金銀花 및 魚腥草가 人體 癌細胞柱에 미치는 影響

陳千植,崔政和,鄭鉉雨 대한동의병리학회 1996 동의생리병리학회지 Vol.10 No.1

국내 사망률 가장 높은 비율을 차지하고 있는 惡性腫瘍에 대하여 국내외적으로 다양하게 연구되고 있다. 그러나 아직까지도 腫瘍을 치료하기 위하여 手術治療·放射線治療·免疫治療·化學治療등의 많은 치료법을 개발하고, 또한 실험실 및 임상에서 많은 연구보고가 이루어지고 있지만 腫瘍의 치료원칙 및 치료약물에 대해서 미흡한 것이 국내의 현실이다. 일반적으로 抗癌劑를 이용한 化學治療등이 사용되고 있지만 이에 따른 副作用이 많아 본 연구자들은 抗癌劑와 韓藥材를 倂用投與하여 副作用을 최소화하고, 이에 따라 韓藥材가 正常細胞에 어떠한 영향을 미치고 , 癌腫細胞에는 어떠한 영향을 미치는가에 대하여 관찰한 결과 有意性이 있어 보고하게 되었다. 淸熱作用이 金銀花와 魚腥草를 인체의 皮膚癌細胞인 A431 細胞, 子宮癌細胞인 HeLa 細胞, 急性白血病細胞인 MOLT-4 細胞, 慢性骨髓性白血病細胞인 K562 細胞에 대한 細胞毒性과 抗癌劑인 mitomycin C와 倂用처리결과를 MTT assay를 통하여 관찰하였다. 또한 正常細胞에 대한 細胞毒性을 검색하기 위하여 마우스 섬유아세포(Balb/c3T3), 마우스 胸線 및 腥臟細胞, human lymphocyte에 미치는 細胞毒性을 검토하였다. 魚腥草는 慢性骨髓性白血病細胞柱에 직접적인 細胞毒性을 나타냈고, mitomycin C와 倂用 處理時 細胞毒性을 증가시켰으며, 마우스 섬유아細胞인 Balb/c 3T3 細胞의 增殖을 억제시켰다. 또한 金銀花는 마우스 胸線細胞의 增殖을 촉진시켰고, human lymphocyte의 增殖을 촉진시켰으며, 金銀花 및 魚腥草 모두는 마우스 腥臟細胞의 增殖을 촉진시켰다. The purpose of this research was to investigate effect of water extract of Flos Lonicerae and Herba Houttuyniae on the proliferation of human cancer cell-lines. The effects of Flos Lonicerae and Herba Houttyniae on the proliferation of A431, HeLa, MOLT-4, K562 cells, Balb/c 3T3 cells, mouse thymocytes, splenocytes and human lymphocytes were estimated by MTT colorimetric assay. 1. Flos Lonicerae and Herba Houttuyniae inhibited the proliferation of K562 cells. 2. The cytotoxicity of mitomycin C on K562 cells was decreased by the combination of Herba Houttuyniae. 3. Herba Houttuyniae inhibited the proliferation of Balb/c 3T3 cells. 4. Flos Lonicerae stimulated the proliferation of thymocytes. 5. Flos Lonicerae and Herba Houttuyniae stimulated the proliferation of splenocytes. 6. Flos Lonicerae stimulated the proliferation of human lymphocytes.

Ethyoene Oxide 폭로 근로자의 말초 임파구 자매염색분체 교환에 관한 연구

황천현,박종태,장성훈,김대성,이원진,김청식,김영환 大韓産業醫學會 1998 대한직업환경의학회지 Vol.10 No.4

Operators of hospital sterilizers who use ethylene oxide(EtO) were studied to determine the exposure of EtO level and the frequency of sister chromatid exchanges(SCEs) from June 12 to July 20, 1997. To evaluate SCEs in the peripheral blood cells, we selected 22 workers at the central supply room of 4 university hospitals and 22 unexposed workers at the same hospitals according to match sex, age, and smoking habit and also did questionnaires. The mean air concentrations(8-hr TWA) of EtO at 4 university hospitals were less than 1 ppm. The SCE frequencies in exposed workers to EtO and controls were normally distributed. The SCE frequencies in exposed workers to EtO and controls were 6.42±0.63, 5.86±0.69, respectively and their differences were statistically significant(p=0.0093). But there were no statistically significant differences in smoking, alcohol intake, coffee drinking. Especially smokers who exposed to EtO were increased SCE statistically significant than the exposed group who did not smoke.

