Skin Permeability of Porcine Placenta Extracts and Its Physiological Activities

Jeunghi Han,Mi-ryung Kim,Yooheon Park,Yang Hee Hong,Hyung Joo Suh 한국축산식품학회 2013 한국축산식품학회지 Vol.33 No.3

We investigated the skin permeability and various biological activities of porcine homogenate of placenta (HP) with the highest protein contents (452.89μg/mg). The content of protein in subcritical extract of HP (SPE) was decreased from the initial content of 452.9μg/mg to 262.7μg/mg at 3 h subcritical extract. The contents of amino type nitrogen (A-N) were sharply increased from 35.1 μg/mg of initial content to 305.9μg/mg at 3 h subcritical extract. The HP showed a noticeable activity in terms of antioxidant capacity for ferric reducing antioxidant power (FRAP) assay and especially for 2, 2` Azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) method. HP, SPE-0.5, SPE-2 and SPE-3 showed inhibitory effect on elastase activities with an IC50 of 46.1, 42.9, 31.6 and 34.7μg/mL, respectively. SPEs showed more significantly inhibitory effect than HP (p

Protective Effect of Porcine Placenta Extracts against UVBInduced Photoaging in Hairless Mouse

JeungHi Han,Yooheon Park,Yang Hee Hong,Seungsik Chung,Hyung Joo Suh 한국식품영양과학회 2013 한국식품영양과학회 학술대회발표집 Vol.2013 No.11

Enhancement of Cosmeceutical Functionality of Swine Placental Collagen by Subcritical Extraction

JeungHi Han,Kyung Soo Ra,Mingzhe Pang,Eu Deum Park,Kwang Min Kim,Hyung Joo Suh 한국식품영양과학회 2012 한국식품영양과학회 학술대회발표집 Vol.2012 No.10

Skin Permeability of Porcine Placenta Extracts and Its Physiological Activities

Han, JeungHi,Kim, Mi-Ryung,Park, Yooheon,Hong, Yang Hee,Suh, Hyung Joo Korean Society for Food Science of Animal Resource 2013 한국축산식품학회지 Vol.33 No.3

We investigated the skin permeability and various biological activities of porcine homogenate of placenta (HP) with the highest protein contents (452.89 ${\mu}g/mg$). The content of protein in subcritical extract of HP (SPE) was decreased from the initial content of 452.9 ${\mu}g/mg$ to 262.7 ${\mu}g/mg$ at 3 h subcritical extract. The contents of amino type nitrogen (A-N) were sharply increased from 35.1 ${\mu}g/mg$ of initial content to 305.9 ${\mu}g/mg$ at 3 h subcritical extract. The HP showed a noticeable activity in terms of antioxidant capacity for ferric reducing antioxidant power (FRAP) assay and especially for 2,2'-Azinobis- (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) method. HP, SPE-0.5, SPE-2 and SPE-3 showed inhibitory effect on elastase activities with an $IC_{50}$ of 46.1, 42.9, 31.6 and 34.7 ${\mu}g/mL$, respectively. SPEs showed more significantly inhibitory effect than HP (p<0.05). The skin permeability of the SPEs was higher than that of the HP. SPE-3 showed highest skin permeation and the permeability was significantly higher than that of HP. SPE-2 also showed significantly higher permeation than HP after 4 h. As expected, increase of extraction time significantly increased skin permeability in the subcritical extract of HP (SPE). From these results, in terms of cost and source availability, porcine placenta extracted with subcritical extraction has advantages over untreated PE and have potential as a cosmetic ingredient.

패각 칼슘 입자 크기에 따른 흡수율

한정희(JeungHi Han),최현선(Hyeon-Son Choi),나경수(Kyung Soo Ra),정승식(Seungsik Chung),서형주(Hyung Joo Suh) 한국식품영양과학회 2014 한국식품영양과학회지 Vol.43 No.3

