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유기질비료 시비량에 따른 오미자 묘목의 초기 생육특성에 관한 연구
이경아,박태호,박성용,김도현,오문국,김민수,안태진,이상원,김영국,안영섭,박충범,차선우,송범헌 忠北大學校 農業科學硏究所 2015 農業科學硏究 Vol.31 No.1
This study was carried out to have basic and applied informations to find the optimum nitrogen fertilization level for organic cultivation and to investigate early growth characteristics of Schizandra chinensis Baillon. Different application levels of the organic fertilizer were based on the recommended rate of N fertilizer. Five different levels of the organic fertilizer were applied to S. chinensis and early growth responses such as the length of vine, the diameter of vine, the number of leaves, and the number of branches were investigated. Lengths of main and total vines were shown longer at the level of 1.0 time organic fertilizer than those at others. The vine length at early growth was the highest at the level of 1.0 time organic fertilizer among the treatments. Stem diameter was shown thicker level of 1.0 time organic fertilizer than others on August and September, whereas on October it was the thickest in the conventional cultivation. Number of leaf was increased higher at the level of 1.0 and 2.0 times organic fertilizer than that in the compared to conventional. The number of branches was appeared the lowest at the level of 1.0 time organic fertilizer at early growth, however they were increased through progressing their growth stages. They were not significantly difference among the treatments except for the level of 2.0 times organic fertilizer. Based on the research results, the growth of S. chinensis was commonly higher at the level of 1.0 time organic fertilizer compared to other treatments.
Wen, Min,Zheng, Jin Hai,Choi, Jin Myung,Pei, Jian,Li, Chun-Hao,Li, Song-Yuan,Kim, In-Young,Lim, Sa-Hoe,Jung, Tae-Young,Moon, Kyung-Sub,Min, Jung-Joon,Jung, Shin Elsevier 2018 Cancer letters Vol.433 No.-
<P><B>Abstract</B></P> <P>Glioma is one of the most devastating and refractory cancers. The main factors underlying therapeutic failure include extremely invasive characteristics and lack of effective methods for drug delivery. Attenuated <I>Salmonella</I> strains presented a high concentration of tumor targets in various types of cancer models, suggesting a role as potential vectors for drug delivery. In this study, we genetically engineered an attenuated strain of <I>Salmonella</I> as an anti-invasive vector for the targeted delivery and expression of tissue inhibitor of metalloproteinases 2 (TIMP-2) in an orthotopic nude mouse model of glioma. The bioluminescence signals related to tumor size significantly declined in the TIMP-2-expressing <I>Salmonella</I> (SLpTIMP-2)-treated group compared with the control group. Compared with the control group with a survival rate of an average of 33 days, the SLpTIMP-2 group showed an extended survival rate by nearly 60% and lasted an average period of 53 days with TIMP-2 induction. These results indicated the promising therapeutic potential of <I>S. typhimurium</I> for targeted delivery and secretion of TIMP-2 in glioma.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Intracranial injection of <I>Salmonella</I> has been demonstrated to be a more effective than tail vein injection. </LI> <LI> Treatment with TIMP-2-expressing bacteria showed down regulation of MMP-2 in orthotopic glioma. </LI> <LI> TIMP-2-expressing bacteria significantly inhibited tumor growth and elongated animal survive. </LI> </UL> </P>
아몬드와 대두의 혼합비율과 가공공정을 달리하여 제조한 아몬드 청국장의 품질 특성
Wen-Di Jin,어지현,황수정,범희주,은종방 全南大學校 農業科學技術硏究所 2012 農業科學技術硏究 Vol.47 No.-
Almond cheonggukjang was made and evaluated in order to improve nutritional value and quality of cheonggukjang with different addition levels of almond and soybean, 4:6, 5:5 and 6:4 (w/w). To make almond cheonggukjang, Bacillus subtilis was added to mixture of almond and soybean then fermented at 42℃ for 48 hours. Physicochemical, microbiological properties and sensory evaluation for almond cheonggukjang were conducted. In color, L* and b* values of almond cheonggukjang increased with increasing addition levels of almond. As addition levels of almond increased, there was no change in pH value of almond cheonggukjang. Slime production of almond cheonggukjang with 5:5 (w/w) ratio of almond and soybean was the highest among all samples. These results showed that the almond cheonggukjang exhibited effective growth of microorganisms with increasing addition levels of soybean. The amino nitrogen contents of almond cheonggukjang ranged from 164.85±1.12 mg% to 173.25±1.16 mg%. Almond cheonggukjang with 6:4 (w/w) ratio of almond and soybean and prepared by steaming showed the best results in sensory evaluation, compared to those prepared by steaming with 4:6 (w/w) and 5:5 (w/w) ratios. In conclusion, almond cheonggukjang added with 6:4 (w/w) ratio of almond and soybean and prepared by steaming was the best formula to improve nutritional value and quality of cheonggukjang.
