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吴娇 ( Wu¸ Jiao ) 한국중국산문학회 2020 중국산문연구집간 Vol.10 No.-
In the history books of the Pre-Qin Dynasty, the records of ministers' outspoken criticism of the monarch can be traced back to the Shang and Zhou Dynasties, and appeared in large quantities in the Spring and Autumn Period. Different from the argument that the old ministers of the Western Zhou dynasty were always quoted with "the way of the former king", the admonishing ministers with complicated status in the Spring and Autumn Period prefer to pay special attention to quoting from the poem, analyzing current events and transmitting the voice of the people when criticizing the monarch, and deliberately used rhetorical skills and logical argumentation to improve the persuadability of the direct admonishing words. These characteristics showed that in the Spring and Autumn period, when blood ties were weakened, cultural identity and rhetoric level gradually became a new bond to build trust between Kings and ministers, and finally led to the formation of vertical and horizontal debates in the Warring States Period.
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Transcriptome Analysis of Antrodia cinnamomea Mycelia from Different Wood Substrates
Jiao-Jiao Chen,Zhang Zhang,Yi Wang,Xiao-Long Yuan,Juan Wang,Yu-Ming Yang,Yuan Zheng 한국균학회 2023 Mycobiology Vol.51 No.1
Antrodia cinnamomea, an edible and medicinal fungus with significant economic value and application prospects, is rich in terpenoids, benzenoids, lignans, polysaccharides, and benzo- quinone, succinic and maleic derivatives. In this study, the transcriptome of A. cinnamomea cultured on the wood substrates of Cinnamomum glanduliferum (YZM), C. camphora (XZM), and C. kanehirae (NZM) was sequenced using the high-throughput sequencing technology Illumina HiSeq 2000, and the data were assembled by de novo strategy to obtain 78,729 Unigenes with an N50 of 4,463 bp. Compared with public databases, about 11,435, 6,947, and 5,994 Unigenes were annotated to the Non-Redundant (NR), Gene Ontology (GO), and Kyoto Encyclopedia of Genes and Genome (KEGG), respectively. The comprehensive analysis of the mycelium terpene biosynthesis-related genes in A. cinnamomea revealed that the expression of acetyl-CoA acetyltransferase (AACT), acyl-CoA dehydrogenase (MCAD), 3- hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA), mevalonate pyrophosphate decarboxylase (MVD), and isopentenyl diphosphate isomerase (IDI) was significantly higher on NZM com- pared to the other two wood substrates. Similarly, the expression of geranylgeranyltransfer- ase (GGT) was significantly higher on YZM compared to NZM and XZM, and the expression of farnesyl transferase (FTase) was significantly higher on XZM. Furthermore, the expressions of 2,3-oxidized squalene cyclase (OCS), squalene synthase (SQS), and squalene epoxidase (SE) were significantly higher on NZM. Overall, this study provides a potential approach to explore the molecular regulation mechanism of terpenoid biosynthesis in A. cinnamomea.
