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Jer-Yiing Houng,Jui-Ching Tseng,Hsia-Fen Hsu,Jiumn-Yih Wu 한국화학공학회 2008 Korean Journal of Chemical Engineering Vol.25 No.6
This study investigated the kinetic characteristics of asymmetric bioreduction of ethyl 4-chloro acetoacetate (ECA) to produce (S)-4-chloro-3-hydroxybutyric acid ethyl ester (S-CHBE) by baker’s yeast in a water-organic solvent biphasic system. Exactly how several organic solvents affect reaction performance was studied first. Among the solvents tested, petroleum ether exhibited the optimum reaction efficiency. Compared with the aqueous system, reaction yield was enhanced from 74.5% to 84.0%, and the product’s ee increased from 82.3% to 88.0% after 10% petroleum ether was added. The kinetic behavior of asymmetric bioreduction of ECA in the petroleum ether-water biphasic system was then examined by using a mathematical model. Kinetic analysis reveals that the maximal reaction rate and affinity between the substrate and the biocatalyst were both lower in the biphasic system than in the aqueous system. Additionally, the substrate inhibition effect was greater in this biphasic system than in the aqueous system. However, the ratio of the formation rate for producing S-CHBE to that for producing R-CHBE in the biphasic system was significantly higher than that in the aqueous system. Moreover, adding petroleum ether reduced spontaneous ECA degradation markedly. These two kinetic characteristics explain why the biphasic system exhibited a higher yield and a better product’s ee (enantiomeric excess) than the aqueous system.
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Un-symmetric Input Temperature Control by using Fuzzy Sliding Mode Controller with Gain Auto-tuning
Shiuh-Jer Huang,Hung-Yi Chen 제어·로봇·시스템학회 2014 International Journal of Control, Automation, and Vol.12 No.2
In order to obtain the desired product quality, temperature is an important control parameter in chemical and semiconductor manufacturing processes. Generally, the temperature control system has nonlinear time-varying, slow response speed, time-delay and un-symmetric control input dynamic characteristics. It is difficult to accurately establish the dynamic model for designing a general purpose temperature controller to achieve good control performance. Here a model-free fuzzy sliding mode control strategy is employed to design an intelligent temperature controller with gain-scheduling scheme or gain auto-tuning algorithm for a closed chamber with heater one-way input only. The concept of gain scheduling is employed to adjust the mapping ranges of the input and output fuzzy membership functions during the control process for improving the transient and steady-state control performances. The experimental results show that the steady state error of the step input response is always less than 0.2oC without overshoot by using this intelligent control schemes. It is suitable for industrial temperature control systems.
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Adaptive neural network controller for the molten steel level control of strip casting processes
Hung-Yi Chen,Shiuh-Jer Huang 대한기계학회 2010 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.24 No.3
The twin-roll strip casting process is a steel-strip production method which combines continuous casting and hot rolling processes. The production line from molten liquid steel to the final steel-strip is shortened and the production cost is reduced significantly as compared to conventional continuous casting. The quality of strip casting process depends on many process parameters, such as molten steel level in the pool, solidification position, and roll gap. Their relationships are complex and the strip casting process has the properties of nonlinear uncertainty and time-varying characteristics. It is difficult to establish an accurate process model for designing a model-based controller to monitor the strip quality. In this paper, a model-free adaptive neural network controller is developed to overcome this problem. The proposed control strategy is based on a neural network structure combined with a sliding-mode control scheme. An adaptive rule is employed to on-line adjust the weights of radial basis functions by using the reaching condition of a specified sliding surface. This surface has the on-line learning ability to respond to the system’s nonlinear and time-varying behaviors. Since this model-free controller has a simple control structure and small number of control parameters, it is easy to implement. Simulation results, based on a semiexperimental system dynamic model and parameters, are executed to show the control performance of the proposed intelligent controller. In addition, the control performance is compared with that of a traditional PID controller.
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Chang, Shih-Yi,Tso, Tai-Ly,Lo, Jiunn-Gung,Huang, Jer-Luen,Lin, Cheng Ming 한국분석과학회 1995 분석과학 Vol.8 No.4
Toxic air pollutants are investigated in a petrochemical industrial park in Taiwan by using a movable open path FTIR. The results show the qualitative and quantitative analysis of emission gases from plants, and also provide the emission rate of different compounds. More than twenty compounds under usual operation are found from this industrial park. The concentration variation with time can be correlated exactly with wind direction. It means that by changing the measuring points, the source of emission can be unambiguously identified. An EPA proved PAL model is applied to estimate the emission rate of either a point or an area source. Local atmospheric stability is determined by releasing the $SF_6$ tracer. The origins of errors come mainly from the uncertainty of source's configuration and the variation of meteorological condition. Through the continuous measurement (half an hour base in this study) of OP-FTIR sensor, the maximum value of emission rate and the annual amount of emission can be derived. The emission rate of the measured toxic gases are derived by the model technique and the results show that the emission amount are in the order of ten to hundred tons per year.
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A MULTI-HIERARCHICAL ANALYSIS OF ONLINE SERVICE RECOVERY
Tzu-En Lu,Yi-Hsuan Lee,Chan Hsiao,Jer-Wei Hsu 글로벌지식마케팅경영학회 2018 Global Marketing Conference Vol.2018 No.07
This study incorporates brand equity and brand identity, two vital factors influencing customer-brand perception, to explore the process from service recovery to perceived justice. This study adopted HLM as the research framework because brand perception differs at various levels and adopting HLM allows us to precisely understand the influence of brand identity and brand equity on the relationship between service recovery and perceived justice. The contributions of this study are listed as follows: a) online service recovery can positively affect customer-perceived justice; b) brand equity at the organizational level can undermine the relationships that courtesy and compensation have with perceived justice; c) brand identity at the individual level can undermine the relationships that courtesy and compensation have with perceived justice; and d) using hierarchical linear modeling can precisely measure the relationship between organizations and customers; therefore, this method is innovative and can contribute to the research on online service recovery.
