Erythropoietin priming improves the vasculogenic potential of G-CSF mobilized human peripheral blood mononuclear cells

Kang, Jeehoon,Yun, Ji-Yeon,Hur, Jin,Kang, Jin-A,Choi, Jae-Il,Ko, Seung Bum,Lee, Jaewon,Kim, Ju-Young,Hwang, In-Chang,Park, Young-Bae,Kim, Hyo-Soo Oxford University Press 2014 Cardiovascular research Vol.104 No.1

<P><B>Aims</B></P><P>From our previous clinical trials, intracoronary infusion of granulocyte-colony stimulating factor (G-CSF)-mobilized peripheral blood mononuclear cells (<SUP>mob</SUP>PBMCs) proved to be effective in improving myocardial contractility and reducing infarct volume in acute myocardial infarction. We tested the effect of priming <SUP>mob</SUP>PBMCs with erythropoietin (EPO) to augment its therapeutic efficacy.</P><P><B>Methods and results</B></P><P><SUP>mob</SUP>PBMCs were obtained from healthy volunteers after a 3-day subcutaneous injection of G-CSF (10 μg/kg). About 40% of <SUP>mob</SUP>PBMCs were EPO receptor (EPOR) (+) and responded to 6 h EPO-priming (10 IU/mL) by increasing the expression of vasculogenic factors (i.e. IL8, IL10, bFGF, PDGF, MMP9) and adhesion molecules (i.e. integrin αV, β1, β2, β8) through the JAK2 and Akt pathway. These responses were also observed in PBMCs from elderly patients with coronary disease. The conditioned media from EPO-primed <SUP>mob</SUP>PBMCs contained various cytokines such as IL8, IL10, TNFα, and PDGF, which enhanced the migration and tube formation capability of endothelial cells. EPO-primed <SUP>mob</SUP>PBMCs also showed increased adhesion on endothelial cells or fibronectin. Augmented vasculogenic potential of EPO-primed <SUP>mob</SUP>PBMCs was confirmed in a Matrigel plug assay, ischaemic hindlimb, and myocardial infarction models of athymic nude mice. There were two action mechanisms: (i) cellular effects confirmed by direct incorporation of human <SUP>mob</SUP>PBSCs into mouse vasculature and (ii) indirect humoral effects confirmed by the therapeutic effect of the supernatant of EPO-primed <SUP>mob</SUP>PBMCs.</P><P><B>Conclusion</B></P><P>Brief <I>ex vivo</I> EPO-priming is a novel method to augment the vasculogenic potential of human <SUP>mob</SUP>PBMCs, which would help to achieve better results after intracoronary infusion in myocardial infarction patients.</P>

