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Jin Ho Boo,Eunseok Kim,Byung Chan Kwon,Myung Jo Seo,Ji Man Kim,Ji Bong Joo,Dohyung Kang,No-Kuk Park 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.123 No.-
This study investigated the catalytic activity of vanadium-manganese supported on USY-zeolite as a catalystfor low-temperature NO removal, and embedded the powder catalyst in PTFE filter of bag filter. TheV2O5-MnO2/USY-zeolite catalyst was prepared using the co-impregnation method, and the V2O5/MnO2ratio was 0/10, 2.5/7.5, 5/5, 7.5/2.5, or 10/0. The catalytic activity test for NH3-SCR(selective catalyticreduction) of NO was performed at 150–230 C. An enhanced NO conversion above 60% was exhibitedin the low-temperature region below 230 C, and the NO removal efficiency increased as the MnO2 contentincreased. The NH3-TPD and NO-TPD(Temperature Programmed Desorption) analysis confirmed thatthe NH3 adsorption of the catalyst more significantly influences the NO removal performance than the NOadsorption. As the MnO2 content on the catalysts increased, the strength and amount of adsorbed NH3increased, resulting in enhanced NO conversion. The catalyst-embedded PTFE (polytetrafluoroethylene)fiber was prepared by extruding physically mixed PTFE and catalyst powder. Scanning electron microscopy/energy dispersive X-ray spectroscopy confirmed that the catalyst was well dispersed on the surfaceand inside the PTFE fiber. The NO removal performance of the catalyst included PTFE fiber increased asthe amount of the catalysts added was increased.
심재구 ( Jae-goo Shim ),아프잘아킬 ( Afzal Aqeel ),최보미 ( Bo-mi Choi ),이정현 ( Jung-hyun Lee ),곽노상 ( No-sang Kwak ),임호진 ( Ho-jin Lim ) 한국물환경학회(구 한국수질보전학회) 2016 한국물환경학회지 Vol.32 No.4
N-nitrosamines are a class of carcinogenic chemicals that can pose significant hazards to the human life. Ultraviolet (UV) light irradiation is considered as one of the effective methods to reduce N-nitrosamines in the aqueous phase. This study aimed to investigate the pH influence on UV photodegradation of N-nitrosamines (i.e., N-nitrosodibutylamine (NDBA) and N-nitrosopyrrolidine (NPYR)) closely related to water treatment. Photodegradation rate constants of NDBA and NPYR remained between 3.26×10-2 L/W-min to 5.08×10-3 L/W-min and 1.14×10-2 L/W-min to 2.80×10-3 L/W-min at pH2-10, respectively. This study also focused on the formation of oxidized products (i.e., primarily NO2- and NO3-). Under slightly acidic and eutral conditions, NO2- formation was more prevalent than NO3- formation, while under strong acidic conditions, NO3- was more prevalent. There was no significant change in total organic carbon (TOC) and total nitrogen (TN), suggesting negligible loss of N-nitrosamines and degradation products from the system. NDBA was easily photodegraded than NPYR. This study also demonstrated that a lower pH is a favorable condition for photolytic degradation of N-nitrosamines in water.
