mRNA Expression of Ovine Angiopoietin-like Protein 4 Gene in Adipose Tissues

Zhang, Jing,Jing, Jiong-Jie,Jia, Xia-Li,Qiao, Li-Ying,Liu, Jian-Hua,Liang, Chen,Liu, Wen-Zhong Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.5

Angiopoietin-like protein 4 (ANGPTL4) is involved in a variety of functions, including lipoprotein metabolism and angiogenesis. To reveal the role of ANGPTL4 in fat metabolism of sheep, ovine ANGPTL4 mRNA expression was analyzed in seven adipose tissues from two breeds with distinct tail types. Forty-eight animals with the gender ratio of 1:1 for both Guangling Large Tailed (GLT) and Small Tailed Han (STH) sheep were slaughtered at 2, 4, 6, 8, 10, and 12 months of age, respectively. Adipose tissues were collected from greater and lesser omental, subcutaneous, retroperitoneal, perirenal, mesenteric, and tail fats. Ontogenetic mRNA expression of ANGPTL4 in these adipose tissues from GTL and STH was studied by quantitative real time polymerase chain reaction. The results showed that ANGPTL4 mRNA expressed in all adipose tissues studied with the highest in subcutaneous and the lowest in mesenteric fat depots. Months of age, tissue and breed are the main factors that significantly influence the mRNA expression. These results provide new insights into ovine ANGPTL4 gene expression and clues for its function mechanism.

Up-regulation of Thy-1 Promotes Invasion and Metastasis of Hepatocarcinomas

Cheng, Bian-Qiao,Jiang, Yi,Li, Dong-Liang,Fan, Jing-Jing,Ma, Ming Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.4

Increasing evidence has revealed that thy-1 was a potential stem cell marker of liver cancer, but no data have been shown on how thy-1 regulates the pathophysiology of liver cancer, such as proliferation, apoptosis, invasion and migration. We previously demonstrated that thy-1 was expressed in about 1% of hepg2 cells, thy-1+hepg2 cells, but not thy-1-, demonstrating high tumorigenesis on inoculation $0.5{\times}10^5$ cells per BACA/LA mouse after 2 months. In the present study, our results showed that higher expression of thy-1 occurs in 72% (36/50 cases) of neoplastic hepatic tissues as compared to 40% (20/50 cases) of control tissues, and the expression of thy-1 is higher in poorly differentiated liver tumors than in the well-differentiated ones. In addition, thy-1 expression was detected in 85% of blood samples from liver cancer patients, but none in normal subjects or patients with cirrhosis or hepatitis. There was a significant negative correlation between thy-1expression and E-cadherin expression (a marker of invasion and migraton), but not between thy-1 expression and AFP expression in all the liver cancer and blood samples. We further investigated the relationship between thy-1 and E-cadherin in liver cancer hepg2 cell line which was transfected with pReceiver-M29/thy-1 eukaryotic expression vector followed by aspirin treatment. Lower expression of E-cadherin but higher expressions of thy-1 were detected in hepg2 cells transfected with pReceiver-M29/thy-1. Taken together, our study suggested that thy-1 probably regulates liver cancer invasion and migration.

Ceratovacuna atrovirensa Qiao, a new species from China (Aphididae: Hormaphdinae)

Jiang Li-Yun,Chen Jing,Xu Ying,Qiao Ge-xia 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.3

Ceratovacuna atrovirensa Qiao sp.n., a woolly aphid on Indocalamus tessellatus from China, is described and illustrated. The new species is supported by both morphological and molecular data. An updated key to apterous viviparous females of Ceratovacuna is provided. The type specimens are deposited in the National Zoological Museum of China, Institute of Zoology, the Chinese Academy of Sciences, Beijing, China and the Natural History Museum, London, United Kingdom.

