백서 심근경색모델에서 시간경과와 경색의 크기에 따른 심자도의 변화

김미성(Mi Sung Kim),박영선(Young Sun Park),권순길(Sun Gil Kwon),지정훈(Jeong Hoon Ji),신종성(Jong Sung Shin),오광식(Kwang Sik Oh),양용모(Yong Mo Yang),연태진(Tae Jin Youn),김동운(Dong Woon Kim),조명찬(Myeong Chan Cho),이용호(Yong Ho 대한내과학회 2002 대한내과학회지 Vol.62 No.1

N/A Background: Magnetocardiogram (MCG), which records the changes of magnetic fields generated by the heart`s electrical activity, theoritically can provide unique data for clinical application. To date, MCG has been investigated only at a single time point after myocardial infarction (MI) with severe left ventricular dysfunction in rats. The purpose of the present study was to investigate sequential changes of MCG after MI and to evaluate effects of infarct size on MCG. Methods: Acute MI were induced by the permanent ligation of left coronary artery in 22 rats. Magnetic fields were recorded just above a rat with Nb Superconducting Quantum Interference Device (SQUID) gradiometer inside a magnetically shielded room. MCG was measured before and immediately after surgery and it was subsequently recorded at the time points of 1, 4 and 6 hours post operatively. MCG was also measured at 1, 3, 7 and 21 days after surgery. Results: Elevation of ST segment and appearance of pathological Q wave on the MCG were evident immediately after the ligation of coronary artery and persisted to 6 hours after MI. On MCG, ST segment was depressed and T wave was inverted from 1 day after MI. In rats with small-and moderate- sized MI (infarct size〈30%), ST depression returned to near the isoelectric level and Q wave disappeared from 7 days after MI. However, ST depression and Q wave were still present in rats with larger infarct (infarct size≥30%). Conclusion: Evolutional changes of MCG were well-recognized up to 21 days after MI. Furthermore, the infarct size can be expressed by the extent of Q wave and ST segment depression on MCG. Taken together, these data indicate that MCG is a helpful modality for the diagnosis, evaluation of infarct size and follow up after MI.(Korean J Med 62:42-48, 2002)

Effects of sperm insemination on the final meiotic maturation of mouse oocytes arrested at metaphase I after in vitro maturation

Yoon, Jeong,Juhn, Kyoung-Mi,Yoon, San-Hyun,Ko, Yong,Lim, Jin-Ho The Korean Society for Reproductive Medicine 2017 Clinical and Experimental Reproductive Medicine Vol.44 No.1

Objective: The aims of this study were to investigate whether fertilization could induce the resumption of meiosis in mouse oocytes arrested at metaphase I (MI) after in vitro maturation (IVM), and to investigate the effect of $Ca^{2+}$ chelator treatment at the time of fertilization on the transition from MI to metaphase II (MII). Methods: MII-stage and arrested MI-stage mouse oocytes after IVM were fertilized, and then embryonic development was monitored. Blastocysts from each group were transferred into 2.5 days post-coitum pseudo-pregnant ICR mice. MI oocytes after IVM were treated with a $Ca^{2+}$ chelator to investigate the effect of $Ca^{2+}$ oscillations on their maturation. Results: As insemination time increased, the number of oocytes in the MI group that reached the MII stage also increased. The blastocyst rates and total cell numbers in the MII group were significantly higher than in the MI group. No pregnancy occurred in the MI group, but 10 pregnancies were achieved (10 of 12) in the MII group. The proportion of MI oocytes that matured to MII oocytes after fertilization was significantly higher in the non-treated group than in the $Ca^{2+}$ chelator-treated group. Conclusion: The findings that a higher proportion of MI-arrested oocytes progressed to MII after fertilization and that the MI-to-MII transition was blocked by $Ca^{2+}$ chelator treatments before fertilization indicate that the maturation of MI oocytes to MII oocytes is associated with intracellular $Ca^{2+}$ oscillations driven by fertilization.

