흰쥐에서 고환절제와 분만전 Testosterone 투여가 안쪽시각로앞구역의 Galanin 면역양성세포 발현 미치는 영향

박인식,김진택 대한해부학회 1997 Anatomy & Cell Biology Vol.30 No.3

흰쥐중심편도체핵에서 Neurotensin 면역양성세포의 다리옆핵으로의 투사

박인식,최정화,박봉수,백선용,김봉선,김재봉,김진정 대한해부학회 1996 Anatomy & Cell Biology Vol.29 No.2

뇌실결핵 (paraventricular nuclei)에서 탈수자극에 의한 neuropeptide 면역반응양상 변화

박인식,김진택 대한해부학회 1999 Anatomy & Cell Biology Vol.32 No.1

Lipopolysaccharide로 유발된 생쥐 무릎 관절염 완화에 미치는 진통산의 효과 : 윤활관절막과 섬유관절막의 형태 변화를 중심으로 Based on the Morphological Change of Synovial Membrane and Fibrous Membrane

김진택,최홍식,안상현,박인식,강윤호,김호현,이해풍 동국대학교 경주대학 1999 東國論集 Vol.18 No.-

Knee joint BALB/C mice were administered with JINTONGSAN(JTS) extract after Lipopolysaccaride(LPS) injection were observed to investigate the anti-inflammatiory effect of JTS for arthritis. The JTS extract(3.3 ㎖/㎏/day) were daily administered to mice suffered from arthritis of knee joint induced by LPS injection, as dose of 300㎖/㎏. The specimen were fixed in 4% paraformaldehyde and were decalcificate in EDTA solution for 4 Weeks. In synovial membrane, the hyperplasia of synovial lining cell(fibroblast like synoviocytes) on JTS group were diminished then LPS group. The number of synovial lining cell(synovial phagocytic cells) with filopodia and the infiltrated of lymphocyte on JTS group were decreased. In fibrous membrane, The region of fibrosis on JTS group were mitigated than LPS group and the appearance of fibroblast and migrated cell, as neutrophil leukocytes and lymphocyte, were decreased. As results indicated that JTS was effective in anti-inflammatory reaction for LPS-induced arthritis.

매립지 조기안정화에 따른 침출수 및 매립가스 분포 특성 연구

박진식,안철우,박출재,김수생 동아대학교 환경문제연구소 1999 硏究報告 Vol.22 No.1

This study was conducted to establish primary data for the stabilization assessment of expired landfill site with analysis of leachate and landfill gas Based on the results of the experiments, BOD and COD concentration of leachate became low after earlier stabilization facilities and COD/COD ratio of leachate was investigated average 0.18 in 1998. Variation of CH₄concentration of landfill gas in spring and summer were higher than autumn and winter and CH₄concentration was investigated average 14.2% in Dec. 1998.

긴볼레기말의 항고지혈증 효과에 관한 연구 : Triton WR-1339 주사에 의한 고지혈증 유발 생쥐의 간세포내 지방 축적 감소를 중심으로 Based on the Decreasing Effect of Lipid Accumulation in Hepatocyte of Murine with Hyperlipidemia induced by Triton WR-1339

박인식,안상현,정재만,강윤호,이해풍,서귀문,홍용기,김호현,김진택 동국대학교 한의학연구소 1999 東國韓醫學硏究所論文集 Vol.7 No.2

본 연구는 긴볼레기말 추출물의 항고지혈증 효과를 조사하기 위해 ICR 생쥐에 TritonWR-1339(TX) 복강주사로 인위적인 고지혈증을 유발시킨 후 긴볼레기말 추출물(30㎎/㎏)를 복강주사하여 시간의 경과에 따른 간세포내에서의 지방 축적 변화를 조직화학적으로 관찰하였다. TX 주사후 그물구조의 세포질출 가진 간세포가 간엽 전체에서 관찰되었고, 일부 간소엽에서는 간세포 손상으로 인한 간세포판 소실이 나타났다. 또한 간세포내 지방축척도 증가하여 전체 간소엽의 간세포에서 지방의 과출현을 확인 할 수 있었고, 지방의 크기도 대조군에 비해 증가된 것으로 관찰되었다. 그러나 긴볼레기말 추출물 주사군에서는 그물구조의 세포질을 가진 간세포의 수가 TX 주사군에 비해 감소되었고, 대부분의 간소엽에서 정상적인 간세포판의 배열을 확인할 수 있었다. 간세포내의 지방 축적과 크기도 감소된 경향으로 관찰되었다. 이상의 결과로 볼 때 해조류 긴볼레기말 추출물은 고지혈증이 유발된 생쥐 간세포 내에서의 과도한 지방축적을 감소시키는 항고지혈증 효과를 하는 것으로 사료된다. Hepatic tissues of ICR mouse were intraperitoneally injeced with Colpomenia bullosa(CB) Extract after Triton WR-1339(TX) injection were observed to investigate the antihyperlipermic effect of CB extract for hyperlipidemic hepatic tissue caused by destruction of lipid metabolism. The hepatic tissues were obtained at hour-24, 48, and 72 after TX injection with CB extract treatment. And then these specimen were fixed in 10% neutral buffer solution and were cryocut. The tissue stained by H&E for general morphology and sudan black B for lipid distribution. The increase of hepatocyte having rneshlike cytoplasm were shown in all hepatic lobules after TX injection and the hepatic plates were disappeared in the region of meshlike hepatocyte aggregation, But the hepatocyte having meshlike cytoplasm were disappeared and hapatic plate were rearranged in CB extract injected mouse. The number of blue black colored lipid drop in hepatic cytoplasm of mouse injected with TX were increased and the size of lipid drop were enlarged. But the number of lipid drop in hepatic cytoplasm of mouse treated CB extract were decreased and the size of lipid drop were diminished. As results indicated that the accumulation of lipid drop caused by TX injection were mitigated by the antihyperlipidermic effect of CB extract.

