MicroRNA-362 Inhibits Cell Proliferation and Invasion by Directly Targeting SIX1 in Colorectal Cancer

Jin’e Wan,Jian Yang,Cuixia Qiao,Xiaomei Sun,Aiting Di,Lize Zhang,Dandan Wang,Gang Zhao 연세대학교의과대학 2019 Yonsei medical journal Vol.60 No.5

Purpose: Colorectal cancer (CRC) is the third most common cancer in China and poses high morbidity and mortality. In recentyears, increasing evidence has indicated that microRNAs played important functions in the occurrence and development of tumors. The purpose of this study was to identify the biological mechanisms of miR-362 in CRC. Materials and Methods: Quantitative real-time PCR was carried out to assess the expression of miR-362 and SIX1. The Kaplan-Meier method was employed to evaluate the 5-year overall survival of CRC patients. The proliferative and invasive abilities of CRCcells were assessed by MTT and transwell assays. Results: miR-362 was significantly decreased in CRC tissues and cell lines, compared to the normal tissues and normal cells. Asignificant connection was confirmed between the overall survival of 53 CRC patients and low expression of miR-362. Downregulationof miR-362 inhibited the proliferation and invasion through binding to the 3'-UTR of SIX1 mRNA in CRC. Additionally, wediscovered that SIX1 was a direct target gene of miR-362 and that the expression of miR-362 had a negative connection with SIX1expression in CRC. SIX1 could reverse partial functions in the proliferation and invasion in CRC cells. Conclusion: miR-362 may be a prognostic marker in CRC and suppress CRC cell proliferation and invasion in part through targetingthe 3'-UTR of SIX1 mRNA. The newly identified miR-362/SIX1 axis provides insight into the progression of CRC.

Dislocation Density-Based Constitutive Model and Processing Map for T2 Copper During Isothermal and Time-Variant Deformation

Gang Chen,Yu Jin,Jing Wang,Cheng Zhang,Qiang Chen,Hongming Zhang,Xingjian Zhao,Zhiyong Li,Changhai Xie,Zhiming Du 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.9

Isothermal compression tests were carried out to study the hot deformation behavior of T2 copper under various conditions. The deformation parameters, such as temperature and strain rate, have strong influence on flow stress and microstructureevolution of the alloy. A unified dislocation density-based model considering dynamic recovery and recrystallization wasestablished. And material parameters of the developed model were optimized by genetic algorithm. Comparisons betweenthe experimental and model data demonstrates that the developed model can precisely describe the flow behavior at quitewide range of deformation conditions. Meanwhile, the designed iterative procedure allows the model to be applied in timevariantdeformation conditions. Processing map and microstructure examination were constructed to optimize the processingwindow of the studied alloy. According to the processing maps, flow instability mainly appeared at low temperatures of500–650 °C and strain rates higher than 0.1 s−1. The optimum deformation parameters of T2 copper was concluded as thetemperature range of 700–800 °C and the strain rate of 0.1–1 s−1.

농업유역 소하천의 T-P,T-N 모니터링

이강석,연규방,박진기,나상일,박종화 충북대학교 농업과학기술연구소 2012 農業科學硏究 Vol.28 No.1

본문참고

이동 소하천의 수온,pH,부유물질량,용존산소,생화학적 산소요구량의 특성

이간석,연규방,박진기,나상일,박종화 충북대학교 농업과학기술연구소 2012 農業科學硏究 Vol.28 No.1

본문참고

Lipopolysaccharide 로 유발된 생쥐 무릎관절낭 염증에 관한 형태학적 연구 : 윤활관절막과 섬유관절막의 변화를 중심으로 Based on the Morphological Changes of Synovial Membrane and Fibrous Membrane

