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Effect of Conversion to CTLA4Ig on Tacrolimus-Induced Diabetic Rats
Jin, Long,Lim, Sun Woo,Jin, Jian,Luo, Kang,Ko, Eun Jeong,Chung, Byung Ha,Lin, Hong Li,Yang, Chul Woo Wolters Kluwer Health, Inc. All rights reserved. 2018 Transplantation Vol.102 No.4
<P>Conclusions CTLA4Ig was not diabetogenic, and conversion to CTLA4Ig reduced TAC-induced pancreatic islet injury.</P>
<i>Caulobacter daechungensis</i> sp. nov., a stalked bacterium isolated from a eutrophic reservoir
Jin, Long,Lee, Hyung-Gwan,Kim, Hee-Sik,Ahn, Chi-Yong,Oh, Hee-Mock International Union of Microbiological Societies 2013 International journal of systematic and evolutiona Vol.63 No.7
<P>A Gram-stain-negative, aerobic, non-spore-forming, curved, rod-shaped bacterium, H-E3-2<SUP>T</SUP>, was isolated from a water sample taken from Daechung Reservoir, Republic of Korea, during the late-blooming period of cyanobacteria. Strain H-E3-2<SUP>T</SUP> was motile with a single polar flagellum or non-motile (stalked cell). Comparative 16S rRNA gene sequence studies showed the isolate had a clear affiliation with the class <I>Alphaproteobacteria</I> and was most closely related to <I>Caulobacter fusiformis</I> ATCC 15257<SUP>T</SUP> and <I>Caulobacter mirabilis</I> LMG 24261<SUP>T</SUP>, showing 97.6 and 97.3 % 16S rRNA gene sequence similarity, respectively, and 95.3–96.3 % similarity to all other species of the genus <I>Caulobacter</I>. The predominant ubiquinone was Q-10. The major fatty acids were summed feature 8 (C<SUB>18 : 1</SUB>ω6<I>c</I> and/or C<SUB>18 : 1</SUB>ω7<I>c</I>) and C<SUB>16 : 0</SUB>. The G+C content of the genomic DNA of strain H-E3-2<SUP>T</SUP> was 64.7 mol%. DNA–DNA hybridization values of strain H-E3-2<SUP>T</SUP> with <I>C. fusiformis</I> ATCC 15257<SUP>T</SUP> and <I>C. mirabilis</I> LMG 24261<SUP>T</SUP> were 21.2 and 19.7 %, respectively. Thus, based on the results of polyphasic analysis, it is proposed that strain H-E3-2<SUP>T</SUP> represents a novel species of the genus <I>Caulobacter</I>, for which the name <I>Caulobacter daechungensis</I> sp. nov. is proposed. The type strain is H-E3-2<SUP>T</SUP> ( = KCTC 32211<SUP>T</SUP> = JCM 18689<SUP>T</SUP>).</P>
Jin, Long,Huy, Hangsak,Kim, Kwang Kyu,Lee, Hyung-Gwan,Kim, Hee-Sik,Ahn, Chi-Yong,Oh, Hee-Mock International Union of Microbiological Societies 2013 International journal of systematic and evolutiona Vol.63 No.8
<P>A novel Gram-reaction-positive bacterium, strain CH22-21<SUP>T</SUP>, was isolated from a water sample taken from Daechung Reservoir, Republic of Korea, during the late-blooming period of cyanobacteria. Cells of strain CH22-21<SUP>T</SUP> were non-motile, ciliated short rods that formed creamy-white colonies on half-strength modified R2A agar. Chemotaxonomic results showed menaquinone MK-9(H<SUB>6</SUB>) as the predominant respiratory menaquinone, diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositolmannoside as major polar lipids, 16 : 1ω5<I>c</I>, 16 : 0, 17 : 1ω8<I>c</I> and 18 : 1ω9<I>c</I> as major fatty acids, and a DNA G+C content of 71.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the strain formed a separate lineage within the order <I>Acidimicrobiales</I>, showing similarity values of <92.3 % with its closest phylogenetic neighbours. The combined genotypic and phenotypic data showed that strain CH22-21<SUP>T</SUP> could be distinguished from all genera within the order <I>Acidimicrobiales</I> and represented a novel species of a new genus in the family <I>Iamiaceae</I>, for which the name <I>Aquihabitans dachungensis</I> gen. nov., sp. nov. is proposed. The type strain of <I>Aquihabitans dachungensis</I> is CH22-21<SUP>T</SUP> ( = KCTC 19849<SUP>T</SUP> = JCM 17787<SUP>T</SUP>).</P>