활성슬러지법을 이용한 화장품폐수 처리에 관한 연구

박진식,문추연,김동일 경운대학교 산업기술연구소 2001 産業技術硏究論文誌 Vol.3 No.2(B)

In this study, Activated Sludge was operated for treatment of cosmetic wastewater and combined with sewage The results from this study are follows. During the experiments, average COD and BOD concentrations of raw wastewaters were ranged 690∼1,010 ㎎/l, 550 ∼ 880㎎/l respectively, and fluctuated sharply with time. COD removal efficiency was 73.6% at HRT 12hr. It is higher removal efficiency than HRT 4hr 62.6%, HRT 8hr 69.4% when cosmetic wastewater combine with sewage, COD and BOD removal efficiencies were higher than wastewater only, therefore treatment of combined sewage was effective.

4-Stage BNR공정을 이용한 하수처리

박진식,문추연 경운대학교 산업기술연구소 2001 産業技術硏究論文誌 Vol.3 No.2(C)

Recently in Korea and developed countries, nitrogen and phophorus removal are the main target to improve effluent qualities, in order to protect discharge basins from eutrofication by N and P. Introduction of an advanced treatment function to existing wastewater treatment systems, which can be removed nitrogen and phophorus as well as BOD, COD and SS, have been required to protect water resources from domestic and industrial wastewaters. This study was undertaken to investigate the organic matter and nutrient removal efficiencies and the operating parameters for treatment of municipal wastewater by using laboratory scale BNR(Biological nutrient removal) process. The average removal efficiencies of BOD and COD were 91.7% and 81.9% respectively. The nutrient removal efficiencies were NH4-N 96.2%, T-N 67.8%, PO4-P 72.2% and T-P 70.7%, respectively.

Triton WR-1339 주사에 의한 고지혈증 유발 생쥐 신장에 미치는 참홑파래 추출물의 지방축적 억제 영향

박인식,안상현,최난희,김진택 동국대학교 한의학연구소 1998 東國韓醫學硏究所論文集 Vol.6 No.2

본 실험은 참흩파래 추출물의 항고지혈증 효과의 조사를 위해 시행된 것으로 ICR 생쥐에 Triton WR-1339(TX) 복강주사로 고지혈증을 유발시킨 후 참흩파래 추출물(30mg/kg)를 복강주사하여 시간의 경과에 따른 신장조직의 일반적인 형태변화와 콜레스테롤을 비롯한 지방입자 축적양상 변화를 관찰하였다. TX 주사 후 신장조직에서 나타나는 일반적인 형태변화는 콩팥소체의 혈관극과 요세관극 모두에서 단층편평상피인 보우만주머니의 외벽이 비후되어 단층입방상피의 형태로 바뀌었으며 요세관 공간도 확장되었다. 지방입자는 사구체 전지역, 근위곱슬세관, 원위곱슬세관 그리고 헨레고리에서 침착이 증가된 것으로 나타났으며, 지방입자의 크기도 증가하였다. 또한 콜레스테롤 입자도 전 지역에서 월등하게 증가한 분포양상을 보였다. 그러나 TX 주사 후 참흩파래 추출물 처리군에서는 보우만주머니 외벽세포의 비후와 요세관공간의 확장을 일부 콩팥소체에서만 관찰할 수 있었으며, 대부분은 정상적인 신장형태를 유지하고 있는 것으로 나타났다. 한편 지방입자는 TX 주사군에 비해 그 분포와 크기가 감소한 것 나타났으며, 또한 콜레스테롤입자도 일부 지역에서 나타나 그 분포가 감소된 것으로 나타났다. 이상의 결과로 볼 때 참흩파래 추출물은 TX 주사로 고지혈증이 유발된 생쥐에 대해 콜레스테롤을 비롯한 지방축적을 억제하는 항고지혈증 효과를 하는 것으로 사료된다. Renal tissues of ICR mouse were intraperitoneally injeced with Monostroma nitidum extract(ME) after Triton WR-1339(TX) injection were observed to investigate the lipid accumulation suppressive effect of ME. The renal tissues were obtained at hour 24, 48, and 72 after TX injection that were fixed in fromol-calcium solution and were cryocut. The tissue stained by H&E for general morphology, sudan black B for lipid and perchloric acid-naphthoquinone method for cholesterol. The parietal layer of Bowman's capsule is swelled as cuboidal type at hour 48 after TX injection and the lipid blot and cholesterol particle were noticeably increased in glomerulus than control of group and these appeared in convoluted tubule, collecting tubule, and Helen's loop. But the parietal layer of Bowman's capsule was shaped as squamous type that was looked like as normal morphology in ME treated group after TX injection and the lipld blot and cholestero1 particle were considerably decreased in glomerulus than TX group. As results indicated that the accumulation of lipid including cholesterol caused by TX injection were mitigated in renal tissues by the antihyperlipidermic effect of ME.

