Molecular Targeted Therapy for Hepatocellular Carcinoma: Learning from Genome-matched Trials in Other Solid Cancers

( Jeeyun Lee ) 대한간학회 2016 춘·추계 학술대회 (KASL) Vol.2016 No.1

Molecular profiling of actionable mutations in refractory cancer patients has the potential to enable “precision medicine,” inwhich individualized therapies result in improved treatment outcomes. However, its clinical benefit in practice has not been clearlydemonstrated in large cohorts with multiple cancer types.The NEXT (Next generation pErsonalized tX with mulTi-omics and preclinical model) trial is a master protocol to route participantsto different candidate drugs in trials based on clinical sequencing report. In this trial, we used a customized targeted enrichmentpanel consisting of cancer-related genes to interrogate single nucleotide variants (SNVs), insertions and deletions (Indels), copynumber variants (CNVs) and a subset of gene fusions, that were of clinical significance. In this master protocol, the responserate was assessed as the primary end point in patients who had molecularly-matched or standard therapy. Immunohistochemicalstaining was performed on MET, PTEN, EGFR, and HER2. From August 2014 through April 2015, 541 patients consented toparticipate in precision oncology clinic at a single center. Of 541 patients, 94 patients were excluded and 418 cancer patientshad sequencing data available to clinician for guidance to matched trials. The patient cohorts were gastric cancer (N = 127),colorectal cancer (N =122), pancreatic/biliary tract cancer (N=62) and sarcoma and cancer (N = 67). Of 418 patients, 159 (38.0%)patients were not treated beyond standard chemotherapy, 187 (44.7%) patients had at least one genomic variant (N=74, nomatched therapy available) and 60 (14.4%) patients were successfully routed to genome-based matched clinical trial. In thispresentation, matched trials including NEXT-1 and VIKTORY trial with collateral patient derived tumor cell screening programwill be presented.

한국어 듣기 평가에서 영상 자료의 역할 -숙달도에 따른 차이를 중심으로-

심지연 ( Jeeyun Sim ) 이중언어학회 2019 이중언어학 Vol.75 No.-

The purpose of this study is to confirm the roles of video in listening test and to measure visual factors influencing listening comprehension by its degree of proficiency. This study conducted two different types of tests: audio only test and audiovisual test were performed on 31 learners. The result showed that learners in audiovisual test had performed better in Korean listening comprehension assessment and it had statistically meaningful difference. As a result of investigation on visual factors which are helpful in listening understanding, it was verified that situation and psychological factors ranked high, but speaker related factors were verified in low ranking. More Specifically, it was found that both the listening situation and the learner’s psychological status were helpful for listening comprehension, whereas mouth shape, facial expression and gesture had limited impact. Based on these results, the concept of Korean listening ability was broadly defined including ability to use visual cues throughout this thesis. (Korea University)

검색 엔진의 공신력과 정보의 순위가 정보의 검색과 활용에 미치는 영향

오지연(Jeeyun Oh),박승관(Sung Gwan Park) 한국언론학회 2009 한국언론학보 Vol.53 No.6

Identification of <i>ROS1</i> rearrangement in gastric adenocarcinoma

Lee, Jeeyun,Lee, Seung Eun,Kang, So Young,Do, In‐,Gu,Lee, Sujin,Ha, Sang Yun,Cho, Jeonghee,Kang, Won Ki,Jang, Jiryeon,Ou, Sai‐,Hong Ignatius,Kim, Kyoung‐,Mee Wiley Subscription Services, Inc., A Wiley Company 2013 Cancer Vol.119 No.9

