Combined transcriptomic and proteomic analysis of flubendiamide resistance in Plutella xylostella

Li Jing‐Jing,Jin Ming‐Hui,Wang Nian‐Meng,Yu Qi‐Tong,Shang Ze‐Yu,Xue Chao‐Bin 한국곤충학회 2020 Entomological Research Vol.50 No.10

Diamondback moth (DBM), Plutella xylostella, is an important pest of crucifers worldwide. The extensive use of diamide insecticides has led to DBM resistance in the world, and this presents a serious threat to vegetable production. In the present study, transcriptomic and proteomic analyses were combined to investigate the potential flubendiamide‐resistance mechanism in DBM. The lab‐selected (Rh) and field‐collected (Rb) flubendiamide‐resistant lines of P. xylostella with resistance ratios of 1889.92‐fold and 1250.97‐fold, respectively, were used, as well as a lab‐reared flubendiamide‐susceptible line (S). Compared with the S group, the transcriptomic analysis revealed 151 upregulated and 287 downregulated gene messengers in the Rh group and 432 upregulated and 565 downregulated gene messengers in the Rb group. The most frequently enriched pathways of differentially expressed genes (DEGs) were mainly involved in metabolic pathways. Metabolism related genes, including two P450, two ABC transporters, and three trypsins, were upregulated in the Rh line. Additionally, some P450 genes, trypsin, juvenile hormone (JH), and mucin genes were also upregulated in the Rb line. In proteomic analysis comparisons with the S group, there were 78 upregulated and 90 downregulated proteins in the Rh group and 221 upregulated and 155 downregulated proteins in the Rb group. Further analyses found that three CYP and 11 CYP proteins were over‐expressed in Rh and Rb lines, respectively. Four glutathione S‐transferase (GST) and four UGTs were over‐expressed in Rb line. So, we deduced that the detoxification metabolism may be the main mechanism of flubendiamide resistance in P. xylostella.

朝鮮時期『虞註杜律』的傳入和翻印版本硏究

劉婧 ( Liu Jing ) 중국어문학회 2022 中國語文學誌 Vol.- No.81

WuZhuDuLv(虞註杜律) is a compilation of Du Fu(杜甫)’s Poems which were edited by Yu Ji(虞集) during the Yuan(元) Dynasty of China. WuZhuDuLv(虞註杜律) was first printed by Zhu Xiong(朱熊) of Xuande(宣德, 1434) in the Ming Dynasty, and re-printed by Lin Jing(林靖) during the period of Zhengtong(正統, 1443). Lin Jing’s printed edition was first introduced to Choseon during the period of Chenghua(成化). Over the next four hundred years, WuZhuDuLv(虞註杜律) was reprinted and published several times as a teaching material for the study of Du Fu(杜甫)’s poetry and other Chinese poetry, which had a great influence in Choseon, The book, WuZhuDuLv(虞註杜律) introduced into Choseon, first reprinted by QingzhouMu(淸州牧). It was a book printed from a wooden block. Later reproduced the metal types of Jiayin(甲寅) and Yunge(芸阁). There are many types of reprinted editions and there are also differences between the first and later editions. In the late Choseon, woodblock printed books of the WuZhuDuLv(虞註杜律) were printed the most. This study summarized and analyzed various editions in which the WuZhuDuLv(虞註杜律) was introduced into Choseon from the literature’s perspective. Through this fact, it is possible to understand the various situations in which Du Fu’s poems related literature was printed and distributed in Choseon. And it will be great help to study of publication and dissemination of Du Fu’s poems in the Choseon Dynasty.

Up‑regulation of miR‑27 extenuates lipopolysaccharide‑induced injury in H9c2 cells via modulating ICAM1 expression

Jing‑Fang Xiang,Jian‑Chun Yu,Jian‑You Zhu 한국유전학회 2019 Genes & Genomics Vol.41 No.12