산삼 배양액 급여가 돈육의 이화학적 특성에 미치는 영향

진상근,김일석,송영민,허선진,박기훈,정기종,김동훈,노정만 진주산업대학교 산업과학기술연구소 2006 산업과학기술연구소보 Vol.- No.13

Berkshire를 이용하여 70일간 산삼 배양액 급여 시 육의 이화학적 특성에 미치는 영향은 다음과 같다. 산삼 배양액의 급여에 의해 육의 수분, 조단백, 조지방, 회분, pH, 가열감량, 콜레스테롤 함량, 육색, 조직감의 응집성과 검성, 포화지방산 및 불포화지방산의 비율은 차이를 보이지 않았다. 보수력, 지방색 L^(*)값, 지방산 중 linoleic acid(18:2), arachidonic acid(20:4), 필수지방산의 함량, 아미노산 중 threonine, glutamic acid, valine, isoleucine, leucine, histidine, lysine 및 총아미노산 함량은 산삼 배양액의 급여에 의해 감소하였다. 전단가, 조직감의 표면경도, 경도, 씹힘성, 지방산 중 stearic acid(18:0), oleic acid(18:1) 함량, 아미노산 중 serine, glycine, cystine, methionine, tyrosine 및 phenylalanine의 함량은 산삼 배양액의 급여에 의해 증가하였다. 본 연구결과를 종합하면 산삼 배양액의 급여는 돈육의 경도를 증가시키는 효과 외에 뚜렷한 이화학적인 차이를 나타내지 않았다. 이러한 이유는 급여된 산삼 배양액 내에 함유된 생리활성 물질의 함량이 낮고, 급여수준이 낮은 결과로 사료되어진다. A total of 60 pigs (Berkshire) were used to investigate the effect of dietary artificial culture medium of wild ginsengs (CMG) on physico-chemical characteristics of pork. About 60 kg pigs were randomly alloted into one of two experimental diet groups (C:commercial diet feed; T:commercial diet + 1 L CMG per day for 70days). Pigs were slaughtered at approximately 110 kg live weight, and chemical composition and physico-chemical characteristics were measured in pork loin. Moisture, crude protein, crude fat and ash were not significantly different by dietary artificial culture medium of wild ginsengs. Water-holding capacity was decreased by dietary artificial culture medium of wild ginsengs, while shear force was increased. However, pH, cooking loss and cholesterol contents were not significantly different between two different treatments. In color, L^(*) of fat was decreased by dietary artificial culture medium of wild ginsengs. Brittleness, hardness and chewiness were increased in dietary artificial culture medium of wild ginsengs group. In fatty acid, stearic acid (18:0) and oleic acid (18:1) were higher in dietary artificial culture medium of wild ginsengs group, whereas linoleic acid (18:2) and arachidonic acid (20:4) were higher in control group. In amino acid, serine, glycine, cystine, methionine, tyrosine and phenylalanine were higher in dietary artificial culture medium of wild ginsengs group, whereas threonine, glutamic acid, valine, isoleucine, leucine, histidine and lysine were higher in control group.

국내에서 파상풍의 연령별 면역혈청학적 역학연구

강진한,허재균,김종현,이경일,박수은,마상혁,이명숙,반상자,홍성화,조대현,이성호 대한감염학회 2001 감염 Vol.33 No.2

Background : The incidence of tetanus in adults and neonatal tetanus have been markedly reduced by world-wide use of DTP vaccines. But, tetanus is still one kind of major health problems in many developing countries, and several serosurvey stduies in developed ountries revealed that substantial proportions of adult opulation may lack immunity against tetanus and imunity level against tetanus is continuously decreasing by age. In Korea, tetanus outbreaks have been disappeared since the 1980s by high acceptant DTaP vaccination rates. Annually, few tetanus patient has been reported since 1990s. But, there knave been no seroepide-miological studies to tetanus, no trials to assess tetanuts immunizations. And we do not use Td vaccine in adults for maintaning tetanus immunity. In this aspect, we conducted age related survey of immunity to tetanus and indirectly assessed the immunogenecity of tetanus vaccines, used in Korea. Methods : For the evaluation of age related sero-survey of tetanus immunity in Korean population, study subjects were classified into 16 groups (A∼J group; below 10 years with one year interval, K∼O group; 11~60 yrs with 10 years interval, p group; over 60 yrs). The numbers of each group were 100, and sex distributions of each group were almostly equal. And far the indirect assessment of tetanus immunization in Korean children, children under 15 years old age were classified into 6 groups (I∼VI) according to the status of DTaP vaccination. The numbers of this each group were 50, and sex ratio was almostly equal. Specific IgG antibody to tetanus toxin were detected by ELISA. And the ANOVA repeated t-test was used to compare antibody levels in study groups. Results : In age related groups, the antibody levels to tetanus toxin were well maintained until 20 years old age group (L group), but thereafter the titers abruptly decreased below 0.1 IU/mL and over 75% populations among the groups over 30 years old age needed maintenance of protective immunity to tetanus. The antibody level of male was statistically higher than that of female in P group. In the groups related DTaP vaccination status, the antibody titer was very low in pre-vaccination group (I), but the titers after primary vaccinations were sharply increased and highly maintained until 15 years. Conclusion : The results of our study revealed that the immunity to tetanus was dramatically decreased in age groups over 30 years old. This result indicates that Td vaccination program in adults should be considered for maintenance of immunity to tetanus. And our study indicate that DtaP vaccination programs and vaccines, used in Korea, are effective for acquisition and maintenance of tetanus immunity in Korean children. (Korean J Infect 33:104~111, 2001)

