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Yi‑Chia Kuan,Venkatesan Thiruvengadam,Jia‑Shin Lin,Jia‑Hsin Liu,Tsan‑Jan Chen,Hsin‑Mao Wu,Wen‑Ching Wang,Liang‑Jwu Chen 한국식물생명공학회 2018 Plant biotechnology reports Vol.12 No.1
The broad-specificity amino acid racemase (Bsar) from Pseudomonas putida catalyzes the racemization of various amino acids, offering a flexible and feasible platform to develop a new non-antibiotic selectable marker system for plant transformation. In the present study, we demonstrated that a Bsar variant, Bsar-R174K, that is useful as a selectable marker gene in Arabidopsis and rice that were susceptible to l-lysine and D-alanine. The introduction of wild-type Bsar, Bsar-R174K or Bsar-R174A into E. coli lysine or asparagine auxotrophs was able to rescue the growth of these microorganisms in minimal media supplemented with selectable amino acid enantiomers. The transformation of Arabidopsis with Bsar or Bsar variants based on d-alanine selection revealed that Bsar-R174K had the greatest efficiency (2.40%), superior to kanamycin selectionbased transformation (1.10%). Whereas, l-lysine-based selection exhibited lower efficiency for Bsar-R174K (0.17%). The progenies of selected Bsar-R174K transgenic Arabidopsis revealed normal growth properties. In addition, Bsar-R174K transgenic rice was obtained on l-lysine medium with an efficiency of 0.9%, and the progenies of the transgenic rice revealed morphologically normal phenotypes comparable with their wild-type counterparts. This study presents the first report of broad range amino acid racemase Bsar-R174K as a non-antibiotic selectable marker system applied in transgenic plants.
( Jia Yi Yu ),( Xiao Fei Zhou ),( Mi Kyoung Chang ),( Mako Nakaya ),( Jae Hoon Chang ),( Yi Chuan Xiao ),( J. William Lindsey ),( Stephanie Dorta Estremera ),( Wei Cao ),( Anna Zal ),( Tomasz Zal ),( 영남대학교 약품개발연구소 2015 영남대학교 약품개발연구소 연구업적집 Vol.25 No.-
Development of an immune or autoimmune response involves T-cell activation in lymphoid organs and subsequent migration to peripheral tissues. Here we show that T-cell-specific ablation of the kinase TBK1 promotes T-cell activation but causes retention of effector T cells in the draininglumph node in a neuroinflammatory autoimmunity model,experimental autoimmune encephalomyelitis (EAE).Atolder ager,the T-cell-conditional TBK1-knockout mice also spontaneously accumulate T cells with activated phenotype.TBK1 contrlos the activation of AKT and its downsream kinase m TIORC1 by a mechanism involving TBK1-stimulated AKT ubiquitination and degradation. The deregulated AKT-mTORC1 signalling in turn contributes to enhanced T-cell activation and impaired effector T-cell egress from draining lymph nodes. Treatment of mice with a small-molecule inhibitor fo TBK1 inhibits EAE induction. These results suggest a role for TBK1 in regulating T-cell migration and establish TBK1 as a regulator of the AKT-mTORC1 signalling axis.
Jia-Yi Yeh 국제구조공학회 2011 Smart Structures and Systems, An International Jou Vol.8 No.5
The parametric resonance problems of axisymmetric sandwich annular plate with an electrorheological (ER) fluid core and constraining layer are investigated. The annular plate is covered an electrorheological fluid core layer and a constraining layer to improve the stability of the system. The discrete layer annular finite element and the harmonic balance method are adopted to calculate the boundary of instability regions for the sandwich annular plate system. Besides, the rheological property of an electrorheological material, such as viscosity, plasticity, and elasticity can be changed when applying an electric field. When the electric field is applied on the sandwich structure, the damping of the sandwich system is more effective. Thus, variations of the instability regions for the sandwich annular plate with different applying electric fields, thickness of ER layer, and some designed parameters are presented and discussed in this study. The ER fluid core is found to have a significant effect on the location of the boundaries of the instability regions.
Jia-Yi Yeh 국제구조공학회 2014 Smart Structures and Systems, An International Jou Vol.13 No.1
The axisymmetric dynamic instability of polar orthotropic sandwich annular plate combinedwith electrorheological (ER) fluid core layer and constraining layer are studied in this paper. And, the ERcore layer and constraining layer are used to improve the stability of the annular plate system. Theboundaries of instability regions for the polar orthotropic sandwich annular plate system are obtained bydiscrete layer annular finite element and the harmonic balance method. The rheological property of anelectrorheological material, such as viscosity, plasticity, and elasticity can be controlled by applying differentelectric field strength. Thus, the damping characteristics of the sandwich system are more effective when theelectric field is applied on the sandwich structure. Additionally, variations of the instability regions for thepolar orthotropic sandwich annular plate with different applying electric field strength, thickness of ER layerand some designed parameters are investigated and discussed in this study.