Chemical Constituents from the Leaf and Twig of Acer okamotoanum Nakai and their Cytotoxicity
Jin, Wen-Yi,Min, Byung-Sun,Youn, Ui-Jung,Hung, Tran-Manh,Song, Kyung-Sik,Seong, Yeon-Hee,Bae, Ki-Hwan The Korean Society of Medicinal Crop Science 2006 한국약용작물학회지 Vol.14 No.2
As a result of cytotoxic compounds against cancer cell lines from natural sources, senven compounds were isolated from the leaf and twig of Acer okamotoanum Nakai. The compounds (1-7) were identified as ethyl gallate (1), methyl gallate (2), gallic acid (3), trans $resveratrol-3-O-{\beta}-D-glucopyranoside$ (4), acertannin (5), nikoenoside (6), and fraxin (7) by physicochemical and spectroscopic data (including mp, UV, IR, MS, $^1H-NMR,\;^{13}C-NMR$, DEPT, and HMBC) in comparison with those of published papers. All the compounds were tested for their cytotoxic activity against L1210, HL-60, K562, and B16F10 cancer cell lines in vitro by MTT assay method. Compounds 1-3 and 5 showed cytotoxic activity against all tested cell lines with $IC_{50}$ values ranged from 12.5 to $72.2\;{\mu}M$. Of the compounds, methyl gallate (2) exhibited the most potent cytotoxic activity against L1210, HL-60, K562, and B16F10 tumor cells with $IC_{50}$ values of 12.5, 48.3, 22.8, and $22.8\;{\mu}M$, respectively. Other compounds did not show any cytotoxic activity against four cancer cell lines.
Regularized solution to the Fredholm integral equation of the first kind with noisy data
Jin Wen,Ting Wei 한국전산응용수학회 2011 Journal of applied mathematics & informatics Vol.29 No.1
In this paper, we use a modified Tikhonov regularization method to solve the Fredholm integral equation of the first kind. Under the assumption that measured data are contaminated with deterministic errors, we give two error estimates. The convergence rates can be obtained under the suitable choices of regularization parameters and the number of measured points. Some numerical experiments show that the proposed method is effective and stable.
Antioxidant Activity of Cleomiscosins A and C Isolated from Acer okamotoanum
Wen Yi Jin,Phuong Thien Thuong,Nguyen Duy Su,민병선,손건호,장현욱,김현표,강삼식,석대은,배기환 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.3
Phytochemical investigation of Acer okamotoanum leaf and twig led to the isolation of two coumarinolignans, cleomiscosin A (1) and cleomiscosin C (2). Here, we found that 2 dose-dependently inhibits LDL oxidation mediated by either catalytic copper ions (Cu2+) or free radicals generated with the azo compound 2,2'-azobis-(2-amidinopropane)dihydro-chloride (AAPH) with IC50s of 29.5 and 11.9 µM, respectively. By electrophoretic analysis, we also observed that 2 protects apolipoprotein B-100 (apoB-100) against Cu2+-induced fragmentation (65.3% inhibition at 5 µM). Furthermore, fluorescence analyses clearly indicated that both 1 and 2 protect against the oxidative modification of apoB-100 induced by either Cu2+ or HOCl (1, IC50s of 13.4 and 8.1 µM, respectively; 2, IC50s of 23.6 and 3.9 µM, respectively). These findings suggest that 1 and 2 could be beneficial in preventing LDL oxidation in atherosclerotic lesions.
Jin Wen,Jun-Feng Cheng 한국전산응용수학회 2017 Journal of applied mathematics & informatics Vol.35 No.5
This paper investigates the inverse problem of determining an unknown heat radiative coefficient, which is only time-dependent. This is an ill-posed problem, that is, small errors in data may cause huge deviations in determining solution. In this paper, the existence and uniqueness of the problem is established by the second Volterra integral equation theory, and the method of trace-type functional formulation combined with finite difference scheme is studied. One typical numerical example using the proposed method is illustrated and discussed.