MiR-421 Regulates Apoptosis of BGC-823 Gastric Cancer Cells by Targeting Caspase-3

Wu, Jian-Hong,Yao, Yong-Liang,Gu, Tao,Wang, Ze-You,Pu, Xiong-Yong,Sun, Wang-Wei,Zhang, Xian,Jiang, Yi-Biao,Wang, Jian-Jun Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.13

MicroRNAs might act as oncogenes or tumor suppressors in cancer. Recent studies have shown that miR-421 is up-regulated in human gastric cancer. Here, we found that miR-421 was over-expressed in gastric cancer tissues and cell lines. Bioinformatics analysis predicted that the caspase-3 gene was a target of miR-421. Caspase-3 was negatively regulated by miR-421 at the post-transcriptional level. Bax and Bcl-2 were also regulated by miR-421. Moreover, tumor necrosis factor receptor-I and -II, death receptors in the apoptosis pathway, were up-regulated by miR-421. The over-expression of miR-421 promoted gastric cancer cell growth and inhibited apoptosis of the BGC-823 gastric cancer cell line. These observations indicate that miR-421 acts as a tumor promoter by targeting the caspase-3 gene and preventing apoptosis of gastric cancer cells through inhibition of caspase-3 expression. These findings contribute to our understanding of the functions of miR-421 in gastric cancer.

Relationship Between the Prohibitin 3' Untranslated Region C > T Gene Polymorphism and Cancer Susceptibility - Results of a Meta-analysis

Zhou, Tian-Biao,Yin, Sheng-Sheng,Huang, Jian-Jian,Ou, Chao Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.7

Objective: The results from the published studies on the association between prohibitin 3' untranslated region C > T gene polymorphism and cancer risk are conflicting. This meta-analysis was performed to evaluate the relationship with cancer susceptibility overall, and to explore whether the T allele or TT genotype could become a predictive marker for cancer risk. Methods: Association studies were identified from the databases of PubMed, Embase, and Cochrane Library as of March 1, 2012, and eligible investigations were synthesized using the meta-analysis method. Results were expressed with odds ratios (OR) for dichotomous data, and 95% confidence intervals (CI) were also calculated. Results: Six investigations were identified for the analysis of association between the prohibitin 3' untranslated region C > T gene polymorphism and cancer risk, covering of 1,461 patients with cancer and 1,197 controls. There was a positive association between the T allele and cancer susceptibility (OR=1.20, 95% CI: 1.03-1.39, P=0.02), and CC homozygous might play a protective role (OR=0.80, 95% CI: 0.68-6.11, P=0.95). In the sub-group analysis, prohibitin 3' untranslated region C > T gene polymorphism and cancer risk appeared associated with the risk of breast cancer, but not ovarian cancer. Conclusions: Our results indicate that T allele is a significant genetic molecular marker to predict cancer susceptibility and CC genotype is protective, especially for breast cancer. However, more investigations are required to further clarify the association of the prohibitin 3' untranslated region C > T gene polymorphism with cancer susceptibility.

Separation Technology, Thermodynamics : Letters to The Editor ; Comments on Special Distillation Processes

( Jian Wei Li ),( Zhi Gang Lei ),( Cheng Yue Li ),( Biao Hua Chen ) 한국화학공학회 2005 Korean Journal of Chemical Engineering Vol.22 No.4

Radio-Sensitization by Piper longumine of Human Breast Adenoma MDA-MB-231 Cells in Vitro

Yao, Jian-Xin,Yao, Zhi-Feng,Li, Zhan-Feng,Liu, Yong-Biao Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.7

Background: The current study investigated the effects of Piper longumine on radio-sensitization of human breast cancer MDA-MB-231 cells and underlying mechanisms. Materials and Methods: Human breast cancer MDA-MB-231 cells were cultured in vitro and those in logarithmic growth phase were selected for experiments divided into four groups: control, X-ray exposed, Piper longumine, and Piper longumine combined with X-rays. Conogenic assays were performed to determine the radio-sensitizing effects. Cell survival curves were fitted by single-hit multi-target model and then the survival fraction (SF), average lethal dose ($D_0$), quasi-threshold dose ($D_q$) and sensitive enhancement ratio (SER) were calculated. Cell apoptosis was analyzed by flow cytometry (FCM). Western blot assays were employed for expression of apoptosis-related proteins (Bc1-2 and Bax) after treatment with Piper longumine and/or X-ray radiation. The intracellular reactive oxygen species (ROS) level was detected by FCM with a DCFH-DA probe. Results: The cloning formation capacity was decreased in the group of piperlongumine plus radiation, which displayed the values of SF2, D0, Dq significantly lower than those of radiation alone group and the sensitive enhancement ratio (SER) of D0 was1.22 and 1.29, respectively. The cell apoptosis rate was increased by the combination treatment of Piper longumine and radiation. Piper longumine increased the radiation-induced intracellular levels of ROS. Compared with the control group and individual group, the combination group demonstrated significantly decreased expression of Bcl-2 with increased Bax. Conclusions: Piper longumine at a non-cytotoxic concentration can enhance the radio-sensitivity of MDA-MB-231cells, which may be related to its regulation of apoptosis-related protein expression and the increase of intracellular ROS level, thus increasing radiation-induced apoptosis.