Study of Application of PTFE Fiber in Self-cleaning Filter Media
Jin Long,Min Tang,Yun Liang,Jian Hu 한국섬유공학회 2018 Fibers and polymers Vol.19 No.12
To provide protection against the adverse effects of high concentration dust, self-cleaning filter was applied in the air inlet system of engine and gas turbine. PTFE fiber could have a great application in self-cleaning filter media due to its low surface free energy. In this work, new PTFE/cellulose fiber composite media for self-cleaning filter were prepared by wet-laid method. The effect of PTFE fiber on physical properties and filtration performance were studied. It was found that PTFE fiber was well dispersed in water without bubble formation after proper surfactant was applied. The initial figure of merit increased significantly when percentage of PTFE fiber was higher. For self-cleaning performance, particles were easier to be detached from composite media with higher PTFE fiber percentage, which resulted in lower average pressure drop. It was estimated that in the loading process, energy consumption of media containing 30 % and 60 % PTFE fiber was 32 % and 47 % less than that of pure cellulose media, respectively. PTFE fiber significantly improved the self-cleaning performance due to lower surface free energy of composite media and higher efficiency per unit thickness.
<i>Flaviflexus salsibiostraticola</i> sp. nov., an actinobacterium isolated from a biofilm reactor
Jin, Long,Ko, So-Ra,Lee, Hyung-Gwan,Kim, Byung-Hyuk,Kim, Hee-Sik,Ahn, Chi-Yong,Oh, Hee-Mock International Union of Microbiological Societies 2014 International journal of systematic and evolutiona Vol.64 No.9
<P>A Gram-stain-positive, aerobic, non-motile, non-spore-forming, cocci-shaped actinobacterium, designated strain EBR4-1-2<SUP>T</SUP>, was isolated from a biofilm reactor in Korea. Comparative 16S rRNA gene sequence studies showed the isolate was clearly affiliated with the class <I>Actinobacteria</I>, and was related most closely to <I>Flaviflexus huanghaiensis</I> H5<SUP>T</SUP>, showing 98.9 % similarity. Cells of strain EBR4-1-2<SUP>T</SUP> formed yellow colonies on R2A agar, contained MK-9(H<SUB>4</SUB>) as the predominant menaquinone, and included C<SUB>18 : 1</SUB>ω9<I>c</I>, C<SUB>16 : 0</SUB>, C<SUB>16 : 1</SUB>ω9<I>c</I> and C<SUB>14 : 0</SUB> as the major fatty acids. The cell-wall peptidoglycan type was A5α (<SMALL>l</SMALL>-Lys–<SMALL>l</SMALL>-Ala–<SMALL>l</SMALL>-Lys–<SMALL>d</SMALL>-Glu). The G+C content of the genomic DNA of strain EBR4-1-2<SUP>T</SUP> was 65.6 mol%. Thus, the combined genotypic and phenotypic data supported the conclusion that strain EBR4-1-2<SUP>T</SUP> represents a novel species of the genus <I>Flaviflexus</I>, for which the name <I>Flaviflexus</I> <I>salsibiostraticola</I> sp. nov. is proposed. The type strain is EBR4-1-2<SUP>T</SUP> ( = KCTC 33148<SUP>T</SUP> = JCM 19016<SUP>T</SUP>).</P>
<i>Caulobacter</i> <i>profunda</i> sp. nov., isolated from deep freshwater sediment
Jin, Long,La, Hyun-Joon,Lee, Hyung-Gwan,Lee, Jay Jung,Lee, Sanghyup,Ahn, Chi-Yong,Oh, Hee-Mock International Union of Microbiological Societies 2014 International journal of systematic and evolutiona Vol.64 No.3
