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Jin-ao Duan,Liuying Wang,Shihui Qian,Shulan Su,Yuping Tang 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.8
A new prenylated dihydrobenzofuran derivative (1), was isolated from the rhizomes of Atractylodes lancea DC (Asteraceae), along with ten known compounds, including atractylenolide II (2), ϕ-taraxasteryl acetate (3), taraxerol acetate (4), β-sitosterol (5), stigmasterol (6), β-eudesmol (7), atractylenolide III (8), atractylenolide IV (9), daucosterol (10), and stigmasterol 3-O-β-D-glucopyranoside (11). The structure of the new compound (1) was elucidated as trans-2-hydroxyisoxypropyl-3-hydroxy-7-isopentene-2,3-dihydrobenzofuran-5-carboxylic acid by the combination of 1D, 2D NMR analysis and mass spectrometry, and it was the first reported 2,3-dihydrobenzofuran derivative having a carboxyl residue at C-5 and an isopentene moiety at C-7 contemporaneously. In addition, compound 1 exhibited significant cytotoxicity against cancer cell lines HCT-116 and MKN-45.
Duan, Jin-Ao,Wang, Liuying,Qian, Shihui,Su, Shulan,Tang, Yuping 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.8
A new prenylated dihydrobenzofuran derivative (1), was isolated from the rhizomes of Atractylodes lancea DC (Asteraceae), along with ten known compounds, including atractylenolide II (2), $\varphi$-taraxasteryl acetate (3), taraxerol acetate (4), $\beta$-sitosterol (5), stigmasterol (6), $\beta$-eudesmol (7), atractylenolide III (8), atractylenolide IV (9), daucosterol (10), and stigmasterol 3-O-$\beta$-D-glucopyranoside (11). The structure of the new compound (1) was elucidated as trans-2-hydroxyisoxypropyl-3-hydroxy-7-isopentene-2,3-dihydrobenzofuran-5-carboxylic acid by the combination of 1D, 2D NMR analysis and mass spectrometry, and it was the first reported 2,3-dihydrobenzofuran derivative having a carboxyl residue at C-5 and an isopentene moiety at C-7 contemporaneously. In addition, compound 1 exhibited significant cytotoxicity against cancer cell lines HCT-116 and MKN-45.
Comparative Study of Autophagy in Oxaliplatin-Sensitive and Resistant SNU-C5 Colon Cancer Cells
Boo Sun-Jin,Piao Mei Jing,Kang Kyoung Ah,Zhen Ao Xuan,Fernando Pincha Devage Sameera Madushan,Herath Herath Mudiyanselage Udari Lakmini,Lee Seung Joo,Song Seung Eun,Hyun Jin Won 한국응용약물학회 2022 Biomolecules & Therapeutics(구 응용약물학회지) Vol.30 No.5
Few studies have evaluated the role of autophagy in the development of oxaliplatin (OXT) resistance in colon cancer cells. In this study, we compared the role of autophagy between SNU-C5 colon cancer cells and OXT-resistant SNU-C5 (SNU-C5/OXTR) cells. At the same concentration of OXT, the cytotoxicity of OXT or apoptosis was significantly reduced in SNU-C5/OXTR cells compared with that in SNU-C5 cells. Compared with SNU-C5 cells, SNU-C5/OXTR cells exhibited low levels of autophagy. The expression level of important autophagy proteins, such as autophagy-related protein 5 (Atg5), beclin-1, Atg7, microtubule-associated proteins 1A/1B light chain 3B I (LC3-I), and LC3-II, was significantly lower in SNU-C5/OXTR cells than that in SNU-C5 cells. The expression level of the autophagy-essential protein p62 was also lower in SNU-C5/OXTR cells than in SNU-C5 cells. In SNUC5/ OXTR cells, the production of intracellular reactive oxygen species (ROS) was significantly higher than that in SNU-C5 cells, and treatment with the ROS scavenger N-acetylcysteine restored the reduced autophagy levels. Furthermore, the expression of antioxidant-related nuclear factor erythroid 2-related factor 2 transcription factor, heme oxygenase-1, and Cu/Zn superoxide dismutase were also significantly increased in SNU-C5/OXTR cells. These findings suggest that autophagy is significantly reduced in SNU-C5/OXTR cells compared with SNU-C5 cells, which may be related to the production of ROS in OXT-resistant cells.
