Pulmonary inflammation caused by silica dioxide nanoparticles in mice via TXNIP/NLRP3 signaling pathway

Je‑Oh Lim,Je‑Won Ko,Tae‑Yang Jung,Woong‑Il Kim,So‑Won Pak,In‑Sik Shin,Won‑Kee Yun,Hyoung‑Chin Kim,Jeong‑Doo Heo,Jong‑Choon Kim 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.3

Background Silica dioxide nanoparticles (SiONPs) have been used for various medical applications, including therapeutics and imaging, and the use of SiONPs has increased gradually over the years. However, despite an increase in the use of SiONPs, not much is known about mechanism of action of SiONPs and their pulmonary toxicity. Objective The present study investigated the pulmonary toxicity of SiONPs and explored the underlying mechanism of action, primarily focusing on thioredoxin-interacting protein (TXNIP)/NOD-like receptor pyrin domain-containing 3 (NLRP3) in SiONPs-treated mice. We investigated the toxic effects of SiONPs in the lung of BALB/c mice administered 5, 10, and 20 mg/kg SiONPs for 3 days. Results Exposure to SiONPs markedly increased inflammatory cell counts, including those of neutrophils and macrophages, and levels of inflammatory mediators, such as interleukin (IL)-1β, IL-6, and tumor necrosis factor-α in a dose-dependent manner in the bronchoalveolar lavage fluid. Moreover, the inflammation was verified upon histopathological analysis. In addition, exposure to SiONPs increased the expression of TXNIP in a dose-dependent manner and, in turn, upregulated NLRP3 inflammasome proteins, which subsequently induced IL-1β production. Conclusion Collectively, exposure to SiONPs induced inflammation in the lungs of mice, which resulted in the activation of IL-1β production via the TXNIP-NLRP3 axis. Our results provide useful information on the pulmonary toxicity induced by SiONPs and provide insights into the underlying mechanism of action.

뇌하수체 종양 제거술 시행 후 뒤늦게 발생한 횡문근 융해증과 동반된 중추성 열

장제혁,최규남,김일환,노은지,김윤정,유충헌,고정해,박봉수,김태균,권민정,이순희,박정현 인제대학교 2008 仁濟醫學 Vol.29 No.-

Body temperature is controlled by thermoregulatory center of the hypothalamus. We report a case of 24 years old man with central fever. He was subjected to a partial excision of pituitary tumor compressing optic chiasm four years ago. He has received hormonal therapy for panhypothyroidism after removal of pituitary tumor. And He received gamma knife operation for partially contrast-enhancing masses in suprasellar and both hypothalamic areas that is probably postoperatively remnant or recurrent tumor of pituitary adenoma. One year after gamma knife operation, he presented with a febrile syndrome of unknown origin including rhabdomyolysis. All usual investigations proved negative. We diagnosed him as central fever with rhabdomyolysis. He received medical ICU care with cooling bed, ice pack. And his symptom improved. Postoperative hyperthermia may result following resection of the pituitary tumor. When central fever is suspected taking note of past history, a quick recognition of course of fever can help reduce the using of unnecessary antibiotics and hospital stay.

제어 시스템 설계를 위한 IEC 1131-3 기반의 제어 로직 생성기의 개발

정구,심주현,이제필,이철수 한국공작기계학회 2001 한국공작기계학회 춘계학술대회논문집 Vol.2001 No.-

This paper describes the methodology of an IEC 1131-3-based control logic generator for the control system design and converting algorithm between programmable languages. The proposed control logic generator is generated based on the software model and common element with data type, variables, POUs(program organization unit) and execution control unit commonly used within programmable languages of IEC 1131-3 Standard. The generation method of object file was proposed on five programmable language based on IEC1131-3. The generation method of object file is represented as following; 1) the generation method using conversion algorithm from LD to IL with FBD(function block diagram), 2) the generation method using C code generation algorithm from SFC using the SFC execution sequence with FBD and ST(structured text). The proposed control logic generator was implemented by Visual C++ and MFC on MS-windows NT 4.0

有機溶劑染色에 의한 PET Filament 의 染色性과 物性變化에 관한 硏究

鄭濟石,李相奎 단국대학교 1989 論文集 Vol.23 No.-

Poly(ethylene terephthalate) filament was dyed with C.I. Disperse Orange 3 in DMF, MCB, and PER dyebath at varying temperature in order to examine the dyeing properties related with the changes in the physical properties. The results of experiment were as follows; 1. Amount of dye uptake decreased in order of PER, MCB, and DMF, and diffusion coefficients of dye were increased according to increasing temperature, but amount of dye uptake were deacreased. 2. Shrinkage and degree of swelling in DMF which interacted with the aliphatic residue of PET were shown better than other solvents. 3. Dye exhausion rate increased the linear proportion with the change in the physical properties increased until the equilibrium dye uptake. 4. In isothermalic condition, degree of crystallinity was increased as time pass.

