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Jimyeong Ha,Jeeyeon Lee,Hyemin Oh,Hyun Jung Kim,Yukyung Choi,Yewon Lee,Yujin Kim,Heeyoung Lee,Sejeong Kim,Yohan Yoon 한국축산식품학회 2020 한국축산식품학회지 Vol.40 No.6
A dynamic model was developed to predict the Escherichia coli cell counts in pig trotters at changing temperatures. Five-strain mixture of pathogenic E. coli at 4 Log CFU/g were inoculated to cooked pig trotter samples. The samples were stored at 10℃, 20℃, and 25℃. The cell count data was analyzed with the Baranyi model to compute the maximum specific growth rate (μmax) (Log CFU/g/h) and lag phase duration (LPD) (h). The kinetic parameters were analyzed using a polynomial equation, and a dynamic model was developed using the kinetic models. The model performance was evaluated using the accuracy factor (Af), bias factor (Bf), and root mean square error (RMSE). E. coli cell counts increased (p<0.05) in pig trotter samples at all storage temperatures (10℃-25℃). LPD decreased (p<0.05) and μmax increased (p<0.05) as storage temperature increased. In addition, the value of h0 was similar at 10℃ and 20℃, implying that the physiological state was similar between 10℃ and 20℃. The secondary models used were appropriate to evaluate the effect of storage temperature on LPD and μmax. The developed kinetic models showed good performance with RMSE of 0.618, Bf of 1.02, and Af of 1.08. Also, performance of the dynamic model was appropriate. Thus, the developed dynamic model in this study can be applied to describe the kinetic behavior of E. coli in cooked pig trotters during storage.
( Jimyeong Ha ),( Hyemin Oh ),( Sejeong Kim ),( Jeeyeon Lee ),( Soomin Lee ),( Heeyoung Lee ),( Yukyung Choi ),( Sung Sil Moon ),( Kyoung-hee Choi ),( Yohan Yoon ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.1
Listeria monocytogenes can asymptomatically inhabit the human intestine as a commensal bacterium. However, the mechanism by which L. monocytogenes is able to inhabit the intestine without pathogenic symptoms remains unclear. We compared the invasion efficiency of L. monocytogenes strains with the 268- and 385-bp-long actA gene. Clinical strains SMFM-CI-3 and SMFM-CI-6 with 268-bp actA isolated from patients with listeriosis, and strains SMFM-SI-1 and SMFM-SI-2 with the 385-bp gene isolated from carcasses, were used for inoculum preparation. The invasion efficiency of these strains was evaluated using Caco-2 cells (intestinal epithelial cell line), prepared as normal and healthy cells with tightened tight junctions and senescent cells with loose tight junctions that were loosened by adriamycin treatment. The invasion efficiency of L. monocytogenes strains with the 268-bp-long actA gene was 1.1-2.6-times lower than that of the strains with the 385-bp-long gene in normal and healthy cells. However, the invasion efficiency of both types of strains did not differ in senescent cells. Thus, L. monocytogenes strains with the 268-bp-long actA gene can inhabit the intestine asymptomatically as a commensal bacterium, but they may invade the intestinal epithelial cells and cause listeriosis in senescent cells.
Mathematical Models to Describe the Kinetic Behavior of Staphylococcus aureus in Jerky
Jimyeong Ha,Jeeyeon Lee,Soomin Lee,Sejeong Kim,Yukyung Choi,Hyemin Oh,Yujin Kim,Yewon Lee,Yeongeun Seo,Yohan Yoon 한국축산식품학회 2019 한국축산식품학회지 Vol.39 No.3
The objective of this study was to develop mathematical models for describing the kinetic behavior of Staphylococcus aureus (S. aureus) in seasoned beef jerky. Seasoned beef jerky was cut into 10-g pieces. Next, 0.1 mL of S. aureus ATCC13565 was inoculated into the samples to obtain 3 Log CFU/g, and the samples were stored aerobically at 10°C, 20°C, 25°C, 30°C, and 35°C for 600 h. S. aureus cell counts were enumerated on Baird Parker agar during storage. To develop a primary model, the Weibull model was fitted to the cell count data to calculate Delta (required time for the first decimal reduction) and ρ (shape of curves). For secondary modeling, a polynomial model was fitted to the Delta values as a function of storage temperature. To evaluate the accuracy of the model prediction, the root mean square error (RMSE) was calculated by comparing the predicted data with the observed data. The surviving S. aureus cell counts were decreased at all storage temperatures. The Delta values were longer at 10°C, 20°C, and 25°C than at 30°C and 35°C. The secondary model well-described the temperature effect on Delta with an R2 value of 0.920. In validation analysis, RMSE values of 0.325 suggested that the model performance was appropriate. S. aureus in beef jerky survives for a long period at low storage temperatures and that the model developed in this study is useful for describing the kinetic behavior of S. aureus in seasoned beef jerky.
