이담자효모 Rhodosporidium toruloides의 막단백질 인산화와 성 Pheromone, Rhodotorucine A의 작용

정영기 동의대학교 기초과학연구소 1998 基礎科學硏究論文集 Vol.8 No.1

When the membrane protein fraction of mating type a cells of heterobasidiomycetous yeast R. toruloides was phosphorylated in vitro, two phosphorylated proteins of 72Kd and 57Kd were detected on SDS-polyacryamide gel. The phosphorylation reaction was inhibited by rhodotorucine A(Rh. A) which is a sexual pheromone secreted by mating type A cells. The inhibition of phosphorylation by Rh.A was dependent on Ca^2+, and independent on Mg^2+ or calmodulin. When adding trigger peptidase (TPase) inhibitor, antipain, no inhibition of phosphorylation was observed. Also, by adding the trypsin-digested product of Rh. A, the phosphorylation was inhibited as the action of Rh. A. From these results, it is expected that the inhibition of membrane protein phosphorylation should be caused by the digested product of Rh. A with TPase.

이담자균 효모 Rhodosporidium toruloides에서 Rhodotorucine A에 의한 막단백질 인산화의 저해와 Trigger Peptidase의 관련성

정영기,이태호,류병호 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.6

이담자 효모균 Rhodosporidium toruloides 접합형 a 세포의 막단백질 가용화 분획을 비변성 전기영동에 의하여 인산화 한 결과 인산화 되는 단백질을 발견하였다. 이 단백질의 인산화는 Rhodotorucine A (Rh. A)에 의하여 저해되었다. 본 인산화 단백질에는 trigger-peptidase (TPase)가 존재하고 있는 것을 확인 하였으며, TPase inhibitor(antipain)를 반응액에 첨가할 경우 Rh. A에 의한 인산화 저해작용은 나타나지 않았다. 그리고, Rh. A를 trypsin으로 분해한 산물을 반응액에 첨가했을 경우 인산화 저해현상이 있는 것으로 보아, 인산화의 저해반응에는 TPase가 중요한 작용을 하는 것이 확인되었다. [γ-^32P]ATP was used to test phosphorylation of membrane proteins of mating type a cells of heterobasidiomycetous yeast Rhodosporidium toruloides separated by non-denaturing electrophoresis. The phosphoprotein was observed in the membrane proteins. The phosphorylation was inhibited by the pheromone rhodotorucine A (Rh. A) secreted by mating type A of the yeast. Rh. A didn't inhibit the phosphorylation in the presence of a trigger peptidase (TPase) inhibitor, antipain. Partially digested Rh. A by trypsin maintained the phosphorylation inhibitory activity. These results show that TPase activity plays an important role in the transduction of pheromone signal in the yeast.

호염세균으로부터 추출한 카로테노이드 색소의 안정성

정영기,최병대 동의대학교 기초과학연구소 2000 基礎科學硏究論文集 Vol.10 No.1

A carotenoid pigment, which was a determinant for food quality, was extracted from a marine halophilic bacteria. The stability of the pigment extract was investigated for a food additive. The optimum temperature for stability was 20℃. The pigment degradation was significantly affected by solvent polarity, however, stable in salvent methanol and ethanol. The pigment degradation was highly sensitive to light and UV exposure.

이담자효모 Rhodosporidium toruloides의 성pheromone(Rh.A)에 의한 성접합형 특이적 인산화 저해 반응

정영기 동의대학교 기초과학연구소 1998 基礎科學硏究論文集 Vol.8 No.1

Two phosphorylated proteins having molecular weights of 57kD and 72kD were detected from the solubilized membrane protein fraction of mating type a cells of Rhodosporidium toruloides which belongs to heterobasidiomycetous yeast. The phosphorylation of the protein was inhibited by a sexual pheromone, Rhodotorucine A (Rh. A), which is secreted from mating type a cells. On the other hand, counterpart mating type A cells and M-39 strain which is a sterile mutant derived from a cells, had also the same two phosphorylated proteins. However, the phosphorylation of the protein from A cells and M-39 strain were not inhibited by the Rh. A. It suggests that inhibition of the phosphorylation reaction by the Rh. A in mating type a cells is a mating-type-specific reaction that relate to transduction mechanism of sexual pheromone signaling.

Van der Waals集團에 대한 電子ion化와 電子부착

鄭圻柱,金容福,李熙福 공주대학교 사범대학 과학교육연구소 1998 과학교육연구 Vol.29 No.1

초음파 확장에 의한 van der Waals집단에 대한 電子想互關係는 二重焦點 質量分析器로 硏究되어 왔다. 특별히 本 硏究에서는 ??집단 이온의 생성과 안정성 그리고 O₂와 CO₂집단에 대한 電子부착을 零에너지에 접근하는 고효율 電子부착기로 실시하였다.

力學과 熱物理에서의 自由度 比較

정기주,김용복 공주대학교 사범대학 과학교육연구소 1999 과학교육연구 Vol.30 No.1

과학교육에 있어서 올바른 개념의 정립은 매우 중요하다. 그런데 고등학교 「물리Ⅱ」 교과서 `에너지와 열' 단원에 나와있는 자유도에 대한 개념 설명이 교과서마다 달라서 이의 정확한 설명이 필요한 실정이다. 그래서 본 연구에서는 역학에서 사용되는 자유도와 열역학에서 사용되는 자유도에 대한 정의를 비교하고, 이를 고등학교 「물리Ⅱ」 교과서 `에너지와 열' 단원에 나와있는 단원자 및 이원자 기체분자에 적용하여 각각의 자유도를 계산하였다. 이를 통하여 자유도에 대한 올바른 개념 형성에 도움이 될 것이라 기대된다. It is very important to establish a correct concept in science education. The explanation on the degree of freedom in the section of thermodynamics of textbooks of high school level is different each other. It is needed to explain about the term in a correct and unified way. We compared definitions for degree of freedom between unit of mechanics and thermodynamics, and apply it to monatomic and diatomic ideal gas molecules used in physics textbook of high school. We hope the result play an important role to construct the concept for degree of freedom in textbooks of high school level.

