Inhibition of oxidative stress induced‑cytotoxicity by coptisine in V79‑4 Chinese hamster lung fibroblasts through the induction of Nrf‑2 mediated HO‑1 expression

Hyeon‑Gyun Jo,Cheol Park,이혜숙,Gi‑Young Kim,Young‑Sam Keum,Jin Won Hyun,Taeg Kyu Kwon,Yung Hyun Choi,Su Hyun Hong 한국유전학회 2021 Genes & Genomics Vol.43 No.1

Background Coptisine is a natural alkaloid compound and is known to have multiple benefcial efects including antioxidant activity. However, whether it can protect lung fbroblasts from oxidative damage has not been studied yet. Objectives To investigate the potential inhibitory efect of coptisine against oxidative stress in V79-4 lung fbroblast cells. Methods V79-4 cells were treated with H2O2 (1 mM) in the presence or absence of coptisine (50 µg/ml), N-acetyl cysteine (NAC, 10 mM) or zinc protoporphyrin IX (ZnPP, 10 µM) for the indicated times. The alleviating efects of coptisine on cytotoxicity, cell cycle arrest, apoptosis, reactive oxygen species (ROS) production, DNA damage, mitochondrial dynamics, and inhibition of ATP production against H2O2 were investigated. Western blot analysis was used to analyze the expression levels of specifc proteins. Results Coptisine inhibited H2O2-induced cytotoxicity and DNA damage by blocking abnormal ROS generation. H2O2 treatment caused cell cycle arrest at the G2/M phase accompanied by increased expression of cyclin-dependent kinase (Cdk) inhibitor p21WAF1/CIP1 and decreased expression of cyclin B1 and cyclin A. However, these efects were attenuated in the presence of coptisine or NAC. Coptisine also prevented apoptosis by decreasing the rate of Bax/Bcl-2 expression in H2O2-stimulated cells and suppressing the loss of mitochondrial membrane potential and the cytosolic release of cytochrome c. In addition, the activation of nuclear factor-erythroid-2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) was markedly promoted by coptisine in the presence of H2O2. However, zinc protoporphyrin IX, a potent inhibitor of HO-1, attenuated the ROS scavenging and anti-apoptotic efects of coptisine. Conclusions Based on current data, we suggest that coptisine can be used as a potential treatment for oxidative stress-related lung disease.

진주지역 제조기업의 산업재해 실태분석과 대책방안

최진현,강호욱 경상대학교 경남문화연구소 1983 慶南文化硏究 Vol.6 No.1

The process of manufacturing enterprise takes the potential injury agents (moving equipment, machines and tools, hazards, etc). The industrial accidents due to these occasion happen unexpectedly. Safety management, in the manufacturing enterprise, are a moral action for worker, and improve the production efficiency and decrease the accident cost. In this study, industrial accidents are analyzed on the actual condition for 339 manufacturing enterprise, and select the 9 sampling enterprise. We simulate the relationship between accident cost and safety management cost. So, We suggest the safety management counterplan. The actual condition of the industrial accidents were analyzed by I.L.O. statistical analysis equations, which are expressed by (1) and (2). FR=N/H×10^6 …(1) SR=S/H×10³ …(2) Logical development of the alternatives for safety management counterplan based on the structure of industrial accident. We derived characteritics of the industrial accidents, and suggested a few counterplan of industrial accidents for manafacturing enterprises in Jin-Ju area.

휴대용 핵종 분석기의 유용성 평가

김지현(Ji Hyeon Kim),전성진(Seong Jin Jin),제재용(Jae Yong Je) 한국방사선학회 2015 한국방사선학회 학술대회 논문집 Vol.2015 No.춘계