Background MiR-27 has been found to present an overt myocardial expression during cardiogenesis. However, whether miR-27 involves in myocarditis development and the possible molecular mechanism remain unknown. The purpose of this study was to investigate the biological characteristic of miR-27 in LPS-damaged H9c2 cells. Methods H9c2 cells were treated with lipopolysaccharide (LPS, 10 μg/ml) for 12 h to form cell injury. MiR-27 mimic and inhibitor were used to up-regulate or down-regulate miR-27 expression. MTT assay and flow cytometry analysis were conducted to test cell viability and apoptosis. The relative RNA expression level of miR-27 and intercellular adhesion molecule 1 (ICAM1) was determined by qRT-PCR. Luciferase reporter gene assay was utilized to confirm the interaction between miR-27 and ICAM1. Western blot was used to determine the protein expression levels. Results We observed that LPS treatment significantly decreased the level of miR-27 in H9c2 cells. Moreover, LPS exposure suppressed cell viability, promoted cell apoptosis and increased the relative expression of p-NF-κB p65/NF-κB p65 and p-IκBα/IκBα. Up-regulation of miR-27 increased cell proliferation and reduced cell apoptosis, while down-regulation of miR-27 suppressed cell growth and promoted cell apoptosis. ICAM1 was predicted and verified as a target of miR-27, and the expression of ICAM1 is negatively regulated by miR-27. The relative expression of p-NF-κB p65/NF-κB p65 and p-IκBα/ IκBα was dramatically decreased by miR-27 mimic and increased by miR-27 inhibitor. Conclusion Our study illustrated that up-regulation of miR-27 exhibits a protective effect on LPS-damaged H9c2 cells, which may be achieved by regulating ICAM1 and NF-κB signaling.

치유환경디자인 연구 경향 -국내 선행 연구 분석을 중심으로-

담정 ( Tan¸ Jing ),신윤진 ( Shin¸ Yoon Jhin ) 한국기초조형학회 2021 기초조형학연구 Vol.22 No.4

현대인들은 건강에 점점 더 관심을 가지게 되면서 건강 회복을 돕는 치유환경도 주목을 받게 되었다. 특히 치유환경디자인 연구가 증가하는 추세다. 따라서 본 연구는 치유환경디자인 연구 경향을 살펴보기 위해 2003년부터 2020년 6월까지 한국 국내 석·박사학위논문과 국내 학술지(KCI)논문 중 치유환경디자인에 관한 논문 127편을 선정하여 데이터를 분석하였다. 연구는 치유환경의 개념과 요소 분석, 논문 개수, 연구 주제, 연구 방법, 연구 내용, 연구 범위, 연구 대상 등의 내용이 포함된다. 분석 결과를 정리하면 아래와 같다. 첫째, 치유환경디자인은 이용자의 관점에서 치유환경과 소통하는 매개체다. 즉 환경디자인을 통해 치유효과를 높이는 것이다. 치유환경은 신체적, 심리적, 사회적 요소를 포함한다. 둘째, 논문 개수는 해마다 증가하는 모습을 보여준다. 특히 2012년에는 거의 배로 급증하였다. 셋째, 초기에는 의료기관, 요양원 등과 관련된 연구가 대부분 이었으나 후반부에는 의료기관 이외의 다양한 공간에 대한 연구 논문이 증가하며, 이후 상승과 하락을 반복한다. 넷째, 사례분석에 기반한 연구 방법이 전 단계에 걸쳐 우위를 차지하고 있다. 다섯째, 연구 내용은 주로 치유환경 디자인의 건축적 공간 요소에 관한 것이다. 여섯째, 연구 범위는 병원을 중심으로 하며, 내부 공간 계획에 집중되어 있다. 일곱째, 연구 대상은 전기, 중기, 후기에 각각 다른 변화를 보인다. 치유환경디자인에 관한 연구는 과학적 근거에 기반하고, 심리적·사회적 요소에 관한 연구가 추가적으로 이루어져야 한다. As modern people become more and more interested in health, the healing environment, which helps them recover, has also drawn attention. In particular, research on healing environmental design is increasing. Therefore, this study selected 127 papers on healing environment design from 2003 to June 2020 among Korean master's and doctorate papers and Korean journal (KCI) papers to analyze the data. Research includes the concept and elemental analysis of the healing environment, the number of papers, research topics, research methods, research contents, scope of research, and subjects of research. The analysis results are as follows. First, healing environment design is a medium that communicates with the healing environment from the user's point of view. In other words, it enhances the healing effect through environmental design. The healing environment includes physical, psychological, and social factors. Second, the number of papers increases year by year. In particular, the number nearly doubled in 2012. Third, most of the research involved in medical institutions, nursing homes, etc. initially, but in the second half, research papers on a variety of spaces other than medical institutions increase, and subsequent rises and falls are repeated. Fourth, research methods based on case analysis dominate the entire stage. Fifth, the contents of the study are mainly about architectural spatial elements of healing environment design. Sixth, the scope of the study is centered on hospitals and is focused on interior space planning. Seventh, the subjects of the study show different changes in the early, mid and late periods. Research on healing environment design is based on scientific evidence, and further research on psychological and social factors should be conducted.