An integrated dendrite-free zinc metal electrode for corrosion inhibition in aqueous system
Yi-Fan Hu,Li-Feng Zhou,He Gong,He Jia,Peng Chen,Yi-Song Wang,Li-Ying Liu,Tao Du 한국화학공학회 2022 Korean Journal of Chemical Engineering Vol.39 No.9
Zinc ion batteries have gotten increasing attention as a potential candidate for lithium-ion batteries, due totheir high specific capacity (820 mAh·g1), energy density, and safety. Inevitably, dendrite and corrosion create sometrouble for this system. Herein, an integrated Zn electrode coated by Zn-Al metal oxides prepared by a simple spincoatingmethod was utilized to increase the rechargeability for aqueous zinc ion batteries. By coating the Zn anodewith an artificial electrolyte interface, the wettability of Zn anodes was improved and impedance was reduced. Thecoating suppressed not only the appearance of dendrite but also the formation of corrosion products. The symmetricalcells with coating have a low overpotential (43mV) and an excellent life span. Meanwhile, the applied full batteriesexhibit an improved capacity retention rate (86.67% after 120 cycles), great rate performance and low apparent activationenergy (24.6 KJ·mol1). The simple production methods and superior corrosion suppression effects provide newideas for the anode protection of aqueous system batteries.
Yi-Xin Zou,Jia Qiao,Hua-Yuan Zhu,Rui-Nan Lu,Yi Xia,Lei Cao,Wei Wu,Hui Jin,Wen-Jie Liu,Jin-Hua Liang,Jia-Zhu Wu,Li Wang,Lei Fan,Wei Xu,Jian-Yong Li 대한암학회 2019 Cancer Research and Treatment Vol.51 No.2
Purpose Chronic lymphocytic leukemia (CLL) is one of the most frequent type of B-cell chronic lymphoproliferative disorders and chronic inflammation takes part in the development of CLL. However, there has been no valid immune biomarker to predict the prognosis of untreated CLL patients. Materials and Methods In this retrospective study, we analyzed the clinical correlations and prognostic value of albumin-to-fibrinogen ratio (AFR) detected at diagnosis in 191 CLL patients. Results The cut-off value of AFR was 9.7 calculated by X-tile. Patients who were more than 65 years old were often accompanied by low level of AFR (p < 0.001). Survival analysis showed that patients with low level of AFR had shorter overall survival (OS) than patients with high level of AFR (p < 0.001). Multivariate analysis illustrated that AFR had a negative impact on OS (p=0.003) and was independent of parameters involved in CLL international prognostic index and other prognostic markers such as CD38 and ZAP-70. Conclusion These data provide a comprehensive view of AFR and shows that AFR at diagnosis is an adverse prognostic factor in untreated CLL patients.
Jia, Wei-Juan,Jia, Hai-Xia,Feng, Hui-Yi,Yang, Ya-Ping,Chen, Kai,Su, Feng-Xi Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.1
Purpose: The purpose of this study was to investigate the recurrence pattern and characteristics of patients based on the 2013 St. Gallen surrogate molecular subtypes after breast-conserving surgery (BCS) in Chinese women. Methods: This retrospective analysis included 709 consecutive breast cancer patients undergoing BCS from 1999-2010 at our institution. Five different surrogate subtypes were created using combined expression of the estrogen receptor, progesterone receptor, and human epidermal growth factor receptor-2. Locoregional relapse-free survival (LRRFS), distant metastasis-free survival (DMFS), and disease-free survival (DFS) rates were calculated. Results: The 5-year LRRFS, DMFS, and DFS rates were 90.5%, 88.2%, and 81.5%, respectively. Multivariate analysis revealed that young age, node-positive disease, and HER2 enrichment were independent prognostic factors in LRRFS patients. There was also an independent prognostic role of lymph node-positive disease in DMFS and DFS patients. Patients with luminal A tumors had the most favorable prognosis, with LRRFS, DMFS, and DFS rates of 93.2%, 91.5%, and 87.4% at 5 years, respectively. Conversely, HER-2-enriched tumors exhibited the highest rate of locoregional recurrence (20.6%). Conclusion: Surrogate subtypes present with significant differences in RFS, DMFS, and LRRFS. Luminal A tumors have the best prognosis, whereas HER2-enriched tumors have the poorest.