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Zhi-Rong Zhong,Zhi-rong Zhang,Ji Liu,Yong Deng,Hong-wei Zhang,Yao Fu,Qing-guo Song,Qin He 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.1
A novel non-viral gene delivery system, Procationic-Liposome-Protamine-DNA complexes (PLPD) which could further adsorb transferrin on the surface as a targeting ligand to form Tf- PLPD, was prepared and characterized before and after lyophilization. The size distribution of Tf-PLPD was in the range of 240 ± 12 nm and the zeta potential was -24.10 ± 2.5 mV. The transfection efficiencies of PLPD and Tf-PLPD were 12.18 ± 3.8 and 24.26 ± 2.6 mU β-galactosidase/ mg protein respectively. The lyophilization and the presence of serum didn’t affect the tansfectivities of PLPD or Tf-PLPD. Compared to LipofectamineTM 2000 (Invitrogen, U.S.A.), the procationic liposomes had less cytotoxicity to cells. In summary the procationic lipoplex described here, combining the condensing effect of protamine and the targeting capability of Tf, was a perspective non-viral vector for gene delivery system.
Ryu, Ji-Kan,Zhang, Lu Wei,Jin, Hai-Rong,Piao, Shuguang,Choi, Min Ji,Tuvshintur, Buyankhuu,Tumurbaatar, Munkhbayar,Shin, Sun Hwa,Han, Jee-Young,Kim, Woo Jean,Suh, Jun-Kyu Blackwell Pub 2009 The journal of sexual medicine Vol.6 No.7
<P>Endothelial cell-to-cell junctions are crucial for vascular formation, networking, and remodeling of blood vessels as well as for inducing and integrating intracellular signals.</P>
Huang, Rong-Hui,Lu, Ri-Yu,Chen, Wen,Chen, Ji-Rong Korean Meteorological Society 2003 大氣 Vol.13 No.2
Recent advances in the studies on the interaction between Asian monsoon and ENSO cycle are reviewed in this paper. Through the recent studies, the East Asian summer monsoon circulation system and the East Asian climate system have proposed. Moreover, different responses of the (winter and summer) monsoon circulation and summer rainfall anomalies in East Asia to ENSO cycle during its different stages have been understood further. Recently, the studies on the dynamical effect of East Asian monsoon on the thermal variability of the tropical western Pacific and ENSO cycle have been greatly advanced. These studies demonstrated further that ENSO cycle originates from the tropical western Pacific, and pointed out that the dynamical effect of East Asian winter and summer monsoons on ENSO cycle may be through the atmospheric circulation and zonal wind anomalies over the tropical western Pacific, which can excite the oceanic Kelvin wave and Rossby waves in the equatorial Pacific. Besides, the scientific problems in the interaction between Asian monsoon and ENSO cycle, which should be studied further in the near future, are also pointed out in this paper.