界面活性劑가 有機실리콘 第四級암모늄鹽의 抗菌性에 미치는 影響

鄭濟石,白欽吉 단국대학교 1987 論文集 Vol.21 No.-

After knitted cotton fabric was finished silicone quaternary ammonium salt of antibacterial agent, effects of treatment by nonionic and anionic surface active agent and salt-methanol rinsing on antibacterial properties were investigated. When laundered with anionic surface active agent, cation of quaternary ammonium, antibacterial functional radical from the first laundry was compete masked with anion of surface active agent, and antibacterial properties was decreased extremely after ten times of laundry. But, as a result of milk test, the effect of antibacterial properties was known as positive comparing with unfinished fabric.

발아과정을 통한 3종 식물의 내염성 단백질 합성

정숙주,우제창 木浦大學校基礎科學硏究所 1997 基礎科學硏究誌 Vol.15 No.-

염분을 농도별로 첨가한 배지에서 유채와 무 0.2%때 발아율이 가장 높았다. 염분의 농도별 생장율을 조사한 결과 유채와 무에서 0.4%와 0.6%의 염분이 첨가 되었을 때 생장이 가장 좋았다. 3종 작물을 각기 다른 염분내성 단백질을 생산해내는 것으로 나타나서 유채는 35kD와 26kD의 단백질을 새로 생산해 내었으며 무는 40kD와 16kD 그리고 벼는 0.4%에는 30kD, 고농도에서는 20kD의 단백질을 생산했다. Germination rate of Brassica and Raphanus was observed highest rate at the 0.2% NaCl concentration. Growth rate of Brassica and Raphanus showed maximum value at the 0.4% and 0.6% respectively. Salt resistant protein was biosynthesized with different molecular weight; Brassica synthesized 35kD and 26kD protein, Raphanus with 40kD and 16kD and rice produced 320kD and 20kD protein at the 0.4% and over 0.6% concentration of NaCl respectively.

反復演習 類型의 CAI 프로그램 設計 및 具現

이상정,안종근,송제국 순천향대학교 1994 논문집 Vol.17 No.3

The use uf computer in learning has advantage of providing various function of audio-visual education regardless of places, especially it enables us to overcome the limitation of individual learning in traditional instruction. Today a lot of CAI courseware is developed and used. But most of them are limited on only one unit or one subject. Because of their lack of sequence in learning, there are problems of systematic learning and connection among grades. The purpose of this study is to design and develope a drill & practice CAI courseware which is proper to realize sequence among grade and easy to feedback. The results of this study are as follows: 1. After choosing arithmetics of the 5th and 6th grade of elementary school, it is designed and developed a 32 sub-subjects CAI program according to the 17 subjects in 3 units. 2. This program is implemented menu-driven meihod in order to learn total arithmetics learning. It will be completed by a following study or research for unimplemented parts.

인라인스케이트 트레이닝이 체력과 등속성 근력에 미치는 영향

김재윤,양정옥 신라대학교 자연과학연구소 2006 自然科學論文集 Vol.15 No.-

This study was intended to investigate the effects of inline skate training on fitness and isokinetic in adult males(experiment=7, control=7). For this purpose, the healthy males volunteers having no regular exercise training completed ten-weeks' inline skate training. The results were described as follows; 1) Inline skate training is promote fitness but not significantly. Especially, side-step test is increased (p<.05) significantly. 2) Inline skate training is effected extension strength than flexor strength. Especially, joint angle is increased(p<.05) significantly. In conclusion, it might be thought that inline skate training improved the levels of fitness and isokinetic in adult males. Thus, inline skate thought appropriate exercise program in adults.

차체용 드로우 다이의 블랭크 홀더 굽힘 변형 해석

인정제,신용승,김헌영,김재우,송명환,박진수 한국공작기계학회 2000 한국공작기계학회 추계학술대회논문집 Vol.2000 No.-

The contact forces between die components for the drawing of large size automotive panels introduce elastic deflections of the die components. Due to the deflections, the gap between blank holder and die varies locally resulting in nonuniform material flow. Such a nonuniform die gap usually requires correcting operation, so called die spotting, which is time consuming trial and error process. To reduce the die spotting time, the optimization of the blank holder bending deflection is needed. In this paper, we implemented an analysis procedure to predict the blank holder deflection. The analysis procedure and design of experiments techniques are applied to the optimization of balance block heights. The optimization results can be used as guidelines in actual die spotting process.

計測을 통한 흙막이構造物 安定性 檢討에 關한 硏究

정석영,박춘수,류제천 忠南大學校 産業技術硏究所 1995 산업기술연구논문집 Vol.10 No.2

Recently, many deep excavation have been practiced in constructing building or subways in urban areas to utilize more effectively underground space. In doing such underground excavations, information-oriented construction through the field measuring is getting more important not to give damages the next buildings and to practice in safety. The earth pressure, horizontal displacement, underground water table and so on are included in the item to make sure of safety through the field measuring. The empirical earth pressure come from the strut retention. But in recent days the two reasons have made the construction having been used through anchored retention why the width of earth retaining is so wide and here is a purpose to make use wider and wider the space of work. I come to make conclusions as follow to observe the safety of earth retaining structures with horizontal displacement and underground water table and to study lateral earth pressure of anchored retention earth retaining walls with load cells on the anchored retention sites.