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Zhang, Jiao Jiao,Kang, Tae Yoon,Kwon, Taeho,Koh, Hyebin,Chandimali, Nisansala,Huynh, Do Luong,Wang, Xian Zhong,Kim, Nameun,Jeong, Dong Kee American Society for Microbiology 2019 Infection and immunity Vol.87 No.3
<P><I>Gallibacterium anatis</I> is a pathogen associated with peritonitis and salpingitis in chickens and other avian species. Novel safety prevention strategies are urgently needed because of widespread multidrug resistance and antigenic diversity.</P><P><I>Gallibacterium anatis</I> is a pathogen associated with peritonitis and salpingitis in chickens and other avian species. Novel safety prevention strategies are urgently needed because of widespread multidrug resistance and antigenic diversity. The objective of this study was to produce a specific chicken egg yolk antibody and evaluate its protective response against a <I>G. anatis</I> infection model in 4-week-old chicks. Enzyme-linked immunosorbent assays showed that hens immunized with the recombinant N terminus of <I>Gallibacterium</I> toxin A (GtxA-N) had significantly increased antibody titers against GtxA-N in serum and egg yolk IgY. Western blotting showed that IgY antibody had specificity against GtxA-N in the egg yolks of immunized hens. The growth of <I>G. anatis</I> in brain heart infusion (BHI) broth and agar was significantly inhibited by the GtxA-N-specific IgY antibody. The protective effects of the specific IgY antibody were evaluated in <I>G. anatis</I>-infected chicks after intramuscular injection (10 mg/ml). The anti-GtxA-N antibody titers in the sera of <I>G. anatis</I>-challenged chicks following an injection of specific IgY antibody were significantly higher than those of the control and the nonspecific IgY groups, but lower lesion scores for the peritoneum, liver, and duodenum were found after specific IgY antibody treatment. The results from this study suggest that the GtxA-N-specific IgY antibody could potentially improve the protective response against <I>G. anatis</I> infection in chicks.</P>
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한국의 신문 기사에 나타난 중국 TV 프로그램명의 음역 양상
원교교 ( Jiao Jiao Yuan ),김정남 ( Jung Nam Kim ) 한국텍스트언어학회 2014 텍스트언어학 Vol.37 No.-
The article is concerning the method of marking Chinese TV programs. This study aims at figuring out an effective method of marking titles of Chinese TV programs by Korean characters. Currently, two common methods are employed by most writers of Korean newspaper industries. The one is the original sound of Chinese title from Regulations on Hangeul Transcription of Loan words. The other is traditional sound of Sino-Korean characters. The article conducts survey on how the two methods functioned during the recent three years, which demonstrates that the two methods are mixed in some circumstances. In conclusion, the article supports the original sound of the Chinese title as the method of making Chinese TV programs.
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Innovative Approach of Non-Thermal Plasma Application for Improving the Growth Rate in Chickens
Zhang, Jiao Jiao,Wang, Xian Zhong,Kwon, Taeho,Huynh, Do Luong,Chandimali, Nisansala,Kim, Nameun,Kang, Tae Yoon,Ghosh, Mrinmoy,Gera, Meeta,Lee, Sang Baek,Lee, Sung Jin,Lee, Wang Shik,Kim, Seong Bong,Mo MDPI 2018 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.19 No.8
<P>As an innovative technology in biological applications—non-thermal plasma technique—has recently been applied to living cells and tissues. However, it is unclear whether non-thermal plasma treatment can directly regulate the growth and development of livestock. In this study, we exposed four-day-incubated fertilized eggs to plasma at 11.7 kV for 2 min, which was found to be the optimal condition in respect of highest growth rate in chickens. Interestingly, plasma-treated male chickens conspicuously grew faster than females. Plasma treatment regulated the reactive oxygen species homeostasis by controlling the mitochondrial respiratory complex activity and up-regulating the antioxidant defense system. At the same time, growth metabolism was improved due to the increase of growth hormone and insulin-like growth factor 1 and their receptors expression, and the rise of thyroid hormones and adenosine triphosphate levels through the regulation of demethylation levels of growth and hormone biosynthesis-related genes in the skeletal muscles and thyroid glands. To our knowledge, this study was the first to evaluate the effects of a non-thermal plasma treatment on the growth rate of chickens. This safe strategy might be beneficial to the livestock industry.</P>
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Jiao, Ting,Lei, Zhaomin,Wu, Jianping,Li, Fei,Casper, David P.,Wang, Jianfu,Jiao, Jianxin Asian Australasian Association of Animal Productio 2021 Animal Bioscience Vol.34 No.11
