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Development of Species Identification Method of Trapped and Damaged Sample using Direct PCR
Kim Ju Il,Kwon Min,Kim Jeom Soon,Lee Yong Gyu,Shim Jae Dong,Kim Seong Hee 한국응용곤충학회 2012 한국응용곤충학회 학술대회논문집 Vol.2012 No.10
Lepidopteran pests monitoring in adult stage was generally performed using delta or corn typed trap including rubber septa impregnated sex pheromone (lure). Sometimes, unfortunately trapped samples were severly damaged because of biotic and/or abiotic environments such as micro-organism, predator and rain, sticky material, respectively. In our case, we monitored potato tuber moth, PTM, Phthorimaea operculella distribution during 2009~2012 in Korea. However, we encountered unexpected problem, another species can be trapped in species specific sex pheromone trap. Therefore, species confirmation was needed in trapped samples. Here we developed confirmation method by direct PCR (without DNA extraction) or sequencing methods which trapped samples that cannot identified by morphologically. We designed multi-plex PCR universal primers and species specific primers in rRNA region because to check the success of PCR and species identification. This direct PCR method can be applied in other species confirmation which monitored using pheromone trap.
A mid-late maturing, multi-disease resistant and good-quality rice variety Samkwangbyeo
Yeon-gyu Kim,Ha-cheol Hong,Hung-goo Hwang,Im-soo Choi,Young-chan Cho,Man-kee Baek,Yong-hwan Choi,Kyung-ho Kang,Young-seop Shin,Jeom-ho Lee,Hong-yeol Kim,Jong-doo Yea,Hae-yeong Ryu,Eung-ki Jeong,Chang- 한국육종학회 2007 한국육종학회지 Vol.39 No.4