Suk LEE ),( Mi-Jin PARK ) 한국목재공학회 2019 목재공학 Vol.47 No.6

본 연구는 국내산 편백, 소나무, 잣나무, 일본잎갈나무 목부 정유의 항염증 효과를 평가 하였다. 실험에 사용된 정유는 각 수종의 목부에서 증류법(hydrodistillation)으로 추출되었다. 추출한 정유들을 GC-MS를 통해 성분 분석한 결과, 편백의 경우 α-cadinol (19.25%), τ-muurolol (14.20%) 및 α-pinene (13.74%)이, 소나무는 α-pinene (47.16%), longifolene (14.31%), β-phellandrene (11.78%) 및 β-pinene (11.02%)이 주요성분이었으며, 잣나무의 경우 α-pinene (13.49%)와 longifolene (10.79%), 낙엽송의 경우 geranyl linalool (23.58%) 및 α-pinene (18.57%)이 주요성분으로 확인되었다. 침엽수 목부 정유들의 항염증 효과는 LPS(Lipopolysaccharide)로 염증반응이 유도된 RBL-2H3 비만세포주에 정유를 처리한 후 IL-4(interleukin-4), IL-13(interleukin-13), β-hexosaminidase의 발현 변화를 조사함으로써 평가하였다. MTT assay를 통한 세포 독성 평가 결과, 침엽수 4종 정유 모두 10-7~10-5% 농도에서 세포 독성을 보이지 않았다. 염증반응이 유도된 RBL-2H3 세포에 침엽수 4종의 목부 정유를 10-7% 농도로 처리하였을 때, LPS 처리군 대비 침엽수 4종의 목부 처리군에서 IL-4와 IL-13의 상대적 발현량이 큰 폭으로 감소하였으며, β-hexosaminidase 분비가 유의하게 억제되었다. 침엽수 정유 4종의 IL-4 상대적 발현량은 LPS 처리군 대비 소나무 69.6%, 잣나무 63.2%, 편백 55.1% 그리고 낙엽송 45.8% 감소하였다. IL-13의 상대적 발현량은 소나무 57.8%, 잣나무 57.1%, 편백 51.1% 그리고 낙엽송 34.5% 감소하였다. β-Hexosaminidase 분비의 경우 LPS 처리군 대비 편백 38.1%, 소나무 33.0%, 잣나무 27.4% 그리고 낙엽송 9.1% 억제하였다. Il-4, IL-13 및 β-hexosaminidase 분비를 비교한 결과, 침엽수 목부 정유 4종 중 소나무 정유의 활성이 가장 우수하였다. 이러한 결과는 국내 침엽수 4종의 목부 정유는 염증반응을 유발하는 싸이토카인의 발현 억제를 통하여 항염증 효과를 나타냄을 확인할 수 있었다. The aim of this study was to evaluate the anti-inflammatory effects of essential oils extracted from the wood of Chamaecyparis obtusa, Pinus densiflora, Pinus koraiensis, and Larix kaempferi. Essential oils were extracted by hydrodistillation, and their chemical components were determined by GC/MS. Major chemical components of these essential oils were α-cadinol (19.25%), τ-muurolol (14.20%), and α-pinene (13.74%) in C. obtusa; α-pinene (47.16%), longifolene (14.31%), β-phellandrene (11.78%), and β-pinene (11.02%) in P. densiflora; α-pinene (13.49%) and longifolene (10.79%) in P. koraiensis, and geranyl linalool (23.58%) and α-pinene (18.57%) in L. kaempferi. To evaluate the anti-inflammatory effects of essential oils, lipopolysaccharide (LPS)-induced RBL-2H3 mast cells were treated with these essential oils; then, the changes in the mRNA expression level of the cytokines IL-4 and IL-13 were examined. Further, degranulation was evaluated by measuring β-hexosaminidase release. After LPS-induced RBL-2H3 mast cells were exposed to 10-7% of all types of essential oils, the gene expression levels of IL-4 and IL-13 within the cells remarkably decreased. The relative mRNA expression level of IL-4 was 69.6% in P. densiflora, 63.2% in P. koraiensis, 55.1% in C. obtusa, and 45.8% in L. kaempferi compared with that in the group treated with LPS. The mRNA expression level of L-13 should a similar trend. The inhibitory rate of IL-13 mRNA expression of P. densiflora, P. koraiensis, C. obtusa, and L. kaempferi was 57.8%, 57.1%, 51.1%, and 34.5%, respectively. β-Hexosaminidase release significantly decreased following the treatment with the four types of essential oils. The rate of β-hexosaminidase release were 38.1% C. obtusa; 33.0% P. densiflora; 27.4% P. koraiensis; and 9.1% L. kaempferi. Among all types of essential oils, that extracted from P. densiflora wood showed the highest anti-inflammatory activity. These results show that the tested essential oils exert an anti-inflammatory effect through the inhibition of degranulation and expression of cytokines.