<P><B>Abstract</B></P><P><B>BACKGROUND:</B></P><P>Recently, chromosomal rearrangements involving receptor tyrosine kinases (RTKs) have been described in common epithelial malignancies, including nonsmall cell lung cancer (NSCLC), colorectal cancer, and breast cancer. One of these RTKs, c‐ros oncogene 1, receptor tyrosine kinase (ROS1), has been identified as a driver mutation in NSCLC, because its inhibition by crizotinib, an anaplastic lymphoma receptor tyrosine kinase (ALK)/met proto‐oncogene hepatocyte growth factor receptor (MET)/ROS1 inhibitor, led to significant tumor shrinkage in <I>ROS1</I>‐rearranged NSCLC. Currently, only human epidermal growth factor 2 (HER2)‐targeted therapy in combination with chemotherapy has been successful in significantly prolonging the survival of patients with advanced gastric cancer (GC). There is a need for the discovery of additional novel targets in GC.</P><P><B>METHODS:</B></P><P>Anti‐ROS1 immunohistochemistry (IHC) was used to screen 495 GC samples and was followed by simultaneous <I>ROS1</I> break‐apart fluorescence in situ hybridization (FISH) and reverse transcriptase‐polymerase chain reaction (RT‐PCR) analyses in IHC‐positive samples. Fusion partners in <I>ROS1</I>‐rearranged GC were determined by RT‐PCR. In all 495 samples, <I>HER2</I> amplification was identified with FISH, and MET expression was identified by IHC.</P><P><B>RESULTS:</B></P><P>Twenty‐three tumor samples were ROS1 IHC‐positive. Three of 23 patients were <I>ROS1</I> FISH positive, <I>HER2</I> FISH negative, and negative for MET overexpression; and 2 of those 3 patients harbored a solute carrier family 34 (sodium phosphate), member 2 (<I>SLC34A2)</I>‐<I>ROS1</I> fusion transcripts. No fusion partner was identified in the third patient. Both patients who had <I>SLC34A2</I>‐<I>ROS1</I> transcripts had poorly differentiated histology with recurrence and death within 2 years of curative surgery. ROS1 IHC‐positive status was not identified as an independent prognostic factor for overall survival.</P><P><B>CONCLUSIONS:</B></P><P>In this study, an <I>SLC34A2</I>‐<I>ROS1</I> rearrangement was identified in GC, and the results provide a rationale for investigating the clinical efficacy of ROS1 inhibitors in this unique molecular subset of GC. Cancer 2013. © 2013 American Cancer Society.</P>

Pharmacokinetics of Intravitreally Injected Bevacizumab in Vitrectomized Eyes

Ahn, Jeeyun,Kim, Hyuncheol,Woo, Se Joon,Park, Ji Hyun,Park, Sunyoung,Hwang, Duck Jin,Park, Kyu Hyung Mary Ann Liebert 2013 Journal of ocular pharmacology and therapeutics Vol.29 No.7

대학생이 인식하는 “수업 중 교수-학습 활동 종류와 실행도 및 효과”에 관한 연구

장지윤(Jeeyun Chang),김주영(Juyoung Kim),박인우(Innwoo Park) 한국교육방법학회 2017 교육방법연구 Vol.29 No.2