<P>The Gram-stain-negative, aerobic, non-spore-forming, motile, with a single polar flagellum, or non-motile (stalked) and rod-shaped bacteria, DS48-5-2<SUP>T</SUP> and DS48-6-3, were isolated from a sediment sample collected from a depth of 48 m taken from Daechung Reservoir, Republic of Korea. Comparative 16S rRNA gene sequence studies showed that the two isolates had clear affiliation with <I>Alphaproteobacteria</I> and the closest relatedness to <I>Caulobacter mirabilis</I> FWC 38<SUP>T</SUP>, <I>Caulobacter fusiformis</I> ATCC 15257<SUP>T</SUP> and <I>Caulobacter daechungensis</I> H-E3-2<SUP>T</SUP> showing 98.5 %, 97.3 % and 97.3 % 16S rRNA gene sequence similarity, respectively, and 96.1–96.7 % similarity to all other species of the genus <I>Caulobacter</I>. The two isolates shared 100 % 16S rRNA gene sequence similarity. The predominant ubiquinone was Q-10. The major fatty acids were summed feature 8 (C<SUB>18 : 1</SUB>ω6<I>c</I> and/or C<SUB>18 : 1</SUB>ω7<I>c</I>), C<SUB>16 : 0</SUB>, C<SUB>18 : 0</SUB>ω7<I>c</I> 11-methyl and summed feature 3 (C<SUB>16 : 1</SUB>ω6<I>c</I> and/or C<SUB>16 : 1</SUB>ω7<I>c</I>). The G+C contents of the genomic DNA of strains DS48-5-2<SUP>T</SUP> and DS48-6-3 were 66.7 mol% and 66.2 mol%, respectively. DNA–DNA hybridization values of strains DS48-5-2<SUP>T</SUP> and DS48-6-3 with <I>C. mirabilis</I> FWC 38<SUP>T</SUP>, <I>C. fusiformis</I> ATCC 15257<SUP>T</SUP> and <I>C. daechungensis</I> H-E3-2<SUP>T</SUP> were 19.3 %–24.4 %. Thus, based on the evidence from polyphasic studies, it is proposed that strains DS48-5-2<SUP>T</SUP> and DS48-6-3 are representatives of a novel species in the genus <I>Caulobacter</I>, for which the name <I>Caulobacter</I> <I>profunda</I> sp. nov. is proposed. The type strain is DS48-5-2<SUP>T</SUP> ( = KCTC 32480<SUP>T</SUP> = JCM 19440<SUP>T</SUP>).</P>
Jin, Long,Kim, Kwang Kyu,Baek, Sang-Hoon,Lee, Sung-Taik Society for General Microbiology 2011 International journal of systematic and evolutiona Vol.61 No.11
<P>Two strains, designated B1-1(T) and B6-8(T), were isolated from the Geumho River and the Dalseo Stream in Korea. Comparative 16S rRNA gene sequence analysis showed a clear affiliation of these two bacteria with the class Alphaproteobacteria, their closest relatives being Kaistia adipata KCTC 12095(T), Kaistia granuli KCTC 12575(T), Kaistia soli KACC 12605(T) and Kaistia terrae KACC 12910(T) with 16S rRNA gene sequence similarities of 95.3 -97.7 % to the two novel strains. Strains B1-1(T) and B6-8(T) shared a 16S rRNA gene sequence similarity value of 96.1 %. Cells of the two strains were Gram-reaction-negative, aerobic, non-motile, short rods or cocci. The predominant ubiquinone was Q-10. The major fatty acids were C(16 : 0,) C(18 : 1)ω7c, C(18 : 0) and C(19 : 0)ω8c cyclo for strain B1-1(T) and C(16 : 0,) C(18 : 1)ω7c, C(18 : 0), C(18 : 1) 2-OH, and C(19 : 0)ω8c cyclo for strain B6-8(T). The G+C contents of the genomic DNA of the strains B1-1(T) and B6-8(T) were 61.6 and 66.5 mol%, respectively. Based on the results of this polyphasic study, strains B1-1(T) ( = KCTC 12849(T) = DSM 18799(T)) and B6-8(T) ( = KCTC 12850(T) = DSM 18800(T)) represent two novel species of the genus Kaistia, for which the names Kaistia geumhonensis sp. nov. and Kaistia dalseonensis sp. nov. are proposed, respectively.</P>