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DNA methylation and mRNA expression of COL6A3 in antler mesenchyme of female and male reindeer
Jian‑Cheng Zhai,Ruo‑Bing Han,Sheng‑Nan Wang,Qiang‑Hui Wang,Yan‑Ling Xia,Wei‑Shi Liu,Ya‑Jie Yin,He‑Ping Li 한국유전학회 2019 Genes & Genomics Vol.41 No.9
Backgroud Reindeer is the only deer species that both male and female produce antlers, which provides a particularly interesting case in studying the differences between antlers of the two sexes. Alpha 3(VI) Collagen Gene (COL6A3), forms a microfibrillar network associated with the structural integrity and biomechanical properties, has been found to be one of the differentially expressed genes in antler mesenchyme of female and male reindeer. Objective and Methods The promoter sequence of reindeer COL6A3 gene was obtained using the cloning technology and analyzed by the bioinformatics methods. Bisulfite sequencing PCR (BSP) was used to detect the methylation status of the COL6A3 promoter in reindeer antler mesenchyme. Real-time quantitative PCR was used to detect COL6A3 expression in the antler mesenchyme of female and male reindeer. Results Sequence analysis revealed that the reindeer COL6A3 partial promoter sequence was 983 bp including the possible promoter region at + 105 bp to + 155 bp. Homology and phylogenetic analysis indicated that the COL6A3 promoter of reindeer had the closest genetic distance with Bos taurus, Capra hircus and Ovis aries. BSP results indicated that the methylation level of COL6A3 promoter in the female reindeer antler mesenchyme was significantly higher than in the male. Correlating with increased methylation status, we also found that COL6A3 mRNA expression in female reindeer antler mesenchyme was significantly lower than in the male. Conclusion The higher methylation level of the COL6A3 gene in female reindeer antler mesenchyme coincides with decreased COL6A3 mRNA expression, thereby affecting the transposon silencing mechanism and possibly contributing to apparent differences of antlers in female and male reindeer.
Qiang Li,Yi-ming Fang,Jian-Xiong Li,Wen-jian Zhang 제어·로봇·시스템학회 2020 International Journal of Control, Automation, and Vol.18 No.12
In view of the fact that the vibration displacement system of continuous casting mold driven by a servo motor requires the motor rotate in a certain fixed direction, a compound control scheme combining feedforward and feedback control is proposed in this paper. In controller designing, there are mainly two issues to be considered: i) constraint condition that the servo motor rotates in a fixed direction while the mold vibrates in a periodic form. ii) uncertainties caused by time-varying load and mechanical factors, such as mechanical zero initial deviation and machining precision. As to the system uncertainties and the periodic vibration displacement of mold, proportional-integral (PI) controller combined with the repetitive control method are used to realize displacement tracking and improve the tracking performance. A feedforward control algorithm consisting of the reference mold displacement and its first derivative is adopted to solve the constraint condition. Finally, some simulation results show the feasibility of the proposed algorithm for the constraint condition and system uncertainties. Additionally, a typical industry mold vibration system driven by a servo motor is conducted to show the effectiveness of the proposed control scheme.
Isolation of L-theanine from Tea Solution by Cation Exchange Resin in Batch and Fixed Bed Column
Jian-Hui Ye,Yi-Wen Luo,Hui-Ling Liang,Jian-Liang Lu,Jing Jin,Yue-Rong Liang,Xin-Qiang Zheng,Xian-Yang Luo 한국생물공학회 2011 Biotechnology and Bioprocess Engineering Vol.16 No.2
L-Theanine, a bioactive compound in tea, was isolated from tea solution using cation exchange resin no.732. The adsorption of L-theanine by cation exchange resin no.732 fit the Langmuir isotherm model and was a monolayer molecular interaction process. Thermodynamic studies revealed that the adsorption of L-theanine by resin no.732 was an exothermic and spontaneous physically driven process. The adsorption capacity was influenced by temperature, initial concentration, and pH. The L-theanine adsorption capacity under conditions at room temperature,pH 4.73, and initial L-theanine concentration 18 g/L was 241.731 ± 3.679 mg/g. The Thomas model was fit to describe the column adsorption data at different flow rates and initial concentrations. The L-theanine adsorbed by resin no.732 could be desorbed by 0.134 mol/L Na2HPO4aqueous solution with a recovery rate of 84.96%. These findings indicate that resin no.732 was a promising material for isolating L-theanine from tea solution.
