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De, Umasankar,Kundu, Soma,Patra, Nabanita,Ahn, Mee Young,Ahn, Ji Hae,Son, Ji Yeon,Yoon, Jung Hyun,Moon, Hyung Ryoung,Lee, Byung Mu,Kim, Hyung Sik The Korean Society of Applied Pharmacology 2015 Biomolecules & Therapeutics(구 응용약물학회지) Vol.23 No.5
Histone deacetylase (HDAC) inhibitors are considered novel agents for cancer chemotherapy. We previously investigated MHY219, a new HDAC inhibitor, and its potent anticancer activity in human prostate cancer cells. In the present study, we evaluated MHY219 molecular mechanisms involved in the regulation of prostate cancer cell migration. Similar to suberanilohydroxamic acid (SAHA), MHY219 inhibited HDAC1 enzyme activity in a dose-dependent manner. MHY219 cytotoxicity was higher in LNCaP ($IC_{50}=0.67{\mu}M$) than in DU145 cells ($IC_{50}=1.10{\mu}M$) and PC3 cells ($IC_{50}=5.60{\mu}M$) after 48 h of treatment. MHY219 significantly inhibited the HDAC1 protein levels in LNCaP and DU145 cells at high concentrations. However, inhibitory effects of MHY219 on HDAC proteins levels varied based on the cell type. MHY219 significantly inhibited LNCaP and DU145 cells migration by down-regulation of matrix metalloprotease-1 (MMP-1) and MMP-2 and induction of tissue inhibitor of metalloproteinases-1 (TIMP-1). These results suggest that MHY219 may potentially be used as an anticancer agent to block cancer cell migration through the repression of MMP-1 and MMP-2, which is related to the reduction of HDAC1.
( Umasankar De ),( Soma Kundu ),( Nabanita Patra ),( Mee Young Ahn ),( Ji Hae Ahn ),( Ji Yeon Son ),( Jung Hyun Yoon ),( Hyung Ryoung Moon ),( Byung Mu Lee ),( Hyung Sik Kim ) 한국응용약물학회 2015 Biomolecules & Therapeutics(구 응용약물학회지) Vol.23 No.5
Histone deacetylase (HDAC) inhibitors are considered novel agents for cancer chemotherapy. We previously investigated MHY219, a new HDAC inhibitor, and its potent anticancer activity in human prostate cancer cells. In the present study, we evaluated MHY219 molecular mechanisms involved in the regulation of prostate cancer cell migration. Similar to suberanilohydroxamic acid (SAHA), MHY219 inhibited HDAC1 enzyme activity in a dose-dependent manner. MHY219 cytotoxicity was higher in LNCaP (IC50=0.67 μM) than in DU145 cells (IC50=1.10 μM) and PC3 cells (IC50=5.60 μM) after 48 h of treatment. MHY219 significantly inhibited the HDAC1 protein levels in LNCaP and DU145 cells at high concentrations. However, inhibitory effects of MHY219 on HDAC proteins levels varied based on the cell type. MHY219 significantly inhibited LNCaP and DU145 cells migration by downregulation of matrix metalloprotease-1 (MMP-1) and MMP-2 and induction of tissue inhibitor of metalloproteinases-1 (TIMP-1). These results suggest that MHY219 may potentially be used as an anticancer agent to block cancer cell migration through the repression of MMP-1 and MMP-2, which is related to the reduction of HDAC1.
Differentially Rice Protein Expression Between Rice Bran and Endosperm
( Ting-yu Chen ),( De-min Wu ),( Ji-jyun Lai ),( Chang-yue Li ),( Hui-fen Liao ) 한국농업기계학회 2018 한국농업기계학회 학술발표논문집 Vol.23 No.1
Rice (Oryza sativa L.), the major staple food for more than 60% of the world’s population, offer nutritional and health-enhancing properties. Therefore, breeding of new rice species has been fueled by the rising interest in Asian, Latin cuisines, and many countries. In Asia, rice and rice-based ingredients also appeal to both consumers and processors due to their unique combination of taste, nutrition, texture, and biological properties. Proteins and starch in rice are the two major components in rice seed, with approximately 8 and 80%, respectively. Especially in traditional Asian diet, rice seed contributes to about 28-54% of the protein source. The major rice proteins, including structural, metabolic, protective, and storage proteins, serve as sources of nitrogen, sulfur, and carbon for several important physiological functions. In our previous study also demonstrated that rice protein prolamin activated human mononuclear cells to produce cytokines and enhance anti-leukemic immunity. The present study aimed to compare the differentially expression of rice proteins with bran and endosperm by 2-dimentional electrophoresis (2-DE) and mass spectrometric assay. Several protein spots in 2-DE gel with different expression were isolated and identified. The results showed that the major proteins were metabolic, transporter, storage, antioxidant, disease resistant, and development-related proteins. Further investigation to clarify the different manifestations and functions of these proteins might contribute to development of new rice varieties and breeding with unique features.
Study on Springback Properties of Different Orthodontic Archwires in Archwire Bending Process
Jiang Jin-gang,Wang Zhao,Zhang Yong-de,Jiang Ji-xiong,Niu Suo-liang,Liu Yi 보안공학연구지원센터 2014 International Journal of Control and Automation Vol.7 No.12
The archwire bending is one of processes the most frequently used in the orthodontic treatment. Furthermore, the springback of sheet metal, which is defined as elastic recovery of the part during unloading, should be taken into consideration so as to produce formed archwire within acceptable tolerance limits. In this paper, the springback angle of different alloy archwires (including NiTi alloy wire, Beta-Ti alloy wires, Chinese stainless steel wires, and Australian stainless steel wires) were performed by the theoretical calculation based on large deformation theory and the finite element analysis. A series of numerical simulations has been conducted for the bending test, which physically simulates the actual bending of alloy archwire with a robotic apparatus. The finite element analysis of springback is shown to be very consistent with the theoretical calculation results.