免疫細胞 및 腫瘍細胞에 미치는 丹蔘의 效果

鄭鉉雨,姜聲度,陳千植 대한동의병리학회 1998 동의생리병리학회지 Vol.12 No.2

免疫은 人體內에서 어떤 요인으로 인해서든지 異物이 침입하거나 變異細胞가 발생하면 Immune system이 관여하여 異物은 물론 새로이 발생된 變異細胞를 非自己로 인식하여 처리하는 能力을 발휘함으로써 個體의 恒常性을 維持하려는 현상을 말하고, 이러한 免疫作用에 관여하는 細胞는 特異性 免疫에 관여하는 免疫細胞外에도 Macrophage, NK cell, 수지상세포 및 랑게르한스 세포등을 들 수 있으며, 腫瘍은 組織이 自律的으로 過剩 成長한 것으로 이것은 개체에 대해서 意義가 없거나 이롭지 않을뿐더러 正常組織에 대해서 破壞的인 작용을 하는 것으로 東醫學에서는 正氣補養 및 補血을 爲主로하면서 破積·活血·解鬱·行氣등의 治法들을 兼用하고 있고, 西醫學에서는 手術療法, 放射線療法, 化學療法 및 免疫療法과 遺傳子療法등을 使用하나 癌腫에 따른 感受性과 治療 後의 經過 그리고 副作用이 각기 다르고, 또 이에 따른 많은 문제점들을 안고 있는 것이다. 癌細胞의 增殖을 抑制하는 方法에는 necrosis와 apoptosis가 있는데, apoptosis란 necrosis(세포괴사)와는 대조되는 용어로 초기에 핵의 응축, 세포질의 응축, 수포상의 세포돌기 형성등이 관찰되고, 生體內의 apoptosis는 腫瘍發生時 macrophage에서 분비되는 각종 cytokine에 의해 일어나거나 cytotoxic T-lymphocyte에 의해 일어나는 것으로 抗癌劑들의 부작용 및 tumor regression과 관계가 있는 것으로 알려져 있다. Nitric oxide(NO)는 cNOS와 iNOS 2 종류가 있으며 cNOS는 vascular endothelium 및 brain에서, iNOS는 활성화된 macrophage 및 여러 세포에서 발견되었다. Macrophage가 생산하는 NO는 生體內에서 癌細胞를 공격하여 傷害시키는 癌 免疫의 effector로서 그 역할을 하고 있고, 癌細胞의 增殖을 抑制한다. 丹蔘은 活血祛瘀시키면서 凉血安神시기 때문에 産後瘀滯腹痛·경폐징하·心腹刺痛과 熱毒으로 인한 癰腫·瘡瘍등에 利用되고, 最近에는 肝臟腫大와 動脈硬化症, 그리고 食道癌·胸腺癌 및 腹腔腫瘍에도 사용하고 있는 藥物로 本 硏究에서는 丹蔘에 대한 癌細胞 增殖 抑制作用과 免疫細胞 增强作用을 관찰한 결과 丹蔘은 In vitro上에서 A431 cell line과 KHOS-NP cell line의 增殖을 抑制하지는 않았지만 마우스 胸腺 및 脾臟細胞의 增殖을 活性化시켰다. 그러나 腹腔 macrophage에서 생성되는 NO는 丹蔘에 의해 in vitro상에서 억제되었고 in vivo상에서도 對照群과 큰 차이를 보이지 않았는데, 이는 丹蔘이 腹腔 macrophage의 活性化보다는 T-lymphocytes나 splenocytes의 增殖에 작용하는 것으로 思料된다. To investigate effect of water extract of Salviae Radix on human cancer cell-lines and immunocytes, this research estimated the proliferation of A431 cell lines, KHOS-NP cell lines, mouse thymocytes and mouse splenocytes, nitric oxide(NO) production from peritoneal macrophages, apoptosis and sub-population of mouse thymocytes. The result were obtained as follows ; 1. Salviae Radix wasn't effect the proliferation of A431 cell lines and KHOS-NP cell lines. 2. Salviae Radix was accelerate the proliferation of mouse thymocytes and splenocytes. 3. Salviae Radix was not accelerate the NO production from peritoneal macrophages in vitro. 4. Salviae Radix was inhibited the NO production from peritoneal macrophages in vivo. 5. Salviae Radix was accelerate the induction of apoptosis and sub-population of the mouse thymocytes.

