Multiplex PCR Detection for 3 Events of Genetically Modified Maize, DAS-59122-7, TC6275, and MIR604

Ji-Hye Ahn,Jae-Hwan Kim,Su-Youn Kim,Woo-Young Lee,Sun-Hee Park,Hae-Yeong Kim 한국식품과학회 2008 Food Science and Biotechnology Vol.17 No.3

A multiplex polymerase chain reaction (PCR) method was developed to simultaneously detect 3 events of genetically modified (GM) maize. The event-specific primers were used to discriminate the following 3 events of GM maize (DAS-59122-7, TC6275, and MIR604) using multiplex PCR method. The zein gene was used as an endogenous maize reference gene in the multiplex PCR detection. The primer pair Zein-F/R producing a 99 bp amplicon was used to amplify the zein gene. The primer JI-Das-F1/R1 for DAS-59122-7, JI-TC6275-F3/R3 for TC6275, and JI-MIR F1/R1 for MIR604 yielded an amplicon of 130, 162, and 197 bp, respectively. The detection limit of multiplex PCR was 1% for DAS-59122-7, TC6275, and MIR604 for one reaction.

대학생의 우울정도 : 간호학전공과 타전공대학생을 중심으로

김지연,류나은,이소라,이지희,정윤정,주지연,황인혜 이화여자대학교 간호과학대학 2012 이화간호학회지 Vol.- No.46

Purpose: This study was designed to investigate sample’s general characteristics and compare depression degree among sample’s general characteristics and students’ major(nursing vs. other major). Methods: The subjects consisted of 318 university students. Data was collected by self-reported questionnaires, which were constructed BDI score. Data was analyzed by the SPSS/PC WIN 19.0 program. Results: The depression of nursing students and other major students was not significantly different according to BDI score. Statistically significant difference was identified among sleeping. Conclusion: Specific study focused on the practice time should be done to confirm the depression of nursing major and other majors. Detailed support programs which specifically deal with sleeping should be developed to effectively reduce the harmful effects of individual vulnerability. Therefore, prevention and management system to reduce depression degree for university students is needed.

일반논문 : 디지털 기술시대의 대중예술과 소녀의 복제

한지희 ( Ji Hee Han ) 한국비교문학회 2013 比較文學 Vol.0 No.59

This paper proposes ``cultural engineering`` as a new term for cultural analysis and explores the social meaning of pop-art in the digital age. Based upon Jody Berland`s notion of "cultural technologies," this paper takes note of the digital-based lifestyle that allows contemporary urban laborers to use digital spatial-temporal modes of existence at their commands. By framing such digital transformation through the tools of ``copy,`` ``cut,`` ``past,`` and ``send`` in the context of genetic engineering technologies such as gene-splicing and cloning, it makes attempts to understand the social dimensions surrounding the popular desire for re-making the original and to muse over the social potentials of the new digital culture: whether it will be able to promise the social empowerment for the digital masses as Walter Benjamin responded to the emergence of the cinema in the early twentieth century. As for praxis, this paper applies the new term to the analysis of director Kim Ji-woon`s "Janghwa, Honglyeon" and Charles and Thomas Guard brother`s "The Uninvited: A Tale of Two Sisters and tries to reveal the patriarchal lag that two cinematic editions of a traditional Korean folk tale "Janghwa and Honglyeon" conceal in the process of cloning the sexuality and body of "Sonyeo." In doing so, this paper suggests Korean teen-age girls still remain the specter of the patriarchal texts of girlhood since Janghwa and Honglyeon.