두개골의 3차원 영상 분석을 위한 전산화단층촬영 방법의 비교 : 상층 두께가 3차원 영상의 계측에 미치는 영향

정호걸,김기덕,박혁,김동욱,정해조,김희중,유선국,김용욱,박창서 대한구강악안면방사선학회 2004 Imaging Science in Dentistry Vol.34 No.3

Purpose : To evaluate the quantitative accuracy of three-dimensional (3D) images by means of comparing distance measurements on the 3D images with direct measurements of dry human skull according to slice thickness and scanning modes. Materials and Mathods : An observer directly measured the distance of 21 line items between 12 orthodontic landmarks on the skull surface using a digital vernier caliper and each was repeated five times. The dry human skull was scanned with a Helical CT with various slice thickness (3, 5, 7 mm) and acquisition modes (Conventional and Helical). The same observer measured corresponding distance of the same items on reconstructed 3D images with the internal program of V-works 4.0 (Cybermed Inc., Seoul, Korea). The quantitative accuracy of distance measurements were statistically evaluated with Wilcoxons’ two-sample test. Results : 11 line items in Conventional 3 mm, 8 in Helical 3mm, 11 in Conventional 5 mm, 10 in Helical 5 mm, 5 in Conventional 7 mm and 9 in Helical 7 mm showed no statistically significant difference. Average difference between direct measurements and measurements on 3D CT images was within 2 mm in 19 line items of Conventional 3 mm, 20 of Helical 3 mm, 15 of Conventional 5 mm, 18 of Helical 5 mm, 11 of Conventional 7mm and 16 of Helical 7 mm. Conclusion : Considering image quality and patient’s exposure time, scanning protocol of Helical 5 mm is recommended for 3D image analysis of the skull in CT.

이담자균 효모의 성분화 과정중 인지질의 작용과 배지조성의 제한이 성분화에 미치는 영향

정영기,강원대,남수완 동의대학교 기초과학연구소 1997 基礎科學硏究論文集 Vol.7 No.1

The action of phospholipid on the rhodotorucine A(Rh.A) acceptance by heterobasidiomyceteous yeast Rhodospori-dium toruloides mating type a cells and the effect of medium composition during sexual differentiation were investigated. Activation of trigger peptidase(TPase) was very sensitive to the originated phospholipid from R. toruloides and was more sensitive to phospholipid liposome made up of phospholipid. Phospholipid present on the membrane of mating type a cells consists of phospatidylglycerol(PG), phosphatidylethanolamine(PE), phospatidylcholine(PC), phospatidylinositol(PI), and phosphatidylserine(PS) of 12.9, suprisingly 45.4, 11.0, and 13.9%, respectively. As the result of using C-1 and N-1 mediums which limited C and N sources capable of inhibiting the synthesis of phospholipid. it resulted inhibiting sexual differentiation and production of Rh.A from mating type A cells.

김치와 김치재료의 콜레스테롤 저하 및 항암효과

정영기,이재준 동의대학교 기초과학연구소 2000 基礎科學硏究論文集 Vol.10 No.1

The purpose of the paper is to explore the current knowledge on the nutritional evaluation, cholesterol-lowering effect and antitumor activity of kimchi and its ingredients(Korea cabbage, garlic, red pepper powder, ginger and onion). Kimchi contains high contents of nutrients such as vitamins(ascorbic acid, β-carotene and vitamin B complex), minerals(calcium, potassium, iron and phosphorous), essential amino acids and dietary fiber. Kimch also contains high levels of lactic acid bacteria, allicin, capsaicin, organic acid, phenol compounds, flavonoid and sulfur compounds. The dietary fiber and lactic acid bacteria isolated from kimchi are effective in improving intestinal microflora of human. Insoluble dietary fiber shows anticancer activity, but soluble dietary fiber shows hypocholesterolemic effect. Lactic acid bacteria isolated from kimchi acts as a hypocholesterolemic or anticancer agent. A major ingredient of kimchi is mainly cruciferous and allium family vegetables, which were also reported to prevent cancer and atherosclerosis. It is suggested that kimchi is important not only as one of traditional fermented Korea foods but also as therapeutic agent for carcinogenesis and hypercholesterolemic state.

1998-1999년 절기에 부산지역에서 유행한 인플루엔자 바이러스의 분리

정영기,정명주,이주연,안정배,김지희,김만수,조경순 동의대학교 기초과학연구소 2000 基礎科學硏究論文集 Vol.10 No.1

Investigate the epidemics for influenza outbreaks. The outbreak pattern of the internal and external patients housed in the 10 designated hospitals was monitered to investigated and the characteristics of the virus isolates are as follows. 232 strains of influenza virus was isolated from the oral specimen of 1,320 respiratory disease patients in Pusan from Oct. 1998 to Jun. 1999. Among these isolates, 222 strains were A-type and the rest were B-type. The outbreak pattern for sex-and age-group is as follows. The male outbreak was similar to the female outbreak: male outbreak, 47.4% and female outbreak, 52.5%. Most of the patients were less than 10 years old. The monthly influenza outbreak was consistent from Dec. 1998 to Apr. 1999. and The 113 strains from the A-type isolates were A/Sydney/05/97(H3N2)-like, the 109 strains were A/Beijing/262/95(H1N1)-like, and all of the 10 B-type isolates were B/Harbin/07/94-like.