본 연구는 실제 대학수업에서 이루어지고 있는 교수-학습 활동의 종류와 실행도를 파악하고, 학습자 들이 실제 학습에 효과적이라고 인식하는 교수-학습 활동에 대해 조사함으로써 대학교육에서의 교수·학습의 질 향상을 위한 기초자료를 마련하기 위해 실시하였다. 이를 위해 국내·외 문헌 및 선행연구와 전문가 검토를 통해 ‘교수법 유형에 따른 교수-학습 활동 목록’을 도출하여 대학에서 ‘수업중 활동의 종류와 실행도 및 효과’에 관한 설문지를 제작·개발하였다. A 대학교 재학생들을 대상으로 설문조사를 하고, 각 문항별 실행도와 평균을 통해 중심경향성을 분석하여 실제 대학수업에서 하는 교수-학습 활동의 종류와 학습의 효과성을 파악하였다. 또한 교수-학습 활동의 실행도와 학습의 효과성과의 관계 및 의미를 분석하기 위해 중요도-실행도 분석(IPA)을 활용하였다. 연구결과, 실제 대학수업에서는 주로 설명식 수업, 발견학습, 문제기반학습, 협동학습, 토의식 수업, 모의실험 등과 같은 교수법을 활용한 수업에서 교수-학습 활동이 이루어지고 있는 것으로 확인되었다. 또한, 대학생들이 실제로 학습에 효과적이라고 인식하는 교수-학습 활동은 상위 6개 모두 설명식 수업 중에 이루어진 것으로 나타났다. 마지막으로, 대학수업에서 교수-학습 활동의 실행도와 효과성을 함께 비교·분 석하여 대학교육에서의 교수·학습의 질 제고를 위한 시사점을 제시하였다. This study is identification of type, performance and effectiveness of the teaching-learning activities being carried out in a university class. And then, it subsequently provides preliminary data for improving the quality of teaching and learning in university education by inquiring the teaching-learning activities. Undergraduate students realize their activities to be effective for the actual learning. We classified the list of teaching-learning activities by types of teaching methods through related literature and specialists’ review. With the result, we then developed the questionnaire on ‘type, performance and effectiveness of teaching-learning activities in university class’. We used by Importance-Performance Analysis(IPA) to analyze the relationship and meaning between performance of teaching-learning activities and the effectiveness of learning. Teaching-learning activities such as ‘explanatory lecture, discovery learning, problem-based learning, collaborative learning, discussion, simulation’ were to be used frequently. In addition, the top 6 teaching-learning activities that undergraduate students perceive to be effective for learning actually appeared during the explanatory instruction. Based on these results, we suggested some implications for improving the quality of teaching-learning in university education by comparing and analyzing the relationship of the performance and effectiveness of teaching-learning activities in the university class.

Phase I Trial of Anti-MET Monoclonal Antibody in MET-Overexpressed Refractory Cancer

Lee, Jeeyun,Kim, Seung Tae,Park, Sungju,Lee, Sujin,Park, Se Hoon,Park, Joon Oh,Lim, Ho Yeong,Ahn, Hongmo,Bok, Haesook,Kim, Kyoung-Mee,Ahn, Myung Ju,Kang, Won Ki,Park, Young Suk Elsevier 2018 Clinical colorectal cancer Vol.17 No.2

<P><B>Abstract</B></P> <P><B>Background</B></P> <P>Samsung Advance Institute of Technology-301 (SAIT301) is a human immunoglobulin G2 antibody that can specifically target mesenchymal epithelial transition factor (c-MET). This novel antibody has higher priority over hepatocyte growth factors when binding to the Sema domain of c-MET and accelerates the internalization and degradation of c-MET, proving its powerful antitumor activities in intra- as well as extracellular areas.</P> <P><B>Materials and Methods</B></P> <P>SAIT301 was administered intravenously once every 3 weeks in c-MET overexpressed solid tumor patients, focusing on metastatic colorectal cancer (CRC) according to common clinical phase I criteria. Dose escalation was performed according to a modified Fibonacci design, following the conventional 3+3 design. The purpose of this phase I study was to assess the safety profile, to establish the recommended dose for clinical phase II studies and to assess potential anticancer activity of the compound.</P> <P><B>Results</B></P> <P>Sixteen patients with a median age of 56 (range, 39-69) years were enrolled in the study. The most common adverse events were decreased appetite (50.0%), hypophosphatemia, fatigue and dizziness (25.0%, respectively), and diarrhea, blood alkaline phosphatase increased and dyspnea (18.8%, respectively). For tumor response, no patients achieved complete response. One (9.1%) CRC patient had a partial response in the 1.23 mg/kg group, 4 (36.4%) patients achieved stable disease (2 in the 0.41 mg/kg group, 2 in the 1.23 mg/kg group, 0 in the 3.69 mg/kg group, and 1 in the 8.61 mg/kg group). Because of the increase in dose-limiting toxicities (DLTs) at 8.61 mg/kg, the 3.69 mg/kg dose was considered the maximum tolerated dose and selected for further assessment in phase II.</P> <P><B>Conclusion</B></P> <P>We successfully completed a phase I trial with MET antibody in a MET-overexpressed patient population focusing on CRC, and found that the DLTs were alkaline phosphatase elevation or hypophosphatemia. The recommended dose of SAIT301 for phase II is the dose of 3.69 mg/kg.</P>