조혈모세포이식 후 발생한 주폐포자층 폐렴에 대한 고찰

주지현,최정현,이동건,백지연,고윤호,이혜정,김세희,신호진,박윤희,박지영,김유진,신완식,김춘추 대한감염학회 2001 감염 Vol.33 No.4

Background : Pneumocytitis cainii pneumonia (PCP) can occur in immunocompromised hosts especially such as AIDS or cancer patients. Although recent research had focused on PCP in AIDS patients, few studies have described the clinical presentations of PCP in recipients of stem cell transplantation (SCT). We evaluated the clinical manifestations of PCP in SCT patients admitted at St. Mary's hospital, Seoul, Korea. Methods : The medical records of 17 PCP patients undergoing SCT between Feb. 1998 and Feb. 2000 were reviewed. The diagnosis of PCP was confirmed through the demonstration of Pneumocytitis cainii via either cytology of brochoalveolar lavage (BAL) or histological technique of lung biopsy. CMV disease and CMV infection were confirmed by BAL culture and antigenemia respectively . Results : Seventeen patients were all recipients of allogeneic SCT and 7 of 17 patients were performed non-sibling SCT. Patients presented with symptoms including brief period (4 ∼23 days) of fever (76%), dyspnea (70%), cough (64%), and signs such as rare(58.8%), Sixteen patients (94%) had been receiving immunosuppressive agent such as cyclosporine A (64%) or Fk506 (35%) without PCP prophylaxis. Eleven patients (64%) were treated with corticosteroid with mean dose of 16 mg/day prednisolone and mean duration of 4.6 months after post-SCT period. Twelve patients were co-infected with CMV. Another co-infected miCroorganisms were Pseudomonas aeruginosa, Mycobacterium tuberculosis, herpes simplex virus, parainfluenza virus, Average duration of treatment with trimethoprim-sulfamethoxazole (TMP/SMX) was 21 ±9 days. Four patients died, and three of them were related with PCP. Conclusion : PCP developed frequently in patients who were taking immunosuppressive drug due to graft versus host disease or were not taking TMP/SMX prophylaxis. High risk patients showing fever, cough, or dyspnea should be considered to take early bronchoscopic intervention for detection of PCP. When treat for PCP, it also be considered to the possibility of coinfection such as CMV. (Korean J Infect Dis 33:273∼279, 2001)

카이토산을 이용한 방사성스트론튬 오염의 치료

김지열,송호천,양광희,최근희,채기문,범희승,김광윤 대한방사선 방어학회 1994 방사선방어학회지 Vol.19 No.3

본 연구에서는 마우스에 이미 오염된 방사성스트론튬(Sr-85)을 제거하는데 수용성카이토산이 어느정도 효과가 있는지 알아보고자 하였다. Sr-85를 정맥주사한 경우 주사후 1일째의 체내 잔류량은 72.9 ±5.7%, 5일째는 57.7 ±1.9%, 7일째는 54.2 ±1.4%로 서서히 감소하였으며, Sr-85를 복강내 주사한 경우는 주사후 5일째 54.4 ±1.2%, 15일째 50.6 ±0.8%로 정맥주사에 비해 낮은 잔류량을 보였다(5일째 잔류량의 비교, P<0.05), 0.3% 수용성카이토산을 1회 정맥주사해준 제21군 및 10% 수용성카이토산을 식이중에 섞어 먹인 제5군은 각각의 대조군에 비해 체내 잔류방사능의 차이가 없었으나 (P>0.05), 0.3% 수용성카이토산을 3일간 정맥주사한 제3군과 3% 수용성카이토산을 이틀 간격으로 15일간 복강내 주사한 제6군에서는 각각의 대조군에 비해 낮은 잔류방사능을 보였다. (P<0.01). 결론적으로 수용성카이토산을 연속적으로 정맥투여하거나 복강내 투여하는 경우에는 스트론튬의 골대사촉진 또는 골중의 스트론튬과의 반응등을 통해 그 배출을 촉진시킬 것으로 사료되었다. The aim of the present study was to elucidate the effect of the water soluble chitosans on the removal of contaminated radiostrontium(Sr-85) from the bone of mice. The remaining radioactivities in intravenously injected controls(group 1) were higher than in intraperitoneally injected controls (group 4, P<0.01). The % retention at day 5 were 57.7 ±1.9%, 54.4 ±1.2%, respectively. Single intravenous injection of 0.3% water soluble chitosan and continuous oral ingestion of 10% water soluble chitosan for 15 days were ineffective on the removal of contaminated radiostrontiums. Multiple intravenous or intraperitoneal injections of water soluble chitosan effectively removed contaminated radiostrontiums (P<0.01 vs controls). In conclusion, water soluble chitosan might remove once incorporated radiostrontium from bones of mice. further studies were needed to elucidate the mechanism of the removal.

수사적 상황과 역할에 따른 대통령 수사학 장르 분석

박성희(Sung Hee Park),이자원(Jawon Lee),석지윤(Ji Yoon Seok) 한국수사학회 2013 수사학 Vol.0 No.19

Rhetorical genre represents a class of messages having important structural and content similarities that creates special expectations among listeners. Presidential rhetoric delivers textual features to the similar effect of creating trust and bonding people. Based on the arguments of Bitzer and Hart, this study attempts to compare and contrast two presidential addresses that were delivered in two different time and spacial settings. For this purpose, former President Park Jung Hee’s 1963 National Press Club address in Washington and President Park Geun Hye’s 2013 address in U.S. Congress in Washington were chosen. As analytic tools, Hart’s credibility strategy index and I-reference style were used, along with Bitzer’s elements in rhetorical situation. The results indicated that two Presidents showed similar patterns in their use of credibility strategy and use of I-reference positioning them in a certain role in their speeches. While two speeches, each of which 48 years apart, revealed different rhetorical situations in terms of their exigences, audiences, and contraints, respective presidents retained similar generic features in their addresses. From the results, it could be discussed that the presidential rhetoric as a generic form has a unique construct of communicating messages that pronounce their unique social role and solidate trust system in society.

