A Novel Alternaria Species Isolated from Peucedanum japonicum in Korea

( Jian Xin Deng ),( Hye Sun Cho ),( Narayan Chandra Paul ),( Hyang Burm Lee ),( Seung Hun Yu ) 한국균학회 2014 Mycobiology Vol.42 No.1

We isolated and examined a new Alternaria sp., which causes leaf spots on Peucedanum japonicum in Korea, by using molecular and morphological methods. Phylogenetic analysis based on a combined internal transcribed spacer region analysis andtwo protein-coding genes(gpd and Alt a1) demonstrated that the causal fungus was most closely related to A. cinerariae and A. sonchi, and relevant to A. brassicae. However, conidial morphology indicated that it is a novel species within the genus Alternaria, and therefore we have assigned the fungus a new name in this study.

Comparative Study on Single Pulse and Dual Pulses Variable Width Control Strategies for High-Speed PM BLDC Motor Drive

Jian-Xin Shen,Xue-Fei Qin,Wei-Zhong Fei,Yun-Chong Wang 한국자기학회 2019 Journal of Magnetics Vol.24 No.2

Pulse width modulation (PWM) of voltage source inverter (VSI) is common for speed regulation of permanent magnet (PM) brushless direct-current (BLDC) motor drives. However, when the motor runs at high speed, the conventional PWM technique may become unfeasible due to the low carrier ratio of inverter, which makes the motor current distorted and the motor core loss increased significantly. In this paper, two control strategies, namely single pulse variable width (SPVW) and dual pulses variable width (DPVW), are introduced for the high-speed PM BLDC motor drives, and are comprehensively investigated. Finite element method (FEM) is employed to evaluate and compare the drive system performance when using the conventional PWM, and the proposed SPVW and DPVW, respectively. Influence of the control techniques, especially on the loss distribution, is revealed.

Experimental study on cold-formed lipped channel stub columns reinforced by steel bars or steel strips

Jian-Feng Chen,Ming-Qi Chen,Yong-Kang Shen,Jian-Hua Shao,Xin-Xin Yang 국제구조공학회 2021 Steel and Composite Structures, An International J Vol.40 No.3

In this paper, the axial compressive properties of cold-formed steel lipped channel stub columns strengthened by rebars or steel strips are experimentally studied. The experiment included two sections. A kind of columns with local buckling is reinforced longitudinally by steel strips at the web. The other is the columns with distorted buckling, which is reinforced longitudinally by steel bars at the curling edge. The failure mode, deformation characteristics, ultimate bearing capacity and load displacement curve of the specimen are obtained through the experiment. On the basis of the experiment, the calculation results of theoretical axial bearing capacity of cold-formed steel lipped channel stub columns in Chinese, North American and Australian code are compared and analyzed. Research indicates: First, the cold-formed steel lipped channel stub columns strengthened by steel bars or steel strips can effectively improve the compressive bearing capacity of the specimen. Secondly, when the initial stress ratio of specimen is less than 0.3, the reinforcement effect is ideal. Thirdly, the three standards don’t stipulate the calculation of theoretical bearing capacity of the specimen strengthened after loading, so there is a large deviation between the theoretical calculation value and the test value.

First Report on Isolation of Penicillium adametzioides and Purpureocillium lilacinum from Decayed Fruit of Cheongsoo Grapes in Korea

Jian Xin Deng,Narayan Chandra Paul,상현규,Ji Hye Lee,황용수,유승헌 한국균학회 2012 Mycobiology Vol.40 No.1

Two species, Penicillium adametzioides and Purpureocillium lilacinum, were isolated from decayed grapes (cv. Cheongsoo) in Korea. Each species was initially identified by phylogenetic analysis of a combined dataset of two genes. Internal transcribed spacer (ITS) and β-tubulin (BT2) genes were used for identification of Penicillium adametzioides, and ITS and partial translation elongation factor 1-α (TEF) genes were used for identification of Purpureocillium lilacinum. Morphologically, they were found to be identical to previous descriptions. The two species presented here have not been previously reported in Korea.

OPE molecular junction as a hydrogen gas sensor

Jian-Guo Xin,Chuan-Lu Yang,Mei-Shan Wang,Xiao-Guang Ma 한국물리학회 2018 Current Applied Physics Vol.18 No.3

Oligo(phenylene ethynylene) (OPE) molecular junction has been suggested as a H2 molecule sensor based on calculations using the first principles of density–functional theory and non-equilibrium Green's function. The electronic transport properties of the OPE molecule between two Au electrodes with or without adsorbed H2 molecules are investigated. Results show that the adsorbed H2 molecule significantly changes the characteristics of the current–voltage curve of the OPE molecular junction. The pure OPE molecular junction exhibits a significant negative differential resistance, but this kind of phenomenon will disappear or weaken after hydrogen molecules are adsorbed. The conductance of the junction also obviously decreases in the bias range of [−0.4, 0.4] V after adsorbing H2 molecules. These effects can be used to design a H2 molecule sensor.

Alternaria brassicifolii sp. nov. Isolated from Brassica rapa subsp. pekinensis in Korea

( Jian Xin Deng ),( Mei Jia Li ),( Narayan Chandra Paul ),( May Moe Oo ),( Hyang Burm Lee ),( Sang-keun Oh ),( Seung Hun Yu ) 한국균학회 2018 Mycobiology Vol.46 No.2

A new species belonging to the genus Alternaria was isolated from the necrotic leaf spots of Brassica rapa subsp. pekinensis in Yuseong district, Daejeon, Korea. It is an occasional isolate, not an etiological agent, which is morphologically similar to A. broccoli-italicae, but differs in conidial size and conidiophore shape. Phylogenetic analysis using the sequence datasets of the internal transcribed spacer (ITS) region of the rDNA, glyceraldehyde-3-phosphate dehydrogenase (gpd), and plasma membrane ATPase genes showed that it is distantly related to A. broccoli-italicae and closely related to Alternaria species in the section Pseudoalternaria, which belonged to a clade basal to the section Infectoriae. Morphologically, the species is unique because it produces solitary conidia or conidial chains (two units), unlike the four members in the section Pseudoalternaria that produce conidia as short branched chains. It exhibits weak pathogenicity in the host plant. This report includes the description and illustration of A. brassicifolii as a new species.