《诗经》英译情况研究

韩菁(Han,Jing) 조선대학교 국제문화연구원 2017 국제문화연구 Vol.10 No.1

As the first collection of Chinese poems, The Book of Songs collected poems written in about 500 years from the turn of the Shang dynasty and the Zhou dynasty to the middle of the Spring and Autumn. As the top of the Five Classics, it is not only a treasure of the traditional Chinese culture, but also the culture heritage of the world. There are 311 poems in it, which can be divided into three part Feng(Ballads), Ya(Festival Odes) and Song(Sacrificial Songs). It also adopts Fu, Bi, Xing as the technique of expression, sometimes the songs come straight to the point, and sometimes they use symbolism, all of which endow the songs sincere feelings and make them honest and unsophisticated. Being regarded as a quintessential part of our Chinese people’s culture, it has been the focus of attention from the very beginning of the Sino-Western cultural communication, and later, western missionaries and sinologists translated it into various languages. The English translation of it started in the 18 century, and it was translated into various version during over 200 years. As an important part of international cultural exchange, the Book of Songs has forcefully pushed Chinese literature known to the world.

정식 시기와 적심 횟수가 화단국화의 생장과 개화에 미치는 영향

姚婧超, 정해준, 김동찬, 이진희, 권민훈, 서병기 배재대학교 자연과학연구소 2013 自然科學論文集 Vol.24 No.1

This study was conducted to investigate optimum planting date and pinching times on the growth and flowering of garden chrysanthemum(Dendranthema grandiflorum Ramat.). 3 lines of garden mums were planted on May 16, June 13 and July 11 respectively. As planting date was delayed, all the 3 lines' plant height and plant width was reduced. 09-19-49 line got the biggest plant height and width, followed by 09-11-37 line. And 08-166-02 line was the smallest. In different planting times, the experiment group of May 16 and June 13 got the biggest plant height, plant width and stem diameter. However, in the experiment group of May 16, most of the plants got flattened. The experiment group of June 13 got the best ornamental value, and the ornamental value was reduced in the experiment group of July 11 by the short vegetative growth. 09-19-49 line got the most flowers, while in different planting time, the experiment group of May 16 got the most flowers. 09-11-37 line got the biggest flower diameter. 09-11-37 line and 09-09-51 line were planted in the containers on the rooftop. Each groups were pinched by 1, 2 and 3 times on July 2, July 26 and Aug 16 respectively. There was no effect on plant height, plant width, and stem diameter of 09-09-51 line and 09-11-37 line between 1-time-pinching group and 2-time-pinching group. But 3-time-pinching group was reduced. There was no significance in flower diameter of these two lines but the number of flowers was reduced by more pinching times. Therefore, in this experiment, the groups which were planted on June 13 got the best ornamental value and the recommended planting time is in the middle of June. Furthermore, 2 times of pinching was recommended.

Removal of Hg(II) from aquatic environments using activated carbon impregnated with humic acid

Jin, G.,Eom, Y.,Lee, T.G. Korean Society of Industrial and Engineering Chemi 2016 Journal of industrial and engineering chemistry Vol.42 No.-

The removal of Hg from aquatic environments using activated carbon (AC) impregnated with humic acid was investigated. The Hg adsorption capacity of the AC was controlled by the specific surface area and by surface functional groups, and the Hg adsorption process was exothermic and more effective at low pH. The Hg adsorption process in this study corresponds to a Lagergren pseudo-2nd-order model, indicating that it is controlled by the chemical rather than the physical properties of the AC. In addition, the equilibrium isotherm data fit the Langmuir isotherm better than the Freundlich model, indicating that the AC surfaces were uniform and that a Hg adsorbate monolayer formed at equilibrium.