Clinical and Pathologic Features of Multifocal and Multicentric Breast Cancer in Chinese Women: A Retrospective Cohort Study

Mei-rong Zhou,Zhong-hua Tang,Jing Li,Jin-Hu Fan,Yi Pang,Hong-jian Yang,Shan Zheng,Jing-qiao Bai,Ning Lv,You-Lin Qiao,Feng Xu,Hai-zhi Qi 한국유방암학회 2013 Journal of breast cancer Vol.16 No.1

Purpose: This study aims to analyze the clinical-pathological characteristics of multifocal and multicentric breast cancer (MMBC) in Chinese women. Methods: Sixty-seven cases with MMBC were randomly collected and reviewed at seven hospitals in representative districts of China during 1999 to 2008. Results: The incidence of MMBC in breast cancer in China was 1.75%. Compared to those with unifocal breast cancer, women with MMBC were more likely to have larger tumor size, lymph node metastasis (59.70% vs. 45.62%) and stage III to IV (46.26% vs. 21.10%). The peak age at onset of MMBC was 40 to 49 years old and has been gradually increasing during 1999 to 2008. Most of the MMBC women were treated with surgery and adjuvant therapy. Conclusion: In China, the incidence of MMBC in breast cancer is significantly lower than that in Western countries. Compared to unifocal breast cancer, MMBC is biologically more aggressive. Most MMBC women underwent mastectomy, instead of breast conservation surgery.

Genetic variation and population structure of the oriental fruit moth Grapholita molesta in Shanxi, a major pome fruits growing region in North China

Jing Yang,Zhong-Fang Liu,Ji-Qiao Fan,Yu-PengWu,Rui-Yan Ma,Ren-Jun Fan 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.4

Grapholitamolesta, commonly known as the oriental fruit moth, is one of the most destructive fruit pests both in China and in other regions of the world. We examined the genetic diversity and population structures of G. molesta populations from 11 locations in a major pome fruit production region (Shanxi province) in North China using seven microsatellite markers and sequence variations in two mitochondrial genes. We found that G.molesta in the studied region exhibited high levels of genetic diversity. Among 271 individual insects examined using microsatellite markers, mean number of alleles ranged from 5.86 to 9.00 with average 8.01. Population structure analysis revealed that populations from the eleven sampling sites formed two clusters without apparent distance isolation. Cluster 1 consisted of eight populations distributed in five regions of the sampling sites, whereas Cluster 2 consisted of three populations distributed in two distant locations of the sampling sites. Genetic exchange analysis revealed that about half of the haplotypes were shared among over 90% individual insects from different regions. Our results indicated that the population structure and genetic exchange of G. molesta in the studied region might have been affected by limited dispersal ability of the insect, environmental condition, colonization history and human-mediated dispersal. These findings should provide useful information for the implementation of area-wide pest management and for further characterization of genetic variation and population structure of this economically important pest.

Research on Oscillation Character of Six-Phase Fractional-Slot Concentrated-Winding Permanent Motor with Different Slot-Pole Match

Qiao Ming-zhong,Zhu Yong-xin,Liang Jing-hui,Li Geng 대한전기학회 2016 Journal of Electrical Engineering & Technology Vol.11 No.6

The oscillation character of permanent magnetic motor is highly related to its slot-pole match. By calculating air-gap magnet field and radial electromagnetic force of 6-phase fractional-slot concentrated-winding permanent magnetic motor with slot–pole match of 48/44,48/46,48/50 and 48/52 under no load and load status, oscillation character of permanent magnetic motor is analyzed. A 20kW prototype with 48 slots and 44 poles is designed. With many sensors attaching to the corresponding parts, oscillatory acceleration is measured, and spectrum of oscillation frequency is recorded as well. The experiment results give proof to the analysis method for permanent magnetic motor oscillation in this paper.

Effect of Different Preharvest and Postharvest Treatments on Glucoraphanin Content and Quinone Reductase Inducer Activity in Broccoli Florets

Qiao-Mei Wang,Chao-Jiong Xu,Jing Yuan,De-Ping Guo 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-

Development of Nested PCR, Multiplex PCR, and Loop-Mediated Isothermal Amplification Assays for Rapid Detection of Cylindrocladium scoparium on Eucalyptus

Qiao, Tian-Min,Zhang, Jing,Li, Shu-Jiang,Han, Shan,Zhu, Tian-Hui The Korean Society of Plant Pathology 2016 Plant Pathology Journal Vol.32 No.5