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The Beneficial Effects of Raffinee in Permanent Occulted Stroke Mice
Chun-Ching Chiu,Jer-Min Lin,Li-Yi Wu,Tsai-Ching Hsu,Bor-Show Tzang 한국식품영양과학회 2019 Journal of medicinal food Vol.22 No.12
Ischemic stroke is a major cause of disability and mortality globally. Although thrombolytic therapy is routinely adopted in cases of ischemic stroke, various alternative natural neuroprotectants are also used as effective adjuvant therapies to recover neurofunction following ischemic stroke. Raffinee, a natural fermented product with strong antioxidant and neuroprotective activities, has antiatherogenic effects in animals and has exhibited neuroprotective effects in a clinical trial by recovering motor and sensory function following spinal cord lesion. This study reveals the advantageous effects of Raffinee on PC12 cells by decreasing hypoxia-induced apoptosis in mice with permanent middle cerebral artery occlusion (pMCAO) by increasing the levels of neurotrophic factors such as S100β, reducing serum inflammatory factors such as matrix metalloproteinases (MMP)-9/MMP-2 ratio, tumor necrosis factor-α, and interleukin (IL)-6 level, and increasing IL-10 levels. Significantly reduced brain infarct volume along with a favorable survival ratio was observed for pMCAO mice that received Raffinee, suggesting a neuroprotective potential of Raffinee in cases of acute ischemic stroke by suppressing apoptosis.
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( Ming-lung Yu ),( Chao-hung Hung ),( Yi-hsiang Huang ),( Cheng-yuan Peng ),( Chun-yen Lin ),( Pin-nan Cheng ),( Rong-nan Chien ),( Shih-jer Hsu ),( Chen-hua Liu ),( Jee-fu Huang ),( Chung-feng Huang 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1
Aims: The current study aims to elucidate the treatment efficacy (defined as undetectable HCV RNA throughout 12 weeks of post-treatment follow-up, SVR12) and safety DCV/ASV plus ribavirin for 12 weeks in HCV-1b patients without NS5A RAS. Methods: This is a single-arm, open-label phase 2 study. Seventy directly acting antivirals (DAA)-naïve HCV-1b patients without L31/Y93 RAS are planned to receive daclatasvir (60 mg/ day) and asunaprevir (100 mg twice daily) plus weight-based ribavirin (1000-1200 mg/day) for 12 weeks. After treatment they were followed up for 12 weeks. Results: As of 31 Oct 2017, 58 eligible patients are allocated to treatment, with a mean age of 59.3 years and female predominance (67.2%, 39/58). The mean HCV RNA was 5.87+0.77 log10 IU/mL; 23 patients (39.7 %) had significant hepatic fibrosis (>F2). In the modified intention-to-treat analysis, the rate of undetectable HCV at week 1, week 2, week 4, week 8 and endof- treatment was 25 % (14/56), 84.8 % (39/46), 100 % (46/46), 100 % (38/38) and 100 % (27/27), respectively. Undetectable HCV RNA were observed in all of the patients with HCV RNA assessable 4 weeks (SVR4, 18/18) and 12 weeks (SVR12, 12/12) post treatment. None of the 18 patients who completed the 12-week treatment experienced relapse during post-treatment follow-up. The most common adverse event was fatigue (78.3 %), followed by pruritus (65.2 %) and dizziness (52.2 %), of which were considered as ribavirin related. None of the participating subjects withdrew treatment or follow-up throughout the trial peroid. Three serious adverse events were reported which included urosepsis, appendicitis and left ureteral stone. All were unrelated to the investigating drugs. Conclusions: 12 weeks of DCV/ASV plus ribavirin was highly effective and safe in HCV-1b patients without NS5A RAS in the interim analysis. The satisfactory results would be anticipated in the full patient set.
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Chin-Fa Hwang,Jen-Han Chang,Jer-Yiing Houng,Cheng-Chih Tsai,Chien-Ku Lin,Hau-Yang Tsen 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.4
An immune-enhancing strain, Lactobacillus plantarum Pi06, isolated from a healthy infant was used for biomass production following optimization of the medium in shake-flask culture. Preliminary studies showed that commercial MRS medium and cultivation under static conditions generated higher biomass production than four other tested media with or without a shaking condition. The selected medium composition, consisting of glucose,yeast extract, soy peptone, ammonium citrate, and corn steep liquor, was further optimized using a systematic method that integrated the Taguchi array design and the Box–Behnken method. The response effects of these factors were first investigated using Taguchi design under an L16 (45) array. The suggested medium composition,derived from Statistica 7.1 using the Taguchi design, was applied to cultivate cells and a biomass of 7.16 g dry cell weight (DCW)/L was obtained. Response surface methodology based on the Box–Behnken method for the three response variables of glucose, yeast extract, and corn steep liquor was then used to further increase the biomass level to 8.94 g DCW/L. The resulting optimum medium consisted of 35 g/L glucose, 35 g/L yeast extract, and 40 mL/L corn steep liquor. Compared with the initial medium, the biomass yield was improved from 4.31 to 8.94 g DCW/L, an enhancement of approximately 107%.
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