Synthesis of Thermoresponsive Hyperbranched Polyamidoamine and the Molecular Weight, pH, and Anion Sensitive Thermoresponsive Properties Thereof

Yi Liu,Yu Chen,Xun-Yong Liu,Hua-Ji Liu,Fa Cheng 한국고분자학회 2012 Macromolecular Research Vol.20 No.6

Hyperbranched polyamidoamine (HPAMAM) polymers, chemically analogous to the commercially available PAMAM dendrimer, were modified with isobutyric anhydride to result in isobutyramide (IBAm) terminated HPAMAMs (HPAMAM-IBAm). The aqueous solutions of HPAMAM-IBAm polymers had the lower critical solution temperature (LCST). The lower molecular-weight HPAMAM-IBAm exhibited higher LCST and the LCST difference was around 18 oC for one pseudo-generation variation. Further, the hyperbranched thermoresponsive polymers exhibited much lower LCSTs than the corresponding dendrimers with similar molecular weight. The LCST of HPAMAM-IBAm was pH sensitive. At pH below 10, the LCST increased significantly upon decreasing the pH,whereas, at pH above 10, the LCST decreased slowly with an increasing pH value. Nine sodium salts were used to measure the anion effect on the LCST of HPAMAM-IBAm. It was found that the LCST could also be modulated up or down in a broad range by simply adding a small amount of different kinds of inorganic anions. The specific ranking of inorganic anions in salting-out HPAMAM4-IBAm polymer was in accordance with the well-known Hofmeister series.

Ab Initio Study of Mechanism of Forming Spiro-Heterocyclic Ring Compound Involving Si and Ge from Dichlorosilylene Germylidene (Cl₂Si-Ge:) and Acetone

Liu, Dongting,Ji, Hua,Lu, Xiuhui Korean Chemical Society 2012 Bulletin of the Korean Chemical Society Vol.33 No.12

The mechanism of the cycloaddition reaction between singlet state dichlorosilylene germylidene ($Cl_2Si=Ge:$) and acetone has been investigated with B3LYP/6-$31G^*$ and B3LYP/6-$31G^{**}$ method, from the potential energy profile, we predict that the reaction has one dominant reaction pathway. The presented rule of the reaction is that the two reactants firstly form a Si-heterocyclic four-membered ring germylene through the [2+2] cycloaddition reaction. Because of the 4p unoccupied orbital of Ge atom in the Si-heterocyclic four-membered ring germylene and the ${\pi}$ orbital of acetone forming a ${\pi}{\rightarrow}p$ donor-acceptor bond, the Si-heterocyclic four-membered ring germylene further combines with acetone to form an intermediate. Because the Ge atom in the intermediate hybridizes to an $sp^3$ hybrid orbital after the transition state, then, the intermediate isomerizes to spiro-heterocyclic ring compound involving Si and Ge (P4) via a transition state.

Monosized core-shell Fe3O4(Fe)/Au multifunctional nanocrystals.

Liu, Hong-Ling,Wu, Jun-Hua,Min, Ji Hyun,Lee, Ju Hun,Kim, Young Keun American Scientific Publishers 2009 Journal of nanoscience and nanotechnology Vol.9 No.2

<P>We have synthesized highly crystalline, monosized Fe3O4(Fe)/Au magnetic-optic multifunctional nanocrystals using a modified polyol process. The formation of the core-shell structure was achieved in two consecutive steps, seeding the Fe3O4(Fe) core followed by coating the gold shell. The characterization demonstrates the core-shell nanostructure and provides particle size and distribution of the nanocrystals. The spectroscopic analysis gives three Fe oxidation states (Fe0, Fe+2 and Fe+3) in the core and the surface plasmon characteristic of the Au nanoshell, while the magnetic measurements show excellent susceptibility of the nanocrystals.</P>

Molecular cloning, chromosomal localization and expression profiling of porcine selenoprotein M gene

Ji-Chang Zhou,Hua Zhao,Jia-Yong Tang,Jun-Gang Li,Xiao-Li Liu,Yu-Mei Zhu 한국유전학회 2011 Genes & Genomics Vol.33 No.5

Selenoprotein M may regulate a myriad of biological processes through its redox function. In pigs, neither the nucleotide sequence nor the amino acid sequence is known. Furthermore,patterns of tissue expression and regulation by dietary selenium (Se) have not been examined. We determined the full coding sequence (CDS) and the chromosomal location of the porcine gene, SELM, and described its expression profile in vivo under different dietary Se concentrations. The cDNA sequence of porcine SELM from the start codon to the poly(A) tail was cloned by reverse transcription PCR. The CDS contained 429bases with a typical mammalian selenocysteine insertion sequence of form 2 (F2) located in the 3′-untranslated region. The gene was mapped to chromosome 14q21, where porcine SELM and its neighboring genes exhibited a similar organization to human homologues on chromosome 22q12.2. The expression pattern of SELM mRNA in muscle, thyroid, cerebral cortex, pituitary, testis, liver, and kidney was analyzed with real-time quantitative PCR in young male pigs fed a Se-deficient corn-soybean meal basal diet supplemented with 0.0, 0.3,or 3.0 mg Se/kg in the form of Se-rich yeast. Though the SELM mRNA abundance in each of the 7 tissues was not affected by the dietary Se concentrations, it was significantly higher in thyroid (P < 0.01) than in cerebral cortex, pituitary,testis, liver, and kidney at all of the 3 dietary Se concentrations.