Jin Won Hyun,Kyoung Ah Kang,장예,MEIJING PIAO,Ao Xuan Zhen,Herath Mudiyanselage Udari Lakmini Herath,Pincha Devage Sameera Madushan Fernando 건강기능식품미래포럼 2022 건강기능식품미래포럼 학술지 Vol.2 No.4
Luteolin has been reported to possess apoptotic and antitumor properties. Apoptosis induced by endoplasmic reticulum (ER) stress becomes a potential target for chemotherapeutic strategies since drug resistance develops less if anticancer action is attributed to this apoptosis. In the present study, we raised a possibility that luteolin may induce apoptosis via inducing the ER stress and attempted to test this possibility using HT-29 human colon cancer cells. When HT-29 cells were treated with luteolin, decrease of cell viability, DNA fragmentation and the increase of sub-G1 population were observed, confirming that this compound induced apoptosis. Simultaneously, the cells showed typical signs observed in the response of ER stress, which were Ca2+ overloading in cytosol and mitochondria, phosphorylation of PKR-like ER kinase (PERK) and its downstream proteins: eukaryotic initiation factor-2α and inositol requiring protein1(IRE1), splicing of ER stress-specific X-box transcription factor-1 (XBP-1), cleavage of activating transcription factor 6 (ATF6) as well as up-regulation of glucose-regulated protein-78 (GRP78) and CCAAT/enhancer-binding protein-homologous protein (CHOP). However, when HT-29 cells transfected with siCHOP RNA were treated with luteolin, its effects on decrease of cell viability, DNA fragmentation and sub-G1 population were significantly reduced, suggesting that the luteolin-induced apoptosis is mediated by inducing ER stress. These results support that luteolin has the potential as an agent for cancer prevention or treatment.
Niacinamide Protects Skin Cells from Oxidative Stress Induced by Particulate Matter
( Ao Xuan Zhen ),( Mei Jing Piao ),( Kyoung Ah Kang ),( Pincha Devage Sameera Madushan Fernando ),( Hee Kyoung Kang ),( Young Sang Koh ),( Joo Mi Yi ),( Jin Won Hyun ) 한국응용약물학회 2019 Biomolecules & Therapeutics(구 응용약물학회지) Vol.27 No.6
Niacinamide (NIA) is a water-soluble vitamin that is widely used in the treatment of skin diseases. Moreover, NIA displays antioxidant effects and helps repair damaged DNA. Recent studies showed that particulate matter 2.5 (PM<sub>2.5</sub>) induced reactive oxygen species (ROS), causing disruption of DNA, lipids, and protein, mitochondrial depolarization, and apoptosis of skin keratinocytes. Here, we investigated the protective effects of NIA on PM<sub>2.5</sub>-induced oxidative stress in human HaCaT keratinocytes. We found that NIA could inhibit the ROS generation induced by PM<sub>2.5</sub>, as well block the PM<sub>2.5</sub>-induced oxidation of molecules, such as lipids, proteins, and DNA. Furthermore, NIA alleviated PM<sub>2.5</sub>-induced accumulation of cellular Ca<sup>2+</sup>, which caused cell membrane depolarization and apoptosis, and reduced the number of apoptotic cells. Collectively, the findings show that NIA can protect keratinocytes from PM<sub>2.5</sub>-induced oxidative stress and cell damage.
Adaptive Control with a Fuzzy Tuner for Cable-based Rehabilitation Robot
Jin Yang,Hang Su,Zhijun Li,Di Ao,Rong Song 제어·로봇·시스템학회 2016 International Journal of Control, Automation, and Vol.14 No.3
Since there are external uncertainties in the environment and the dynamic properties of human arm aretime-varying during movement, it is difficult to achieve good tracking performance during robot-aided rehabilitation. The purpose of this study is to develop an adaptive controller with a fuzzy tuner for a cable-based rehabilitationrobot. The fuzzy tuner can adjust the control parameters according to position error and the change of error, thus thetime-varying control parameters can be optimized by this tuner. To verify the proposed controller, simulation andexperiment studies using the cable-based rehabilitation robot are carried out. The rehabilitation robot is employedto complete two facilitation movements in the experiment. Results show that the adaptive controller can attain bettercontrol performance after tuning the control parameters.