Involvement of E-selectin in recruitment of endothelial progenitor cells and angiogenesis in ischemic muscle

Oh, Il-Young,Yoon, Chang-Hwan,Hur, Jin,Kim, Ji-Hyun,Kim, Tae-Youn,Lee, Choon-Soo,Park, Kyung-Woo,Chae, In-Ho,Oh, Byung-Hee,Park, Young-Bae,Kim, Hyo-Soo American Society of Hematology 2007 Blood Vol.110 No.12

<B>Abstract</B><P>E-selectin plays critical roles in tethering leukocytes to endothelial cells (ECs). We studied the role of E-selectin in endothelial progenitor cell (EPC) homing and vasculogenesis. After ischemia, the expression of E-selectin on ECs peaked 6 to 12 hours and returned to baseline at 24 hours, whereas the level of soluble E-selectin (sE-selectin) in serum increased over 24 hours and remained high at day 7. Mouse bone marrow-derived EPCs expressed not only E-selectin but also its ligand. Homing of circulating EPCs to ischemic limb was significantly impaired in E-selectin knock-out mice, as well as wild-type mice pretreated with blocking antibody against E-selectin, which was rescued by local sE-selectin injection. Mechanism for this is that sE-selectin stimulated not only ECs to express ICAM-1, but also EPCs to secrete interleukin-8 (IL-8), leading to enhanced migration and incorporation to ECs capillary formation. In therapeutic aspect, local treatment with sE-selectin enhanced efficacy of EPC transplantation for vasculogenesis and salvage of ischemic limb. Conversely, when E-selectin was knocked down by E-selectin small interfering RNA, blood flow recovery after EPC transplantation was significantly impaired. But this impaired vasculogenesis was rescued by sE-selectin. In conclusion, these data demonstrate E-selectin is a pivotal molecule for EPCs' homing to ischemic limb and vasculogenesis.</P>

Staphylococcus aureus와 Coagulase-Negative Staphylococcus Species에 대한 Arbekacin의 시험관내 항균력

위성헌,강진한,허동호,이동건,김상일,김양리,최정현,김종현,유진흥,허재균,신완식,강문원 대한감염학회 2001 감염 Vol.33 No.4

Background : Most strains of methicillin-resistant Staphylococcus aureus (MRSA) now exhibit high-level resistance to various antibiotics, such as β -lactam antibiotics, aminoglycosides, macrolides, tetracyclines and quinolones. Recent reports describing the therapeutic failure of vancomycin for MRSA infections have arisen considerable concerns regarding the emergence of MRSA strains, which will require new therapeutic agents. Arbekacin, an aminoglycoside antibiotic, has antibacterial activity against both gram-positive and gram-negative bacteria and is stable in the presence of aminoglycoside inactivating enzymes produced by S. aureus. In this study, we compared the antibacterial activity of arbekacin with those of vancomycin, gentamicin, and amikacin against Staphylococcus aureus (S. aureus) and coagulase-negative staphylococci (CNS). Methods : For a collection of 549 S. aureus and 251 CNS isolates from three Catholic University Hospitals in Korea, minimum inhibitory concentrations (MICs) of arbekacin, vancomycin, amikacin and gentamicin were determined by agar dilution method using Mueller-Hinton agar according to NCCLS (National Committee for Clinical Laboratory Standards, USA)criteria. Results : Among 549 S. aureus isolates, 278 isolates were MRSA and 271 isolates were methicil sensitive S. aureus (MSSA). MIC50 & MIC90 of arbekacin against 549 S. aureus were 0.5 & 1 ㎍/mL, and MIC50 & MIC90 of vancomycin were 1 & 1 ㎍/mL. MIC of arbekacin against 549 S. aureus isolates ranges from 0.03 to 4 ㎍/mL, and MIC of vancomycin against 549 S. aureus ranges from 0.25 to 2 ㎍/mL. MIC90 of amikacin against 549 S. aureus was 32㎍/mL, and that of gentamicin was 128 ㎍/mL. MICs of amikacin and gentamicin were variable, ranging from 0.125 to 256, and otherwise arbekacin and vancomycin revealed relatively narrow range of MICs. MIC90 of arbekacin against 278 MRSA isolates & 271 MSSA were 1 & 0.5 ㎍/mL, and those of vancomycin against MRSA & MSSA were 1 & 1 ㎍/mL. MIC90 of amikacin against 278 MRSA & 271 MSSA isolates were 32 & 4 ㎍/mL, and that of gentamicin against MRSA & MSSA isolates were 128 & 32 ㎍/mL respectively. Among 251 CNS isolates, 122 isolates were MRCNS and 129 were MSCNS. MICSO & MIC90 of arbekacin against 251 CNS isolates were 0.25 & 2 ㎍/mL, and those of vancomycin were 1 & 2 ㎍/mL. MIC of arbekacin against 251 CNS isolates ranges from 0.015 to 32 ㎍/mL, and that of vancomycin isolates ranges from 0.25 to 2 ㎍/mL, MIC90 of arbekacin against 122 MRCNS & 129 MSCNS isolates were 2&0.3 ㎍/ML, and those of vancomycin were 2&s ㎍/ML. MIC90 of amikacin against 251 CNS isolates was 32 ㎍/ML, and that of gentamicin was 128 ㎍/ML for CNS. MIC90 of amikacin against 122 MRCNS & 129 MSCNS isolates were 128 & 8㎍/mL, and those of gentamicin ere 256 & 32 ㎍/mL. Conclusion : Considering above results, arbekacin can be useful agent against most strains of MRSA and MRCMS, which exhibit high-level resistance to amikacin and gentamicin. (Korea J Infect Dis 33:254~260, 2001)