Ryu, Ji-Kan,Tumurbaatar, Munkhbayar,Jin, Hai-Rong,Kim, Woo Jean,Kwon, Mi-Hye,Piao, Shuguang,Choi, Min Ji,Yin, Guo Nan,Song, Kang-Moon,Kang, Yong-Jin,Koh, Young Jun,Koh, Gou Young,Suh, Jun-Kyu Blackwell Pub 2012 JOURNAL OF SEXUAL MEDICINE Vol.9 No.12
<P>Men with diabetic erectile dysfunction (ED) often have severe endothelial dysfunction and respond poorly to oral phosphodiesterase-5 inhibitors.</P>
레드비트를 함유하는 화장품의 담배 연기에 의한 피부 지질 산화 방지 효과
서초롱 ( Cho Rong Seo ),하태현 ( Tae Hyun Ha ),문지영 ( Ji Young Moon ),김정미 ( Jeong Mi Kim ),박병권 ( Byoung Kwon Park ),이지원 ( Ji Won Lee ),박진오 ( Jin Oh Park ),신진희 ( Jin Hee Shin ) 대한화장품학회 2018 대한화장품학회지 Vol.44 No.2
최근 화장품 시장에서 안티폴루션 효능이 있는 화장품은 새로운 피부 건강을 위한 해결책으로 나타나고 있다. 외부(대기) 오염물질에 의해 피부의 산화 기전을 매개하는 주요 원인 인자들로 오존, UV, 미세먼지 및 담배연기 등을 꼽을 수 있다. 담배 연기의 노출은 직·간접적으로 피부 표피 내 지질의 산화를 야기한다. 피부의 지질 산화에 의해 squalene과 squalene monohydroperoxide의 비율 변화가 발생하고, malondialdehyde (MDA)가 지질산화 산물로 생성된다. 따라서, 본 연구에서는 담배연기 노출 시 발생하는 피부 산화에 대하여 레드비트를 함유하는 화장품이 방지할 수 있는 효능이 있는지에 대하여 MDA의 생성량으로 관찰하였다. 시험대 상자 전박부를 세 영역(음성대조, 양성대조, 시험제품)으로 나누어 각 지름 3.3 cm의 원으로 구획하고, 15분간 담배연기에 노출 후 피부 표면으로부터 지질막을 걷어낸 후 TBARS assay를 통하여 MDA를 정량 하였다. 음성대조(무도포, 미노출)에 비해 양성대조(무도포, 담배연기 노출)의 MDA 생성량이 3.7배 증가된 결과로, 오염물질인 담배연기에 의한 피부의 산화적 손상을 확인하였다. 반면에 레드비트를 함유하는 화장품을 미리 도포한 영역은 양성대조에 비해 MDA 생성량이 25% 감소하는 결과를 나타내었다. 결론적으로, 담배연기 노출은 피부표피 내 지질 산화를 야기하며, 레드비트를 함유하는 화장품이 이러한 대기환경오염으로부터 방어적 효과(안티폴루션 효과)를 보이는 것을 확인하였다. In cosmetics market, anti-pollution products recently come up with new solution for skin health. Environmental oxidation mechanisms are realized as bio-marker of atmospheric pollution upon skin by environmental pollutant such as ozone, UV rays, particulate matter (PM) as well as cigarette smoke. The exposure of cigarette smoke directly or indirectly causes the oxidation of the stratum corneum skin lipids, resulting in the conversion of squalene to squalene monohydroperoxide and/or generation of malondialdehyde (MDA) as a product of lipid peroxidation. The aim of this study is to see whether new cosmetics product containing red beet has anti-oxidation effect on skin exposed by cigarette smoke. So as to determine oxidative damage to human skin at biochemical level, each unit area of volar forearms was exposed to cigarette smoke through device (3.3 cm, diameter) for fifteen minutes, then measured MDA using standardized TBARS assay kit. Compared to negative control (untreated and unexposed area), the level of MDA was significantly increased at positive control (untreated and exposed area) more than 3.7 times, indicating the pollutant induced-oxidative damage on the skin barrier. Whereas, the pre-applied area with the cosmetics products containing red beet revealed a decrease of 25% compared with positive control. As reports, these data demonstrated that cigarette smoke induce peroxidation of stratum corneum skin lipids. Conclusively, we suggest that anti-pollution effect of the cosmetics product containing red beet is beneficial to prevent the oxidation of skin lipids by atmospheric pollution.