보리순의 영양성분 조성

서혜지,최용민,김상천,이준수,이기택,정명근,천지연,황진봉,남진식,서우덕,황영,김행란,김세나 忠北大學校 農業科學硏究所 2015 農業科學硏究 Vol.31 No.2

심정지환자에서 epinephrine투여시 혈중 catecholamine의 변화

임경수,황성오,이부수,이강현,이진웅,임종천,김영식,김선만,김 현 대한응급의학회 1995 대한응급의학회지 Vol.6 No.1

Background : Cardiac arrest is a potent stimulus for the release of endogenous catecholamine and high plasma catecholamine concentration has been reported during cardiopulmonary resuscitation(CPR). Few data were present about the clinical effect of high plasma catecholamine and endogenous catecholamine response to exogenous epinephrine administration during CPR. Purpose : This study was designed to evaluate endogenous catecholamine response to cardiac arrest and exogenously administered epinephrine during CPR. Method : In 21 cardiac arrest victims, blood sample for epinephrine and norepinephrine was drawn before and 1 minute after each epinephrine administration during CPR. 1mg of epinephrine was administered immediately after intravenous access and 3 minute interval during CPR. Plasma catecholamines were quantitated by high-performance liquid chromatography. Result : Baseline plasma epinephrine concentraqtion was higher in patients with cardiac arrest than normal controls (985±1627 vs 194±173 pg/ml, p<0.05). Baseline plasma norepinephrine concentration was also elevated in patients with cardiac arrest than normal controls, although statistically insignificnat(1965±4915 vs 360±250 pg/ml). Plasma epinephrine concentration in cardiac arrest patients was significantly elevated after exogenous administration of epinephrine, but its response was blunted after the third does of epinephrine. Plasma norepinephrine concentration in cardiac arrest patients was also elevated, but its response was observed only after the first epinephrine administration. Plasma epinephrine concentration was persistently higher in patients with return of spontaneous circulation (ROSC) than patients without ROSC. there was no significant difference of plasma norepinephrine concentration whether spontaneous circulation was restored or not. Endogenous epinephrine response to exogenous epinephrine was not observed in patients having prolonged arrest time over 20 minutes. Conclusion : Results of this study suggests that significant amount of catecholamine is released by exogenous epinephrine administration in spite of high plasma catecholamine concentration, and degree of endogenous catecholamine response influences resuscitation outcome.

직장암의 자율신경보존 근치술에서 측방골반림프절절제의 적용

김진천,유창식,박건춘,김진천,유창식,박건춘 대한대장항문학회 1994 Annals of Coloproctolgy Vol.10 No.3

Materials Testing Techniques : INVESTIGATION OF INITIAL DEFORMATION BEHAVIOR DURING SMALL RUNCH TEST

Jin Sik Cheon,In Sup Kim 대한금속재료학회 ( 구 대한금속학회 ) 1995 Advanced Material and Processing Vol.2 No.-

Proteomic Assessment of the Relevant Factors Affecting Pork Meat Quality Associated with Longissimus dorsi Muscles in Duroc Pigs

Cho, Jin Hyoung,Lee, Ra Ham,Jeon, Young-Joo,Park, Seon-Min,Shin, Jae-Cheon,Kim, Seok-Ho,Jeong, Jin Young,Kang, Hyun-sung,Choi, Nag-Jin,Seo, Kang Seok,Cho, Young Sik,Kim, MinSeok S.,Ko, Sungho,Seo, Jae Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.11

