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Jinjia He,Yi Chen,Luyao Dai,Jiachao Yao,Yu Mei,Dzmitry Hrynsphan,Savitskaya Tatsiana,Jun Chen 한국생물공학회 2020 Biotechnology and Bioprocess Engineering Vol.25 No.4
Acrylic acid is used in various industrial applications but inflicts harm to human health and causes environmental pollution. A new bacterium, identified as Rhodococcus ruber JJ-3, was isolated, which can degrade high concentrations of acrylic acid rapidly and completely. Experimental results showed that the strain can achieve complete degradation of 1000 mg·L−1 acrylic acid in 11 h under the following conditions: pH 7, temperature 35°C, and inoculation quantity 15%. A high concentration of acrylic acid (2000 mg·L−1) can be completely removed in 28 h. According to the Monod model, the maximum specific degradation rate (vmax) and half saturation rate constant (KS) of the strain were 0.85 h−1 and 101.83 mg·L−1, respectively. The results of carbon balance revealed that 54.6% carbon was assimilated by R. ruber JJ-3 as biomass, and 43.0% carbon was mineralized into CO2. Furthermore, glycerol and lactic acid were measured as intermediates, and the possible degradation pathway was proposed during the biodegradation of acrylic acid. These results suggested that R. ruber JJ-3 completely degrades acrylic acid and might have a potential environmental implication in the purification of acrylic acid-contaminated environments.
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Lei Shi,Xiaolong Wang,Jinjia Wang,Ping Zhang,Fei Qi,Menghao Cai,Yuanxing Zhang,Xiangshan Zhou 한국유전학회 2018 Genes & Genomics Vol.40 No.4
Two catabolite repressor genes (MIG1 and MIG2) were previously identified in Pichia pastoris, and the derepression of alcohol oxidase (AOX) expression was realized in Δmig1 or Δmig1Δmig2 mutants grown in glycerol, but not in glucose. In this study, genome-wide RNA-seq analysis of Δmig1Δmig2 and the wild-type strain grown in glycerol revealed that the expression of numerous genes was greatly altered. Nearly 7% (357 genes) of approximately 5276 genes annotated in P. pastoris were significantly upregulated, with at least a two-fold differential expression in Δmig1Δmig2; the genes were mainly related to cell metabolism. Approximately 23% (1197 genes) were significantly downregulated; these were mainly correlated with the physiological characteristics of the cell. The methanol catabolism and peroxisome biogenesis pathways were remarkably enhanced, and the genes AOX1 and AOX2 were upregulated higher than 30-fold, which was consistent with the experimental results of AOX expression. The Mig proteins had a slight effect on autophagy when cells were grown in glycerol. The expression analysis of transcription factors showed that deletion of MIG1 and MIG2 significantly upregulated the binding of an essential transcription activator, Mit1p, with the AOX1 promoter, which suggested that Mig proteins might regulate the AOX1 promoter through the regulation of Mit1p. This work provides a reference for the further exploration of the methanol induction and catabolite repression mechanisms of AOX expression in methylotrophic yeasts.
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Optimized inverse distance weighted interpolation algorithm for γ radiation field reconstruction
Zhang Biao,Cao Jinjia,Lin Shuang,Li Xiaomeng,Zhang Yulong,Zheng Xiaochang,Chen Wei,Song Yingming 한국원자력학회 2024 Nuclear Engineering and Technology Vol.56 No.1
The inversion of radiation field distribution is of great significance in the decommissioning sites of nuclear facilities. However, the radiation fields often contain multiple mixtures of radionuclides, making the inversion extremely difficult and posing a huge challenge. Many radiation field reconstruction methods, such as Kriging algorithm and neural network, can not solve this problem perfectly. To address this issue, this paper proposes an optimized inverse distance weighted (IDW) interpolation algorithm for reconstructing the gamma radiation field. The algorithm corrects the difference between the experimental and simulated scenarios, and the data is preprocessed with normalization to improve accuracy. The experiment involves setting up gamma radiation fields of three Co-60 radioactive sources and verifying them by using the optimized IDW algorithm. The results show that the mean absolute percentage error (MAPE) of the reconstruction result obtained by using the optimized IDW algorithm is 16.0%, which is significantly better than the results obtained by using the Kriging method. Importantly, the optimized IDW algorithm is suitable for radiation scenarios with multiple radioactive sources, providing an effective method for obtaining radiation field distribution in nuclear facility decommissioning engineering.
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Aerodynamic and acoustic behavior of a finite span wing equipped with synthetic jet actuator
Mohammad Moshfeghi,Hanru Liu,Yonghui Xie,Jinjia Wei,Nahmkeon Hur 대한기계학회 2013 대한기계학회 춘추학술대회 Vol.2013 No.12
In the current paper, aerodynamic and acoustic behaviors of a finite span wing with a synthetic jet actuator (SJA) are investigated using the DES turbulence model at three different angles of attack (AOA=14°, 18° and 25°). Aerodynamically, the main role of the SJA actuator is causing the vortex breakdown, weakening the separation effects and generating aerodynamic forces which fluctuate in time. As the AOA increases, the separated area becomes larger and stronger and, hence, the lift and drag values turns from the harmonic shapes to irregular fluctuation. From the acoustic point of view, for the AOAs of 14° and 18° the sound source occurs near the trailing edge. However, at AOA=25°, the dominant frequency of sound spectrum tends to move to a low frequency and the overall sound pressure level becomes the highest. Also, for this AOA the sound emits from both trailing edge and leading edge. Moreover, it is observed that the sound directivity changes to a no-dipole shape at a frequency lower than that of AOA=14° and 18°.
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