문학 장르별 한국 문화교육 연구 -고향과 관련한 작품들을 중심으로-

김지안 ( Kim Ji An ),이정희 ( Lee Jung Hee ) 한국사회복지경영학회 2021 사회복지경영연구 Vol.8 No.2

고향에 대한 한국인의 정서는 문학작품에 녹아들어 대를 이어 향유되고 있다. 한국어학습자들 또한 고향을 떠나 한국사회라는 타향에서의 삶을 살기에 고향에 대한 남다른 의미를 가질 것이다. 따라서 한국어학습자 자신을 비추어 볼 수 있기도 하며, 한국인들이 갖는 남다른 ‘고향’에 대한 정서를 여러 문학 장르의 문학작품을 통해 함께 공유하고 소통하고자 한다. 본 고에서는 한국어교육에 있어서 한국어 학습자에게 읽기 텍스트로서의 접근성과 언어 수준을 고려한 문장의 길이 그리고 내용 파악의 용이성을 염두에 두고 고향이라는 주제와 소재가 포함된 문화요소를 고려하여 단편소설, 수필, 현대시, 시조의 장르를 선정하였다. 소설에서는 고향 상실과 고향 회귀에 대해 심도 있게 다룬 황석영의 ‘삼포로 가는 길’과 문순태의 ‘징 소리’를 선택하였다. 외지를 떠도는 주인공들에게 고향은 마음속에 담겨 있고, 돌아가고 싶은 곳으로 정착하고픈 곳이다. 수필에서는 수필가로 인지도가 있고, 내 고향에서 고향에 대한 애착을 잘 표현한 치옹 윤오영의 ‘내 고향’과 우리나라의 대표적인 국어학자로서 국어사연구 및 국어정책 발전에 힘썼으며, 월남 학자로서 고향에 대한 의식이 남달랐고, 고향과 어머니를 그리워하는 마음도 강하게 나타난 해암 김형규의 ‘고향’을 중심으로 논의하였다. ‘내 고향’에서는 고향에 대한 애착과 자부심이 ‘고향’에서는 두고 온 고향과 홀로 계신 어머니를 생각하며 회한에 빠진 모습이 잘 나타난다. 시에서는 정지용의 ‘향수’와 이시영의 ‘마음의 고향6 초설’을 선택하였다. ‘향수’에서는 고향의 정경을 잘 묘사하고 있고, 감각적 이미지를 사용하여 고향에 대한 그리움을 나타내고 있고, ‘내 마음의 고향’에서는 고향을 잃은 상실감과 그리움의 정서를 반어적으로 강조하고 있다. 시조에서는 고향에 대한 작품을 많이 남긴 백수 정완영의 ‘고향생각’과 초정 김상옥의 ‘사향’을 선택하였다. ‘고향생각’에서는 단지 태어나 자란 곳이 아닌 조상 곁으로 돌아가는 공간으로의 고향으로 그려지고 있다. ‘사향’에서는 고향을 떠나 일경의 추적을 피하면서도 고향에 대한 애틋한 생각을 담고 있다. 작품들에서 고향은 한국인들에게 고향은 자기가 태어난 곳을 넘어 자신의 뿌리가 되고 힘들 때 생각나고, 언젠가는 돌아가야 할 곳으로 생각하고 있다. 문학 장르별 문화 교육 방안으로 소설과 수필은 고향을 주제로 써보는 것을, 시와 시조에서는 모방시 짓기와 시조 창작 활용을 제시하였다. 고향이란 테마에 한국인의 효와 가족 사랑을 묶는다면 한국어 문화교육 현장에서 좋은 소재가 될 것이고, 한국의 문화를 교육하는데 일조할 것이다. Koreans' emotions about their hometown have been melted into literary works and enjoyed for generations. Korean language learners will also have a special meaning for their hometowns because they leave their hometowns and live in other regions of Korean society. Therefore, Korean learners themselves can be seen in light of themselves, and they intend to share and communicate with Koreans through literary works of various literary genres. In this paper, short stories, essays, modern poems, and sijo genres were selected in consideration of cultural elements including the theme and subject of hometown, with Korean learners in mind of accessibility as reading texts, language level, and ease of identification. In the novel, Hwang Seok-young's "The Way to Sampo" and Moon Soon-tae's "Jingsori," which dealt with in-depth loss of hometown and return to hometown, were selected. For the protagonists wandering around the country, their hometown is contained in their hearts and they want to settle down as a place they want to return to. In his essay, he was recognized as an essayist, and he worked hard to study Korean history and develop Korean language policies as a representative Korean scholar in Korea, and discussed Haeam Kim Hyung-gyu's hometown, which showed a strong sense of longing for his hometown and mother. In My Hometown, attachment and pride to my hometown are well seen in regret, thinking about my hometown and my mother who is alone. The poem chose Jeong Ji-yong's "Nostalgia" and Lee Si-young's "Hometown of Heart 6." In "Nostalgia," the scenery of the hometown is well described, the longing for the hometown is expressed using a sensuous image, and in "Hometown of My Heart," the emotion of loss and longing for the loss of hometown is ironically emphasized. In the progenitor's book, Baeksu Jeong Wan-young's "Hometown Thoughts" and Chojeong Kim Sang-ok's "Sahyang" were chosen. In "Hometown Thinking," it is depicted as a hometown that goes back to the ancestor's side, not just a place where he was born and raised. ‘Sahyang’ contains affectionate thoughts about the hometown while avoiding the pursuit of Ilgyeong, leaving his hometown. In the works, I think of my hometown as a place for Koreans to go beyond where I was born, become my roots, think of when I have a hard time, and go back someday. As a cultural education plan for each literary genre, novels and essays were written on the theme of hometown, and poems and sijos suggested writing imitations and the use of sijo creation. If Koreans' filial piety and family love are tied to the theme of hometown, it will be a good material in the Korean language culture education field and help educate Korean culture.

