http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
전정례,박정륭,김진,윤시혜 동아시아식생활학회 1995 동아시아식생활학회지 Vol.5 No.2
식물성 단백질 자원으로 참깨의 유지 추출 후 분리되는 박은 단백질 함량이 높으며 질 또한 우수하여 단백질 자원으로서 이용 가치가 높이 평가되고 있으나 종실박에 함유된 oxalate와 phytate는 무기질과 결합하여 단백질의 이용과 기능성을 저해하고 페놀 화합물은 유지 추출 과정에서 높은 열 처리로 인해 변색을 유발아여 참깨박의 이용을 비료나 동물 사료로 제한하고 있다. 따라서 본 연구는 참깨박에 존재하는 비 영양성분을 제거시킬 목적으로 한외여과에 의해 농축 단백질을 제조하여 이들의 항영양 성분과 기타 성분을 기존의 산침전 분리단백질과 비교 검토하여 다음과 같은 결과를 얻었다. 산침전 참깨박 분리단백질의 조단백질 함량은 88.7%로서 18.32%의 단백질이 회수되었으나 10K, 30K, 100K 막을 사용하여 한외여과에 의해 제조된 참깨박 농축단백질들의 조단백질의 함량은 각각 84.2%, 82.7%와 76.4%로 단백질 회수율은 36.44%, 34.69%, 31.43%로 각각 나타났다. 참깨박의 알칼리 용액으로 추출한 다음 한외여과에 의한 단백질의 농축은 oxalate와 phytate의 함량을 상당량 감소시켰으며 분획 분자량 100K의 막을 사용한 한외여과의 경우 oxalate는 85%, phytate는 94%까지 제거되었으나, 페놀화합물의 함량은 산 침전 및 한외여과 공정에 의해 감소되지 않았다. 참깨박의 칼슘과 아연의 칼슘은 한외여과 공정에 의해 감소되었으며 특히 칼슘은 한외여과에 의해 99%나 제거되었다. 한외여과에 의해 제조된 참깨박 농축단백질의 필수아미노산 함량은 분리단백질에 비해 약간 감소되었다. Defatted sesame flour is the by-products obtained after oil extracting process. Although this flour has high quality and quantity of protein, its use is limited only for animal feed and fertilization. Sesame seeds contain antinutrients such as oxalate, phytate and phenol compounds and these compounds lower their nutritive value. Recently, ultrafiltration(UF) has been used to concentrate protein from various food sources. This study was carried out to examine the effects of UF with different membrane pore size on the components of sesame protein concentrates including antinutrients and to compare with that of conventional acid-precipitated sesame protein isolate. The protein contents of sesame protein concentrates prepared by UF using 10K, 30K, 100K were 84.2%, 82.7%, 76.4% and the protein yields were 36.44%, 34.69, 31.43% on the basis of defatted sesame flour, respectivily. Whereas sesame protein isolate was recovered 18.3% and the protein contents was 88.7%. Alkali extraction process at pH 9.0 followed by UF technique reduced oxalate and phytate content. There were 85% and 94% reduction of oxalate and phytate content by UF with membrane pore size of 100K daltons, respectively. However, the content of total phenol compounds was not reduced by this method. About 99% of calcium and 50% of zinc were removed by UF with membrane of 100K daltons. Total essential amino acid contents of sesame protein concentrates prepared by UF were decreased slightly when compared with acid-precipitated sesame protein concentrate.
Bacillus subtilis SS103으로 제조한 된장의 품질 특성 및 Angiotensin 전환효소 저해 활성
홍지수,박정륭,전정례,차명화,김진,김정희 동아시아식생활학회 2004 동아시아식생활학회지 Vol.14 No.4
Three different doengjang(fermented soybean paste) were prepared by using B. subtilis SS103 with high angiotensin converting enzyme(ACE) inhibitory activity, Asp. oryzae and mixed culture of two organisms, respectively. Quality characteristics and the inhibitory activity were compared. β-Amylase activity rapidly increased during 30 days of fermentation, but lower activity was found in doenjang prepared with B. subtilis. However, α-Galactosidase activity decreased for 15 days of fermentation and little activity was observed after that fermentation period. Protease production tended to increase during the fermentation. High protease activity was found only in doengjang prepared with Asp. oryzae, but the doengjang prepared with B. subtilis SS103 showed very low activity. Total isoflavone content at 90 day fermentation was the highest in doengjang prepared with mixture of Asp. oryzae and B. subtilis. ACE inhibitory activity increased during the fermentation period. The highest activity was found in doengjang made with mixture of Asp. oryzae and B. subtilis. Sensory evaluation on doengjang itself and its soup indicated that doengjang made with mixture of Asp. oryzae and B. subtilis showed higher acceptability on taste, flavor and color than those prepared with only Asp. oryzae or B. subtilis, respectively.