Kinetic Behavior of Salmonella on Low NaNO2 Sausages during Aerobic and Vacuum Storage
Jimyeong Ha,Eunji Gwak,Mi Hwa Oh,Beomyoung Park,Jeeyeon Lee,Sejeong Kim,Heeyoung Lee,Soomin Lee,Yohan Yoon 한국축산식품학회 2016 한국축산식품학회지 Vol.36 No.2
Abstract This study evaluated the growth kinetics of Salmonella spp. in processed meat products formulated with low sodium nitrite (NaNO2). A 5-strain mixture of Salmonella spp. was inoculated on 25-g samples of sausages formulated with sodium chloride (NaCl) (1.0%, 1.25%, and 1.5%) and NaNO2 (0 and 10 ppm) followed by aerobic or vacuum storage at 10°C and 15°C for up to 816 h or 408 h, respectively. The bacterial cell counts were enumerated on xylose lysine deoxycholate agar, and the modified Gompertz model was fitted to the Salmonella cell counts to calculate the kinetic parameters as a function of NaCl concentration on the growth rate (GR; Log CFU/g/h) and lag phase duration (LPD; h). A linear equation was then fitted to the parameters to evaluate the effect of NaCl concentration on the kinetic parameters. The GR values of Salmonella on sausages were higher (p<0.05) with 10 ppm NaNO2 concentration than with 0 ppm NaNO2. The GR values of Salmonella decreased (p<0.05) as NaCl concentration increased, especially at 10oC. This result indicates that 10 ppm NaNO2 may increase Salmonella growth at low NaCl concentrations, and that NaCl plays an important role in inhibiting Salmonella growth in sausages with low NaNO2.
Jimyeong Ha,Sejeong Kim,Jeeyeon Lee,Soomin Lee,Heeyoung Lee,Yukyung Choi,Hyemin Oh,Yohan Yoon 한국축산식품학회 2017 한국축산식품학회지 Vol.37 No.3
The identification of pork in commercially processed meats is one of the most crucial issues in the food industry because of religious food ethics, medical purposes, and intentional adul-teration to decrease production cost. This study therefore aimed to develop a method for the detection of pork adulteration in meat products using primers specific for pig mitochondrial DNA. Mitochondrial DNA sequences for pig, cattle, chicken, and sheep were obtained from GenBank and aligned. The 294-bp mitochondrial DNA D-loop region was selected as the pig target DNA sequence and appropriate primers were designed using the MUSCLE program. To evaluate primer sensitivity, pork-beef-chicken mixtures were prepared as follows: i) 0% pork-50% beef-50% chicken, ii) 1% pork-49.5% beef-49.5% chicken, iii) 2% pork-49% beef- 49% chicken, iv) 5% pork-47.5% beef-47.5% chicken, v) 10% pork-45% beef-45% chicken, and vi) 100% pork-0% beef-0% chicken. In addition, a total of 35 commercially packaged products, including patties, nuggets, meatballs, and sausages containing processed chicken, beef, or a mixture of various meats, were purchased from commercial markets. The primers developed in our study were able to detect as little as 1% pork in the heat treated pork-beef-chicken mixtures. Of the 35 processed products, three samples were pork positive despite being labeled as beef or chicken only or as a beef-chicken mix. These results indicate that the developed primers could be used to detect pork adulteration in various processed meat prod-ucts for application in safeguarding religious food ethics, detecting allergens, and preventing food adulteration.
놀이중심 개정 누리과정에서 경험하는 유아교사의 갈등과 변화
홍지명 ( Jimyeong Hong ) 사단법인 아시아문화학술원 2020 인문사회 21 Vol.11 No.6
본 연구의 목적은 놀이중심 교육과정인 2019 개정 누리과정에서 경험하는 유아교사의 갈등과 변화를 살펴보는 데 있다. 이를 위해 개정 누리과정을 실행하기 위해 적극적으로 노력하고 있는 유아교사10명을 참여자로 선정하였으며, 참여교사들과 개별면담을 통해 자료를 수집하였다. 이를 분석한 연구결과는 다음과 같다. 첫째, 놀이중심 개정 누리과정에서 경험하는 유아교사의 갈등은 놀이중심교육에 대한 모호성과 부담감, 교사로서의 나의 역량에 대한 의심, 자율적 교육계획에 대한 어려움, 이상과 현실 간의 갈등으로 나타났다. 둘째, 놀이중심 개정 누리과정에서 경험하는 유아교사의 변화는 고정관념 허물기, 마음의 여유를 갖고 기다리기, 놀이 관찰자 및 지원자로서의 역할 변화, 놀이 연구자로서의 교사 능력을 키우기였다. 이상의 결과를 바탕으로 놀이중심 개정 누리과정의 안정적인 현장 정착과 실현을 위한 요건을 고찰하고 교사 지원 방안을 위한 기초자료로 활용될 수 있다는 점에 본 연구의 의의가 있다. The purpose of this study is to examine the conflicts and changes experienced by early childhood teachers in the 2019 revised Nuri curriculum, a play-oriented curriculum. To this end, 10 early childhood teachers who actively worked to implement the revised Nuri curriculum were selected as participants and data were collected through individual interviews with participating teachers. The results of the analysis are as follows. First, conflicts among early childhood teachers experienced in the Nuri curriculum for the revision of the play-centered system were revealed as ambiguity and burden on play-oriented education, doubts about my ability as a teacher, difficulties in the autonomous educational community, and conflicts between ideals and reality. Second, the changes that appeared to infant teachers in the Nuri curriculum for the revision of the play center were to break down stereotypes, to wait with composure, to change the role of play watchers and applicants, and to develop the ability of teachers as play researchers. Based on the above results, it is meaningful in that the requirements for stable site settlement and realization of the play-oriented revised Nuri curriculum can be considered and used as basic data for ways to support teacher capacity building.
