Prediction of Exhaust Contaminant of Gasoline Vehicles Based on Grey Model GM (1,1) and Artificial Neural Networks

Jingbin Song,Shuzhao Li 보안공학연구지원센터 2015 International Journal of Hybrid Information Techno Vol.8 No.11

Exhaust contaminant of gasoline vehicles is a crucial aspect to measure the vehicle performances and the air pollutions. According to the feature of vehicles, the emission of exhaust contamination of a vehicle is different as time goes by, which shows an increase tendency in most of the cases. Measuring the changes of a vehicle's exhaust contaminant emission is of great importance in the field of vehicle engineering. However, it is hard to determine and find out the regulations of the emission, needing a long time for regular determination and advanced relevant machines. In this article, we aim at providing two novel methods for the prediction of exhaust contaminant of gasoline vehicles, using grey model GM (1,1) and artificial neural networks (ANNs) models respectively. Results show that both the GM (1,1) model and ANN models are comparatively precise for the prediction. The GM (1,1) model can quickly obtain the predicted values of exhaust contaminant, but it is less precise than ANN models. However, ANN models need more time for the training process, compared to GM (1,1) mo

Recent Development of Laboratory-made Solid-phase Microextraction Fibers on the Application of Food Safety Analysis

Jingbin Zeng,Jinmei Chen,Wenfeng Chen,Xiaoli Huang,Liangbi Chen,Xi Chen 한국식품과학회 2009 Food Science and Biotechnology Vol.18 No.3

Solid-phase microextraction (SPME) has gained widespread acceptance in sample pretreatment due to its solventfree and easy-to-operate properties. SPME fibers are considered as a key part of SPME technique, since it primarily determines the extraction performance of the method including sensitivity, selectivity, and reproducibility. Generally speaking, target analyte with different chemical property requires fiber coating that has the best affinity towards it. Due to the lack of varieties of commercial fibers available currently, considerable efforts have been recently made to develop tailor-made fibers to fulfill increasing demands of different analysis. This paper concisely classify some SPME fiber preparation approaches such as solgel technology, physical deposition, molecularly imprinted technique, and their respective application in food safety analysis.

RUNX1 Upregulation Causes Mitochondrial Dysfunction via Regulating the PI3K-Akt Pathway in iPSC from Patients with Down Syndrome

Jingbin Yan,Yanna Liu,Yuehua Zhang,Zhaorui Ren,Fanyi Zeng 한국분자세포생물학회 2023 Molecules and cells Vol.46 No.4

Down syndrome (DS) is the most common autosomal aneuploidy caused by trisomy of chromosome 21. Previous studies demonstrated that DS affected mitochondrial functions, which may be associated with the abnormal development of the nervous system in patients with DS. Runt-related transcription factor 1 (RUNX1) is an encoding gene located on chromosome 21. It has been reported that RUNX1 may affect cell apoptosis via the mitochondrial pathway. The present study investigated whether RUNX1 plays a critical role in mitochondrial dysfunction in DS and explored the mechanism by which RUNX1 affects mitochondrial functions. Expression of RUNX1 was detected in induced pluripotent stem cells of patients with DS (DS-iPSCs) and normal iPSCs (N-iPSCs), and the mitochondrial functions were investigated in the current study. Subsequently, RUNX1 was overexpressed in N-iPSCs and inhibited in DS-iPSCs. The mitochondrial functions were investigated thoroughly, including reactive oxygen species levels, mitochondrial membrane potential, ATP content, and lysosomal activity. Finally, RNA-sequencing was used to explore the global expression pattern. It was observed that the expression levels of RUNX1 in DS-iPSCs were significantly higher than those in normal controls. Impaired mitochondrial functions were observed in DS-iPSCs. Of note, overexpression of RUNX1 in N-iPSCs resulted in mitochondrial dysfunction, while inhibition of RUNX1 expression could improve the mitochondrial function in DS-iPSCs. Global gene expression analysis indicated that overexpression of RUNX1 may promote the induction of apoptosis in DS-iPSCs by activating the PI3K/Akt signaling pathway. The present findings indicate that abnormal expression of RUNX1 may play a critical role in mitochondrial dysfunction in DS-iPSCs.

NUMERICAL STUDY ON CAVITATION FLOW CHARACTERISTICS IN DIESEL FUEL INJECTOR CONTROL VALVE

Zhenming Liu,Jingbin Liu,Jiechang Wu,Xiaolei Gu 한국자동차공학회 2022 International journal of automotive technology Vol.23 No.4

The flow and cavitation in the flow passage of the control valve are calculated, and the effects of operating conditions (including injection pulse width, rail pressure and ball valve lift) on the flow and cavitation characteristics are analyzed under dynamic boundary conditions. The simulation results show that the cavitation in the outflowing control-orifice (OA) and the guide-hole are almost unaffected by these operating conditions. In contrast, the cavitation process in the ball valve chamber has two distinct stages, which can be classified as violent and relatively smooth. The shorter the pulse width, the more severe the average degree of cavitation in the ball valve chamber; however, the risk of cavitation erosion on the ball valve and the ball valve seat surface does not increase too much. The increase of rail pressure and the increase of ball valve lift will aggravate the cavitation, and the cavitation position will move forward closer to the sealing annular surface.