교사의 수업방식과 숙제 피드백이 학생의 수업태도, 수업 분위기, 만족도, 자기주도학습, 학업성취도에 미치는 영향: 국어, 수학, 영어 교과 중심으로

장지윤 ( Chang Jeeyun ),박인우 ( Park Innwoo ),김은진 ( Kim Eunjin ) 부산대학교 과학교육연구소 2018 교사교육연구 Vol.57 No.2

본 연구는 주요교과인 국어, 수학, 영어를 중심으로 교사의 수업방식과 숙제 피드백이 학생의 수업태도, 수업분위기, 만족도, 자기주도학습, 학업성취도에 미치는 영향을 탐구하였다. 이를 통해 수업에서 교수 변인이 학습자에게 어떠한 영향을 주는지를 살펴봄으로써 교사의 수업전문성 신장과 학교 현장의 수업 개선에 기여하고자 하였다. 기존의 선행연구들을 분석하여 교수변인으로는 수업방식, 피드백을, 학습자 변인으로는 수업태도, 수업분위기, 만족도, 자기주도학습, 학업성취도를 선정하고, 이 변인들간의 관계 및 효과를 구조방정식 모델을 활용하여 분석하였다. 본 연구에서는 서울교육연구정보원이 2015년에 수집한 제6차년도 서울교육 종단연구(SELS) 자료 중 235개 일반계 고등학교의 3학년 학생 2,907명과 해당 학생들을 가르치는 국어교사 632명, 수학교사 648명, 영어교사 658명의 설문자료를 사용하였다. 연구결과, 교사의 수업방식은 학생의 수업태도, 수업분위기, 수업만족도, 자기주도학습에는 영향을 주는 것으로 나타났으며, 특히 수학교과와 영어교과의 경우에는 학업성취도에도 영향을 미치는 것을 확인하였다. 또한 교사의 숙제 피드백은 국어교과의 수업태도, 수업분위기 및 학업성취도에 영향을 미쳤으며, 영어교과의 학업성취도에 유의미한 영향을 주는 것으로 나타났다. 이와 같이 교사의 수업방식과 숙제피드백이 학습자에게 미치는 영향을 고려할 때, 수업에서 교사는 학생 참여 중심의 수업을 설계·실행함으로써 교사-학생간의 원할한 상호작용과 자율적인 수업분위기를 조성할 필요가 있음을 시사한다. The purpose of this study was to investigate the effect of teachers’ teaching method and homework feedback on students’ class attitude, classroom atmosphere, class satisfaction, self-directed learning, and academic achievement in the core curriculum classes (Korean, Mathematics, English). This study confirmed that teacher’s teaching method and feedback on homework has an influence student’s learning. This search for relationships and effects between these variables was done by utilizing the structural equation model and tried to provide positive support to the teacher’s professionalism. The research data were collected from the Seoul Educational Research and Information Institute, using panel data from 6th year (2015). The participants of this research were 2,907 3rd grade high school students and 1,938 general high school teachers. As a result of the study, it was found that the teacher’s teaching method influenced the student’s attitude of class, classroom atmosphere, class satisfaction, and self-directed learning. Especially, mathematics and English classes had an significant effect on academic achievement. In addition, the homework feedback from the teacher influenced the class attitude, the class atmosphere and the academic achievement of the Korean class and had a significant effect on the academic achievement of the English class. Considering the influence of teacher’s teaching method and homework feedback on learners, teachers in the class suggest that it is necessary to create a smooth interaction between the teacher and the students and an atmosphere of autonomous class by designing and executing the student’s participating classes.