Cyclosporin A 로 유도된 생쥐 림프절의 세포성 면역억제에 관한 면역조직화학적 연구 : T 림프구, IL-2 수용기 및 NK세포의 변화를 중심으로 Based on the change of T lymphocytes, Il-2 receptors, and NK cells

김진택,박인식,안상현,최난희,김동환 동국대학교 한의학연구소 1998 東國韓醫學硏究所論文集 Vol.6 No.2

본 실험은 cyclosporin A(CsA)에 의한 시간의 경과에 따른 림프절에서의 세포성 면역억제를 조사하기 위해서 시행된 것으로 BALB/C계 생쥐에 10일동안 CsA(45mg/kg/day) 투여 후 림프절에서의 T 림프구, IL-2 수용기 그리고 자연살해(NK)세포의 분포 변화를 관찰하였다. 대조군의 림프절에서는 L3T4(CD4)에 양성반응을 보이는 도움 T림프구, Ly2(CD8)에 양성반응을 보이는 세포독성 T 림프구 그리고 CD25R에 양성반응을 보이는 IL-2 수용기를 가진 세포는 곁피질(paracortex)과 수질동(medullary sinus)에서 분포하였다. CsA투여 후 처음 3일까지는 이들 양성반웅세포의 분포 변화는 없었으며 양성반응성의 변화도 없었다. 그러나 CsA 투여 7일부터 양성반응 세포수의 감소와 양성반응성의 약화가 관찰되기 시작하였으며 이러한 변화는 시간이 경과하여 14일에 이르렀을 때 가장 큰 감소추세로 나타났다. 한편 NKl.1(CD56)에 양성반응을 보이는 자연살해세포는 피질과 수질에 분포하였으며 CsA 투여 후 시간의 경과에 따라 양성반응 세포수가 감소하였으며, 이러한 감소는 14일에서 가장 큰 것으로 나타났다. 이상의 결과로 미루어보아 CsA 투여는 림프절에서의 IL-2 분비저해를 통해 T 림프구와 NK세포의 활성을 차단하여 선택적이면서 효과적인 세포성 면역억제작용을 하고 있는 것으로 사료된다. Cyclosporin A(CsA) is a selective immunosuppressive agent that has been credited with improved survival of solid organ allografts. Lymph node of BALB/C mouse administered CsA immunohistochemically observed to understand immunosuppressive effects of CsA on T lymphocytes, IL-2 receptors, and natural killer NK cells in lymph node. CsA orally administered daily for 10days at the dose 4smg/kg/day/. The lymph node were obtained at day 3, 7, and 14 after CsA administration and embeded with paraffin, and then stained by following ABC method that used monoclonal antibody including L3T4(CD4), Ly2(CD8), IL-2R(CD25), and NK-1.1(CD56). There were little changes of reactive degree and number of helper T lymphocytes, cytotoxic T lymphocytes, IL.-2 receptors, and NK cells at day 3 after CsA administration, but they began to decrease at day 7. These decrease were greatest at day 14. The helper T lymphocytes, cytotoxic T lymphocytes, IL-2 receptors, and NK cells distributed in paracortex and medullary sinus. These results indicated that the secretion of IL-2 began to decrease at day 7 after CsA administration and subsequently to suppress T lymphocytes and NK cell as components of cell-mediated immunity.