Meat quality is a complex trait influenced by many factors, including genetics, nutrition, feeding environment, animal handling, and their interactions. To elucidate relevant factors affecting pork quality associated with oxidative stress and muscle development, we analyzed protein expression in high quality longissimus dorsi muscles (HQLD) and low quality longissimus dorsi muscles (LQLD) from Duroc pigs by liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based proteomic analysis. Between HQLD (n = 20) and LQLD (n = 20) Duroc pigs, 24 differentially expressed proteins were identified by LC-MS/MS. A total of 10 and 14 proteins were highly expressed in HQLD and LQLD, respectively. The 24 proteins have putative functions in the following seven categories: catalytic activity (31%), ATPase activity (19%), oxidoreductase activity (13%), cytoskeletal protein binding (13%), actin binding (12%), calcium ion binding (6%), and structural constituent of muscle (6%). Silver-stained image analysis revealed significant differential expression of lactate dehydrogenase A (LDHA) between HQLD and LQLD Duroc pigs. LDHA was subjected to in vitro study of myogenesis under oxidative stress conditions and LDH activity assay to verification its role in oxidative stress. No significant difference of mRNA expression level of LDHA was found between normal and oxidative stress condition. However, LDH activity was significantly higher under oxidative stress condition than at normal condition using in vitro model of myogenesis. The highly expressed LDHA was positively correlated with LQLD. Moreover, LDHA activity increased by oxidative stress was reduced by antioxidant resveratrol. This paper emphasizes the importance of differential expression patterns of proteins and their interaction for the development of meat quality traits. Our proteome data provides valuable information on important factors which might aid in the regulation of muscle development and the improvement of meat quality in longissimus dorsi muscles of Duroc pigs under oxidative stress conditions.

Regional Differences of Proteins Expressing in Adipose Depots Isolated from Cows, Steers and Bulls as Identified by a Proteomic Approach

Cho, Jin Hyoung,Jeong, Jin Young,Lee, Ra Ham,Park, Mi Na,Kim, Seok-Ho,Park, Seon-Min,Shin, Jae-Cheon,Jeon, Young-Joo,Shim, Jung-Hyun,Choi, Nag-Jin,Seo, Kang Seok,Cho, Young Sik,Kim, MinSeok S.,Ko, Sun Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.8

Adipose tissue in the loin muscle area of beef cattle as a marbling factor is directly associated with beef quality. To elucidate whether properties of proteins involved in depot specific adipose tissue were sex-dependent, we analyzed protein expression of intramuscular adipose tissue (IMAT) and omental adipose tissue (OMAT) from Hanwoo cows, steers, and bulls of Korean native beef cattle by liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based proteomic analysis, quantitative polymerase chain reaction (PCR) and western blot analysis. Two different adipose depots (i.e. intramuscular and omental) were collected from cows (n = 7), steers (n = 7), or bulls (n = 7). LC-MS/MS revealed a total of 55 and 35 proteins in IMAT and OMAT, respectively. Of the 55 proteins identified, 44, 40, and 42 proteins were confirmed to be differentially expressed in IMAT of cows, steers, and bulls, respectively. In OMAT of cows, steers, and bulls, 33, 33, and 22 were confirmed to be differentially expressed, respectively. Tropomyosin (TPM) 1, TPM 2, and TPM3 were subjected to verification by quantitative PCR and western blot analysis in IMAT and OMAT of Hanwoo cows, steers, and bulls as key factors closely associated with muscle development. Both mRNA levels and protein levels of TPM1, TPM2, and TPM3 in IMAT were lower in bulls compared to in cows or steers suggesting that they were positively correlated with marbling score and quality grade. Our results may aid the regulation of marbling development and improvement of meat quality grades in beef cattle.

직장암수술시 자동봉합기 사용의 경험

김진천(Jin Cheon Kim),정희원(Hee Won Chung),유창식(Chang Sik Yu),이한일(Han Il Lee),박상규(Sang Kyu Park),한명식(Myung Sik Han),박건춘(Kun Choon Park) 대한외과학회 1997 Annals of Surgical Treatment and Research Vol.53 No.3