김진택,안상현,최난희,정재만,박인식,강윤호,김호현,이해풍 동국대학교 한의학연구소 1999 東國韓醫學硏究所論文集 Vol.7 No.2

본 연구는 관절염 유발시 일어나는 관절낭의 형태학적 변화를 조사하기위해 ljpopolysaccharide(LPS)주사로 인위적 관절낭 염증을 유발시킨 후 시간경과에 따른 윤활관절막과 섬유관절막의 형태 변화를 관찰하였다. BALB/C 암컷 생쥐 오른쪽 무릎관절낭에 LPS 300㎍/㎏를 주사한 후 3, 7 그리고 14일에 무릎관절을 얻었다. 무릎관절은 4주동안 EDTA용액에 탈회한 후 통상적 방법으로 paraffin에 포매하였다. 또한 윤활관절막의 미세구조변화는 embed812로 포매한 후 관찰하였다. LPS 주사후 관절연골 인접부위의 윤활관절막에서 시작된 세포과형성(hyperplasia)은 시간 경과후 전체 윤활관절막으로 확대되었다. 윤활관절막내의 미세구조의 변화로는 윤활포식세포(type 1)가 관절강내로의 많은 돌기(filopodia)를 내었고, 잘 발달된 과립형질내세망을 가지는 type 2 윤활분비세포의 숫적 증가가 보였다. 한편 LPS 주사후 섬유관절막에서 나타나는 형태학적 변화는 collagen fiber 생성에 의한 섬유화가 증가되며, 이러한 섬유화를 주도하는 섬유모세포의 이주증가파 관찰되었다. 또한 혈관 주위에서는 백혈구의 이주 증가가 나타났으며, 탈과립형(degranulated type) 비만세포가 많이 관찰되었다. 이상의 결과로 LPS 주사로 관절낭에서 염증이 유발되어 윤활관전막과 섬유관절막에서 형태학적 변화가 나타났다. 이러한 일련의 형태학적 변화는 발병초기 류마티스성 관절염에서 나타나는 병리학적 소건과 동일한 결과로서, 앞으로 진행될 치료제 개발과 유발기전에 관한 해석을 위한 in vivo 실험의 적절한 모델로 기여한 것으로 기대된다. Synovial joint of BALB/C mice were injeced with Lipopolysaccharide(LPS) were observed to investigate the morphological changes of synovial capsule caused by rheumatoid arthritis(RA). The RA on female Balb/c mice were induced by LPS injection, as dose of 300㎍/㎏, into synovial cavity of knee joint. And then these specimen were fixed in 10% neutral buffered formalin and were decalcificated in EDTA solution for 4 weeks. The hyperplasia of synovium were appeared in synovial membrane. The filopodia of phagocytic like synoviocyte(type Ⅰ synoviocyte) projected into synovial cavity and the number of fibroblast like synoviocyte(type Ⅱ synoviocyte) with well-developed endoplasmic reticulum were increased in synovium. In fibrous membrane, the fibrosis induced by synthesis of collagen fiber were enlarged to all fibrous membrane, and the number of fibroblast were increased. A great number of inflammation component cell as Iymphocyte and neutrophil leukocyte were infiltrated around capillary and the degranulate typed mast cell were increased. As results indicated that the hyperplasia of synovium induced by LPS, subsequently to cause the fibrosis, infiltration of imflammation component cell, and increase of degranulated type mast call as same as symptoms of RA.

BSC 지표들을 이용한 ERP시스템의 성과 측정

김강진,신춘우,김기평 朝鮮大學校 經營經濟硏究所 2007 經營經濟硏究 = Management and economics research Vol.30 No.1