Objective: This project aimed to evaluate the effects of both different additives and filling methods on nutritive quality, fermentation profile, and in situ digestibility of whole plant corn silage. Methods: Whole plant corn forage harvested at 26.72% dry matter (DM) was chopped and treated with two filling methods, i) fill silos at one time (F1), ii) fill silos at three times (F3), packing samples into one/three silo capacity at the first day, another one/three capacity at the second day, then one/three at the third day, three replicates. For each replicate, samples were treated with three additives, i) control (CTRL, no additive), ii) Sila-Max (MAX, Ralco Nutrition Inc., Marshall, MN, USA), and iii) Sila-Mix (MIX, Ralco Nutrition Inc., USA). With three replicates of each secondary treatment, there were nine silos, 54 silos in total. Each silo had a packing density of 137.61 kg of DM/m3. All silos were weighed and stored in lab at ambient temperature. Results: After 60 d of ensiling, all items showed good silage fermentation under MAX filled one time or three times (p<0.01). Higher silage quality for all additives was obtained at filling one time than that filled three times (p<0.01). The highest DM and lowest DM loss rate (DMLR) occurred to MAX treatment at two filling methods (p<0.01); Digestibility of acid detergent fiber, neutral detergent fiber (NDF), and curde protein had the same results as silage quality (p<0.01). Yield of digestible DM and digestible NDF also showed higher value under MAX especially for filling one time (p<0.05). Conclusion: All corn silages showed good fermentation attributes (pH<4.0). The forage filled one time had higher silage quality than that filled three times (p<0.01). MAX with homofermentative lactic acid bacteria enhanced the lactic acid fermentation, silage quality and nutrient digestibility, and so improved the digestible nutrient yield.
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Expression of the Pokemon Proto-oncogene in Nasopharyngeal Carcinoma Cell Lines and Tissues
Jiao, Wei,Liu, Fei,Tang, Feng-Zhu,Lan, Jiao,Xiao, Rui-Ping,Chen, Xing-Zhou,Ye, Hui-Lan,Cai, Yong-Lin Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.11
To study the differentiated expression of the proto-oncogene Pokemon in nasopharyngeal carcinoma (NPC) cell lines and tissues, mRNA and protein expression levels of CNE1, CNE2, CNE3 and C666-1 were detected separately by reverse transcription polymerase chain reaction (RT-PCR), real-time PCR and Western-blotting. The immortalized nasopharyngeal epithelial cell line NP69 was used as a control. The Pokemon protein expression level in biopsy specimens from chronic rhinitis patients and undifferentiated non keratinizing NPC patients was determined by Western-blotting and arranged from high to low: C666-1>CNE1>CNE2> CNE3>NP69. The Pokemon mRNA expression level was also arranged from high to low: CNE1>CNE2>NP69>C666-1>CNE3. Pokemon expression of NP69 and C666-1 obviously varied from mRNA to protein. The Pokemon protein level of NPC biopsy specimens was obviously higher than in chronic rhinitis. The data suggest that high Pokemon protein expression is closely associated with undifferentiated non-keratinizing NPC and may provide useful information for NPC molecular target therapy.
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A Partition-Based Active Contour Model Incorporating Local Information for Image Segmentation
Shi, Jiao,Wu, Jiaji,Paul, Anand,Jiao, Licheng,Gong, Maoguo Hindawi Publishing Corporation 2014 The Scientific World Journal Vol.2014 No.-
<P>Active contour models are always designed on the assumption that images are approximated by regions with piecewise-constant intensities. This assumption, however, cannot be satisfied when describing intensity inhomogeneous images which frequently occur in real world images and induced considerable difficulties in image segmentation. A milder assumption that the image is statistically homogeneous within different local regions may better suit real world images. By taking local image information into consideration, an enhanced active contour model is proposed to overcome difficulties caused by intensity inhomogeneity. In addition, according to curve evolution theory, only the region near contour boundaries is supposed to be evolved in each iteration. We try to detect the regions near contour boundaries adaptively for satisfying the requirement of curve evolution theory. In the proposed method, pixels within a selected region near contour boundaries have the opportunity to be updated in each iteration, which enables the contour to be evolved gradually. Experimental results on synthetic and real world images demonstrate the advantages of the proposed model when dealing with intensity inhomogeneity images.</P>
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