본 연구에서는 패각에서 유래하는 칼슘의 이용가치를 높이고자 분쇄한 패각 칼슘을 입자 크기별로 제조하여 이온화율과 흡수율을 조사하였다. 패각 칼슘분말 현탁액을 알코올 중에서 비중차이별로 분리하고 입자 크기를 측정하여 4개의 시료군으로 나누었다; NC(일반 패각 칼슘), C-1(1,000 rpm 상등액), C-2(2,000 rpm 상등액), C-3(3,000 rpm 상등액). 이들의 입자 크기는 각각 2,280.3±64 nm, 521.3±83.3nm, 313.9±29.5 nm, 280±3.4 nm를 보였다. 각 칼슘군들을 대상으로 이온화 정도를 측정한 결과 C-3군이 오차범위내에서 다른 군들에 비해 이온화율이 다소 증가했다. In vitro상에서 dialysis 막을 이용한 나노칼슘의 투과율을 측정한 결과 나노칼슘의 입자 크기가 작을수록 막 투과성이 증대됨을 확인할 수 있었다. 한편 rat의 내장주머니 막(intestinal sac)을 이용하여 장내 환경별 나노칼슘의 투과율을 측정한 결과 C-3군을 제외한 모든 군에서 십이지장(pH 4.2)과 공장(pH 6.2) 환경보다는 회장(pH 7.2)에서의 나노칼슘의 장투과율이 비교적 높게 나타난 반면 C-3군에서는 십이지장환경에서의 투과율이 다소 높았다. 특히 십이지장 환경에서의 샘플 간 투과율을 비교할 때 C-2와 C-3군이 가장 높은 투과율을 보였고 NC 칼슘이 가장 낮은 투과율을 나타내어 입자 크기가 작을수록 장 투과율이 증가함을 확인할 수 있었다. 위의 결과를 종합해볼 때, 패각에서 유래하는 칼슘을 적절한 가공처리를 거쳐 나노칼슘으로 만들면 칼슘의 이온화율과 장내흡수율을 향상시킬 수 있는 것으로 사료된다. In this study, we examined the ionization rate and permeability of nanocalcium prepared from oyster shells with various particle sizes. Four particle sizes of the calcium samples were prepared by centrifugation according to their density disparity in alcoholic solution: NC (normal calcium), C-1 (supernatant of 1,000 rpm), C-2 (supernatant of 2,000 rpm), and C-3 (supernatant of 3,000 rpm). Particle sizes of NC, C-1, C-2, and C-3 were 2,280.3±64.3 nm, 521.3±83.3 nm, 313.9±29.5 nm, and 280.0±3.4 nm, respectively. C-3 showed a slight increase in ionization rate compared with the other calcium samples, but their differences were not significant. Dialysis membrane-employed analysis showed that nanocalcium permeability increased as its particle size smaller; 32% of C-3 nanocalcium was transported to the outside of the membrane, whereas C-1 showed a 25% transport rate. We determined the permeability of the nanocalciums by using rat intestinal sacs, in order to provide different intestinal environments depending on pH level. Nanocalcium generally showed a higher permeability at pH 7, which represents an ileum environments compared to the duodenum and jejunum environments at pH 4.2 and pH 6.2, respectively. However, C-3 calcium showed the highest permeability, followed by C-2, C-1 and NS calciums. This result shows that the size of calcium positively affected its permeability in the intestinal sac. Taken together, nano-sized calcium derived from discarded oyster shell shows improved permeability in intestinal environments.

Nano-Calcium Ameliorates Ovariectomy-Induced Bone Loss in Female Rats

Choi, Hyeon-Son,Han, JeungHi,Chung, Seungsik,Hong, Yang Hee,Suh, Hyung Joo Korean Society for Food Science of Animal Resource 2013 한국축산식품학회지 Vol.33 No.4

In this study, we examined the effects of organic types of calcium derived from oyster shell (OS-Ca) and nano-calcium (Nano-Ca) on the bio-availability and physiological responses associated with bone health in ovariectomised rats. Increased body weight, which is one of the physiological effects of ovary removal, was significantly recovered by Nano-Ca treatment (p<0.05). The reduced calcium level in the liver in ovariectomised rat was increased significantly with OS-Ca and Nano-Ca treatment (p<0.05), suggesting improved calcium bio-availability. Alkaline phosphatase (ALP), osteocalcin, and deoxypyridinoline (DPD) were analysed as biochemical markers of bone metabolism and health in the presence or absence of OSCa and Nano-Ca. ALP, osteocalcin, and DPD levels increased following ovary removal and tended to decrease after treatment with Nano-Ca, indicating that Nano-Ca induces favourable bone metabolism. This result was reflected in the recovery of bone mineral density (BMD) and bone mineral content (BMC) of the femur after Nano-Ca treatment following ovary removal. Taken together, our data show that the tested calcium treatments, especially using Nano-Ca, enhanced the bioavailability or absorption of calcium and positively affected bone metabolism in ovariectomised rats.

Nano-Calcium Ameliorates Ovariectomy-Induced Bone Loss in Female Rats

Hyeon-Son Choi,JeungHi Han,Seungsik Chung1,Yang Hee Hong,and Hyung Joo Suh 한국축산식품학회 2013 한국축산식품학회지 Vol.33 No.4

In this study, we examined the effects of organic types of calcium derived from oyster shell (OS-Ca) and nano-calcium (Nano-Ca) on the bio-availability and physiological responses associated with bone health in ovariectomised rats. Increased body weight, which is one of the physiological effects of ovary removal, was significantly recovered by Nano-Ca treatment (p<0.05). The reduced calcium level in the liver in ovariectomised rat was increased significantly with OS-Ca and Nano-Ca treatment (p<0.05), suggesting improved calcium bio-availability. Alkaline phosphatase (ALP), osteocalcin, and deoxypyridinoline (DPD) were analysed as biochemical markers of bone metabolism and health in the presence or absence of OSCa and Nano-Ca. ALP, osteocalcin, and DPD levels increased following ovary removal and tended to decrease after treatment with Nano-Ca, indicating that Nano-Ca induces favourable bone metabolism. This result was reflected in the recovery of bone mineral density (BMD) and bone mineral content (BMC) of the femur after Nano-Ca treatment following ovary removal. Taken together, our data show that the tested calcium treatments, especially using Nano-Ca, enhanced the bioavailability or absorption of calcium and positively affected bone metabolism in ovariectomised rats.

Protective Effect of Ginseng Leaf against UVB-Induced Photoaging in Hairless Mouse

Mi-Ryung Kim,JeungHi Han,Yoo-Kyung Kim,Yooheon Park,Sun Young Kim,Hyung Joo Suh 한국식품영양과학회 2012 한국식품영양과학회 학술대회발표집 Vol.2012 No.10