동진강 유역내 하천의 특성별 영향평가

윤순강,김원일,김진호,김선종,고문환,엄기철 한국환경농학회 2002 한국환경농학회지 Vol.21 No.4

본 연구는 동진강 유역의 수질 보전을 위한 기초자료를 얻기 위하여 동진강 본류와 이에 유입되는 정읍천을 대상으로 2001년 5월부터 11월까지 수질 모니터링을 실시하였다. 동진강 본류의 수질은 BOD기준으로 상수원 Ⅱ~Ⅳ등급(2.84~6.45 ㎎/L)으로 지점별로는 DJ6(정읍천 합류후)이 4.07㎎/L로 높게 나타나 Ⅲ급수의 수질을 유지하였다. COD는 지점별로 11.20~32.96 ㎎/L의 범위로 정읍천 합류후의 농도가 32.96 ㎎/L로 가장 높게 나타나 정읍천을 통한 본류의 오염물질량의 증가를 알 수 있었다. T-N의 경우 4.16~5.84 ㎎/L의 범위로 유역 전반에 걸쳐 오염이 이루어지고 있는 것으로 나타났고, T-P의 경우 BOD, COD와 마찬가지로 정읍천 합류후가 0.19 ㎎/L로 타 지점에 비하여 놀게 조사되었다. 본류의 COD의 경우 장마후 갈수기에 높은 농도를 나타냈고, T-P의 경우는 하천의 유량이 증가하는 홍수기보다 장마전 갈수기의 오염정도가 높고 이후 장마후 갈수기로 갈수록 수질이 악화되는 것으로 조사되었다. 동진강 수질의 오염은 주로 하류로 갈수록 그 정도가 심하게 나타나는데, 이것은 정읍천을 통해 유입되는 도시 생활하수와 인근 공장단지의 산업폐수가 그 주 요인으로 오염물질(BOD, COD T-N 및 T-P)의 농도가 상승하는 주원인이 되고 있다. 따라서, 동진강 수계의 수질관리를 위해 주 오염원이 되고 있는 정읍천의 도시생활하수와 공장단지에서 방류되는 산업폐수의 제어에 대한 대책이 먼저 이루어져야 할 것으로 판단된다. Irrigation water quality along Donjin river watershed was monitored to find a possible pollutants, for maintaining water quality to achieve food safety through water quality preservation of river. As a pollution indicators, such as Biological Oxygen Demand(BOD), Chemical Oxygen Demand(COD), Total Nitrogen(T-N), and Total Phosphate(T-P) in Dongjin river were examined from May to November in 2001. The results were as follows : The BOD level of Dongji n river ranged from 2.84 to 6.45 mg/L, which would be in a Ⅱ~Ⅳ grade of the potable water criteria by Ministry of Environment. Averaged BOD level of downstream DJ6(After Jeongupcheon confluence) was 4.07 mg/L. The average COD level of Dongjin river ranged from 11.20 to 32.96 mg/L. COD level of DJ6 rapidly increased rapidly after the junction of Dongjin river and Jungupcheon because it showed the latter had relatively high pollution level. T-N content were significantly high in all sites of Dongjin river ranged through 4.16 to 5.84 mg/L. T-P examined high concentration than another thing point by 0.19 mg/L after Jeongupcheon confluence as BOD and COD. COD of main stream was expressed high concentration to dry season after rainy season. In case of T-P, pollution degree of dry season before rainy season appeared and examined that quality of water was worsened go by dry season after rainy season. The water quality of Dongjin river was deteriorated with inflow of Jungupcheon polluted by municipal and industrial sites near Jungup city.