Ao Xuan Zhen,Mei Jing Piao,강경아,FERNANDO PINCHA DEVAGE SAMEERA,강희경,고영상,JIN-WON HYUN 대한암예방학회 2019 Journal of cancer prevention Vol.24 No.2
Background: Reactive oxygen species (ROS) are involved in various cellular diseases. Excessive ROS can cause intracellular oxidative stress, resulting in a calcium imbalance and even aging. In this study, we evaluated the protective effect of esculetin on oxidative stress-induced aging in human HaCaT keratinocytes. Methods: Human keratinocytes were pretreated with esculetin for 30 minutes and treated with H2O2. Then, the protective effects on oxidative stress-induced matrix metalloproteinase (MMP)-1 were detected by Flou-4-AM staining, reverse transcription-PCR, Western blotting, and quantitative fluorescence assay. Results: Esculetin prevented H2O2-induced aging by inhibiting MMP-1 mRNA, protein, and activity levels. In addition, esculetin decreased abnormal levels of phospho-MEK1, phospho-ERK1/2, phospho-SEK1, phospho-JNK1/2, c-Fos, and phospho-c-Jun and inhibited activator protein 1 binding activity. Conclusions: Esculetin prevented excessive levels of intracellular calcium and reduced the expression levels of aging-related proteins. (J Cancer Prev 2019;24:123-128)
( Ao Xuan Zhen ),( Mei Jing Piao ),( Yu Jae Hyun ),( Kyoung Ah Kang ),( Yea Seong Ryu ),( Suk Ju Cho ),( Hee Kyoung Kang ),( Young Sang Koh ),( Mee Jung Ahn ),( Tae Hoon Kim ),( Jin Won Hyun ) 한국응용약물학회 2019 Biomolecules & Therapeutics(구 응용약물학회지) Vol.27 No.4
Purpurogallin, a natural phenol obtained from oak nutgalls, has been shown to possess antioxidant, anticancer, and anti-inflammatory effects. Recently, in addition to ultraviolet B (UVB) radiation that induces cell apoptosis via oxidative stress, particulate matter 2.5 (PM<sub>2.5</sub>) was shown to trigger excessive production of reactive oxygen species. In this study, we observed that UVB radiation and PM<sub>2.5</sub> severely damaged human HaCaT keratinocytes, disrupting cellular DNA, lipids, and proteins and causing mitochondrial depolarization. Purpurogallin protected HaCaT cells from apoptosis induced by UVB radiation and/or PM<sub>2.5</sub>. Furthermore, purpurogallin effectively modulates the pro-apoptotic and anti-apoptotic proteins under UVB irradiation via caspase signaling pathways. Additionally, purpurogallin reduced apoptosis via MAPK signaling pathways, as demonstrated using MAPK-p38, ERK, and JNK inhibitors. These results indicate that purpurogallin possesses antioxidant effects and protects cells from damage and apoptosis induced by UVB radiation and PM<sub>2.5</sub>.
Ao Wang,Housheng Zhang,Junjie Jiang,Duo Jin,Shengjie Zhu 전력전자학회 2023 JOURNAL OF POWER ELECTRONICS Vol.23 No.2
In consideration of the shortcomings of the large torque and flux ripples in direct torque control (DTC), and the insufficient utilization of the duty cycle module in traditional DTC based on the duty cycle modulation of PMSMs, a DTC method based on deadbeat control of the torque and flux is proposed in this paper. In the synchronous rotation, coordinate system positioned with the stator flux vector, the reference voltage vector is calculated in accordance to the deadbeat control method. Six virtual voltages are constructed in such a manner that two adjacent basic voltage vectors are evenly divided in one control cycle. The effective voltage vectors are expanded to 12. The effective voltage vector closest to the phase angle of the reference voltage vector is determined to be the optimal voltage vector. Then, the duty cycle is introduced to further reduce the error between the final output voltage vector and the voltage vector required by the system. Simulation comparisons are carried out for DTC, traditional DTC based on duty cycle modulation, and the proposed DTC. The obtained simulation results demonstrate that the proposed DTC can effectively suppress both torque ripple and flux ripple, improve the utilization rate of the duty cycle module, and reduce the stator current distortion rate.