Jin, Hai-Rong,Kim, Woo Jean,Song, Jae Sook,Piao, Shuguang,Choi, Min Ji,Tumurbaatar, Munkhbayar,Shin, Sun Hwa,Yin, Guo Nan,Koh, Gou Young,Ryu, Ji-Kan,Suh, Jun-Kyu American Diabetes Association 2011 Diabetes Vol.60 No.3
<P><B>OBJECTIVE</B></P><P>Patients with diabetic erectile dysfunction often have severe endothelial dysfunction and respond poorly to oral phosphodiesterase-5 inhibitors. We examined the effectiveness of the potent angiopoietin-1 (Ang1) variant, cartilage oligomeric matrix protein (COMP)-Ang1, in promoting cavernous endothelial regeneration and restoring erectile function in diabetic animals.</P><P><B>RESEARCH DESIGN AND METHODS</B></P><P>Four groups of mice were used: controls; streptozotocin (STZ)-induced diabetic mice; STZ-induced diabetic mice treated with repeated intracavernous injections of PBS; and STZ-induced diabetic mice treated with COMP-Ang1 protein (days −3 and 0). Two and 4 weeks after treatment, we measured erectile function by electrical stimulation of the cavernous nerve. The penis was harvested for histologic examinations, Western blot analysis, and cGMP quantification. We also performed a vascular permeability test.</P><P><B>RESULTS</B></P><P>Local delivery of the COMP-Ang1 protein significantly increased cavernous endothelial proliferation, endothelial nitric oxide (NO) synthase (NOS) phosphorylation, and cGMP expression compared with that in the untreated or PBS-treated STZ-induced diabetic group. The changes in the group that received COMP-Ang1 restored erectile function up to 4 weeks after treatment. Endothelial protective effects, such as marked decreases in the expression of p47<SUP>phox</SUP> and inducible NOS, in the generation of superoxide anion and nitrotyrosine, and in the number of apoptotic cells in the corpus cavernosum tissue, were noted in COMP-Ang1–treated STZ-induced diabetic mice. An intracavernous injection of COMP-Ang1 completely restored endothelial cell-cell junction proteins and decreased cavernous endothelial permeability. COMP-Ang1–induced promotion of cavernous angiogenesis and erectile function was abolished by the NOS inhibitor, <I>N</I>-nitro-L-arginine methyl ester, but not by the NADPH oxidase inhibitor, apocynin.</P><P><B>CONCLUSIONS</B></P><P>These findings support the concept of cavernous endothelial regeneration by use of the recombinant Ang1 protein as a curative therapy for diabetic erectile dysfunction.</P>
Src as a Therapeutic Target in Biliary Tract Cancer
Nam, Ah-Rong,Kim, Ji-Won,Park, Ji Eun,Bang, Ju-Hee,Jin, Mei Hua,Lee, Kyung-Hun,Kim, Tae-Yong,Han, Sae-Won,Im, Seock-Ah,Kim, Tae-You,Oh, Do-Youn,Bang, Yung-Jue American Association for Cancer Research 2016 Molecular Cancer Therapeutics Vol.15 No.7
<P>Src, a nonreceptor tyrosine kinase, is involved in a number of cancer-related signaling pathways and aberrantly activated in biliary tract cancer (BTC). This study aimed to elucidate the potential role of Src as a therapeutic target in BTC. We tested bosutinib, an orally active c-Src/Abl kinase inhibitor, alone or in combination with cytotoxic agents using 9 human BTC cell lines: SNU-245, SNU-308, SNU-478, SNU-869, SNU-1079, SNU-1196, HuCCT1, TFK-1, and EGI-1. Of these, SNU-308 and SNU-478 were relatively sensitive to bosutinib. Bosutinib abrogated phosphorylation of Src and its downstream molecules, and significantly increased G(1) cell-cycle arrest and apoptosis. Bosutinib significantly inhibited cell migration and invasion and decreased epithelial-mesenchymal transition markers. Bosutinib combined with gemcitabine or cisplatin showed synergistic antiproliferative and antimigratory effects. In addition, this combination further inhibited phosphorylation of Src and its downstream molecules and decreased epithelial-mesenchymal transition marker expression compared with bosutinib alone. We established a SNU-478 xenograft model for in vivo experiments, because SNU-478 was more tumorigenic than SNU-308. Bosutinib combined with gemcitabine or cisplatin showed significantly more potent antitumor effects than bosutinib alone. Bosutinib combined with gemcitabine further decreased Ki-67 expression and Src phosphorylation, and further increased TUNEL expression. Our data suggest that Src might be a potential therapeutic target in BTC. Bosutinib demonstrated promising antitumor activity alone or in combination with gemcitabine or cisplatin in BTC cells, which supports further clinical development in patients with advanced BTC. (C) 2016 AACR.</P>