우리나라 중부지방의 시설원에 토양 및 지하수 환경

김진호,이종식,김원일,정구복,윤순강,정연태,권순국 한국환경농학회 2002 한국환경농학회지 Vol.21 No.2

연구대상 시설재배지 토양의 물리성은 대부분이 모래의 비율이 높은 sandy loam이나 loam으로 투수성이 양호할 것으로 판단되나, P2나 S1에서는 미사의 비율이 높은 미사 loam으로 투수성 및 통기성이 상대적으로 낮은 것으로 판단된다. 토양의 화학성은 EC의 경우 표토의 대부분이 기준치에 도달하거나, 초과하는 경향이었고, 심토의 경우는 기준치 이하로 조사되었다. 또한 유기물 함량은 적정수준 2~3%보다 조사 대상 지점의 대부분에서 상회하였고, 심토의 일부분도 이 수준을 넘는 곳도 있었다. 조사 대상 지역의 수질 특성 중 시설재배지 지하수의 가장 중요한 염류 지표인 EC는 연평균 0.48 dS/m로 농업용수로서 작물에 미치는 영향은 없는 것으로 판단되나, 수질오염지표인 질산성 질소의 경우 19.1 mg/L 로 농업용수 수질 기준인 20 mg/L에 근접한 수준을 나타내었다. 특히 조사 대상 지점의 36.4%가 수질 기준을 초과하는 것으로 나타났다. 지하수중의 이온의 양과 영양염류의 양을 간접적으로 나타내는 전기전도도인 EC는 양이온인 경우 Mg^(2+), Ca^(2+), Na^+, K^+와 정의상관이 있었으며, 특히 2가 양이온인 Mg^(2+)와 Ca^(2+)간에는 고도의 정의상관을 보이고 있었다. 또한 EC와 음이온간에는 NO_3^-, Cl^-, SO_4^(2-), PO_4^(2-)와 상관을 보이고 있었다. 지하수의 오염지표인 NO_3-N의 경우에는 COD를 제외한 모든 이온들과 상관을 보이고 있었다. 특히 SO_4^(2-), Cl^-, Na^+, Ca^(2+), Mg^(2+)와는 고도의 정의 상관을 보여주고 있다. 이는 NO_3-N는 EC와 함께 지하수오염의 중요한 지표중의 하나임을 보여주고 있다. The objective of this study was to know the qualities of soil and shallow groundwater in plastic film house fields around Central Part of Korea. The study was conducted at 11 sites in Suweon, Hwasung, Pyungtaek, Yongin, and Chuncheon through May to August in 1999. Soil textures of plastic films house were mainly sandy loam or loam. Electric conductivity and organic matter content of surface soils mostly exceeded the critical levels for crop production. Average concentration of N0_3-N in the shallow groundwater was 19.1 mg/L, and it reached almost the limiting level of agricultural groundwater quality (20 mg/L). Moreover about 36% of survey sites exceeded the limiting level of agricultural groundwater quality. Sulfate concentrations also at some sites exceeded agricultural groundwater quality limit level (50 mg/L). Nitrate-N, one of the most important factors in the groundwater quality, had positive correlations with other ions in groundwater.

석고판 경량 간막이 벽체의 구성방법에 따른 차음성능에 관한 연구

이태강,정진연,김선우 대한건축학회 2005 대한건축학회논문집 Vol.21 No.2

This paper aims to investigate the characteristics of partition walls which are light weighted, dry walls of gypsum boards, These partition walls are prospected to be new ones to cope with M.C.(Modular Coordination), flexibility, non-bearing system, substitution to masonry works. The number of tested partition walls are 16 which are varied with core materials, depth and construction methods. These partitions of sound insulation characteristics of light weighted partitions were tested in reverberation room. And compared with 0.5B and 1B masonry wall of sound insulation performance, the partitions are evaluated by R_w performance. The sound insulation performance of the gypsum board partitions are as good as the performance of masonry wall.

비디오 흉강경을 이용한 자연성 기흉 수술에서 재발 방지 방법에 대한 연구

박성강,이석기,임진수,최형호 朝鮮大學校 附設 醫學硏究所 2004 The Medical Journal of Chosun University Vol.29 No.1

Background : This study aims to assess the efficacy of VATS pleurodesis in the treatment of spontaneous pneumothroax. Method and material : 131 consecutive patients who underwent videoUassisted thoracoscopy (VATS) bullectomy and pleurodcsis procedure in Chosun Uniservity Hospital between May 1999 and June 2003 were included in the study. Theses patients were performed the VATS with Talc pleurodesis (Group A, n=72), VATS with Doxycycline pleurodesis (Group B, n=36), and VATS with reinforcement by absorbable mesh (Surgicel_(R)) and fibrin glue (Group C, n=23). The follow-up period was from 4 months to 30 years. Result: The patient's population consisted of 117 men and 14 women, and the mean age of the patients was 25.6 years. Recurrence rate was 1.4% ( 1 of 72 patients) in group A, 5.6% (2 of 36 patients) in groug B and 0% (0 of 23 patients) in group C. The median removal period of drainage chest tube was 3.3 days. Conclusion : Careful surgical technique is fundamental in reducing early air leakage and recurrence. Moreover, the covering of the suture area with an absorbable mesh and the fibrin glue is safe and effective method with an acceptable recurrence rate.