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( Jhen-jia Guo ),( Ching-lu Hsieh ),( Ming-hung Chu ),( Chia-sheng Chen ) 한국농업기계학회 2018 한국농업기계학회 학술발표논문집 Vol.23 No.1
Hay quality is an important factor that affects yield and quality of animal products. It also influences its market price. Therefore, it is crucial to have quantitative value on hay quality. Conventionally hay quality is judged manually by its appearance, smell, and chemical composition that needs tedious laboratory analysis. Spectroscopy in near infrared, digital image technique, and electronic smell senor are techniques that have been broadly studied in agricultural field. They contain many advantages, such as, simple preparation for samples, non-destructive on samples, and quick detection. This study applied these three techniques in hay quality evaluation. Seventy Pangola hay samples were randomly collected from Kenting, a southern town in Taiwan. Each sample was scanned by a monochrometer (microPHAZER) to record its reflective spectrum at 1600~2400 nm. Its digital color image was taken by a commercial camera in a small chamber after a calibration process was performed. And the response curve of electric resistance change detected by three SiO<sub>2</sub> gas sensors of MQ5, TGS800, and TGS 822 were recorded for 15 minutes. These records were used to calibrate a prediction/classification model for hay quality evaluation. Among these sample data, 2/3 of them were used as calibration set while the rest were used as test set. For spectroscopy to predict composition, PLSR (Partial least square regression) and MLR (Multiple linear regression) models were tested to predict CP (Crude protein), NDF (Neutral detergent fiber) and ADF (Acid detergent fiber). Results showed the best model was found at PLSR that had correlation coefficient (R) on prediction and measurement on test set of 0.89, 0.80, and 0.71 for CP, NDF and ADF respectively and with RMSEP (root mean squared error of prediction) value of 1.04, 1.34, and 2.30. For digital image, expert score on each sample was calibrated with LDA (linear discriminant analysis) model and 79 features extracted from image were used. Results indicated that two groups classification accuracy was 100% in color for test set. For smell detection, four features were extracted from the response curve and LDA for two groups was tested. The classification accuracy was about 87% in test set. The study developed a GUI (Graphic user interface) to integrate these three quality evaluations for field application. In terms of contribution, this study developed a hay quality evaluation system that offers quick and nondestructive method. The system is portable and can be carried and operated in field.
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Inhibitory Effect of Danazol on Melanogenesis in Mouse B16 Melanoma Cells
Te-Sheng Chang,Jia-Jhen Lin 대한약학회 2010 Archives of Pharmacal Research Vol.33 No.12
In the present study, more than 200 generic drugs were screened to verify their applicability as a skin-lightening agent using mouse B16 melanoma cells. Of the numerous agents, danazol was found to inhibit melanogenesis in B16 cells in a dose-dependent manner with an IC50 value of 9.3 μM. In addition, danazol reduced cellular tyrosinase activity in B16 cells but did not directly inhibit the murine tyrosinase activity in the cell-free system. Western blotting analysis confirmed that danazol downregulated the levels of tyrosinase protein in B16 cells, and reverse-transcription polymerase chain reaction (RT-PCR) analysis revealed that danazol did not downregulate the levels of tyrosinase mRNA in the cells. These results indicate that danazol inhibits melanogenesis in B16 cells via reducing the tyrosinase activity by post-transcriptional regulation.
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