임신마우스에서 수용성카이토산에 의한 태아의 방사성스트론튬 오염 억제

채기문,최근희,양광희,범희승,김지열,김광윤 대한방사선 방어학회 1994 방사선방어학회지 Vol.19 No.3

핵분열생성물중의 하나인 방사성스트론튬은 임신한 쥐에서 태반을 빠르게 통과하여 태아를 오염시킨다. 독성이 적은 천연착화제로서 마우스체내의 방사성스트론튬(Sr-85)의 제거에 효과적인 것으로 알려진 카이토산을 이용하여 Sr-85의 태반오염억제에 관한 연구를 하였다. 실험군은 일반식이를 공급한 대조군과 임신 17일째에 수용성카이토산을 피하(1% 카이토산), 구강(10% 카이토산), 복강(0.3% 카이토산)으로 주사한 카이토산군으로 분류하였다. 피하주사군은 Sr-85 오염후 카이토산을 주사한 군이며 복강과 구강주사군은 Sr-85 오염젼 15일간 카이토산을 공급한 군이었다. 출산과 동시에 어린마우스의 전신을 측정한 겨러과 출생후 7일째에 대조군이 2.8 ±0.3%의 전신축적율을 나타낸 반면 피하, 구강, 복강, 주사군은 각각 1.2 ±0.1%, 1.4 ±0.1%, 1.6 ±0.2%로 나타났다. 결과적으로 임신한 마우스에서 수용성카이토산은 방사성스트론튬의 태반오염을 유의하게 억제하는 것으로 사료된다. Radiostrontium passes the placental barrier in pregnant rodents very well. Chitosan, a natural nontoxic chelator, was reported to reduce whole body retention of radiostrontium in mice. The aim of the present study was to evaluate water soluble chitosan as a blocking agent of transplacental transfer of radiostrontium in pregnant mice. Twenty pregnant mice wre divided into four groups : control and three groups of chitosan treatment(groups 1 to 3). Sr-85(15KBq in 0.2ml saline) was subcutaneously injected into pregnant mice at the 17th day of pregnancy. In control mice, 0.2ml saline was given 5 hours after the injection of Sr-85. In group 1.1% water soluble chitosan was given subcutanously for two days, twice daily after the injection of Sr-85. In group 2, 10% water soluble chitosan was given orally for 15 days before conception. In group 3, 0.3% water soluble chitosan was injected intravenously for 15 days, once daily before conception. Gamma counting of newborns were done at days 0, 2 and 7 after their births. Whole body retention of Sr-85 in newborns of control mice at days 0, 2, 7 were 3.1 ±0.3%, 2.9 ±0.3%, 2.8 ±0.3% respectively. In experimental groups, whole body retention of Sr-85 was significantly lower thanthat of control(p<0.01) and no statistical difference was noted between them. In group 1, the values were 2.1 ±0.3%, 1.4 ±0.1%, 1.4 ±0.1%, respectively. In group 3, they were 2.1 ± 0.2%, 1.7 ± 0.2%, 1.6 ± 0.2%, respectively. In conclusion, the water soluble chitosan reduced transplacental contamination of radiostrontium in pregnant mice.

