사염화탄소로 유발된 간손상에서의 효소 활성도의 변화로 본 홍화자 분획물의 간손상 보호 작용

정춘식,정기화,정정숙 德成女子大學校 藥學硏究所 1999 藥學論文誌 Vol.10 No.1

본 연구소에서는 사염화탄소로 유발된 간손상에 대한 홍화자 메탄올 추출물과 이를 계통 분획한 분획물의 보호 효과를 이미 보고하였다. 본 실험에서는 혈장과 조직 생화학적 분석으로 홍화자 분획물의 간보호 효과를 확인하며, 간보호 작용에 대한 기전을 살펴보기 위하여 약물 대사 효소에 미치는 영향을 측정하였다. 홍화자 butanol subfractions 중 BS-5 분획물은 CCl_4로 간손상을 유발시킨 흰쥐에서의 혈장 ALT와 AST 활성과 간조직 중 cholesterol and triglyceride를 감소시켰으며, 이러한 BS-5 분획물의 CCl_4로 유발된 간손상에 대해 보호 작용은 cytochrome P450 감소와 glutathione S-transferase의 활성 증가에 기인된 것으로 생각된다. Previous studies have shown that methanol extract and its butanol fraction of Carthamus tinctorius L. Semen have the hepatoprotective effect on the CCl_4-induced hepatotoxicity. The hepatoprotective effect of subfractions has been evaluated by analyzing blood and hepatocyte biochemical analyses and biotransformation enzyme analyses. Treatment of BS-5 subfraction has significantly decreased the activities of alanine aminotransferase and aspartate aminotransferase. In addition, the levels of cholesterol and triglyceride in liver have been decreased as compared with that of CCl_4 treated rats. The hepatoprotective effect of BS-5 subfraction on the CCl_4-induced hepatotoxicity would be mediated of the attenuation of the level of cytochrome P450 and the enhancement of the activity of glutathion S-transferase.

홍화자 분획물이 사염화탄소 유발 간손상 흰쥐에서 지질과산화와 oxygen free radical 제거 효소 활성도에 미치는 영향

정기화,정춘식,정정숙 德成女子大學校 藥學硏究所 1999 藥學論文誌 Vol.10 No.1

저자들은 사염화탄소로 유발된 간손상에 대한 홍화자 메탄올 추출물과 이를 계통 분획한 분획물의 보호 효과를 이미 보고하였다. 본 실험에서는 홍화자 분획물의 간보호 작용에 대한 기전을 살펴보기 위하여 지질과산화와 oxygen free radical 제거 효소 활성을 측정하였고 조직병리학적 분석을 통하여 간보호 효과를 확인하였다. 홍화자 BS-5 분획물은 CCl_4로 간손상을 유발시킨 흰쥐에서 superoxide dismutase의 활성도를 유의적으로 증가시켰으며, in vitro에서의 1, 1-diphenyl -2-picrylhydrazyl radical를 직접적으로 제거하였다. 또한 조직병리학적 분석에서도 홍화자 BS-5분획투여군은 CCl_4 투여로 인한 중심정맥 주변의 ballooning degeneration, 염증 세포 침윤 및 괴사를 완화시켜 간손상 보호 작용을 나타내었으며, 이는 free radical scavenging 작용에 의한 것으로 생각된다. Previous studies have shown that methanol extract and its butanol fraction of Carthamus tinctorius L. Semen have the hepatoprotective effect on the CCl_4-induced hepatotoxicity. The hepatoprotective effect fo the subfractions of butanol fraction has been evaluated by analyzing oxygen free radical scavenging enzyme activities and histopathological examinations. In BS-5 subfraction treated group, the activity of superoxide dismutase has been significantly increased as compared with that of CCl_4 treated rats. Antioxidant activity has been evaluated by the examination of the scavenging ativity against 1,1-diphenyl-2-picrylhydrazyl radical. BS-5 subfraction has shown strong antioxidant activities. The histopathological examination showed that the treatment of BS-5 subfraction has relieved the ballooning degeneration of hepatocytes which had been generated by CCl_4. It appears that the protective effect of BS-5 subfraction would be mediated of the attenuation of lipid peroxidation by acting as a free radical scavenger. which were based on the increase of superoxide dismutase activity.

청각장애초등학생을 위한 음악교육프로그램 개발 연구

이드보라ㆍ남선화ㆍ정미라(Debora LeeㆍNam Sun WhaㆍJeong Mi ra) 한국특수교육학회 2016 특수교육학연구 Vol.50 No.4

合成洗劑가 빨간집모기의 生態, 生理學的 特性에 미치는 影響

金正和,李炯來,朴垠澈,李容揆,崔觀善 충북대학교 농업과학기술연구소 1995 農業科學硏究 Vol.12 No.2

It is considered a general experience that migration from rural to urban areas will go on in any developing countries pursuing economic development through industralization. In Korea, migration is considered one of the serious socio- economic problems because it has been made excessively fast than any other countries' experiences. The socio- economic problems originates in migration can be classified in two ways ; the one is the overpopulated problems of urban areas and emptied villages problems of rural areas, and the other is the shartage agricultural labor farce problems symbolized by the increasing idle cultivated land. To relieve migration effectively, the developing policies should be converted from pursuing fast industralization and urbanization to balance growth between areas and industries. Moreover problem solving political measure should be converted from partial and corresponding approaches to total approaches containing economic, social and institutional side-view.