De, Umasankar,Son, Ji Yeon,Jeon, Yukyoung,Ha, Song-Yi,Park, Yu Jin,Yoon, Sungpil,Ha, Ki-Tae,Choi, Wahn Soo,Lee, Byung Mu,Kim, In Su,Kwak, Jong Hwan,Kim, Hyung Sik Elsevier 2019 Food and chemical toxicology Vol.123 No.-
<P><B>Abstract</B></P> <P>Plumbagin (5-hydroxy-2-methyl-1,4-naphthaquinone) has displayed antitumor activity <I>in vitro</I> and in animal models; however, the underlying molecular mechanisms have not been fully explored. The aim of this study was to investigate the anticancer effects of plumbagin isolated from <I>Nepenthes alata</I> against MCF-7 breast cancer cells. We examined the cytotoxicity, cell cycle regulation, apoptotic cell death, and generation of intracellular reactive oxygen species (ROS) in MCF-7 cells. Plumbagin exhibited potent cytotoxicity in MCF-7 cells (wild-type p53) compared to that in SK-OV-3 (null-type) human epithelial ovarian cancer cells. Specifically, plumbagin upregulated the expression of p21<SUP>CIP1/WAF1</SUP> in MCF-7 cells, causing cell cycle arrest in the G2/M phase through inhibition of cyclin B1 levels. Plumbagin also significantly increased the ratio of Bax/Bcl-2 and release of cytochrome c, resulting in apoptotic cell death in MCF-7 cells. Furthermore, plumbagin dramatically increased the intracellular ROS level, whereas pretreatment with the ROS scavenger N-acetyl cysteine protected against plumbagin-induced cytotoxicity, suggesting that ROS formation plays a pivotal role in antitumor activity in MCF-7 cells. In mice bearing MCF-7 cell xenografts, plumbagin significantly reduced tumor growth and weight without apparent side effects. We therefore concluded that plumbagin exerts anticancer activity against MCF-7 cells through the generation of intracellular ROS, resulting in the induction of apoptosis via a p53-dependent pathway. This study thus identifies a new anticancer mechanism of plumbagin against p53-dependent breast cancer cells and suggests a novel strategy for overcoming of breast cancer therapy.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Plumbagin, first isolated from <I>N. alata</I>, exhibits anticancer activity in human breast cancer MCF-7 cells. </LI> <LI> ROS generation contributes to the cytotoxicity of plumbagin against MCF7 cells. </LI> <LI> Plumbagin act as a lead molecule for new anticancer drug against breast cancer patients. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
De, Umasankar,Son, Ji Yeon,Sachan, Richa,Park, Yu Jin,Kang, Dongwan,Yoon, Kyungsil,Lee, Byung Mu,Kim, In Su,Moon, Hyung Ryong,Kim, Hyung Sik MDPI 2018 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.19 No.9
<P>We previously discovered a novel sirtuin (SIRT) inhibitor, MHY2256, that exerts anticancer activity through p53 acetylation in MCF-7 human breast cancer cells. We investigated the anticancer activity of MHY2256 against hormone-related cancer, an endometrial cancer with a poor prognosis. The IC<SUB>50</SUB> values of MHY2256 were shown to be much lower than those of salermide, a well-known SIRT inhibitor. Furthermore, MHY2256 significantly reduced the protein expression and activities of SIRT1, 2, and 3, with similar effects to salermide. Particularly, MHY2256 markedly inhibited tumor growth in a tumor xenograft mouse model of Ishikawa cancer cells. During the experimental period, there was no significant change in the body weight of mice treated with MHY2256. A detailed analysis of the sensitization mechanisms of Ishikawa cells revealed that late apoptosis was largely increased by MHY2256. Additionally, MHY2256 increased G1 arrest and reduced the number of cell cyclic-related proteins, suggesting that apoptosis by MHY2256 was achieved by cellular arrest. Particularly, p21 was greatly increased by MHY225656, suggesting that cell cycle arrest by p21 is a major factor in MHY2256 sensitization in Ishikawa cells. We also detected a significant increase in acetylated p53, a target protein of SIRT1, in Ishikawa cells after MHY2256 treatment. In a mouse xenograft model, MHY2256 significantly reduced tumor growth and weight without apparent side effects. These results suggest that MHY2256 exerts its anticancer activity through p53 acetylation in endometrial cancer and can be used for targeting hormone-related cancers.</P>
Ji, Mi-Kyung,Hertsen, Dietmar,Yoon, Doo-Ha,Eum, Heesung,Goossens, Hannelore,Waroquier, Michel,Van Speybroeck, Veronique,D'hooghe, Matthias,De Kimpe, Norbert,Ha, Hyun-Joon Wiley (John WileySons) 2014 Chemistry - An Asian Journal Vol.9 No.4
<P>1-[(1R)-(1-Phenylethyl)]-1-azoniabicyclo[3.1.0]hexane tosylate was generated as a stable bicyclic aziridinium salt from the corresponding 2-(3-hydroxypropyl)aziridine upon reaction with p-toluenesulfonyl anhydride. This bicyclic aziridinium ion was then treated with various nucleophiles including halides, azide, acetate, and cyanide in CH3CN to afford either piperidines or pyrrolidines through regio- and stereoselective ring opening, mediated by the characteristics of the applied nucleophile. On the basis of DFT calculations, ring-opening reactions under thermodynamic control yield piperidines, whereas reactions under kinetic control can yield both piperidines and pyrrolidines depending on the activation energies for both pathways.</P>