Comparison of Optical Coherence Tomography Biomarkers between Bevacizumab Good Responders and Nonresponders Who were Switched to Dexamethasone Implant in Diabetic Macular Edema

Jeong Hyun Lee(Jeong Hyun Lee ),Joo Young Shin(Joo Young Shin),Jeeyun Ahn(Jeeyun Ahn) 대한안과학회 2023 Korean Journal of Ophthalmology Vol.37 No.2

Purpose: To compare volumetric optical coherence tomography (OCT) biomarkers in bevacizumab responsive and bevacizumabrefractory diabetic macular edema (DME) patients switched to the dexamethasone implant to ultimately identify possibleprognostic indicators. Methods: Retrospective analysis of DME patients treated with bevacizumab were done. Patients were divided into thosewho showed response to bevacizumab (bevacizumab only group) and others who were switched to the dexamethasone implantdue to lack of response to bevacizumab (switching group). Volumetric OCT biomarkers such as central macular thickness(CMT), inner and outer cystoid macular edema (CME) volume, serous retinal detachment (SRD) volume, retinal volume (CME+ SRD volume) within the 6-mm Early Treatment of Diabetic Retinopathy Study circle were calculated. OCT biomarkers werefollowed up throughout treatment. Results: Among total of 144 eyes, 113 patients were included in the bevacizumab only group and 31 patients were includedin the switching group. Compared to the bevacizumab only group, the switching group showed higher baseline CMT (558.00± 209.60 μm vs. 454.96 ± 125.88 μm, p = 0.003), larger inner CME (6.02 ± 1.43 mm3 vs. 5.12 ± 0.87 mm3, p = 0.004) and SRDvolume (0.32 ± 0.40 mm3 vs. 0.11 ± 0.09 mm3, p = 0.015) and higher proportion of patients with SRD (58.06% vs. 31.86%, p =0.008). In the switching group, CMT, inner CME and SRD volume all showed significant reduction after switching to the dexamethasoneimplant. Conclusions: DME with large SRD and inner nuclear layer edema volume may be more effectively treated with the dexamethasoneimplant than bevacizumab.

Assessment of Risk Factors Affecting Refractive Outcomes after Phacovitrectomy for Epiretinal Membrane

Yu Jin Roh(Yu Jin Roh),Joo Young Shin(Joo Young Shin),Tae Wan Kim(Tae Wan Kim),Jeeyun Ahn(Jeeyun Ahn) 대한안과학회 2023 Korean Journal of Ophthalmology Vol.37 No.1

Purpose: To investigate factors associated with refractive outcomes after phacovitrectomy for epiretinal membrane (ERM). Methods: Retrospective review of patients undergoing phacovitrectomy for ERM was done. The main outcome measure was predictive refraction error (PE), defined as observed refraction error – target refraction error, calculated by the SRK/T, Haigis, and SRK II formulae. PE was measured at postoperative 1, 3, and 6 months. Simple and multiple linear regression analysis were used to evaluate factors associated with PE. Results: A total of 53 eyes of 53 patients were included. The mean PEs at postoperative 1, 3, and 6 months were all negative, implying myopic shift in all patients regardless of the intraocular lens formula used. Haigis formula showed the least myopic shift among the three formulae (p = 0.001, Friedman test). There was no significant difference in PE depending on preoperative central macular thickness (CMT) in subgroup analysis. On stepwise multiple linear regression analysis, ERM etiology (β = 0.759, p = 0.004, SRK/T formula; β = 0.733, p = 0.008, Haigis formula; β = 0.933, p < 0.001, SRK II formula), preoperative anterior chamber depth (β = –0.662, p = 0.013, Haigis formula; β = –0.747, p = 0.003, SRK II formula), and decrease of CMT (β = –0.003, p = 0.025, SRK/T formula) were significantly associated with PE at postoperative 6 months. Conclusions: Myopic shift in PE was observed after combined phacovitrectomy for epiretinal membrane. ERM etiology, preoperative anterior chamber depth, and decrease of CMT were significantly associated with PE at postoperative 6 months. There was no difference in PE after surgery between the two groups defined by CMT (≥500 and <500 μm).