응급실 손상환자 심층조사 자료를 이용한 2017-2018년 중독 환자의 분석

고지윤 ( Jiyoon Koh ),전우찬 ( Woochan Jeon ),강형구 ( Hyunggoo Kang ),김양원 ( Yang Weon Kim ),김현 ( Hyun Kim ),오범진 ( Bum Jin Oh ),이미진 ( Mi Jin Lee ),전병조 ( Byeong Jo Chun ),정성필 ( Sung Phil Chung ),김경환 ( Kyung Hwa 대한임상독성학회 2020 대한임상독성학회지 Vol.18 No.2

Purpose: The annual statistics for poisoning are reported based on the data from poison control centers in many advanced countries. In 2016 a study was conducted to analyze the 2016 Korea Poisoning status. This study was conducted to make a better annual report for poisoning statistics in Korea from a 2017-2018 national representative database. Methods: This study was a retrospective analysis of poisoning patients based on the data from an emergency department (ED) based injury in-depth surveillance project by the Korea Centers for Disease Control and Prevention in 2017-2018. Bite or sting injuries were not included. Results: A total of 17714 patients presented to 23 EDs because of poisoning. Adults above 20 years old age accounted for 84.6% of the population, while the proportion of intentional poisoning was 60.8%. The poisoning substance presented in the ED were therapeutic drugs (51.2%), gas (20.3%), pesticides (16.4%), and artificial substances (11.4%). Overall, 35% of patients were admitted for further treatment. The mortality was 2.4% (422 cases), and the most common fatal substances in order were carbon monoxide, other herbicides, and paraquat. Conclusion: This study showed the 2017-2018 status of poisoning in Korea. The prognosis is different from the cause of poisoning and the initial mental state of the patient. Therefore, appropriate methods for preventing poisoning and therapeutic plans in specific situations are needed.

미이용 산림바이오매스 공급에 있어 수확벌채의 원목 혼입량 추정

양지윤(Jiyoon Yang),이재정(Jaejung Lee),정한섭(Hanseob Jeong),한상훈(Sang Hun Han),이수민(Soo Min Lee) 한국신재생에너지학회 2022 신재생에너지 Vol.18 No.4

To respond to global warming, there is an increasing interest in eco-friendly alternative energy sources. Therefore, unused forest biomass that has been neglected due to a lack of marketability is attracting attention. With the introduction of the “unused forest biomass certification system” in 2019, ways of determining quantity of unused forest biomass have steadily increased. However, there have been reported cases whereby unused forest biomass weighed more than the amount of harvested trees. It was found that it was possible that forest resources that can be used as round wood were mixed with unused forest biomass. In this context, this study aimed to estimate the amount of mixed round wood in the unused forest biomass supply. The relative expression of growing stock/ha versus the amount of final clearing/ha collected was modeled (y=1.490x-94.341, R²=0.861). As a result, it was found that round wood was mixed into the unused forest biomass, contributing to the disparity observed between the weighted forest biomass and the amount of trees harvested. In conclusion, proper declaration and certification procedures should be carried out for the use of forest resources and promoting unused forest biomass usage.