극화놀이를 활용한 공감교육이 유아의 배려적 사고 및 창의적 인성에 미치는 영향
홍지명 ( Jimyeong Hong ) 사단법인 아시아문화학술원 2021 인문사회 21 Vol.12 No.1
본 연구는 극화놀이를 활용한 공감교육이 유아의 배려적 사고 및 창의적 인성에 미치는 영향을 살펴보는 데 있다. 연구대상은 M지역의 D유아교육기관 만 5세 유아 40명이다. 연구도구는 배려적 사고 척도, 창의적 인성 검사 도구를 사용하였다. 실험처치는 실험집단은 극화놀이를 통한 공감교육을, 비교집단은 이야기나누기를 통한 공감교육을 실시하였다. 자료분석은 실험처치의 효과를 검증하기 위하여 SPSS 24.0을 사용하여 t-검증을 통해 분석하였다. 연구결과, 첫째, 극화놀이를 활용한 공감교육은 유아의 배려적 사고 증진에 긍정적인 영향이 있는 것으로 나타났다. 둘째, 극화놀이를 활용한 공감교육은 유아의 창의적 인성 함양에 긍정적인 영향이 있는 것으로 나타났다. 결론적으로 본 연구는 극화놀이를 활용한 공감교육이 유아의 배려적 사고와 창의적 인성 증진을 위한 교육활동으로 가치가 있음을 입증하였다. 이를 통해 극화놀이의 교육적 가치와 함께 유아교육현장에서 극화놀이 활동의 효율적인 적용에 대한 기초자료로서 활용될 수 있음을 시사한다. This study examines the impact of empathy education using dramatic play on the considerate thinking and creative personality of young children. The research subjects 40 children aged 5 years old in D-early childhood education institutions in M region. The research tools used a considerate thinking scale and creative personality test tool. The experimental group provided empathy education using dramatic play and the comparative group provided empathy education through story sharing. The data analysis was analyzed through t-verification using SPSS24.0 to verify the effectiveness of the experimental treatment. Studies show that first, empathy education using dramatic play has a positive effect on the promotion of considerate thinking in young children. Second, empathy education using dramatic play has a positive effect on the promotion of creative character of young children. In conclusion, this study proved that empathy education using dramatic play is valuable as educational activities to promote considerate thinking and creative personality of young children. This suggests that it can be used as a basic data on the efficient application of the play activities in the field of early childhood education along with the educational value of the play.
자연친화 교육활동이 유아의 행복감 및 환경친화적 태도에 미치는 효과
홍지명 ( Jimyeong Hong ) 미래유아교육학회 2015 미래유아교육학회지 Vol.22 No.4
본 연구에서는 자연친화 교육활동이 유아의 행복감 및 환경친화적 태도에 어떠한 영향을 미치는지 알아보고자 하였다. 연구대상은 G시에 위치한 A어린이집의 만 5세 유아 46명으로 실험집단 23명과 비교집단 23명으로 실험집단은 자연친화 교육활동을 실시하였으며 비교집단은 일반적인 실외활동을 실시하였다. 연구결과, 자연친화 교육활동은 유아의 행복감 및 환경친화적 태도를 유의하게 증진시켰다. 이에 유아의 행복감 및 환경친화적 태도를 증진할 수 있는 교수-학습방법으로서의 자연친화 교육활동의 교육적 가치를 논하였다. 나아가 본 연구결과를 토대로 유아교육현장에서의 현장적용 가능성 및 자연친화 교육활동의 다양한 교육적 실천에 있어 이론적 기초자료로서의 활용성을 시사하였다. This study was to examine the effects of the nature friendly education activities on young children``s happiness and environmental friendly attitude. The subjects were 46 five-year-old young children in the A pre-school located in G city. The experimental group was carried out the nature friendly education activities. The results of this study were as follows: First, experimental group showed higher happiness than control group. Second, experimental group showed higher environmental friendly attitude than control group. Consequently, the nature friendly education activities promoted young children``s happiness and environmental friendly attitude. Moreover, the results of this study suggested that nature friendly education activities is worth utilizing as a proper and meaningful method to improve young children``s happiness and environmental friendly attitude.