ROCK1 induces dopaminergic nerve cell apoptosis via the activation of Drp1-mediated aberrant mitochondrial fission in Parkinson’s disease

Qian Zhang,Changpeng Hu,Jingbin Huang,Wuyi Liu,Wenjing Lai,Faning Leng,Qin Tang,Yali Liu,Qing Wang,Min Zhou,Fangfang Sheng,Guobing Li,Rong Zhang 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-

Dopamine deficiency is mainly caused by apoptosis of dopaminergic nerve cells in the substantia nigra of themidbrain and the striatum and is an important pathologic basis of Parkinson’s disease (PD). Recent research has shownthat dynamin-related protein 1 (Drp1)-mediated aberrant mitochondrial fission plays a crucial role in dopaminergicnerve cell apoptosis. However, the upstream regulatory mechanism remains unclear. Our study showed that Drp1knockdown inhibited aberrant mitochondrial fission and apoptosis. Importantly, we found that ROCK1 was activated inan MPP+-induced PD cell model and that ROCK1 knockdown and the specific ROCK1 activation inhibitor Y-27632blocked Drp1-mediated aberrant mitochondrial fission and apoptosis of dopaminergic nerve cells by suppressing Drp1dephosphorylation/activation. Our in vivo study confirmed that Y-27632 significantly improved symptoms in a PDmouse model by inhibiting Drp1-mediated aberrant mitochondrial fission and apoptosis. Collectively, our findingssuggest an important molecular mechanism of PD pathogenesis involving ROCK1-regulated dopaminergic nerve cellapoptosis via the activation of Drp1-induced aberrant mitochondrial fission.

A compression strategy to accelerate LSTM meta-learning on FPGA

NianYi Wang,Jing Nie,JingBin Li,Kang Wang,ShunKang Ling 한국통신학회 2022 ICT Express Vol.8 No.3

Driven by edge computing, how to efficiently deploy the meta-learner LSTM in the resource constrained FPGA terminal equipment has become a big problem. This paper proposes a compression strategy based on LSTM meta-learning model, which combined the structured pruning of the weight matrix and the mixed precision quantization. The weight matrix was pruned into a sparse matrix, then the weight was quantified to reduce resource consumption. Finally, a LSTM meta-learning accelerator was designed based on the idea of hardware–software cooperation. Experiments show that compared with mainstream hardware platforms, the proposed accelerator achieves at least 50.14 times increase in energy efficiency.

FK866 inhibits colorectal cancer metastasis by reducing NAD+ levels in cancer-associated fibroblasts

Xie Hanhan,Lei Yun,Mao Yushan,Lan Jingbin,Yang Jing,Quan Hui,Zhang Tao 한국유전학회 2022 Genes & Genomics Vol.44 No.12

Background: Extraintestinal metastasis is the main therapeutic challenge for colorectal cancer, the third most common cancer worldwide. Various components of the tumor microenvironment, especially cancer-associated fibroblasts (CAFs), play important roles in tumor metastasis. NAMPT is often overexpressed in tumor tissues and is associated with poorer prognosis. However, the specific roles of NAMPT as well as NAD+ in tumor metastasis are relatively unknown. Therefore, we investigated the role of NAMPT and related NAD+ metabolism in cancer-associated fibroblasts mediated colorectal cancer metastasis. Objective: This study sought to explore the molecular mechanism of FK866 in CAFs cell and colorectal cancer proliferation and metastasis. Methods: The expression of NAMPT in clinical tissues were detected by immunohistochemically analysis. To investigate the role of NAMPT and NAD+ in the interactions between cancer cells and cancer-associated fibroblasts in tumor microenvironment, we isolated CAFs from normal and cancer tissues of clinical colorectal cancer patients. CAFs were treated with different concentrations of FK866, inhibitor of NAMPT, then the NAD+ content was detected using kits, the expression of CAFs activity and stemness indexes was assessed by Western blot and immunofluorescence. The secreted factors of these cells were analyzed by cellular inflammatory factor microarrays. The migration of SW480 after co-cultured with FK866-treated CAFs was detected by Transwell. Finally, high-throughput sequencing was performed to identify the proteins that are associated with the effect of altered NAD+ in CAFs on the migration of cancer cells. Results: NAMPT expression is significantly higher in colorectal cancer tissues, especially in metastatic cancer patients, than that in normal tissues. Inhibition of NAMPT by FK866 in CAFs decreases the expression of activity indicators (α-SMA, PDGFRβ), stemness indicators (BMI-1, OCT4), inflammatory factors and chemokines. Meanwhile, FK866 treatment inhibits the migration ability of SW480 cells co-cultured with CAFs. Finally, high-throughput sequencing reveals that PITX3 are down-regulated after NAD+ reduction in CAFs, which could be reversed by adding NAM, a raw material for NAD+ synthesis. Conclusion: Inhibition of the NAMPT-mediated NAD+ synthesis by FK866 may decrease the activation and stemness of CAFs, reduce the secretion of inflammatory and chemokines by suppressing the expression of PITX3, resulting in the suppression of colorectal cancer metastasis.