Associations of TNF-RII rs1061622 With Post-Traumatic Stress Disorder and Their Interplays on Serum Lipids Levels in Adolescents

Ji Cheng Zhang,Jin Hua Wang,Jun Yi Liu,Qi Wei Guo,Jia Lin,Yi Lin Shen,Ke Xin Jia,Jia Jing Cai,Guo Ming Su,Ding Zhi Fang 대한신경정신의학회 2023 PSYCHIATRY INVESTIGATION Vol.20 No.11

Objective To verify effects of rs1061622 at tumor necrosis factor-α receptor II (TNF-RII) gene (<i>TNF-RII</i>) on post-traumatic stress disorder (PTSD) and its interactive effects with PTSD on serum lipids levels in adolescents.Methods PTSD was measured by PTSD Checklist-Civilian Version (PCL-C) in 699 adolescent survivors at 6 months after Wenchuan earthquake in China. A polymerase chain reaction and restriction fragment length polymorphism assay were utilized for <i>TNF-RII</i> rs1061622 genotyping followed by verification using DNA sequencing. Serum triglycerides (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol were tested using routine methods.Results G (deoxyguanine) allele carriers had higher PCL-C scores than TT (deoxythymidine) homozygotes in female subjects. Female adolescents had higher PCL-C scores than male subjects in TT homozygotes. Predictors of PTSD prevalence and severity were different between G allele carriers and TT homozygotes. Subjects with PTSD had lower TG, TG/HDL-C, TC/HDL-C, and higher HDL-C than adolescents without PTSD in male G allele carriers. G allele carriers had higher TG/HDL-C and TC/HDL-C than TT homozygotes in male adolescents without PTSD, and lower TG and TG/HDL-C in male PTSD patients. G allele carriers had higher TG than TT homozygotes only in female adolescents without PTSD.Conclusion These results suggest reciprocal actions of <i>TNF-RII</i> rs1061622 with other factors on PTSD severity, interplays of <i>TNF-RII</i> rs1061622 with PTSD on serum lipid levels, and novel treatment strategies for PTSD and comorbidities of PTSD with hyperlipidemia among adolescents with different genetic backgrounds of <i>TNF-RII</i> rs1061622 after experiencing traumatic events.

The PR10 gene family is highly expressed in Lilium regale Wilson during Fusarium oxysporum f. sp. lilii infection

Hua He,Diqiu Liu,Nannan Zhang,Wei Zheng,Qing Han,Bo Ji,Feng Ge,Chaoyin Chen 한국유전학회 2014 Genes & Genomics Vol.36 No.4

Pathogenesis-related (PR) proteins play keyroles in plant responses to pathogens and abiotic stresses. In this study, nine novel PR genes were isolated from Liliumregale Wilson, which is a wild lily species of Chinawith high-level resistance to the soilborne fungal pathogenFusarium oxysporum f. sp. lilii, and homology analysisclassified them into the PR10 family. These novel LrPR10swere clustered together with PR10s from monocotyledonsin a phylogenetic tree, moreover, phylogenetic analysisdivided the nine LrPR10s into two groups. The main-chainconformation and folding patterns of the LrPR10s werehighly conserved with other plant PR10s. The expressionpatterns of the nine LrPR10s in L. regale during normaldevelopment were examined by QRT-PCR, and the transcriptionlevels of the LrPR10s were relatively high inroots. Furthermore, QRT-PCR analysis indicated that theexpression levels of LrPR10-1, LrPR10-2, LrPR10-5,LrPR10-6, and LrPR10-7 in L. regale roots were up-regulatedby two or more stress-related signaling moleculesincluding salicylic acid, jasmonic acid, ethylene, and H2O2,while the other four LrPR10s were repressed by these foursignaling molecules. In addition, five members of theLrPR10 gene family including LrPR10-2, LrPR10-4,LrPR10-5, LrPR10-6, LrPR10-7, and LrPR10-9 werestrongly induced by F. oxysporum in resistant L. regalecompared with the susceptible Lilium Oriental hybrid‘Siberia’. The other four LrPR10s were down-regulated byF. oxysporum infection. In summary, our results indicatethat the members of PR10 gene family are involved inL. regale defense responses against F. oxysporum f. sp. lilii.