Research on the Positioning of Work Machines in Greenhouse Using Bluetooth Low Energy Indoor Detection Technology

( Jian-xin He ),( Cang-qi Li ),( Ming-yen Lin ),( Chyung Ay ) 한국농업기계학회 2018 한국농업기계학회 학술발표논문집 Vol.23 No.1

Outdoor is using a mobile device combined with global positioning system (GPS) for positioning, the most popular application today; and indoor is based on the signal strength of Bluetooth, Wi-Fi, ZigBee and other wireless communication technology as a positioning basis. This research uses a fruit harvester for positioning in a greenhouse. However, the positioning accuracy of GPS in a greenhouse is easily affected and unable to be precise due to obstacles, communication channels, and multiple paths. To solve this problem, this study used iBeacon Bluetooth 4.0 module as a communication device for indoor positioning. In the experiment, ten iBeacons were used as the sending end, placed at various points in the room to serve as a positioning basis, and then installed an iBeacon combined with the Arduino on a fruit harvester as the receiving end. Then use the intensity of the sending and receiving signal as the basis, with the least square method, to calculate the corresponding distance between the signal receiving point and the sending point, and finally estimate the position of the fruit harvester by triangulation. At present, the preliminary experiment results show that, with the classroom as the test space, the indoor positioning success rate can reach 77% within 3 meters of the error value. The same method will be applied to find out the best positioning method for the harvester in the greenhouse.

Occurrence of Leaf Blight on Cosmos Caused by Alternaria cosmosa in Korea

Jian Xin Deng,이지혜,Narayan Chandra Paul,조혜선,Hyang Burm Lee,유승헌 한국식물병리학회 2015 Plant Pathology Journal Vol.31 No.1

In 2011, a leaf blight disease was observed on cosmos(Cosmos bipinnatus) leaves in Nonsan, Korea. Thecausal pathogen was isolated and identified based onmorphological and molecular approaches. Morphologicalcharacteristics of the pathogen matched well with theAlternaria cosmosa and also easily distinguishablefrom Alternaria zinniae reported from cosmos seedsby producing branched beak. Phylogenetically, thepathogen could not be distinguished from A. passifloraebased on the sequence analysis of a combined dataset of Alt a1 and gpd genes. However, A. passifloraewas distinguished from the present species by havingconidiophores with 4 to 5 conidiogenous loci. Theresults indicate that the present Alternaria species is A. cosmosa. Pathogenicity tests revealed that the isolatewas pathogenic to the leaves of Cosmos bipinnatus. Thisis the first report of Alternaria blight disease caused byA. cosmosa on cosmos in Korea.

Statistical Description and Analysis of the Concurrent Data Transmission from Massive MTC Devices

Xin Jian,Xiaoping Zeng,Jie Huang,Yunjian Jia,Yu Zhou 보안공학연구지원센터 2014 International Journal of Smart Home Vol.8 No.4

CircularRNA_104670 plays a critical role in intervertebral disc degeneration by functioning as a ceRNA

Jian Son,Hong-Li Wang,Ke-Han Song,Zhi-Wen Ding,Hai-Lian Wang,Xiao-Sheng Ma,Fei-Zhou Lu,Xin-Lei Xia,Ying-Wei Wang,Fei-Zou,Jian-Yuan Jiang 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

This study was carried out to explore the roles of circular RNAs (circRNAs) in nucleus pulposus (NP) tissues in intervertebral disc degeneration (IDD). Differentially expressed circRNAs in IDD and normal NP tissues were identified based on the results of microarray analysis. Bioinformatics techniques were employed to predict the direct interactions of selected circRNAs, microRNAs (miR), and mRNAs. CircRNA_104670 was selected as the target circRNA due to its large multiplier expression in IDD tissues. After luciferase reporter and EGFP/RFP reporter assays, we confirmed that circRNA_104670 directly bound to miR-17-3p, while MMP-2 was the direct target of miR-17-3p. The receiver-operating characteristic (ROC) curve showed that circRNA_104670 and miR-17-3p had good diagnostic significance for IDD (AUC circRNA_104670 = 0.96; AUC miRNA-17-3p = 0.91). A significant correlation was detected between the Pfirrmann grade and expression of circRNA_104670 (r = 0.63; p = 0.00) and miR-17-3p (r = −0.62; p = 0.00). Flow-cytometric analysis and the MTT assay showed that interfering with circRNA_104670 using small interfering RNA (siRNA) inhibited NP cell apoptosis (p < 0.01), and this inhibition was reduced by interfering with miR-17-3p. Interfering with circRNA_104670 suppressed MMP-2 expression and increased extracellular matrix (ECM) formation, which were also reduced by interfering with miR-17-3p. Finally, an MRI evaluation showed that circRNA_104670 inhibition mice had a lower IDD grade compared with control mice (p < 0.01), whereas circRNA_104670 and miRNA-17-3p inhibition mice had a higher IDD grade compared with circRNA_104670 inhibition mice (p < 0.05). CircRNA_104670 is highly expressed in the NP tissues of IDD and acts as a ceRNA during NP degradation.