심근경색 모델 흰쥐에서 스트레스가 B-type Natriuretic Peptide 발현 및 심박변이율 변화에 미치는 영향

이삼윤(Sam Youn Lee),이미경(Mi Kyoung Lee),김남호(Nam-ho Kim),주민철(Min Cheol Joo),조항정(Hyang Jeong Jo),강지숙(Ji Sook Kang),김병숙(Byung Sook Kim),윤선식(Sun Sik Yoon),최을식(Eul Sig Choi),이문영(Moon Young Lee) 대한스트레스학회 2010 스트레스硏究 Vol.18 No.3

심박변이율의 감소는 심근 경색 환자에서 예후의 악화와 관련되어 있다. 심박변이율 중 very low frequency가 심부전환자의 예후를 판단할 수 있는 독립적 인자로서 사용이 가능하다고 보고된 바 있으며, 심실의 압력 증가에 반응해서 생성되는 B-type natriuretic peptide가 심근 경색의 예후 인자로서 사용할 수 있음 역시 보고된 바 있다. 본 연구에서는 심근경색 모델을 제작하여 일정의 회복 기간을 거친 다음 다시 일정 기간의 스트레스를 겪게 한 후 심박변이율의 변화 및 심실 조직에서의 BNP 발현 정도를 비교하여 스트레스에 의한 영향을 관찰하고자 하였다. Sprague-Dawley계 수컷 흰쥐 15마리를 대상으로 하였다. 실험동물은 인위적 스트레스 및 수술을 받지 않은 대조군(CON, n=5), 심근경색 수술을 받은 후 restraint stress를 받지 않은 군(MI+No Stress, n=5), 심근경색 수술을 받은 후 1주일 동안 restraint stress를 받은 군(MI+Stress, n=5)으로 분류하였다. 심근경색 모델 제작 직후 15분 이상 심전도의 변화를 기록하였으며, 2개월 정도 후 심근경색 수술을 받은 동물을 두 군으로 나누어 그 중 한 군에는 1주일 동안 restraint stress를 가한 후 restraint stress를 가하지 않은 동물과 심박변이율을 비교 분석하였다. 심실 조직은 좌심실 전벽 부위의 위축을 관찰할 수 있었고, 대식세포에 의하여 응고, 괴사된 심근세포의 탐식과 혈관이 풍부한 육아조직 및 섬유 변화를 볼 수 있었다. 심박변이율은 심근경색 수술을 받은 직후 심박수는 유의한 증가를 보였고, standard deviation of the normal to normal intervals (SDNN), very low frequency (VLF) 및 low frequency (LF)의 유의한 감소를 보였다. 심근경색 수술을 받았던 동물에서 두 달 정도의 회복 기간을 거친 후 스트레스를 받지 않은 군은 심박수 및 기타 심박변이율 분석에서 SDNN 값을 제외하고는 정상군과 유의한 차이를 관찰할 수 없었던 반면 1주일 동안 스트레스를 받은 군에서는 심박수가 다시 유의하게 증가했을 뿐만 아니라 SDNN, VLF 및 LF 역시 정상군과 유의한 차이를 보였다. 이상의 결과를 종합하면 심근경색 동물에 대해 1주일 동안 스트레스를 가한 결과 심박변이율이 감소하고 심실에서의 BNP 발현은 더욱 증가하여 악화함을 보여주고 있다. Diminished heart rate variability (HRV) is associated with less favorable prognosis after myocardial infarction (MI). It has been reported that very low frequency (VLF) power in HRV analysis is an independent risk predictor in patients with congestive heart failure and B-type natriuretic peptide (BNP) can be used as a prognostic factor of MI. The purpose of this study was to investigate whether stress has an effect to the changes of BNP expression and/or heart rate variability in MI model in rats. Male Sprague-Dawley rats were divided into 3 groups: control group (CON), MI group (MI+No Stress), and MI followed by stress group (MI+Stress). MI+Stress group rats were raised for a two month recovery period after the operation, followed by being exposed to restraint stress for 2 hours per day for 1 week. Electrocardiogram was recorded after the operation and the last day after 1 week of stress. The frequency components of HRV were calculated in the frequency domain such as VLF, low frequency (LF), high frequency (HF) and so on. In HRV analysis, standard deviation of the normal to normal intervals (SDNN) was significantly reduced in both groups compared to the control group. VLF and LF also were significantly reduced in MI+Stress group compared to the control groups. In addition, BNP expression in western blotting was shown the strongest bands in MI+ Stress group among experimental groups. These results suggest that BNP and HRV were aggravated by stress in MI rat model. (Korean J Str Res 2010;18:275∼285)