Combined effects of three novel SNPs within goat LHX3 gene on milk performance

Jin-Biao Liu,Chu-Zhao Lei,Xian-Yong Lan,Yao Xu,Zhuan-Jian Li,Hong Chen 한국유전학회 2011 Genes & Genomics Vol.33 No.5

In this study, single nucleotide polymorphisms (SNPs) of LHX3 gene were detected by DNA sequencing based on DNA pools and PCR-RFLP method in 792 goats belonging to three Chinese indigenous breeds (Guanzhong dairy, Xinong Sannen dairy, Inner Mongolia white cashmere). The results revealed three novel mutations (AY923832:g.7778A>T; g.8035T>C;g.10592C>T), which were named as P2-DraI, P3-HinfI and P4-MspI loci, respectively. The linkage disequilibrium analysis demonstrated P3-HinfI and P4-MspI loci were strongly linked (r^2>0.33) in all the analyzed populations. Each SNP produced no significant (p>0.05) effects on milk performance. However,the two-loci and three–loci combined genotypes had more profound impacts on milk yield than in separation. The individuals with diplotype AATT (P2-DraI/P3-HinfI) showed significantly (p<0.05) higher milk yield than those with diplotypes ATTT, TTTT, ATTC, and AACC. Diplotype TTCCTT (P2-DraI/P3-HinfI/P4-MspI) carriers had significantly (p<0.05) higher milk yield than those with diplotypes ATTTCC and AACCTC. These combined effects of LHX3 gene polymorphisms indicated that this gene had significant effect on milk performance and its corresponding combined diplotypes could be regarded as potential genetic markers of milk performance.

COORDINATED COLLISION AVOIDANCE FOR CONNECTED VEHICLES USING RELATIVE KINETIC ENERGY DENSITY

Manjiang Hu,Jian Wu,Hongmao Qin,Yougang Bian,Biao Xu,Qing Xu,Jugang He,Jianqiang Wang 한국자동차공학회 2017 International journal of automotive technology Vol.18 No.5

Vehicular collision often leads to serious casualties and traffic congestion, and the consequences are worse for multiple-vehicle collision. Many previous works on collision avoidance have only focused on the case for two consecutive vehicles using on-board sensors, which ignored the influence on upstream traffic flow. This paper proposes a novel coordinated collision avoidance (CCA) strategy for connected vehicles, which has potential to avoid collision and smooth the braking behaviors of multiple vehicles, leading to an improvement of traffic smoothness. Specifically, model predictive control (MPC) framework is used to formulate the CCA into an optimization problem, where the objective is to minimize the total relative kinetic energy density (RKED) among connected vehicles. Monte Carlo simulations are used to demonstrate the effectiveness of proposed CCA strategy by comparison with other two strategies. Among all the three control strategies, the RKED based control strategy shows the best performance of collision avoidance, including the best crash prevention rates (99.2 % on dry asphalt road and 90.5 % on wet asphalt road) and the best control of distance headways between vehicles.

Evaluation of Liver Metastases Using Contrast-Enhanced Ultrasound: Enhancement Patterns and Influencing Factors

( Wen Tao Kong ),( Zheng Biao Ji ),( Wen Ping Wang ),( Hao Cai ),( Bei Jian Huang ),( Hong Ding ) 대한소화기학회 2016 Gut and Liver Vol.10 No.2