자연패사(自然貝砂)의 특성과 토양산도 교정력(矯正力)에 관한 연구 : 제주도 및 진도산을 중심으로

박영대,이윤환,한기학,김복진,허일봉 한국농화학회 1972 Applied Biological Chemistry (Appl Biol Chem) Vol.15 No.3

The characteristic and efficiency of natural shell sand in the coast of Cheju-Do and Jin-Do were studied to apply as agricultural lime for the soil acid adjustment. 1. The alkalinity of shell sand from Cheju coast was higher than that of Jindo and more weathered into fine particles. The particle size distributions of Cheju shell sand were condensed finer particles than 32 mesh in Cheju shell sands and in more coarser particles than 32 mesh in Jindo one. 2. The effect of Cheju shell sand on increasing soil pH value in the upland condition was low at the beginning but more gradually increased after 8 weeks from the treatment than ground lime, and Jindo was very dull during the period of treatment. The commercial lime crushed from Jindo was approximately equal to the ground lime. 3. In the submerged condition, the shell sand of Cheju reacted with soil acid more quickly than ground lime after 2 days, and Jindo was very slow. 4. The relative efficiency of various particle size fraction of the shell sand was superior to the ground lime. The lime particles between 9 to 14 mesh and 20 mesh had more remarkable difference of pH value than other fine particles. The efficiency among finer sizes than 20 mesh particle was approximately equal to each others. 5. The shell sand from the Cheju would be applied directly as agricultural lime without any treatment, and Jindo also can be expected to be effective as agricultural lime with crushing procedure.

Extractability and Degradation of Chicken Myofibrillar Protein during the Postmortem Storage

Il-Suk Kim,Sang-Keun Jin,Joong-Seok Min,Dea-Keun Shin,Sun-Jin Hur,Kyung-Hee Hah,Mooha Lee 한국국제농업개발학회 2005 韓國國際農業開發學會誌 Vol.17 No.1

Antispasmodic Effects of Junsibaekchul-San In Vivo and In vitro

Hur, Jin-Il,Byun, Joon-Seok,Kim, Dae-Jun The Physiological Society of Korean Medicine and T 2010 동의생리병리학회지 Vol.24 No.1