Eucalyptus dieback disease, caused by Cylindrocladium scoparium, has occurred in last few years in large Eucalyptus planting areas in China and other countries. Rapid, simple, and reliable diagnostic techniques are desired for the early detection of Eucalyptus dieback of C. scoparium prior to formulation of efficient control plan. For this purpose, three PCR-based methods of nested PCR, multiplex PCR, loop-mediated isothermal amplification (LAMP) were developed for detection of C. scoparium based on factor 1-alpha (tef1) and beta-tubulin gene in this study. All of the three methods showed highly specific to C. scoparium. The sensitivities of the nested PCR and LAMP were much higher than the multiplex PCR. The sensitivity of multiplex PCR was also higher than regular PCR. C. scoparium could be detected within 60 min from infected Eucalyptus plants by LAMP, while at least 2 h was needed by the rest two methods. Using different Eucalyptus tissues as samples for C. scoparium detection, all of the three PCR-based methods showed much better detection results than regular PCR. Base on the results from this study, we concluded that any of the three PCR-based methods could be used as diagnostic technology for the development of efficient strategies of Eucalyptus dieback disease control. Particularly, LAMP was the most practical method in field application because of its one-step and rapid reaction, simple operation, single-tube utilization, and simple visualization of amplification products.

Incidence and risk factors for venous thrombosis among patients with inflammatory bowel disease in China: a multicenter retrospective study

( Jing Liu ),( Xiang Gao ),( Ye Chen ),( Qiao Mei ),( Liangru Zhu ),( Jiaming Qian ),( Pinjin Hu ),( Qian Cao ) 대한장연구학회 2021 Intestinal Research Vol.19 No.3

Background/Aims: Risk of venous thrombosis is increased in patients with inflammatory bowel disease (IBD); data on Asian IBD patients is limited and status quo of thrombosis screening and prophylaxis are unknown. Therefore, we aimed to investigate the incidence, screening, prophylaxis, and risk factors for venous thrombosis among Asian IBD patients. Methods: Medical files of patients with Crohn’s disease (CD) and ulcerative colitis (UC) from 17 hospitals across China between 2011 and 2016 were reviewed for venous thrombosis, use of screening and prophylaxis. A case-control study was performed among hospitalized patients with venous thrombosis and their age-, sex-matched IBD controls hospitalized around the same period; disease characteristics and known provoking factors of venous thrombosis were recorded. Risk factors were analyzed in both univariate and logistic regression analyses. Results: A total of 8,459 IBD patients were followed for 12,373 person-year. Forty-six patients (0.54%) had venous thrombosis, yielding an incidence of 37.18 per 10,000 person-year. Incidence increased with age, especially among CD. Less than 20% of patients received screening tests and 35 patients (0.41%) received prophylaxis. Severe disease flare was an independent risk factor for venous thrombosis (odds ratio [95% confidence interval]: CD, 9.342 [1.813-48.137]; UC, 5.198 [1.268-21.305]); past use of steroids and extensive involvement were 2 additional risk factors in CD and UC, respectively. Conclusions: Incidence of venous thrombosis in China was 37.18 per 10,000 person-year (0.54%). Use of screening and prophylaxis were rare. Severe disease flare was an independent risk factor for thrombosis among hospitalized patients. (Intest Res 2021;19:313-322)

Research on the Development Strategies of Confucius Institute for Expanding Chinas Foreign Trade

Qiao Yanni,Yao Jinge,Ki-Hyung Bae 한국콘텐츠학회 2021 International Journal of Contents Vol.17 No.3

The purpose of this paper is to explore how the Confucius Institute Chinese international promotion could better promote the development of Chinas foreign trade, by analyzing the distribution of the Confucius Institute worldwide, based on the theory of language economics, using SWOT analysis to analyze the advantages and disadvantages of the internal environment, opportunities and challenges of the external environment of Chinese international promotion of Confucius Institute. The following findings were gathered: as a language teaching institution and information exchange platform, Confucius Institute has the ability to share trade information and increase trade opportunities; to improve cultural identity and reduce transaction costs; to promote cultural communication and integration, and drive the development of related industries. The internal disadvantages were mainly reflected in the mismatch between the global regional distribution structure of Confucius Institutes, and the economic and trade structure, such as, the asymmetry between language, culture output, and demand. In addition, the management mechanism was not perfect. External opportunities were mainly new opportunities brought by economic globalization, cultural diversity, and the development of the Belt and Road initiative. External challenges were mainly influenced by the China threat theory and the fierce cultural competition among countries. The corresponding countermeasures were put forward based on the advantages of the platform and grasping the external opportunities: improving the quality of operation and speeding up the localization process; respecting cultural differences and realizing cultural common learning; seeking multilateral cooperation and enhancing the capacity for independent development.