농촌에 서식하는 빨간집모기의 살충제 저항성에 대하여

金正和,朴垠澈 충북대학교 농업과학기술연구소 1996 農業科學硏究 Vol.13 No.-

The expriments were carried out elucidate the different resistance of Temephos and Dichlorvos against the Mosquito, Culex pipiens pallens, collected in a rural community using various methods such as bioassay, electrophoresis and filter paper test. The experimntal results were as following 1 . Bioassay The LC50 and LC95 Values of each strains of the mosquito were determined using profit analysis. Insecticide resistance of each strains was collected from July to September in 1995. Resistant ratio(RR50) of cowshed strain showed 1.03 and 0.44 times to the Temephos and Dichlorvos treated. Resistant ratio(RR50) of henhouse strain showed 2.14 and 0.93 times to the temephos and Dichlorvos treated. Resistant ratio(RR 50) of pigpen strain showed 1.46 and 1.28 times to the Femephos and Dichlorvos treated. 2. Enzyme activity 1) Esterase isozyme pattern Est-αaβ1 was detected in all strains tested by elecgrophorsis. The activity was the highest in cowshed strain and the lowest in pigpen strain. 2) Filter paper test Esterase-α activities showed higher 1.41 and 1.40 times to the cowshed and heenhouse strain treated than that of the control, respectively, by filter paper test.

감꼭지나방 생태적 특성 및 방제약제 선발

김정화,김길하,조수원,박은철,김순섭,황태구,최상기 충북대학교 첨단원예기술개발연구센터 1999 연구보고서 Vol.4 No.-

경남지역과 충북지역 감나무의 재배, 해충 방제실태를 농가 방문하여 설문조사 한 결과 감 재배경력은 경남지역이 평균 10년이며, 충북지역은 평균 5년 정도이다. 감 재배 주수는 대다수 농가가 20주 이상이고, 재배관리에 대하여 자문을 받는 곳은 농업센터나 농약사 및 연구소 등이었다. 김 품종은 경남지역에서는 부유와 서촌조생, 충북지역에서는 등시와 월하 등을 재배하고 있었다. 시비종류는 이른봄 에 퇴비나 복합비료를 주었고, 감나무 생장 중 열매나 저장 중 열매에 발생하는 해충이 발견되었다. 해충방제를 위해 살충제를 살포하고 있는데 주로 감꼭지나방, 진딧물, 잎말이나방 및 깍지벌레 등으로 파마치온, 파단, 코니도, 신파마치온, 수프라사이드 등을 살포하였다. 감꼭지나방(Stathmopoda masinissa)의 발육생태를 조사하기 위하여, 감꼭지나방을 야외에서 채집하여 온도±1℃, 광주기 16L:8D, 상대습도 50~60%에서 실내 사육하였다. 각 층태별 기간은 알 7.4일, 유충기간 34.7일, 용 15.5일이었다. 각 령기간은 1령 3.5일, 2령 4.2일, 3령 5.2일, 4령 6.5일, 5령 15.4일이었으며, 특히 유층의 두폭을 측정하여 평균한 결과는 1령 0.20mm, 2령 0.40mm, 3령 0.65mm, 4령 0.87mm, 5령 1.07mm일 이었다. 성충의 수명은 수컷이 6.2일, 암컷이 10.1일 이었다. 용화율은 68.0% 우화율은 59.9%이었다. A survey in Kyungnam and chungbuk areas revealed that the average number of years for persimmon tree farming is 10 and 5 years, respectively. The number of trees per orchard is at least over 20, and the farmers have been usually advised through an agricultural center, an agrochemical store, or agrochemical research center. The varieties of the persimmons they grow are Buyu and Seocheon in Kyungnam, and Doogsi and Wolha in Chungbuk. The kind of fertilizers they sprayed in early spring is either compost or composite fertilizer. persimmon pests are often found in persimmon fruits while on a tree or in storage. Phamathion, Padan, Konido, Sinpamathion, and/or supuraside is usually used to control persimmon fruit moths, aphides, leafrollers, and scale insects. To understand the developmental ecology of the persimmon fruit moth, Stathmopoda masinissa Meyrick, we collected and reared them under the laboratory condition which is at 25±1℃ of temperature, 16L:8D of photoperiod, and 85% of relative humidity. As a result, the developmental period of egg, larva, and pupa were 7.40, 34.7, and 15.5 days, respectively. The average period of each larval instar was 3.50, 4.17, 5.17, 6.46, and 15.42 days, respectively, and the average head capsule width was 0.20, 0.40, 0.65, 0.87, and 1.07mm, respectively. The average adult longevity was 6.2 days for males and 10.1 days for females. The pupation rate and the emergence rate were 68.0% and 59.9%, respectively.