본 연구는 휴대용 핵종분석기를 이용하여 부산지역의 지역별 환경방사선을 측정하여 휴개용 핵종분석기의 유용성을 평가하고자 한다. 측정 대상은 부산의 중심에 위치한 부산진구 소재 송상현 광장을 중심으로 9곳의 환경 방사선을 측정하였다. 측정결과 부산역과 부산대학교를 제외한 대부분의 지역에서 환경방사선은 비슷한 결과를 나탄내었다. 검출된 핵종은241Am, 133Ba, 54Mn등이 검출되었고 핵종분석기에 내장된 핵종정보가 부족하여 알 수 없는 핵종들도 검출되었다. 실험에 사용한 휴대용 핵종분석기는 분해능이 8% 정도로 자연방사성 핵종과 인공방사성 핵종을 핵종별로 정확하게 분석하기 위해서는 제조사의 개선이 필요한 것으로 판단된다. This research aims at measuring the environmental radioactivity at each area of Busan using a potable radio-isotope identification system, and assessing the utility of the potable radio-iosotope identification system. As for the target for measurement, the environmental radioactivities of nine areas centered around Songsanghyeon Square in Jin-gu located in the center of Busan were measured. As the measurement result, the environmental radioactivities were similar in most of the areas except in Busan station and Busan University. The nuclides, such as 241Am, 133Ba, 54Mn and so on, were detected, and some nuclides whose identity was unknown because of lack of information built in the radiation spectrum analyzer were also found. The potable radio-isotope identification system used in the experiment had about 8% of resolving power, so for more precise analysis into the nuclides of natural background radiation and artificial radiation in each type, the manufacturer needs to improve the analyzer.

올레산의 비율에 따른 아데노신의 경피 투과의 영향

남현빈,정세진,구민석,오예진,김용민,이훈희,윤수정,임정균 순천향대학교 부설 산업기술연구소 2021 순천향 산업기술연구소논문집 Vol.27 No.2

In addition to the development of functional cosmetics, technology to increase percutaneous absorption rate when applied to the skin is an important task. In this experiment, oleic acid was used as a skin absorption promoter, and its effect on transdermal permeability of adenosine was studied and analyzed using a skin-like membrane. As a result of the experiment, it was investigated that the permeability coefficient and concentration of adenosine are proportional to the content of oleic acid. This indicates that a high proportion of oleic acid is effective as a skin absorption promoter.

진행성 폐 종양 환자에서 종양 내 OK-432 주입술의 효과

정수현,김성빈,박진희,탁희상,옥철호,장태원,정만홍,천봉권 고신대학교 의과대학 2009 고신대학교 의과대학 학술지 Vol.24 No.2

Background: Intratumoral injection of OK-432 produced significant antitumor effects. There are reports that OK-432 was injected directly into tumor tissue on gastrointestinal tracts and HCC tissue, producing decrease in serum alpha-fetoprotein level. We adopted this technique for treating locally advanced lung cancer. The object of this study is to evaluate the local therapeutic efficacy, side effects of injection for treating symptomatic mass. Methods: Twenty lung cancer patients (13 males and 7 females) underwent sonography guided OK-432 injection. We analyzed relationships between response of OK-432 and response of skin test, histology and age. Pain that patients feel was evaluated by VAS (Visual Analog scale). Results: A significant decrease of tumor size was observed. The mean pre-injection diameter of the lesions was 54.7±11.3mm, PR rate were 15.0% (3/20) and minimal change (50%<tumor size decreased.) patients were 35.0% (7/20). The VAS score before injecting OK-432 into the lesion was 5±3, this was decreased to 2.5±1.5 (p=0.04). Object response was significantly correlated with skin response, adenocarcinoma, and age. Conclusion: A significant decrease of tumor size and pain was observed. So OK-432 is a valuable treatment option for patients with symptomatic lesion from advanced lung cancer who are not amenable to further therapy.

WIPI 기반 모바일 3D 게임엔진의 설계와 구현

형대진,김승구,조현준,박경환 동아대학교 정보기술연구소 2006 情報技術硏究所論文誌 Vol.14 No.1

In this paper, we introduce the method for developing the mobile 3D game engine based on the WIPI platform which has been proposed as the mobile standard platform. Our game engine is designed so that it supports OpenGL-ES and JSR-184 that are mobile 3D graphic APIs. Our game engine has a mobile emulator operated on PC and SDK to support mobile programming in PC environments. Mobile 3D game developers can use the APIs at various levels. Also, the game engine is developed in modular in order to support various game genres.