단클론 항체를 이용하여 정제한 톡소포자충 30 kDa 항원의 면역학적 특성
이영화,노태진,신대환,Lee, Yeong-Hwa,No, Tae-Jin,Sin, Dae-Hwan 대한기생충학회 1997 The Korean Journal of Parasitology Vol.35 No.1
The molecular weight 30 kDa membrane protein of Toxoplusma Sondii (Toxoplasma 30 kDa) apparently conserved in most strains of T. gondii and sera of infected hosts. The present study aimed to elucidate Toxoplasmc 30 kDa as a useful diagnotic antigen for serodiagnisis of toxoplasmosis by ELISA and for induction of protective immunity. Murine spleen cells immunized with the membrane antigen of T. gondii were fused with mouse Sp2/0-Ag 14 myeloma cells. Out of 8 clones selected, five were IgG2b, the others belonged to IgG 1 and IgG2a. The 30 kDa antigen was distributed mainly on the surface membrane of tachyzoites by indirect fluorescence method. Murine peritoneal macrophages which were activated by 30 kDa antigen produced more amounts of NO2 compared with crude antigen-treated group, however there were no significant differences in toxoplamacidal activity between the two groups. Higher specificity of Toxoplosma 30 kDa antigen was recognized for serodiagnosis of toxoplasmosis than the crude antigen. From these results, ToxopLasmo 30 kDa antigen enhances the cytotoxic effect of macrophages as well as a more reliable means for the serodiagnosis of toxoplasmosis by ELISA. Key words: Toxoplosma gondii, 30 kDa antigen (p30), mouse, serodiagnosis, macrophage, cytotoxicity. 톡소포자충(ToxopLasmn gondii)은 다양한 항원을 가지고 있으며, 이들 항원의 분석은 세 포매개성 면역반응 및 톡소포자충증의 면역학적 진단방법의 연구에 매우 중요하다 본 연구는 톡소포자충의 여러 단백질중 대부분의 충주(strain)에 존재하는 분자량 30 kDa의 단백질을 단클론 항체를 이용하여 분리한 후. 30 kDa 항원의 면역학적 특성을 초음파 추출 조항원과 비교 평가하였다. 톡소포자충의 세포막 항원으로 면역한 마우스 비장세포와 마우스 Sp2/0-Agl4 골수종세포를 융합하여 8개의 단클론 항체를 Western blot으로 확인하였다 이들 단클론 항체는 높은 특이성을 보였으며, $IgG_{2b}가{\;}5개,{\;}IgG^{1}이{\;}2개,{\;}IgG_{2a}$가 1개였다. 간접형광항체법으로 충체내 위치를 관찰한 결과. 30 kDa 항원은 tachyzoite의 표면 세포막에 주로 분포하였다. 단클론 항체와 CNBr-activated Sepharose 4B를 coupling하여 만든 immunoafrnity chromatography를 이용 하여 30 kDa 항원을 분리하였다. 분리한 30 kDa 항원으로 자극시킨 마우스 복강대식세포의 $NO_2^{-}$ 생산량은 초음파 추출 조항원 사용군에 비해 유의하게 증가하였으나 대식세포의 탐식능은 유의한 차이가 없었다. 또한 ELISA로 톡소포자충증을 진단시, 톡소포자충 30 kDa 항원 사용군은 조항원 사용군에 비해 민감도의 변화는 없었으나 특이성은 증가하였다 이상으로 보아 톡소포자충 30 kDa 항원은 감염 방어 면역 효과가 있었으며 진단에 이용시 특이성을 더 높일 수 있었다.
白花蛇舌草 메탄올 抽出物의 抗腫瘍 效果 및 抗癌 棋戰에 關한 硏究
魯勳政,文九,文錫哉,元秦熹,文永昊,朴來佶 대한한방종양학회 2000 대한한방종양학회지 Vol.6 No.1
Objectives: This experimental study was carried out to evaluate the effects of aqueous and methanol extracts of Hedyotis diffusa which has long been used for cancer treatment in oriental medicines on the induction of apoptotic cell death in human lymphoid leukemia cell line, HL-60. Methods: Cells were treated with various concentrations (200 to 0.4㎍) and periods (6 to 30 hr) of H_2O and methanol extracts of Hedyotis diffusa. Then, cells were tested for viability by MTT assay. Cells wrere treated with 200㎍/ml of methanol extract fork various periods. Genomic DNA was isolated, separated, on 1.5% agarose gels, stained with ethidium bromide and visualized under UV light. Cells were treated with 200㎍/ml of each extract for 16hr.Then, cells were treated with various doses of each extract for 12 hr and 100㎍/ml of methanol extract for various periods. Lysate from the cells used to measure the activity of caspase-1 and-3 proteases by using fluorogenic peptide substrates including acetyl-YVAD-AMC and acetyl-DEVD-AMC, respectively, Cells were treated with 200㎍/ml of each extract for various periods. Cell lysates were immunoprecipated with anti-JNKl antibodies. The immune complex was reacted with 32^p-ATP and c-Jun as a substrate. The phosphotransferase activity of JNKI was measured by suing Phosphoimage analyzer (Fuji Co., Japan). Nuclear extracts were isolated and incubated with oligonucleotide probe of NF-kB was measured by using EMSA and visualized by PhosphoImage analyzer (Fuji Co, Japan). Cell lysates were prepared and analyzed by Western blotting with anti-Bc12 antibodies and anti-Bax antibodies. Cells were pretreated with various doses of methanol extract for 2 hr. Then, the extract was removed by centrifugation. Cells were resuspended with RPMI-1640 media containing 0.3% agarose, 10% FBS, overlayred onto bottom layer agarose and incubated at CO_2 incubator for 6 days. The number of colony was cunted under