기상 단기 예보에 대한 수반 모델 기반의 관측 영향

김성민(Sung-Min, Kim),방철한(Cheolhan, Bang),황진주(JinJoo, Hwang),신명진(MyeongJin, Shin),오예슬(Ye Seul, Oh),신지윤(JiYoon Shin),이강민(KangMin, Lee),이현정(HyunJung, Lee) 대한환경공학회 2020 대한환경공학회 학술발표논문집 Vol.2020 No.11

< 전시-P-78 > 국내산 침엽수 4종 정유의 항천식 효과 평가

양지윤 ( Jiyoon Yang ),박미진 ( Mi-jin Park ),최원실 ( Won-sil Choi ),김재우 ( Jae-woo Kim ),이성숙 ( Sung-suk Lee ) 한국목재공학회 2019 한국목재공학회 학술발표논문집 Vol.2019 No.1

본 연구는 국내산 침엽수인 편백(Chamaecyparis obtusa), 소나무(Pinus densiflora), 잣나무(Pinus koraiensis), 일본잎갈나무(Larix kaempferi) 목부 정유의 항천식 효과를 평가하였다. 실험에 사용된 정유는 침엽수 4종의 목부를 분쇄하여 증류법(hydrodistillation)으로 추출하였다. 추출한 침엽수 4종의 목부 정유는 GC-MS(Trace 1310/ISQ-LT, ThermoScientific, USA)를 이용하여 분석하였다. 정유의 성분분석 결과, 주요성분으로 편백 15종, 소나무 19종, 잣나무 17종, 일본잎갈나무 18종이 검출되었으며, α-pinene, limonene, α-terpineol과 bornyl acetate 성분은 4수종 모두 공통적으로 검출되었다. 편백 정유의 경우 α-cadinol(15.90%), τ-muurolol(10.18%), δ-cadinene(8.18%)이, 소나무 정유의 경우 α-pinene(25.06%), β-phellandrene(11.16%), longifolene(10.41%)이, 잣나무 정유의 경우 3-carene(22.03%), α-pinene(15.70%)이, 일본잎갈나무 정유의 경우 α-pinene(27.06%), geranyl linalool(8.78%), β-pinene(7.84%)이 높은 비율을 차지하였다. 침엽수 4종 목부 정유들의 항천식 효과는 ovalbumin으로 염증반응이 유도된 NCI-H292 기관지 상피세포에 정유를 처리한 후 IL-8, IL-1β의 발현 변화를 조사함으로써 평가하였다. CCK assay를 통해 세포독성을 평가한 후 목부 정유의 처리 농도를 10^-6%로 결정하였다. 염증반응이 유도된 NCI-H292 세포에 침엽수 4종의 목부 정유를 10^-6% 농도로 처리하자 ovalbumin 처리군 대비 침엽수 4종의 목부 정유 처리군에서 IL-8과 IL-1β의 상대적 발현량이 큰 폭으로 감소하였다. 침엽수 4종의 IL-8 상대적 발현량은 ovalbumin 처리군 대비 편백 33.2%, 소나무 32.8%, 일본잎갈나무 19.7%, 잣나무 14.9% 감소하였다. IL-1β의 상대적 발현량은 편백 64.2%, 소나무 60.7%, 일본잎갈나무 56.2%, 잣나무 52.8% 감소하였다. IL-8과 IL-1β의 발현억제를 비교한 결과, 침엽수 4종 중 편백 목부 정유의 활성이 가장 우수하였다. 또한 목부 정유는 IL-8보다는 염증반응을 증폭시키는데 관여하는 IL-1β의 발현을 보다 더 효과적으로 억제하는 것으로 조사되었다. 따라서, 목부 정유 성분들은 염증반응의 증폭을 억제하는데 더 영향을 줌으로써 항천식 효과를 보이는 것으로 사료된다.