진단 기준에 따른 취학전 어린이의 중증 유아기 우식증 유병률 비교

임경욱,이광희,라지영,이동진,안소연,김지영,송지현,김윤희 大韓小兒齒科學會 2008 大韓小兒齒科學會誌 Vol.35 No.4

연구목적은 중증 유아기 우식증에 대한 두 개의 정의를 진단 기준으로 하였을 때 각 기준에 따른 유병률이 일치하는 정도를알기 위함이었다. 연구대상은 익산시 어린이집 중에서 무작위로 추출한 7개 어린이집의 36개월 이상 71개월 이하 어린이401명 이었다. 치경과 탐침을 사용한 구강검사를 통하여 치아별 우식경험유치면 (dmfs)의 수를 조사하였으며 . 중증 유아기 우식증의 진단 기준은 (1) 상악 전치에 1개 이상의, 와동이 형성된 우식,상실(우식증에 의한), 충전 평활면이 있거나 (2) 우식,상실,충전 치면의 수가 3세는 4개 이상.4세는 5개 이상.5세는 6개 이상인 경우로 하였다. 상악 전치부 우식증에 의한 우식유병률과 우식경험유치면수(dmfs)에 의한 우식유병률은 3세에서 각각 26.7%. 28.9%, 4세에서 각각 32.4%, 30.4%, 5세에서 각각 39.4%, 41.3%, 전체에서 각각 35.4%, 35.9%으로서. 연령별로는 약간의 차이가 있었으나 전체에서는 차이가0.5%로서 거의 일치하였다. 따라서 중증 유아기 우식증의 진단은 두 가지 기준 중에서 어느 하나를 사용하더라도 비슷한 결과가 나을 것이라는 것을 확인할 수 있었다. The purpose of study was to compare the prevalences of severe early childhood cahes(S-ECC) using two different diagnostic criteria. The subjects of this study included 401 preschool children from 36 to 71 months of the randomly selected seven nurseries in Iksan city. Severe early childhood caries was defined as (1) 1 or more cavitated, missing (due to caries), or filled smooth surfaces in primary maxillary anterior teeth or (2) a decayed, missing, or filled score of ≥4 (age 3), ≥5 (age 4), or ≥6 (age 5) surfaces. The prevalence determined by maxillary anterior caries and the prevalence determined by dmfs were 26.7%, 28.9% in 3 years, 32.4%, 30.4% in 4years. 39.4%, 41.3% in 5 years, and 35.4%, 35.9% in all, respectively. The results suggest that the two diag-nostic criteria of the severe early childhood caries yield almost the same results.

노인의 영적 간호요구

최미혜,김경희,김귀옥,김기숙,김수강,김정신,김춘숙,노흥진,박지연,성혜연,오명선,이선희,이원옥,이윤영,이현수,장명재,차혜경,채정선,홍상희 중앙대학교 의과대학 간호학과 간호과학연구소 2001 중앙간호논문집 Vol.5 No.1

This study was designed to exam the aged's needs for spiritual nursing care. The purpose was to serve as a basis for the development of spiritual nursing practice. The major findings are as follows : 1. The degree of needs for spiritual nursing care as area was that needs of love and relationship mean 22.0, needs of meaning and object mean 28.2, needs of forgiving mean 13.5. Total needs for spiritual nursing care mean 63.7, which was on the upper middle level. The needs of meaning and object was rated highest. 2. Among the general characteristics of the subjects, needs of love and relatiohship wasn't significanlty different. 3. Among the general characteristics of the subjects, needs of meaning and object was significantly different according to two factors : age(F=7.260, p=0.001), religion(F=5.275, p=0.001). Higher needs of meaning and object was possessed by the older than the younger, by the one who have religion than the other. 4. Among the general characteristics of the subjects, four factors made a significantly difference to needs of forgiving : sex(t=-2.851, p=0.006), age(F=8.201, p=0.001), religion(F=6.928, p=0.000), disease(t=2.327, p=0.024). Higher needs of forgiving was possessed by man than woman, by the older than the younger, by the one who have religion than the other, by the one who have disease than the other.