南海岸地方 初期 櫛文土器의 一檢討 : 朝島具塚 出土品을 中心으로

鄭澄元 嶺南考古學會 1990 嶺南考古學 Vol.- No.7

室內수영장의 水質檢査에 의한 실험적 연구 : KMnO₄ 소비량과 Cl^(-)값 間의 相關關係 Correlation between KMnO₄demand and Cl^(-) values

이징연 대구보건대학 1992 대구보건대학 論文集 Vol.13 No.-

This study was performed to get some basic data in order to maintain the healthful water quality in the indoor swimming pool. The results are summarized as follows : 1. Correlation of KMnO₄ Demand and Cl^(-) values was r=0.9844 in Adult pool, r=0.9858 in children pool. 2. The relation equation of KMnO₄ Demand with time in Adult pool was log y=0.047 log x+0.8702, the relation equation of Cl^(-) with time in Adult pool was log y=0.041 log x+1.631. 3. The relation equation of KMnO₄ Demand with time in children pool was log y=0.090 log x+0.9475, the relation equation of Cl^(-) with time in children pool was log y=0.055 log x+1.91. 4. The supply water quantity was desirable alout 36㎥ to maintain with KMnO₄ Demand 12 p.p.m in a pool for children of 300 persons per day.

Harbart의 敎授本質論

崔定雄 대구효성가톨릭대학교 1985 연구논문집 Vol.30 No.1

The purpose of this study is to find governing principles of Herbart's methodology of instruction, that is, firstly Introduction, Secondly essence of Instruction, thirdly Steps of Instruction, fourthly development with its contribution and weakness in Herbartian method, finally conclusion. Johann Herbart(1776-1841) was a German Philosopher and a great educator known for his contributions first to formulate a science of education which based directly on ethics and psychology. He is therefore called the father of modern educational psychology and also the father of the modern science of education. From ethics he derived the end of instruction, from psychology, its method. Herbart was strongley under the influence of Lock, Lebniz, Kant, Fichte and Pestalozzi. He is usually represented as a disciple of pestalozzi and he is a Kantian. In the conflict of rationalism and empiricism of 18 century Herbart chose a middle position. He agreed with the rationalists that the soul is an entity, but he refused to accept their doctrine that Knowledge is innate. He agreed with the empirists that Knowledge arises from experience but he turned against the sensationalism of Locke and the materialixm of the french philosophers, especially illuminists. Herbart's pedagogy is that the only foundation of the whole education is instruction and educations as a science depends on a psychology. He is attempted to apply mathematics and physiological psy. to psychology and the aesthetic revelation of the world the supreme business of education, in a word, aesthetics to ethics. Knowledge comes from experience and presentations, further it is acquired by intercourse with others, especially from teachers in the work of instruction. Presentations result from the soul bringing about an inner reaction or creative process of recognition. Presentations are objects of thought which have bee presented, that is brought into consciousness. Knowledge is no longer goal, it is the only instrument to educational aims, and that knowledge is the basis of al the higher intellectual and moral development. The whole purpose of education may be summed up in the concept-marality or virtue. Virtue mean for Hebart five basic idears: ① the idear of inner freedom, ② the idea of perfection, ③ the idea of benevolence or good will, ④ the idea of right or Justice, ⑤ the idea of retrubution. Herbart's contribution to pedagogical theory is is doctrines of interest. For Herbart interest became the basic principle upon which depended all his other principles. Interest is an inner tendency making for the retention of an object of thought in consciousness. This interest comes from two laws of the mind: the law of frequency and the other the law of association. He found that the interests of man were many-sided, that they come from two main sources: man's contact with things and his relation with people. In the respective fields of interests consists of six: ① Knowledge or empirical ② sympathy ③ human ④ social ⑤ religious ⑥ aesthetic relations. Herbart's method have two principles, three process and four formal steps. The two principles are: the principle of concentration and correlation, analysis and synthetic. The three process of education are: ① Control (Regierung) which is controlled teachers of pupils behaviour, ② instruction (Unterricht) ③ discipline or Self-discipline (Zucht) He insisted that teaching procedure should be adapted to the stages discernible in the development of knowledge. He distinguished two pahses an intensive and an extensive. Vertiefung-aborption in or concentration on a subject, and Besinnung-coordination and systematisation of the results of Vertiefung. He recognize the four formal steps which are indispensable in every process of instruction, Usually termed the four steps of recitation these are: ① clearness ② association ③ system ④ method. The Herbartians have five steps: ① preparation ② presentation ③ association ④ generalization ⑤ application. Herbart's teaching method had great effect of modern education.