Continuous wave of obturation technique에서 플러거의 다양한 적용 깊이에 따른 근단부 폐쇄효율 비교

이상진,박정길,허복,김현철 大韓齒科保存學會 2007 Restorative Dentistry & Endodontics Vol.32 No.6

이 연구와 목적은 continuous wave of obturation technique으로 근관 충전을 할 때, 다양한 heat source plugger의 깊이에 따른 근단부 근관 폐쇄능력을 비교하는 것이었다. 직전 형태 발거 소구치를 #35/.06크기까지 니켈티타늄 파일을 사용하여 성형 및 세정하였다. 성형과 세정이 완료된 치아를 heat source plugger의 적용 깊이에 따라 15개씩 5군으로 분류하였다: 3 mm, 4 mm, 5 mm, 6 mm, 7 mm. 양성 대조군을 제외한 모든 근관은 E&Q plus (Meta Biomed Co., Chungju, Korea)를 사용하여 #35/.06 gutta-percha cone으로 충전하였다. 모든 치아의 근첨공 주변 1 mm를 제외하고 nail varnish를 도포하였고 음성 대조군은 근첨공을 포함하여 전체 면을 도포하였다. 모든 시편을 1% 메틸렌블루 용액에 72시간 동안 보관한 다음 치근첨으로부터 1, 2, 3 mm 높이에서 수평으로 절단하였다. 각 단면을 12.5 x 2.5배 현미경하에서 관찰하고 디지털 카메라로 촬영하였다. 염색제 누출 평가를 위해 five-degree scoring system을 사용하였다. 각 절단면에서의 점수를 모두 합하여 한 시편의 점수로 사용하고 통계학적 분석을 하였다. 이 실험 조건하에서, 3 - 7 mm 까지의 heat source plugger의 적용 깊이는 근단부 폐쇄에서 유의한 차이를 나타내지 않았다. 5 - 7 mm의 Buchanan's protocol을 포함한 3 - 7 mm를 모두 임상 적용에서 받아들여질 만하다고 보인다. The purpose of this study was to compare apical sealing ability of continuous wave canal filling technique according to various heat source plugging depths. Eighty one extracted human premolars with straight root were cleaned and shaped to size 35 using .06 taper rotaiy NiTi file. After cleansing and shaping, the teeth were divided into 5 groups following the heat source probing depths from the apex ; 3, 4, 5, 6 and 7 mm. All specimens were filled using E&Q Plus with #35/.06 tapered gutta-percha cone. The positive control teeth were not filled. All teeth were coated with nail varnish except the apical 1 mm around the apical foramen. Negative control teeth were completely sealed include the apical foramen. All specimens were immersed in 1% methylene blue solution for 72 hours. Then the specimens were sectioned horizontally at 1, 2 and 3 mm from the root apex. Each sectioned surface was photographed using a digital camera attached to the stereomicroscope at 12.5 x 2.5 fold magnification. All points at 1, 2 and 3 mm were summed as final score of one specimen. Statistical analysis of the collected data was performed. Under the condition of this study, there was no significant difference between the heat source plugging depths of 3, 4, 5, 6 and 7 mm in apical sealing ability. All of apical heat source plugging depth from 3 to 7 mm including Buchanan's protocol -from 5 to 7 mm- seems to be acceptable in clinical application.

君笭湯이 장기 알콜투여로 손상된 생쥐 간조직 회복에 미치는 영향

김호현,강윤호,안상현,박인식,이해풍,김진택 世明대학교 한의학연구소 1999 韓醫學硏究所 論文集 Vol.2 No.-

Long-term alcohol consumption have significant harmful effects as fatty liver, hepatitis, and cirrhosis on the hepatic tissues. After voluntary administration of 8% alcohol(18.67㎖/kg/day) for 120 days, the hepatic tissues of ICR mice were daministered with Kunryeongtang extract(KRE;1.24g/kg/day) were observed to investigate the repaire effects of KRE for hepatic damages that caused by chronic alcohol administration. In chronic alcohol administered group, the vacuolation such as fat accumulation(steatosis) were increased in cytoplasm, the destructive configuration of liver plates were shown in necrotic lesion. These regions are aggregation of inflammatory component cell as lymphocytes, neutrophil leucocytes and Kuffer's cell. These hepatic damages were aggravated with time course. In KRE treated group, the decreased of vacuolation and normal construction of liver plates were appeared. These results indicated that the KRE work on the repair of hepatic damages induced chronic alcohol administration.

센서 노드의 프로그래밍 환경 비교

윤현주,오병진 금오공과대학교 2006 論文集 Vol.27 No.-

Software development environment is one of important features to extend the application area and to make rapid progress in any computing application fields. We studied and compared the programming environments for wireless sensor networks, which becomes an essential technology towards the ubiquitous era. Two representative environments, TinyOS/nesC and Nano Qplus/C were investigated and the comparison was performed by implementing a typical application including sensing and multi - hop transmission. We experienced and observed that the TinyOS/nesC environment is difficult at initial stage due to the unfamiliar programming model, but the result codes are more compact and have abundant reference implementations. On the other side, Nano Qplus/C environment is easy to adaptable for general purpose programmers and provides more features such as multi-threading and dynamic memory allocation.