Histone deacetylase 3 is selectively involved in L3MBTL2-mediated transcriptional repression

Yoo, Jung-Yoon,Choi, Kyung-Chul,Kang, HeeBum,Kim, Young Jun,Lee, Jeongmin,Jun, Woo Jin,Kim, Mi-Jeong,Lee, Yoo-Hyun,Lee, Ok-Hee,Yoon, Ho-Geun Elsevier 2010 FEBS letters Vol.584 No.11

<P><B>Abstract</B></P><P>This is the first report that L(3)mbt-like 2 (L3MBTL2) specifically interacts with the histone deacetylase domain of histone deacetylase 3 (HDAC3) via its MBT domain. Here, we show that L3MBTL2 selectively interacts with HDAC3, but not other class I HDACs. An in vitro peptide-binding assay demonstrated the specific association of HDAC3 with methylated histone-K20 tail and L3MBTL2. Furthermore, depletion of HDAC3 resulted in a decrease of methylated K20-H4, as well as an increase in acetylated histone H3. Consequently, HDAC3 knock-down selectively suppressed L3MBTL2-mediated transcriptional repression. Taken together, our results reveal the concerted action of both HDAC3 and L3MBTL2 in histone deacetylation and methylation-dependent transcriptional repression.</P><P><B>Structured summary</B></P><P>MINT-7719975: <I>L3MBTL2</I> (uniprotkb:Q969R5) and <I>HDAC3</I> (uniprotkb:O15379) <I>colocalize</I> (MI:0403) by <I>fluorescence microscopy</I> (MI:0416)</P><P>MINT-7719941, MINT-7719921: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>binds</I> (MI:0407) to <I>HDAC3</I> (uniprotkb:O15379) by <I>pull down</I> (MI:0096)</P><P>MINT-7719991: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-7719958: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>physically interacts</I> (MI:0915) with <I>HDAC3</I> (uniprotkb:O15379) by <I>anti tag coimmunoprecipitation</I> (MI:0007)</P><P>MINT-7719897: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>two hybrid</I> (MI:0018)</P>

소파 방정환에 의해 재창조된 번안동화의 작품세계 -『사랑의 선물』을 중심으로-

김미정 ( Mi Jeong Kim ),임재택 ( Jea Tack Lim ) 한국어린이문학교육학회 2010 어린이문학교육연구 Vol.11 No.2

본 연구의 목적은 방정환이 ‘소년운동’ 차원에서 기획한 번안동화집인 『사랑의 선물』중 임의로 선정한 총 4편을 원작과 비교하여, 원작이 ‘어떻게’, ‘얼마나’, ‘왜’ 다르게 번역되었는지 원작에 대한 번역 일치성 및 개작의 중재 전략을 알아보는 데 있다. 연구 결과 방정환은 번역자로서 내용의 의도적인 첨가, 삭제, 수정의 방법으로 원작을 개작하는 과정을 수행하였다. 또한 이러한 개작은 화합, 자국화, 윤색의 중재 전략이 사용되었는데, 방정환은 이러한 중재전략을 통하여 첫째, 순수한 동심의 세계 존중, 둘째, 외래문화에 대한 주체적 수용, 셋째, 문학적 감성과 상상력을 통한 애국 교육 등의 세계관을 뚜렷이 드러내었다. 이에 본 연구를 통하여 방정환은 『사랑의 선물』번안을 통하여 외국 동화가 문학텍스트로서 가지는 문학적·심리적 가치를 그대로 어린이에게 전달하는 동시에 유교 전통을 극복하고 우리나라 현실에 맞게 아동존중사상을 농축하여 표현한 문화변용의 주체였다는 사실을 알 수 있었다. Bang Jeong-Hwan tried to transfer foreign fairy tales in itself which was worth of literary and psychological value as a literary text to children and simultaneously express realistically condensed ideological contents of DongHak through the adaption of Sa-rang-ui-Suon-mul(A Gift of Love). This study chose four sub-titles of the Sa-rang-ui-Suon-mul to investigate how, how much different, why these translations(adaptions) were different from original on the base of the level of fidelity to original and the intervention strategy. As the results of this study, Bang Jeong-Hwan as a translator performed the process of these translations as a significant difference from original by introducing the method of intensely insertion, elimination, modification. Furthermore, Bang Jeong-Hwan could attain the following three purposes and express a distinguishing his outlook on the world through these adaptations: 1) the respect for the world of pure child mind 2) the creative and independent acceptance of foreign cultures 3) the patriotic education through a literary sensitivity and imagination. As results of this study, we surely knew that Bang Jeong-Hwan was a main agent of acculturation by means of maintaining the literary value of original and realizing an idea of children respect.

