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Zhi‐Jun Zhou,Xiao-Yan KOU,Lei-YangQIAN,Jing LIU 한국곤충학회 2016 Entomological Research Vol.46 No.3
The Chinese bush cricket, Gampsocleis gratiosa, has a long history as a pet in China. To date, the sequencing of its whole genome is unavailable as a non‐model organism. Transcriptomic information is also scarce for this species. The G. gratiosa transcriptome was sequenced using Illumina HiSeq 2000 paired‐end sequencing technology. In total 52 million clean reads with an average length of 90 bp were generated, which produced 74,821 unigenes with a mean length of 580 bp and an N50 length of 759 bp. In total 29,674 (39.66%) unigenes were successfully annotated against the NR, NT, Swiss‐Prot, Gene Ontology (GO), Clusters of Orthologous Groups of proteins (COGs) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. Further functionally classified of unigenes against GO, COGs and KEGG found that a total of 11,935 (19.95%) unigenes were categorized into 61 GO terms, 19,576 unigenes were clustered into 25 COG functional categories and 17,971 unigenes were assigned to 258 KEGG pathways. In addition, 2093 microsatellite loci were identified, of which 591 loci had flanking sequences suitable for polymerase chain reaction (PCR) primer design. The transcriptome profile of G. gratiosa contributed to the accumulation of orthopteran genomic data, and the microsatellite loci provided useful tools for future studies of this and other closely related Gampsocleis species.
Zhi-Fu Guo,Li-Jun Zhang,Ming Zhong,Yu-Ming Wei,Li Zhang,Hui Ma,Hao-Ge Li,Li-Jing Chen,Jing-Wei Lin,You-Liang Zheng 한국유전학회 2010 Genes & Genomics Vol.32 No.3
By acid polyacrylamide gel electrophoresis (A-PAGE) analysis,it was indicated that the electrophoresis mobility of gliadins from Crithopsis delileana (Schult) Roshev (2n=2x=14,KK) had obvious difference with those from common wheat in α, γ and ω region. Using homologous primers, two γ-gliadin genes (gli-Kr1 and gli-Kr2) were isolated from C. delileana,which had been deposited in the GenBank under accession numbers EU283818 and EU283821, respectively. Two γ-gliadin genes of C. delileana had the similar primary structures to the corresponding gene sequences from other wheat related species. The differences were mainly resulted from substitutions,insertions and deletions involving single amino acid residues or motifs of γ-gliadins. The repetitive domains of gli-Kr1 and gli-Kr2 from C. delileana are shorter than most of other sequences. By the alignment of γ-gliadin genes from A, B, D, Am, Au, S, Sl, Ssh, Ss and Sb genomes of Triticum and Aegilops, R genome of Secale (γ-secalin), Ee genome of Lophopyrum and K genome of Crithopsis in Triticeae, phylogenetic analysis indicated that two γ-gliadin genes of C. delileana could be clustered together with a γ-gliadin genefrom Ssh genome of Aegilops by an interior paralleled branch. It was the first time that the γ-gliadin genes encoded by K genome of C. delileana were characterized. These could offer precious information for better understanding the qualities associated with gliadins, the response in coeliac disease and studying the evolutionary relationship of gliadins in Triticeae.
Decoding Process of RS Codes with Errors and Erasures: An Overview
Zhi Jing,최효정,송홍엽 한국통신학회 2022 韓國通信學會論文誌 Vol.47 No.11
This paper provides a comprehensive overview of the hard-decision decoding process of Reed-Solomon codes for error-and-erasure decoding. For an [n, k] RS code, the decoder can correct simultaneously v errors and m erasures in the received data if 2v + m ≤n - k (correctable range) and will fail otherwise (uncorrectable range). We give detailed reviews of both Berlekamp-Massy and Continued-Fraction algorithms for error-and-erasure decoding. Berlekamp-Massy algorithm has long been known but sometimes appeared incorrectly in some references. Continued-Fraction algorithm has been recently applied for error-and-erasure decoding. Finally, we verify by simulation that two algorithms work exactly the same even in the uncorrectable range.