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Cimiside E Arrests Cell Cycle and Induces Cell Apoptosis in Gastric Cancer Cells
Lian Yu Guo,Eun Ji Joo,손건호,Su Jin Jeon,Sehyun Jang,신은명,Hong Yu Zhou,Yeong Shik Kim 대한약학회 2009 Archives of Pharmacal Research Vol.32 No.10
Cimiside E was isolated from the Cimicifuga heracleifolia Komarov extract, which has been previously demonstrated to possess apoptotic action on gastric cancer cells. The IC50 value of cimiside E on gastric cancer cells for 24 h was 14.58 μM. The mechanism of apoptosis was further elucidated through western blot, RT-PCR, morphology, Annexin V-FITC/PI staining and cell cycle analysis. Cell cycle arrest was induced by cimiside E in S phase at a lower concentration (30 μM) and G2/M phase at higher concentrations (60 and 90 μM). Cimiside E mediated apoptosis through the induction of the caspase cascade for both the extrinsic and intrinsic pathways. These findings suggest that cimiside E may be an effective chemopreventive agent against cancer.
ON THE END EXTENDING IN THE HILBERT-HUANG TRANSFORM
Da Ji Huang,Jin Ping Zhao,Ji Lian Su 한국해안해양공학회 1999 학술강연회 발표논문초록집 Vol.1 No.1
Hilbert-Huang transform is powerful method of nonlinear and non-stationary time series analysis. Two approaches, mirror periodic and extrema extending method, have been developed for handling the end effects of Hilbert-Huang transform. The mirror periodic method is based on the data distribution at ends. Two mirrors are placed at the extrema of both ends. The data within the mirrors are extended with mirror reflection to obtain a periodic sequence. With this method, the end effects of empirical mode decomposition and Hilbert transform are solved once forever. The mirror periodic method can get best results by placed the mirrors at the extrema, which have symmetric property of the data. Extrema extending method is implemented by adding two maxima and minima at the ends, which are defined by the two consecutive extrema of the data at ends. It is easy to perform and has equally well behalf as mirror periodic method. The extrema method is superior to the mirror periodic method when the data has strong asymmetric waveform.
Wang, Hang-Hui,Song, Yi-Xin,Bai, Min,Jin, Li-Fang,Gu, Ji-Ying,Su, Yi-Jin,Liu, Long,Jia, Chao,Du, Lian-Fang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.3
The aim was to determine whether ultrasound targeted microbubble destruction (UTMD) promotes dual targeting of HSP72 and HSC70 for therapy of castration-resistant prostate cancer (CRPC), to improve the specific and efficient delivery of siRNA, to induce tumor cell specific apoptosis, and to find new therapeutic targets specific of CRPC.VCaP cells were transfected with siRNA oligonucleotides. HSP70, HSP90 and cleaved caspase-3 expression were determined by real-time quantitative polymerase chain reaction and Western blotting. Apoptosis and transfection efficiency were assessed by flow cytometry. Cell viability assays were used to evaluate safety. We found HSP72, HSC70 and HSP90 expression to be absent or weak in normal prostate epithelial cells (RWPE-1), but uniformly strong in prostate cancerous cells (VCaP). UTMD combined with dual targeting of HSP72 and HSC70 siRNA improve the efficiency of transfection, cell uptake of siRNA, downregulation of HSP70 and HSP90 expression in VCaP cells at the mRNA and protein level, and induction of extensive tumor-specific apoptosis. Cell counting kit-8 assays showed decreased cellular viability in the HSP72/HSC70-siRNA silenced group. These results suggest that the combination of UTMD with dual targeting HSP70 therapy for PCa may be most efficacious, providng a novel, reliable, non-invasive, safe targeted approach to improve the specific and efficient delivery of siRNA, and achieve maximal effects.