Targeting Risk Factors for the Control of Central Line-Associated Bloodstream Infection in the Neonatal Intensive Care Unit: A Single Tertiary Center Experience

Jeong, Jiyoon,Kwun, Yoojin,Kim, Min-ju,Choi, Sang-Ho,Jung, Euiseok,Lee, Byong Sop,Kim, Ki-Soo,Kim, Ellen Ai-Rhan The Korean Society of Neonatology 2021 Neonatal medicine Vol.28 No.3

Purpose: The aim of this study was to estimate the effect of targeting risk factors for the control of central line-associated bloodstream infection (CLABSI) among high-risk infants in a tertiary neonatal intensive care unit (NICU). Methods: Infants admitted to the NICU and diagnosed with CLABSI from January to December 2013 were eligible for inclusion to the study. The CLABSI group (n=47) was matched in a 1:2 ratio to the control group (n=94) based on gestational age, birth weight, and Score for Neonatal Acute Physiology-II. Risk factors for CLABSI were identified using the Cox proportional hazard model, and analysis of the effect of these risk factors targeting infection control was performed. Results: The risk factors associated with CLABSI were prolonged central line dwell days (adjusted hazard ratio [HR], 1.028; 95% confidence interval [CI], 1.011 to 1.045; P=0.001), use of a silicone catheter (adjusted HR, 5.895; 95% CI, 1.893 to 18.355; P=0.002), surgical treatment (adjusted HR, 3.793; 95% CI, 1.467 to 9.805; P=0.006), and less probiotic supplementation (adjusted HR, 0.254; 95% CI, 0.068 to 0.949; P=0.042). By targeting these risk factors with a quality improvement initiative, the mean CLABSI incidence rate per 1,000 catheter-days decreased from 6.6 to 3.1 (P=0.004). Conclusion: Targeting risk factors for infection control significantly reduced the rate of CLABSI among high-risk infants in the NICU.

A Stator Winding Insulation Condition Monitoring Technique for Inverter-Fed Machines

Jinkyu Yang,Sang Bin Lee,Jiyoon Yoo,Sanghoon Lee,Yongmin Oh,Changho Choi IEEE 2007 IEEE transactions on power electronics Vol.22 No.5

<P>Stator insulation quality assessment is an important issue for pulsewidth modulation inverter-fed machines, since the stator insulation is exposed to increased thermal/electrical stresses. In this paper, a new approach for monitoring the condition of the stator insulation for inverter-fed machines is proposed. The main concept of the proposed approach is to apply various types of test voltages to the motor stator insulation using the inverter, to perform standard offline insulation tests whenever the motor is not operating. Two methods for testing the insulation with modifications to the inverter circuit are proposed and verified experimentally. The results show that the proposed technique provides a simple low cost solution for detecting stator insulation degradation at an early stage for reliable operation of inverter-fed machine systems, since the insulation condition can be monitored frequently. This not only helps prevent forced outages and safety risks due to insulation breakdown, but also helps perform maintenance in an efficient manner since individual motor maintenance can be prioritized and scheduled based on its present condition.</P>

Protocol for the Observation of the Primo Vascular System in the Lymph Vessels of Rabbits

JUNG SHARON JIYOON,Kyoung-Hee Bae,Sang Yeon Cho,Sun Hee Hwang,이병천,김성철,권병세,Hee Min Kwon,송윤규,소광섭 사단법인약침학회 2012 Journal of Acupuncture & Meridian Studies Vol.5 No.5

Molecular-level understanding of the structure and the functions of the lymphatic system has greatly enhanced the importance of this second circulation system, especially in connection with cancer metastasis and inflammation. Recently, a third circulatory system, the primo vascular system (PVS) was found in various parts of an animal’s body, especially as threadlike structures floating in the lymphatic flow in lymph vessels. Although the medical significance of this emerging system will require much work in the future, at present, several important suggestions in connection with immune cells, stem cells, and cancer metastasis have already appeared. Experiments to observe the PVS in the lymph vessels near the caudal vena cava of rabbits and rats have been performed by several independent teams, but reproduction requires considerable skill and technical know-how. In this article, we provide a detailed protocol to detect the PVS inside the lymph vessels of a rabbit. Detection and isolation are the first steps in unraveling the physiological functions of the PVS, which awaits intensive research.