light microscopy (×100). Results: The death of HL_60 cells was markedly induced by the addition of methanol extract of Hedyotis diffusa in a dose and time-dependent manners. The apoptotic characteristic ladder pattern of DNA strand break was observed in death of HL_60 cells. In addition, it was shown nucleus chromatin condensation under Hoechst staining. Therefore, Hedyotis diffusa extract-induced death of HL-60 cells is mediated by apoptotic signaling processes. The activity of Caspaxe 3-like proteases remained in a basal level in HL-60 cells treated with aqueous extract of Hedyotis diffusa. However, it was markedly increased in HL-60 cells treated with methanol extract of Hedyotis diffusa. In addition, the phosphotransferase activity of JNKl was increased in HL-60 cells treated with methanol extract of Hedyotis diffusa. Furthermore, the activation of transcriptional activator, NF-kB was markedly induced by methanol extract of Hedyotis diffusa. Anti-apoptotic Bc12 was cleaved into 23Kda fragment by treatment of methanol extract of Hedyotis diffusa. However, expression of proapoptotic Bax protein was increased by treatment of methanol extract of Hedyotis diffusa in a time-dependent manner. Furthermore, methanol extract markedly inhibited the colony forming efficiency of HL-60 cells in semisolid agar culture. Conclusions: Above results suggest that methanol extract of Hedyotis diffusa induces the apoptotic death of human leukemic HL-60 cells via activations of Caspase-3 proteases, JNKI, transcriptional activator NF-κB, In addition, our results also suggest that methanol exthanol extract of Hedyotis diffusa reduces the malignant potential of HL-60 cells via down regulation of colony forming effciency through cleavage of Bc12 as well as induction of Bax.
개선된 불요응답 특성을 갖는 DGS형태의 CPW 저역통과 여파기의 설계
노진원,황희용 강원대학교 정보통신연구소 2006 정보통신논문지 Vol.10 No.-
In this paper, Coplanar Waveguide(CPW) low-pass filter with the spurious-reduced characteristic is designed by using Defected Ground Structures(DGS). Two different LPF unit cells and DGS Pattern are Combined to reduce the spurious. In comparison to a conventional step impedance LPF, the size of proposed LPF as 17.2 mm × 6.8 mm is serveral times smaller. The simulation and measurements confirm that spurious characteristics is presented less than -30dB(S21) at the three times range of cut off frequency.
魯津生,李哲遠 公州大學校 스포츠科學硏究所 1996 스포츠科學硏究所論文集 Vol.- No.10
I'll explain the types and traits of education policy, the basic charicteristics of physical education policy, the nature of imperialistic Japan through the suppress of nationalistic physical education, and the inevitable irrationality of colonial policy, dividing colonial policy with had been executed in the field of education, economy, physical education of our country for thirty-six years from 1910, the year of Korea-Japan annexation into three period(the period of military control, the period of cultural politics, and the period of race obliteration). 1. The Period of Military Control On August 23, 1911, the next year after annexation, Japan pronounced the Decree of Chosun Education and with the aim of raising faithful people carried out race policies discriminating Koreans from Japanese in the curriculum and education system, and spurred the spread of their own language, Japanese. Physical education of this period was the obligatory course, bur nit can not be evaluated as development to pay considerable attention to sanitation and introduce physical activities into everyday life. It was not accomplished independently by the earnest demands of our nation, and was processed by the Japanese' colonial persons in authorities under the name of modernization. At that time, there were not the given class hours and the contents in physical education, so they could be handled freely by the local problems and were neglected intentionally. We can imagine .that Japanese were afraid of race unity through physical education. School physical education of the late Old Korea encouraged the patriot of the young domestically through army gymnastics, and showed the national power abroad. Japan noticed that the physical education of the private school encouraged the anti-Japanese sentiment. So Japan changed the contents of physical education into ordinary gymnastics and heavy gymnastics in 1911 to soften the anti-Japanese sentiment. Japan used physical education as the tools of mobocracy policy to cultivate the faithful colonial people. 