In Vivo and In vitro antispasmodic effects of Jun-Si-Baek-Chul-San, a Traditional Korean Polyherbal Medicineconsisted of 7 types of herbs were observed in the present study. To clarify the effects of Jun-Si-Baek-Chul-San, on accelerating small intestinal movement induced by the stimulation of cholinergic neurotransmission, we evaluated the effects of Jun-Si-Baek-Chul-San on In vivo carbachol (an acetylcholinergic agent)-accelerated mice small intestinal transit and on In vitro contractions induced by low-frequency electrostimulation, KCl, histamine or acetylcholine using isolated guinea pig ileum. To induce the acceleration of mice small intestinal transit, Carbachol 1 mg/kg was once subcutaneously dosed 15min before last administration of the test drugs. In the present study, Jun-Si-Baek-Chul-San 500, 250 and 125 mg/kg or domperidone 20 mg/kg were orally pretreated on the carbachol-accelerated mice small intestinal transit once a day for 7 days and the small intestinal transit rateof activated charcoal powder were monitored. In vitro assays, Jun-Si-Baek-Chul-San1, 0.1, 0.01 and 0.001 mg/ml or domperidone $2{\times}10^{-5}M$ were treated 10min before ileal contraction was induced by filed stimulation, acetylcholine, KCl and histamine, and the % changes of contractions were observed compared to the treatment of inducer alone. In spontaneous contraction, the % changes of contractions were observed compared to treatment of vehicle alone at 10min after Jun-Si-Baek-Chul-San or domperidone treatment. The efficacy of Jun-Si-Baek-Chul-San was compared to those of domperidone. High concentration, 1 mg/ml of Jun-Si-Baek-Chul-San was found to decrease the spontaneous contraction of the isolated guinea-pig ileum. In addition, Jun-Si-Baek-Chul-San decrease contractions induced by electrostimulation, acetylcholine, histamine and KCl in the isolated guinea-pig ileum. In addition, Jun-Si-Baek-Chul-San effectively inhibited the accelerated small intestinal movement induced by carbachol stimulation of cholinergic neurotransmission in In vivo. Based on the results, although the exact molecular or action mechanism and which herbs or compound in Jun-Si-Baek-Chul-San are responsible for actions, it was concluded that Jun-Si-Baek-Chul-San normalization in the accelerated intestinal motility might be interfere with a variety of muscarinic, adrenergic and histaminic receptor activities or with the mobilization of calcium ions required for smooth muscle contraction non-specificly. Therefore, it is expected that Jun-Si-Baek-Chul-San will be promising as a prescription of clinical treatment of digestive tract disorders such as accelerated the motility of intestine, diarrhea or intestinal painful contractions.

Effect of extra virgin olive oil substitution for fat on quality of pork patty

Hur, Sun Jin,Jin, Sang Keun,Kim, Il Suk John Wiley Sons, Ltd. 2008 Journal of the science of food and agriculture Vol.88 No.7

<P>BACKGROUND:</P><P>Extra virgin olive oil (EVOO) is well known for its beneficial effects on human health. Thus, the purpose of this study was to investigate the effect of EVOO substitution for backfat on qualities of pork patty to avoid high consumption of animal-originated fat, because it is closely related to development of cardiovascular disease and obesity.</P><P>RESULT:</P><P>Water-holding capacity was higher in control (lean pork + 10% backfat) and T3 (lean pork + 5% backfat + 5% EVOO + 0.5% isolated soy protein + 0.5% carrageenan + 0.5% maltodextrin) than T1 (lean pork + 5% backfat + 5% EVOO + 0.5% isolated soy protein) and T2 (lean pork + 5% backfat + 5% EVOO + 0.5% isolated soy protein + 0.5% carrageenan). Hardness was higher in EVOO substitution for backfat patty samples than control. In sensory evaluation, the control was significantly higher in overall acceptability compared with EVOO substitution for backfat pork patty samples.</P><P>CONCLUSION:</P><P>The physical properties of pork patty made by EVOO substitution for backfat were stable as a commercial pork patty (control). However, sensory evaluation scores were higher in control compared to EVOO-substituted pork patty samples. Copyright © 2008 Society of Chemical Industry</P>

Antispasmodic Effects of Junsibaekchul-San In Vivo and In vitro

Jin Il Hur,Joon Seok Byun,Dae Jun Kim 한의병리학회 2010 동의생리병리학회지 Vol.24 No.1