전기도금을 이용한 스테인레스 스틸 선재의 직경 증가에 관한 실험적 연구

조진형,성영은,이기헌,황현식 대한치과교정학회 2003 대한치과교정학회지 Vol.33 No.2

본 연구는 전기도금을 이용하여 교정용 선재의 직경을 증가시킨 후 그 물성 변화를 알아봄으로써 이의 임상적 적용 가능성을 알아보고자 시행되었다. 0.016 인치 스테인레스 스틸 교정용 선재에 전기도금을 위한 적절한 전처리를 시행한 후, 황상 니켈 100g/L, 염화니켈 60g/L, 붕산 30g/L, 염화나트륨 50g/L의 조성을 가지는 전해액을 제조하여 1.7V의 전압과 25∼29℃의 온도 3.1∼3.3pH의 조건 하에서 직경을 0.002 인치 증가시켜 0.018 인치 직경의 선재로 만들었다. 전기도금 과정 중 1분 단위로 직경을 측정하여 시간에 따른 직경 증가율을 구하였고, 도금 후 서로 다른 세 지점의 직경을 계측하여 균일성을 평가하였으며, 도금 금속의 정성분석을 위하여 X-선 회절감사를 시행하였다. 도금층의 밀착성 증진을 위해 400℃의 전기로에서 10분간 열처리를 시행한 후 도금 전후와 열처리 전후의 물성 변화를 알아보기 위하여 3점 굴곡 실험을 시행하여 다음과 같은 결과를 얻었다. 1. 전반적인 하중-변형을 곡선을 보면 0.016 인치 군과 0.018 인치 군 사이에 도금 처리한 군이 존재하였으며 열처리한 경우가 0.018 인치 군에 더욱 가까워지는 양상을 보였다. 2. 도금에 의해 직경은 0.002 인치 증가시킨 군이 기존의 0.016 인치 군에 비하여 강성과 항복상도, 극한강도 모두 증가하는 경향을 보였으며 이 중 강성과 극한 강도는 통계적인 유의한 차이를 보였다. 3. 0.016 인치에서 도금에 의해 직경을 0.002 인치 증가시킨 준이 기존의 0.018 인치 군보다 강성과 항복강도 극한강도 모두에서 낮게 나타났으며 통계적 유의차를 보았다. 4. 도금 후 열처리를 시행한 군이 시행하지 않은 군보다 강성, 항복강도, 극한강도 모두 높은 경향을 보였으며, 극한강도에서 유의한 차이를 나타내었다. 5. 전기도금 후 서로 다른 세 지점의 직경차이는 0.1∼0.3%로 균일하게 나타났으며 통계적으로도 유의차가 존재하지 않았다. The purpose of this study was to evaluate the possibile clinical application of electroplating to increase diameter of an orthodontic wire, through examining the change of physical properties. The diameter of stainless steel orthodontic wire was increased from 0.016 inch to 0.018 inch by electroplating in a bath of nickel sulfate 100g/L, nickel chloride 60g/L, boric acid 30g/L, and sodium chloride 50g/L, under the conditions of 1.7V, 25∼29℃, and 3.1∼3.3pH. During the electroplating, the rate of diameter increase was measured every minute. To investigate uniformity, the diameter was performed to analyze the nature of the electroplated metal. Following heat treatment to improve adhesion between the wire and electroplated metal, a three-point bending test was conducted to compare stiffness, yield strength, and ultimate strength among four wire groups; 0.016 inch, electroplated 016, electroplated and heat-treated 016, and 0.018 inch wires. Through the comparison of each wire group, following results were obtained. 1. In the load-deflection graph, the curve of the electroplated group was places between that of the 0.016 inch group and the 0.018 inch group, and the curve was closer to the 0.018 inch group by heat treatment. 2. In the electroplated and heat-treated 016 wire group, the values of stiffness, yield strength and ultimate strength showed higher tendency than in the original 0.016 inch group. Stiffness and ultimate strength showed statistically significant differences between two groups. 3. Stiffness, yield strength, and ultimate strength of electroplated wire presented lower values than those of 0.018 inch wire group. 4. Stiffness, yield strength, and ultimate strength of electroplated and heat-treated wire showed higher tendency than those of electroplated wire group, and ultimate strength showed statistically significant difference between two groups. 5. After electroplating, the difference in diameter between the three locations was within 0.1∼0.3% variation, and showed no statistical significance.