Hepatic Cirrhosis Occurring in a Young Woodchuck(Marmota monax) Due to Vertical Transmission of Woodchuck Hepatitis Virus(WHV)

Kyu-shik Jeong,정원일,Jae-yong Chung,Mi-young An,Chae-yong Jung,Gyoung-jae Lee,Jong-soo Kang,Byeong-cheol Kang,Young-heun Jee,Bruce H Williams,Young-oh Kwon,Da-hee Jeong 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.2

Cirrhosis Occurring in a Young Woodchuck (Marmota monax) Due to Vertical Transmission of Woodchuck Hepatitis Virus (WHV)Da-hee Jeong, Won-il Jeong, Jae-yong Chung, Mi-young An, Chae-yong Jung, Gyoung-jae Lee1, Jong-soo Kang1, Byeong-cheol Kang2, Young-heun Jee3, Bruce H Williams4, Young-oh Kwon5 and Kyu-shik Jeong*College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Korea1Shinwon Scientific Co., LTD, Research Institute, Suwon, Korea2Clinical Research Institute, Seoul National University, Seoul 110-744, Korea3College of Veterinary Medicine, Jeju National University, Jeju 690-756, Korea

동진강 수계 농업용수 수질평가

한수곤,최정식,문영훈,엄미정,김갑철 한국환경농학회 2000 한국환경농학회지 Vol.19 No.2

동진강 수계의 수질현황을 파악하기 1994∼1998년 까지 4∼9월에 월 1회에 걸쳐 5개년간 조사한 결과를 요약하면 다음과 같다. 월별로는 빗물에 의한 희석효과로 7, 8월에 수질이 가장 양호하였으며, 4월이 다소 불량하였으나 수도생육 피해 한계농도에는 미치지 못하는 수준이었다. 지점별 수질현황은 생활하수 유입지인 정읍천과 지천의 폭이 좁은 원평천의 무기성분 함량이 높았으며 동진천 상류지역에서 가장 양호하였다. 연도별 현황은 '94년 이후 '95년까지는 수질이 악화되는 경향을 보였으나 '95년 이후로는 각 성분 함량이 낮았지는 등 점점 양호해지고 있으며 COD함량으로 볼 때 특히, 정읍천의 수질이 개선되고 있음을 알 수 있었다. 양이온과 음이온의 총당량온 4월에 가장 높았고 7, 8월에 가장 낮았으며 양이온/음이온의 당량비는 5월에 높았고 7월이 가장 낮았으며 지점에 따라서는 총당량은 정읍천, 원평천, 동진천 하류, 중류, 상류의 순위였으며 양이온/음이온 총당량 비율은 원평천이 상대적으로 작은 수치를 보였지만 지역간 큰 차이는 보이지 않았다. 수질 중 각 성분 상호간의 상관관계를 보면, 대개의 성분 상호간에 유의성이 높게 나타났으나, NO₄^--N는 대개의 성분과 유의성이 없었고, PO₄^(3-)-P도 COD, NH₄^+-N, NO₃^--N, SO₄^(2-)과는 유의성을 보이지 않았다. This study was conducted to monitor the irrigation water qualities along Dong-Jin river watershed. The water quality was surveyed at 6 sites from April to september during 1994∼1998. And the results were as follows : In July and August, water quality was better than that of any other months due to dilution with rainwater. Whereas, it became worse in April but it involved lower contents than limitted contents affected to the crop damage. Content of inorganic components was higher at Jeong-up and Won-pyeong stream. The reason for it that Jeong-up stream was deteriorated with sewage water from Jeong-up city, and Won-pyeong stream has narrow width. Water quality in upstream of Dong-Jin river, was evaluated best conditions in all sampling sites. For investigated period, water quality got worse from 1994 to 1995 but it was getting better to 1998 after 1995, especially at Jeong-up stream. The total equivalent of cation and anion was the highest at April through all months and at Jeong-up stream in sampling sites. Equivalent ratio of cation to anions(∑C/∑A) was higher at May than any other months and lower at Won-pyeong streams than any other sites. The value of most inorganic components was highly correlated with those of other components. But the value of NO₃^--N was not correlated with that of most components, and PO₄^(3-)-P was not correlated with COD, NH₄^+-N, NO₃^--N, SO₄^(2-_.