2. The Period of Cultural Politics The Second Decree of Chosun Education in 1922 lengthened the school years and increased the education, and school Physical Education and Chosun Physical Education Association play the important role. In the period, inter-school games were prevailed a lot, and thus school physical education took charge of social education. Since 1920's, school physical education has meant pure-sensed one, in other words, physical education was taught to students according to the curriculum of physical education, and social physical education, in which each school raised its own team and played with other teams, strictly speaking, is beyond the school physical education. At that time most of school physical education games were field and track events, baseball, and basketball. With all the lack of sports equipment, people loved to play seasonal sports. The basic policy of physical education was children and interest - centered education based on the curriculum of school physical education revised in 1927. 3. The Period of Race Obliteration In this period, education was completely organized under the control of army which is called the fascism - a kind of militarism, according to the Third Decree of Chosun education and was directed to growing of military ability rather than education. This period was the dark age in physical education. Japan pronounced reforming guiding principles of school physical education on April 18, 1942 and decided the basic direction of physical education. They also abolished ball games in college and university, and restricted inter-school ball games in secondary schools. So physical games had only the meaning of examination of physical strength for military training. These policies of Japan made girl students in all schools be treated as military men to accomplish the aim of war. The basic policies of physical education in late colonial period had not original aimes of physical education in itself, but focussed on raising the ability of war performance of Japanese militarism. These policies abolished the independence and Olympic spirits which are the guiding principles of physical education world and planted the totalitarian, militarian physical education in Korea.
정평진,박노훈 단국대학교 1998 論文集 Vol.33 No.-
The Nalkylcarbazole polymers were synthesized by cationic polymerization. These polymerizations were reacted under different condition for 3hrs or 24 hrs. These reactions between Nunsaturatedalkylcarbazole monomers and boron trifluoride diethyl etherate(BF_3ㆍEt_2O) were conducted in toluene at ice water bath under N_2 atmosphere or in benzene at room temperature under vacuum atmosphere. At htis time the starting materials were monomer 1.0g and initiator 1.42㎎, respectively. All of these compounds of substrates and products were characterized by melting point. FTIR and ^1HNMR spectroscopy.
천연제올라이트를 지지체로 사용한 고온건식 아연계 탈황제의 반응특성 연구
박노국,정용길,이종대,이태진,김재창 한국화학공학회 2003 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.41 No.5
고온건식 탈황제의 지지체로 알루미나와 천연제올라이트를 사용하여 아연계 탈황제를 제조하였으며 성능을 조사하였다. 두 가지 탈황제의 반응특성실험은 480℃/580℃(황화/재생온도)에서 칸 밸런스와 고정층 반응장치로 반응속도와 황수용능력을 비교하였으며 내마모 실험장치를 이용하여 탈황제의 내마모도를 조사하였다. 두 탈황제의 성능 실험결과 천연제올라이트를 지지체로 사용한 탈황제의 초기 황화반응속도가 알루미나를 지지체로 사용한 탈황제보다 빨라졌으며, 10 cycle에서 황수용능력이 20 gS/100 g sorbent 이상 유지되었다. 내마모성은 AI(마모지표)값이 14.7%정도였다. 본 연구에서는 천연제올라이트의 고온건식 탈황제의 지지체로 적용 가능성을 확인하였다. Two types of zinc-based sorbents using alumina and natural zeolite as the supports for hot-gas desulfurization were prepared, and investigated their desulfurization capability. Their reaction rate and sulfur capacity were compared by Cahn balance and over the fixed bed reactor system at 480℃/580℃ (sulfidation/regeneration). The attrition resistance was measured by ASTM methd. Thc initial sulfidation rate of ZnO/natural zeolite sorbent was higher than that of ZnO/alumina, and the sulfur capacity of ZnO/natural zeolite sorbent was maintained above 20 gS/l00 g sorbent for 10 cycles. A attrition index was 14.7%. The use of natural zeolite as a support of sorbents may be possible for hot gas desulfurization.