Fabrication of Multifunctional Au Doped CoPt Nanowires

Ji Hyun Min,Hong-Ling Liu,Ju Hun Lee,Jun-Hua Wu,Jae-Seon Ju,Young Keun Kim IEEE 2009 IEEE transactions on magnetics Vol.45 No.6

<P>Au-doped CoPt nanowires were prepared in anodized aluminum oxide (AAO) nanotemplates by electrodeposition and the doping effects on their microstructure and magnetic properties were investigated. Dependent on the deposition conditions, the CoPtAu nanowires can have the cubic or tetragonal phase. The magnetic measurements indicate that the nanowires demonstrate ferromagnetic behavior, showing the easy axis along the direction of the nanowires and strong enhancement in coercivity and squareness prior to annealing, whereas the optical measurements reveal plasmon resonances characteristic of nanostructured Au.</P>

Electrochemical Preparation of Co₃Pt Nanowires

Ji Hyun Min,Jun-Hua Wu,Ji Ung Cho,Ju Hun Lee,Young-Dong Ko,Seung Pil Ko,Hong-Ling Liu,Jin-Seok Chung,Young Keun Kim 한국자기학회 2007 한국자기학회 학술연구발표회 논문개요집 Vol.- No.-

Functions of Membrane-bound Alcohol Dehydrogenase and Aldehyde Dehydrogenase in the Bio-oxidation of Alcohols in Gluconobacter oxydans DSM 2003

Liu-Jing Wei,Ji-lai Zhou,Dan-ni Zhu,Bai-yi Cai,Jin-Ping Lin,Qiang Hua,Dong-Zhi Wei 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.6

In this study a new insight was provided to understand the functions of membrane-bound alcohol dehydrogenase (mADH) and aldehyde dehydrogenase (mALDH) in the bio-oxidation of primary alcohols, diols and poly alcohols using the resting cells of Gluconobacter oxydans DSM 2003 and its mutant strains as catalyst. The results demonstrated that though both mADH and mALDH participated in most of the oxidation of alcohols to their corresponding acid, the exact roles of these enzymes in each reaction might be different. For example,mADH played a key role in the oxidation of diols to its corresponding organic acid in G. oxydans, but it was dispensable when the primary alcohols were used as substrates. In contrast to mADH, mALDH appears to play a relatively minor role in organic acid-producing reactions because of the possible presence of other isoenzymes. Aldehydes were, however, found to be accumulated in the mALDH-deficient strain during the oxidation of alcohols.

MRAS Genetic Variation is Associated with Atherothrombotic Stroke in the Han Chinese Population

Hua Liu,Xiao Qin Huang,Min Yang,Xun Ming Ji,Xin Du,Jian Zheng 대한신경과학회 2013 Journal of Clinical Neurology Vol.9 No.4

Background and Purpose Atherothrombotic cerebral infarction [atherothrombotic stroke (ATS)] shares common risk factors and pathophysiological mechanisms with coronary artery disease (CAD), and both diseases appear to have common susceptibility loci. The muscle RAS oncogene homolog gene (MRAS) has been identified as a susceptibility locus for CAD and is implicated in atherosclerosis. The aim of this study was to elucidate whether the single-nucleotide polymorphisms (SNPs) and haplotypes of MRAS are associated with increased risk of ATS in a population of Han Chinese. Methods A case-controlled association study was conducted in which only patients with ATS (identified as a major subtype in the Korean modification of the Trial of Org 10172 in Acute Stroke Treatment classification) were enrolled. Subgroup analyses were carried out to determine whether the effect of the MRAS polymorphism was specific to age and gender among the subjects. Results In total, 194 ATS and 186 control subjects were included in the present study. Two tagging SNPs were identified in MRAS (rs40593 and rs3755751). A multivariate regression analysis revealed a positive association between rs40593 and ATS under dominant and additive models after adjustment for covariates. Subgroup analyses revealed that there were no gender differences with respect to allele or genotype frequencies between the groups. The AG genotype for rs40593(p=0.028), the CT genotype for rs3755751 (p=0.036), and G-allele carriers (AG plus GG) for rs40593 (p=0.015) exhibited a significant protective effect among those aged ≥45 years. For the haplotype analysis, ATS subjects aged ≥45 years had a higher frequency of the ACAC haplotype (76.0%) than the controls (68.1%; p<0.05); that haplotype was associated with an increased risk of ATS. Conclusions The obtained data suggest a positive association between MRAS and ATS among the Han Chinese. Further studies should be performed with larger sample and among different ethnic populations, and gene-gene or gene-environment interactions should be considered.