Qiang Jian,Gang Song,Zhenlei Liu,Wanru Duan,Jian Guan,Fengzeng Jian,Zan Chen 대한척추신경외과학회 2022 Neurospine Vol.19 No.1
Objective: This study aimed to illustrate the features of fistula location distribution, surgical strategies, and outcomes in spinal epidural meningeal cysts (SEMCs). Methods: The authors searched and reviewed the medical records for cases of SEMCs. Imaging features, operative reports, and media were reviewed to accurately describe the surgical techniques employed. We recorded the level and laterality of the fistula according to the operative report and the media. Consistency analysis was performed on the dominant laterality of the cyst on preoperative axial magnetic resonance imaging and laterality of the fistula in the operative media or report. When cyst and fistula lateralities were the same, they were considered consistent. Finally, the Japanese Orthopedic Association (JOA) score was used to obtain patient-reported results at each follow-up. Results: Thirty patients with SEMCs were identified. Fistula repair was performed in all patients. Two patients experienced cyst recurrence after surgery and were repaired during the second surgery. Based on imaging findings, SEMCs mostly occurred in the thoracolumbar junction. Most of the fistulas (87.88%) were at the T12 or L1 levels. In patients with multiple adjacent SEMCs, the fistula may be at one end of the cyst rather than in the middle level of the cyst. A fistula laterality of 72.72% was consistent with cyst laterality. The JOA improvement rate was 61.84% ± 26.63%. Conclusion: Most fistulas were always located at the T12–L1 level as well as the middle level of the cyst, which is always consistent with cyst laterality. In patients with multiple adjacent SEMCs, the fistula may be at one end of the cyst. Cleft closure is key to healing SEMCs.
Reconstruction of the Cervical Lateral Mass Using 3-Dimensional-Printed Prostheses
Qiang Jian,Zhenlei Liu,Wanru Duan,Jian Guan,Fengzeng Jian,Zan Chen 대한척추신경외과학회 2022 Neurospine Vol.19 No.1
Objective: This study aimed to investigate the outcome of using 3-dimensional (3D)-printed prostheses to reconstruct a cervical lateral mass to maintain cervical stability. Methods: We retrospectively analyzed data of 7 patients who underwent cervical lateral mass reconstruction using a 3D-printed prosthesis, comprising axial and subaxial lateral mass reconstruction in 2 and 5 patients, respectively. Bilateral mass was reconstructed in 1 patient and unilateral mass in the remaining 6 patients. Results: Using a 3D-printed lateral mass prosthesis, internal fixation was stable for all 7 patients postoperatively. No implant-related complications such as prosthesis loosening, displacement, and compression were observed at the last follow-up. Conclusion: Reconstruction of the lateral mass structure is beneficial in restoring load transfer in the cervical spine under physiological conditions. A 3D-printed prosthesis can be considered a good option for reconstruction of the lateral mass as fusion was achieved, with no subsequent complications observed.
Fen-Qiang Li,Jun Zhao,Jian-Hua Mo,Jian-Jun Li,Liang Huang 대한기계학회 2017 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.31 No.3
In order to reveal the high-velocity deformation mechanisms of Ti-6Al-4V titanium alloy sheets, the dynamic deformation behavior and the microstructure evolutions were compared with those under quasi-static case employing Scanning electron microscopy/Electron back-scattered diffraction (SEM/EBSD) and Transmission electron microscopy (TEM). The results indicated that the distribution of microhardness was uneven. The cause was determined to be the non-uniform material flow and dynamic loading. The grain sizes of the Ti-6Al-4V titanium alloy sheet were nearly the same under two conditions, but the grains were elongated under the dynamic loading compared with that observed in quasi-static forming. In high-velocity bulging, dislocation slip was the principle mechanism of plastic deformation of Ti-6Al-4V titanium alloy when deformation was small. As the strain increased, the twinning phenomenon was activated in addition to the dislocation cell structure. The twinning plane was determined to be the (10 11) plane.
Apply Partition Tree to Compute Canonical Labelings of Graphs
HAO Jian-Qiang,GONG Yun-Zhan,Tan Li,Duan Da-Gao 보안공학연구지원센터 2016 International Journal of Grid and Distributed Comp Vol.9 No.5
This paper establishes a theoretical framework by defining a set of concepts useful for classifying graphs and computing the canonical labeling Cmax(G) of a given undirected graph G, which including the partition tree PartT(G), maximum partition tree MaxPT(G), centre subgraph Cen(G), standard regular sequence SRQ(G), standard maximum regular sequence SMRQ(G), and so on. The implementations of algorithms 1 to 5 show how to calculate them accordingly. The worst time complexities of algorithms 1, 2, 4, and 5 are O(n2) respectively. The time complexity of Algorithm 3 is O(n). By Theorem 3, all leaf nodes of PartT(G) and MaxPT(G) are the regular subgraphs. By Theorem 4 and 5, there exists only one Cen(G) in G. Regular Partition Theorem 6 shows that there exists just one corresponding PartT(G), SRQ(G), MaxPT(G), and SMRQ(G). One can use Classification Theorem 7 to category graphs. Theorem 8 and 9 establish the link between the Cen(G) and the calculation of the first node u1 added into MaxQ(G) corresponding to the canonical labeling Cmax(G) of G. Further, it utilizes the Cen(G) to calculate the first node u1 added into MaxQ(G). The proposed methods can be extended to deal with the directed graphs and weighted graphs.