昆蟲의 酵素分析에 의한 環境汚染比較에 관한 硏究

金正和,李仁煥,李容揆 충북대학교 농업과학기술연구소 1992 農業科學硏究 Vol.10 No.1

The experiment was conducted to elucidate the differences of several mosquito strains(Culex pipiens pallans Coquillet : Mushimcheon, Daecheongdam, Oksan, Weolacksan) to various insecticides using the methods such as bioassay, electrophoresis, and filter paper test. And the results are summarized as follows. 1. In LC50, Mushimcheon strain showed the highest insecticidal resistance while that of Weolacksan strain was the lowest. Resistant ratio(RR50) of mosquito srains to temephos was very high while all strains showed very low rate to propoxur. In LC95, insecticidal resistance to temephos was very high in Oksan strain while Daecheongdam strain was very low. 2. Est-Bl was detected in all strains tested by electrophoresis. And the activity was very high in Oksan strain and very low in Daecheongdam strain. 3. Esterase activity, determined by filter paper test and digital densitometry, was very high in Oksan strain and that of Daecheongdam was very low. 4. The results obtained thus far indicated that the mosquito strains that inhabit in Oksan and Mushimcheon region where respective agricultural chemicals and insecticides to control medical insects are heavily applicated are more contaminated by insecticidal chemicals than other regions.

집파리의 殺蟲劑 低抗性과 에스테라제 活性 比較

金正和,李炯來,李容揆 충북대학교 농업과학기술연구소 1994 農業科學硏究 Vol.12 No.1

The experiment was conducted to elucidate the differences of isozyme pattern and activity to esterase in susceptible and chlorpyrifos-(Rc), dichlorvos-(Rd) and permethrin-(Rp) resistant strains of female house flies(Musca domestica L.) using the methods of agar gel electrophoresis and filter paper test. And the results were summarized as follows. 1.Esterase isozyme showed eleven bands in the susceptible and three resistant strains by agar gel electrophoresis. 1) Esterase isozyme bands were Estα1β1, Estα2β2, Estα3β3, -Estα1β1 in the head, Estα1β1, Estα2β2, Estβ6, -Estα1β1, -Estα4β4 in the thorax and Estα1β1, Estα2β2, -Estα1β1, -Estα5β5 in the abdomen of the susceptible and resistant strains. 2) Estα5β5 was detected in all part(head, thorax, abdomen) of resistant strains, and Estα4β4 was not detected in the head and thorax of resistant strains. 3) -Estα2β2 showed more high activity in the thorax of Rc strain than the other strains. 4) Only -Estα3β3 was detected in all part of Rd strain. 5) -Estα2β2 was not detected in all part, and Estα3β3, Estα4β4, Estβ6 were not detected in the abdomen of Rp strain, however, those were detected in susceptible strain. 2.Esterase-α and -β activities appeared in the head, thorax and abdomen of susceptible and resistant strains by filter paper test. 1) Esterase-α showed more high activity than esterase-β. 2) Esterase-α activities were 1.15, 1.18 and 1.03 times in the head, 0.87, 1.18 and 0.89 times in the thorax and 0.82, 1.51 and 1.30 times in the abdomen of Rc, Rd and Rp strains to the susceptible strain, respectively. 3) Esterase-β activities were lower 0.66, 0.77, 0.70 times in the head, 0.84, 0.90, 0.90 times in the thorax of Rc, Rd and Rp strains than susceptible strain, but higher 1.19, 1.28, 1.25 times in the abdomen, respectively.

T1 강조 영상에서 선조체 고신호강도를 보이는 당뇨 환자에서의 편측무도증

정승철,이병철,김상윤,배재천,황성희,도화범,박종호 대한신경과학회 1997 대한신경과학회지 Vol.15 No.1

고온 다습한 환경에서의 주관적 착용 쾌적감 평가도구 개발을 위한 기초 연구 : Fuzzy 이론의 적용방법과 요인분석 방법간의 비교 Comparision between the utilization of Fuzzy theory and Factor Analysis

김정화,조승식 한국의류학회 1996 한국의류학회지 Vol.20 No.2

Recently, need for the development of the quantification of subjective evaluation is growing for the production of high-touch and high-tech textile products. In this study, Fuzzy theory is utilized for the evaluation of the wear comfort of the various blouses. Result of a new evaluation method and factor scores, validity of the new evalution technique adopted fuzzy theory was crosschecked with the results of fator analysis and factor scores. As results, fuzzy theory was proved to be adequate methodology to objectify the subjective evaluation of the adequacy of clothing which is worn. When DUNCAN'S multiple comparion among median of the fuzzy composite score were compared with the results of factor score, the sensitivity of the test methods tends to increase. Therefore, it is suggested that fuzzy weighted checklist is an alternative evaluation scale for the subjective comparison of the textile products. In addition, individual median of fuzzy composite score value should be treated by statistical for the sensitive analysis of subjective evalution.