Background/Aims: To evaluate the enhancement patterns of liver metastases and their influencing factors using dynamic contrast-enhanced ultrasound (CEUS). Methods: A total of 240 patients (139 male and 101 female; 58.5±11.2 years of age) diagnosed with liver metastases in our hospital were enrolled in this study to evaluate tumor characteristics using CEUS. A comparison of enhancement patterns with tumor size and primary tumor type was performed using the chi-square test. The differences between quantitative variables were evaluated with the independent-sample t-test and one-way analysis of variance. Results: The enhancement patterns of liver metastases on CEUS were categorized as diffuse homogeneous hyperenhancement (133/240, 55.4%), rim-like hyperenhancement (80/240, 33.3%), heterogeneous hyperenhancement (10/240, 4.2%), and isoenhancement (17/240, 7.1%). There were significant differences in the enhancement patterns during the arterial phase based on the nodule size (p=0.001). A total of 231 of the nodules showed complete washout during the portal phase, and 237 nodules were hypoenhanced during the delayed phase. The washout time was correlated with tumor vascularity, with a longer washout time observed in hypervascular metastases compared to hypovascular metastases (p=0.033). Conclusions: Diffuse homogeneous hyperenhancement followed by rapid washout was the most common enhancement pattern of liver metastases on CEUS and was affected by the nodule size and tumor vascularity. Small metastases were prone to show diffuse homogeneous hyperenhancement. Hypervascular metastases showed a significantly longer washout time compared to hypovascular metastases. (Gut Liver 2016;10:283-287)

Separation Technology, Thermodynamics : Improvement of separation process of synthesizing MIBK by the isopropanol one-step method

( Zhi Gang Lei ),( Jian Wei Li ),( Cheng Yue Li ),( Biao Hua Chen ) 한국화학공학회 2006 Korean Journal of Chemical Engineering Vol.23 No.2

Integrin-linked Kinase Functions as a Tumor Promoter in Bladder Transitional Cell Carcinoma

Wang, De-Lin,Lan, Jian-Hua,Chen, Liang,Huang, Biao,Li, Zeng,Zhao, Xiu-Min,Ma, Qiang,Sheng, Xia,Li, Wen-Bin,Tang, Wei-Xue Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.6

The aim of this study was to elucidate the role of the integrin-linked kinase (ILK) gene in development of human bladder transitional cell carcinoma (BTCC). Expression of ILK protein and ILK mRNA in 56 cases of human BTCC tissue and in 30 cases of adjacent normal bladder tissue was detected by immunohistochemistry S-P and reverse transcription polymerase chain reaction (RT-PCR), respectively. Four specific miRNA RNAi vectors targeting human ILK were synthesized and transfected into BIU-87 cells by liposome to obtain stable expression cell strains. The influence of ILK on proliferation of BTCC was detected by MTT, FCM on athymic mouse tumorigenesis. The positive rate of ILK protein in BTCC tissue (53.6%) was much higher than adjacent normal bladder tissue (10.0%) (p<0.05). Similarly, expression of ILK mRNA in BTCC tissue ($0.540{\pm}0.083$) was significantly higher than in adjacent normal bladder tissue ($0.492{\pm}0.070$) (p<0.05). MTT showed that the proliferation ability of miRNA-ILK transfected group was clearly decreased (p<0.05), the cell cycle being arrested in G0/G1-S, an tumorigenesis in vivo was also significantly reduced (p<0.05). ILK gene transcription and protein expression may be involved in the development of BTCC, so that ILK might be the new marker for early diagnosis and the new target for gene treatment.

Experimental and analytical study on RC beam reinforced with SFCB of different fiber volume ratios under flexural loading

Jia-Xiang Lin,Yong-Jian Cai,Ze-Ming Yang,Shu-Hua Xiao,Zhan-Biao Chen 국제구조공학회 2022 Steel and Composite Structures, An International J Vol.45 No.1

Steel fiber composite bar (SFCB) is a novel type of reinforcement, which has good ductility and durability performance. Due to the unique pseudo strain hardening tensile behavior of SFCB, different flexural behavior is expected of SFCB reinforced concrete (SFCB-RC) beams from traditional steel bar reinforced concrete (S-RC) beams and FRP bar reinforced concrete (F-RC) beams. To investigate the flexural behavior of SFCB-RC beam, four points bending tests were carried out and different flexural behaviors between S/F/SFCB-RC beams were discussed. An flexural analytical model of SFCB-RC beams is proposed and proved by the current and existing experimental results. Based on the proposed model, the influence of the fiber volume ratio R of the SFCB on the flexural behavior of SFCB-RC beams is discussed. The results show that the proposed model is effective for all S/F/SFCB-RC flexural members. Fiber volume ratio R is a key parameter affecting the flexural behavior of SFCB-RC. By controlling the fiber volume ratio of SFCB reinforcements, the flexural behavior of the SFCB-RC flexural members such as bearing capacity, bending stiffness, ductility and repairability of SFCB-RC structures can be designed.