Factors influencing choosing teaching as a career: South Korean preservice teachers

Jeong‑Ae Lee,Mi Ok Kang,Bitnara Jasmine Park 서울대학교 교육연구소 2019 Asia Pacific Education Review Vol.20 No.3

This study validated the underlying factor structure of the Factors Influencing Teaching (FIT)-Choice survey in the South Korea context and examined motivations and perceptions of 903 Korean preservice teachers by major demographic characteristics. Secondary preservice teachers reported significantly higher values than elementary preservice teachers for Qualification fit and desire to teach and Task return. Male preservice teachers believed more strongly than female preservice teachers that they chose the teaching profession because of Qualification fit, Influencing others, and Task return. Compared to freshmen/sophomores, juniors/seniors reported significantly higher values for Task demand. Participants with low- to medium-level self-reported income ($12K–$46K) weighted Benefits significantly higher than those with medium-level income ($46K–$88K). STEM majors reported higher values for the Qualification fit and Influencing others than non-STEM majors. As South Korea is one of the few countries which has an oversupply of highly qualified teacher candidates, findings were compared with other countries to provide useful insights to improve recruitment of highly qualified individuals to teaching workforce in many countries where teacher shortage is a chronic problem. Based on these data analyses, we argue that policy makers, politicians, education researchers, and others who care about quality education and student academic achievements need to make efforts to increase task returns and benefits.

오정희 소설과 노년 표상의 시점시학

정미숙(Jeong, Mi-suk) 부경대학교 인문사회과학연구소 2013 인문사회과학연구 Vol.14 No.2

오정희는 노년의 다양한 층위를 표상하기 위해 시점 전략을 구사한다. 오정희의 대표적인 노년소설은 ‘남성노인’이 시점의 주체가 되어 노년의 무력한 삶을 고백하면서 시작된다. 노인이 시점 주체인 고백적 화자는 노년의 고독과 소외를 내밀하고 정치하게 들려준다. 노인의 불편한 몸과 정서적 소외가 부각되고 독거노인에 대한 사회적 관심을 야기한다. 여성노인의 경우는 노인과 혈연으로 얽힌 화지(‘손녀’)에 의해 그려지는데, 가족 내에서 의 그녀의 위치와 젠더수행을 중심으로 표상된다. 젠더공간인 가정에서 할머니는 자신의 역할을 헌신적으로 수행한다. 할머니는 실질적인 모성인 ‘씨앗 주머니’로 손녀의 성장과 정체성에 지대한 영향을 미친다. 가족의 구도에서 벗어나 노인을 객관적으로 조명하는 화자에 의해 ‘노인’의 의미가 새롭게 정립된다. ‘노인’은 무력한 몸적 존재가 아닌 예지와 경험을 갖춘 정신력의 소유자로 자리매김한다. 노인은 통행하는 화자에게 진정한 삶의 자세를 성찰하는 계기를 마련한다. The purpose of this study is to use the poetics of viewpoint to completely supplement a stratum of old age that is represented in Oh, Jeong-Hee's Novel. For this study, tills researcher focused on an old man who is one of others appeared in that novel. Oh, Jeong-Hee took a viewpoint strategy to represent different stratums of old age. A representative novel that she wrote about the old generation, starts from 'an old man' who confesses how much his old days are powerless as he leads the viewpoint of that novel. He as the speaker has kinship with an old woman as another character. That woman is represented based on her position and roles in her family. The speaker views the woman objectively and sympathize with her beyond the frame of the family, providing reflection on the roles and meaning of old age and her and the attitudes of true life. Leading the viewpoint of the novel, the revealing speaker say privately but clearly about the loneliness and alienation of old years. This has the bodily inability and emotional alienation of old people come into the spotlight. It also make lots of social attention paid to aged persons living in solitude. Meanwhile, the old woman is so different. She plays lots of different roles at home as a gender space. Throu호out the novel, the old woman is accompanied and objectively viewed by the speaker who is not bloodily related with her, in which she reveals her own unique foresight that is in turn noticed and sympathized with by others. This gets us to be aware of 'an old person' existing inside us and have the time to accept that person with 'hospitality'.