김진아,박정륭,차명화,김진,전정례 동아시아식생활학회 1996 동아시아식생활학회지 Vol.6 No.3
Addition of 0.5, 1.0 and 2.0g of succinic anhydride to 2g of sesame protein concentrates succinylated 44.9, 70.0 and 83.1% of the available amino groups, respectively. Considerable amount of phytate were removed in all sesame protein concentrates and the highest reduction was obtained by addition of 2.0g of succinic anhydride. Among the minerals investigated, high amount of calcium and magnesium were presented in defatted sesame flour. In the case of calcium, magnesium and iron, the contents were decreased as the degree of succinylation was increased. Most amino acid content of sesame protein concentrates was not changed by succinylation but lysine was slightly decreased. Result of color measurement showed that the higher degree of succinylation, the higher values of L and b were founded.
Jeong-Ryae Jeon,Joon-Hyuk Choi 한국식품과학회 2007 Food Science and Biotechnology Vol.16 No.6
The protective effect of the water extract of Fraxinus rhynchophylla leaves (FLE) was determined using an animal model of acetaminophen (AAP)-induced nephrotoxicity. The BALB/c male mice used in this study were divided into 3 groups; the normal, AAP-administered, and FLE-pretreated AAP groups. A single dose of AAP induced necrosis of renal tubules and congestion along with edema to a remarkable degree as observed by hematoxylin and eosin stain, and also increased the numbers of terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL)-positive renal tubular epithelial cells. Blood urea nitrogen and plasma creatinine levels were determined to be significantly higher in the AAP group than in the normal group. However, FLE pretreatment resulted in an attenuation of renal tubule necrosis. Regeneration and dilatation of renal tubules were noted, and the numbers of TUNEL-positive cells were reduced in the FLE-pretreated groups. In an effort to detect the bioactive compounds exerting protective effects in FLE, the analysis of phenolic compounds via gas chromatography/mass spectrometry (GC/MS) were performed, and identified esculetin and esculin. The present study indicates that these compounds may exert a protective effect against AAP-induced nephrotoxicity.
Jeong Ryae Jeon,최준혁 한국식품영양과학회 2010 Journal of medicinal food Vol.13 No.4
To develop a functional food from the dietary fiber fraction of germinated barley (Hordeum vulgare L.) (GBF), lactic acid fermentation was attempted using Lactobacillus acidophilus, Streptococcus thermophilus, and Bifidobacterium bifidus. The quality characteristics of the lactic acid-fermented product and its effect on gastrointestinal function in an animal model were examined. The anaerobic fermentation of 1% and 2% GBF yielded lactic acid bacteria at 8.9±1.0×108 and 1.6±0.2×109 colony-forming units/mL, and it was considered acceptable for consumption by sensory assessment. To determine the effect on gastrointestinal function, Sprague-Dawley rats were fed with three types of diets: a normal chow diet and chow diets supplemented with 10% lactic acid bacteria or a yogurt fermented with 2% GBF (GBFY). The rats fed GBFY for 6 weeks gained less body weight, excreted more fecal mass, and had improved gastrointestinal transit as examined with barium sulfate. The effect of GBFY on colonic epithelial proliferation was investigated through loperamide (LPM)-induced constipation in rats. The rats fed with GBFY for 6 weeks were intraperitoneally administered LPM twice daily for 7 days. GBFY supplementation decreased fecal excretion and moisture content in feces and depleted goblet cells as observed by hematoxylin and eosin stain. However, the rats supplemented with GBFY prior to the LPM administration had enhanced bowel movement, mucin secretion, and production of short-chain fatty acids compared with values for the LPM-alone group. Immunohistochemistry revealed that the GBFY supplement increased the numbers of nuclei stained positively for Ki-67 and extended from the base to the middle zone of crypts. These results indicate that GBFY alleviates constipation via the proliferation of the colonic crypts in LPM-administered rats.