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( Jing Wu ),( Xin Yu Tan ),( Xiao Zhong Peng ),( Jian Gang Yuan ),( Bo Qin Qiang ) 생화학분자생물학회 2003 BMB Reports Vol.36 No.4
For better understanding of functions of the Calcyclin Binding Protein (CacyBP) and exploring its possible roles in neuronal differentiation, the subcellular localization of human CacyBP was examined in retinoic acid(RA)-induced and uninduced neuroblastoma SH-SY5Y cells. Immunostaining indicated that CacyBP was present in the cytoplasm of uninduced SH-SY5Y cells, in which the resting Ca^(2+) concentration was relatively lower than that of RA-induced cells. After the RA induction, immunostaining was seen in both the nucleus and cytoplasm. In the RA-induced differentiated SH-SYSY cells, CacyBP was phosphorylated on serine residue(s), while it existed in a dephosphorylated form in normal (uninduced) cells. Thus, the phosphorylation of CacyBP occurs when it is translocated to the nuclear region. The translocation of CacyBP during the RA-induced differentiation of SH-SY5Y cells suggested that this protein might play a role in neuronal differentiation.
High-Speed Cam Design for Quick-Action Linkage System
Jing Wu,Rui-Jun Yan,Chao Yuan,Yong Seok Lee,Kyoo-Sik Shin,Chang-Soo Han 제어로봇시스템학회 2014 제어로봇시스템학회 국제학술대회 논문집 Vol.2014 No.10
As a specially designed mechanism, cam is usually be used in many high-speed structures and mechanisms. Diverse geometric cam counters support for diverse motion applications, including the displacement, the velocity and the acceleration. This paper proposes a design of a high-speed cam which can be applied in a quick-action-based four bar linkage system. This variable high-speed cam is design by using the graphical combination of 5<SUP>th</SUP> and 7<SUP>th</SUP> polynomial cam curves. In addition, the kinematic model of a linkage system based on our proposed cam is constructed. Finally, the simulation results of the cam counter, velocity and acceleration of the designed cam are shown to validate the appropriate application of a quick-action linkage system.
Calculation on the Ion Flow Field under HVDC Transmission Lines Considering Wind Effects
Jing Wu,Sheng Gao,Yuxiao Liu 대한전기학회 2015 Journal of Electrical Engineering & Technology Vol.10 No.5
Based on Deutsch assumption, a calculation method on the electric field over the ground surface under HVDC transmission lines in the wind is proposed. Analyzing the wind effects on the electric field and the space charge density the existing method based on Deutsch assumption is improved through adding the wind speed to the ion flow field equations. The programming details are illustrated. The calculation results at zero wind speed are compared with available data to validate the code program. Then the ionized fields which resulted from corona of ±800kV HVDC lines are analyzed. Both the electric field and the current density on the ground level are computed under different wind direction and speed. The computation results are in good agreement with measurements. The presented method and code program can be used to rapidly predict and evaluate the wind effects in HVDC transmission engineering.
Reduced Graphene Oxide-Based Assay for Real-Time Monitoring of Cancer Cell Viability
Jing Wu,Yang Tang,Zengliang Shi,Zhengshan Tian,Junhua Wang,Chi Yang,Jitao Li,Zhongdang Xiao,Chunxiang Xu 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2015 NANO Vol.10 No.7
Viability of cancer cell is an important indicator of physiological state and function of cells, which can be effected by the change of pH in the medium solution, due to the increase of carbon oxide and lactic acid caused by respiration. Although many methods have been developed to detect the viability of cells, mostly based on cytochemical staining and polymerase chain reaction (PCR) technology are time consuming. In this paper, an electronic device was made by thermal reduced graphene oxide (RGO) for detection of cancer cell viability in real-time. This electronic device could be used to monitor the metabolic activity and viability of cancer cells based on the change in pH value. As the pH decreases, colon cancer cells loose viability and the current decreases. This RGO device is simple, sensitive and label-free and could serve as a platform for detection of cells and drug testing.
Jing Wu,Xintong Wang,Li Wu,Ya-ni Lu,Yanhua Han 대한토목학회 2022 KSCE Journal of Civil Engineering Vol.26 No.8
As tunnelling progresses into the complex geological environment such as fault zones, water inrush has become one of the main geological hazards during tunnel construction. Consequently, understanding the evolution of pore pressure and flow velocity when a tunnel is excavated in a fault zone is crucial to ensure safe working conditions and reduce construction risks. In this work, based on the concept of “Three Zones” fault structure, we simulate the nonlinear water inrush process by solving the Darcy-Brinkman flow equation for the host rock and the fault zone. We examine the impacts of 1) the angle between the tunnelling direction and the fault and 2) the relative position from the tunnel face to the fault on the evolution of pore pressure and flow velocity near the tunnel face. The results show that within 5 m to 20 m ahead of the working face, pore pressure, flow velocity, and water inrush rate are the smallest when the angle is 90°. As the angle decreases, both pore pressure and flow velocity ahead of the working face increase. The pore pressure is larger when the tunnel has not reached the fault zone than when the tunnel has crossed the fault zone. Flow velocity also exhibits similar behaviour as pore pressure. With different relative positions from the tunnel working face to the fault, the closer the tunnel face to the fault, the lower the pore pressure and the larger the flow velocity ahead of the tunnel face. The largest water inrush rate occurs when the tunnel face is excavated to the center of the fault core, and the water inrush rate declines as the distance away from the fault increases. The simulation results provided a new method for simulating water inrush when a tunnel crosses a fault and could provide valuable references for the prediction of water inrush for underground projects.
Keratin 1 maintains the intestinal barrier in ulcerative colitis
Jing Wu,Junkun Niu,Maojuan Li,Yinglei Miao 한국유전학회 2021 Genes & Genomics Vol.43 No.12
Background The intestinal mechanical barrier plays a key role in the pathogenesis of ulcerative colitis (UC). Our previous study showed keratin 1 (KRT1) was downregulated in UC, but the mechanism by which KRT1 afects the intestinal barrier remains unknown. Objectives To explore the mechanism of KRT1 in the intestinal barrier in UC. Methods Colonic tissues were collected from 20 UC patients before and after mucosal healing (MH) and 15 healthy controls. The expression of KRT1 was measured by PCR, western blotting and immunohistochemistry (IHC). A dextran sulfate sodium (DSS)-induced colitis model was established in krt1 transgenic (TG) mice, and the mice were treated with methylprednisolone (MP) to explore the role of KRT1 in the intestinal barrier. Infammation was evaluated through the DAI score, colon, spleen and H&E. The expression of KRT1 and tight junction (TJ) proteins in mouse was analysed by the same methods. Results The transcription and expression of KRT1 in UC was decreased and recovered after MH but did not reach the level of the healthy controls. Similar to the clinical results, the expression of krt1 was decreased in DSS-induced colitis and upregulated after MP. Moreover, the krt1 TG group exhibited less infammation than wild-type (WT) group. The expression of Occludin and ZO-1 decreased after DSS induction, the decreases in Occludin and ZO-1 in the krt1 TG group were lower than WT group, which was signifcantly increased after MP, while the expression of Claudin-2 exhibited the opposite efect. Conclusions Keratin 1 maintains the intestinal barrier by upregulating TJ proteins in UC.