반성적 모의수업이 예비유아교사의 교사효능감 및 실천지능에 미치는 영향
홍지명 ( Jimyeong Hong ) 사단법인 아시아문화학술원 2019 인문사회 21 Vol.10 No.6
예비유아교사의 수업 전문성 강화는 교육의 질을 위해 매우 중요하며, 모의수업은 교사 전문성을 향상시킬 수 있는 실질적인 방법 중 하나이다. 이에 본 연구는 반성적 모의수업이 예비유아교사의 교사효능감 및 실천지능에 미치는 영향을 살펴보는데 목적이 있다. 연구대상은 J지역의 S대학 유아교육과 2학년 총 35명이다. 연구도구는 교사효능감 및 실천지능 검사 도구를 사용하였다. 실험처치는 「아동미술」 교과목 수업에서 8주차에 걸쳐 반성적 모의수업을 계획하고 이를 실행, 평가하는 과정을 진행하였다. 연구결과, 반성적 모의수업은 예비유아교사의 교사효능감과 실천지능을 향상시켜 주는 것으로 밝혀졌다. 결론적으로 본 연구를 통해 반성적 사고를 증진하고 수업을 개선해 보는 반성적 모의수업은 예비유아교사들의 교사효능감의 발달뿐만 아니라 실천지능의 증진을 위한 효과적인 수업방법이 될 수 있음을 시사한다. 또한 예비유아교사의 수업 실행 능력 증진 방안 모색을 위한 기초자료로서 활용될 수 있음을 시사한다. Strengthening the teaching expertise of pre-service early childhood teacher is very important for the quality of education, and simulated instruction are one of the practical ways to improve teacher expertise. The aims of this study is to measured the influence of reflective simulated instruction on teaching efficacy and practical intelligence of pre-service early childhood teacher. The subjects of this study were 35 students in early childhood education at S university in J district. Research tools were used to examine teaching efficacy and practical intelligence. The experimental procedure consisted of planning, implementing, and evaluating reflective simulated instruction for a total of eight weeks in the “Children’s Art” course. As a result, it was found that the reflective simulated instruction positively influences the teaching efficacy and practical intelligence of the pre-service early childhood teacher. In conclusion, this study suggests that reflective simulated instruction can be an effective teaching methods for strengthening teaching efficacy and practical intelligence of pre-service early childhood teachers. It also suggests that it can be used as a basis for finding ways to improve the ability of pre-service early childhood teachers.
Kinetic Behavior of Salmonella on Low NaNO<sub>2</sub> Sausages during Aerobic and Vacuum Storage
Ha, Jimyeong,Gwak, Eunji,Oh, Mi-Hwa,Park, Beomyoung,Lee, Jeeyeon,Kim, Sejeong,Lee, Heeyoung,Lee, Soomin,Yoon, Yohan,Choi, Kyoung-Hee Korean Society for Food Science of Animal Resource 2016 한국축산식품학회지 Vol.36 No.2
This study evaluated the growth kinetics of Salmonella spp. in processed meat products formulated with low sodium nitrite (NaNO<sub>2</sub>). A 5-strain mixture of Salmonella spp. was inoculated on 25-g samples of sausages formulated with sodium chloride (NaCl) (1.0%, 1.25%, and 1.5%) and NaNO<sub>2</sub> (0 and 10 ppm) followed by aerobic or vacuum storage at 10℃ and 15℃ for up to 816 h or 408 h, respectively. The bacterial cell counts were enumerated on xylose lysine deoxycholate agar, and the modified Gompertz model was fitted to the Salmonella cell counts to calculate the kinetic parameters as a function of NaCl concentration on the growth rate (GR; Log CFU/g/h) and lag phase duration (LPD; h). A linear equation was then fitted to the parameters to evaluate the effect of NaCl concentration on the kinetic parameters. The GR values of Salmonella on sausages were higher (p<0.05) with 10 ppm NaNO<sub>2</sub> concentration than with 0 ppm NaNO<sub>2</sub>. The GR values of Salmonella decreased (p<0.05) as NaCl concentration increased, especially at 10℃. This result indicates that 10 ppm NaNO<sub>2</sub> may increase Salmonella growth at low NaCl concentrations, and that NaCl plays an important role in inhibiting Salmonella growth in sausages with low NaNO<sub>2</sub>.