모녀 간 친밀성 서사의 젠더 정치 - 오정희와 백수린의 사례를 중심으로

정미선(Jeong, Mi-seon) 전남대학교 한국어문학연구소 2019 전남대학교 한국어문학연구소 학술지 어문논총 Vol.- No.35

여성 간 친밀성의 서사화 유형과 그 담론적 양상에 대해 물을 때, 모녀 관계는 친족의 경계 내부에서 가장 빈번하게 호출되는 문제적 유형이다. 본고는 모녀 관계의 특수성에 대한 젠더 심리학과 페미니스트 사회학의 젠더화된 삶과 동일시 역학에 대한 성찰을 경유하여, 모녀 간 친밀성을 모의하는 오정희와 백수린의 서사적 사례들이 보여주는 젠더 정치적 면모와 차이 지점들을 분석했다. 여기에서 모녀 간 친밀성의 양식은 어머니와 딸 사이의 상상적 동일시의 구도로 표출되며, 주로 오정희의 사례에서 전경화되는 이러한 병리적 동일시는 ‘여성’과 ‘모성’에 대한 문화적 기대에 깃든 젠더화된 담론적 저변을 드러내는 마스터플롯으로서 형상화된다. 또한 백수린의 사례는 이러한 기존의 플롯들에 기반한 모녀 간 친밀성의 전략이 끝내 실패하게 되는 서사적 귀결을 보여줌과 동시에, 동일시의 역학으로 수렴되지 않는 친애의 친밀성 양식을 새롭게 제시함으로써 모녀 간 친밀성의 플롯들을 정치적 계쟁에 부친다. 이는 젠더 정치의 오랜 화두이자 동시대적인 의제로 자리하는 모녀 관계에 있어서, 여성 간 친밀성이 어떻게 새롭게 상상될 수 있는가에 대한 질문과 함께 대안적 친밀성의 가능성 지대에 대한 탐색을 요청한다. This paper focuses on the very problematic type of ‘mother-daughter relationship’ in terms of ‘female-female kinship’s narrative‘. Psychological and psychological perspectives on gender provide insights into the mother-daughter relationship through studies of gendered lives and identification dynamics―and mechanisms and differentiation patterns such as incorporation, projection, introjection, sympathy, empathy, etc―. This paper analyzes two narrative cases of Oh Jeong-hee and Baek Su-rin through the perspective of ‘kinship"s narrative’ considering the specificity of mother-daughter relationship. The key point of this paper is to discuss the differences in gender and narrative aspects that can be observed in these two cases. According to the results, the pattern of mother-daughter kinship is expressed in the ‘imaginary identification’ composition between mother and daughter. This pathological identification, foregrounded in first novels, is described as a master-plot of the discourse underlying the cultural expectations of the gendered female subject. On the other hand, second novel shows the ending that the strategy of making the kinship of mother-daughter relations based on these plots will eventually fail, and also presents a new form of kinship that does not converge or reduce with the dynamics of identification. Through this the plots of kinship between mothers and daughters are in political litige. This paper reemphasizes gender politics and contemporary aspects of the mother-daughter relationship, and asks how ‘female-female kinship’ can be reimagined. And a search for the spatial possibilities of alternative intimacy is called for.