Alterations in DNA methylation patterns in regenerated Chinese cabbage (Brassica rapa ssp. pekinensis) plants derived from tissue culture

Hyun‑Min Lee,Jee‑Soo Park1,Yun‑Hee Shin,박영두 한국원예학회 2021 Horticulture, Environment, and Biotechnology Vol.62 No.4

Plant tissue culture is an essential tool in biotechnology. However, tissue-cultured plants often exhibit variations that are either genetic or epigenetic in origin, termed somaclonal variations. Among these variations, DNA methylation is an important heritable epigenetic modification that plays a role in a wide variety of biological processes, including gene expression. In this study, we performed bisulfite sequencing of regenerated Chinese cabbage (Brassica rapa ssp. pekinensis) lines to identify DNA alterations induced by tissue culture. Sequencing data from each regenerated line were compared with reference genome sequences, and common differentially methylated regions (DMRs) were detected in the regenerants. To determine changes in expression levels of DMR-containing genes, we performed quantitative reverse transcription-polymerase chain reaction (qRT-PCR) of the target genes and PCR amplification with bisulfite-converted DNA. We identified DMRs between a non-regenerant line and regenerant lines and selected 10 DMR-associated genes that presented annotation information in Arabidopsis or Brassica rapa. Their expression levels were verified by qRT-PCR to determine the relation between methylation state and gene expression. We observed that genes positioned in DMRs significantly correlated with differential gene expression. We also observed similar methylation patterns in the selected DMRs by PCR-based methylation analysis. The results of this study are a valuable resource for the epigenetic analysis of regenerated lines, especially for Chinese cabbage.

Bortezomib과 Dexamthasone으로 치료한 골수외 형질세포종 4예

백종현,이은영,장리라,손창배,신은경,서정아,이지숙,이호섭,이상민,신성훈,김양수 고신대학교의과대학 2007 고신대학교 의과대학 학술지 Vol.22 No.2

Despite the use of aggressive local and systemic treatment including autologous stem cell transplantation in multiple myeloma, extramedullary recurrences are common and the prognosis of these patients is poor. Many novel drugs such as thalidomide, lenalidomide and bortezomib improve the response of treatment of multiple myeloma, but some reports failed to describe thalidomide has effect in extramedullary plasmacytoma. Recent data report on the successful treatment plasmacytomas with bortezomib in patients with advanced multiple myeloma. We treated 4 relapsed or refractory extramedullary plasmacytomas with bortezomib at our institution. We recognized all these extramedullary plasmacytomas decreased and showed more than partial response. This report lends support to the efficacy of bortezomib in the treatment of plasmacytoma and describes the safe use of bortezomib. Responses may, however, be of short duration. Therefore, despite our limited experience, we propose that bortezomib may be considered a therapeutic option for such patients who have risk of radiation therapy

명청교체기 인평대군 시문학 연구

이현지(Lee, Hyun-jee) 한국어문학회 2016 語文學 Vol.0 No.132

Royal families were supposed to live idle lives, but the life of Prince Inpyeong was an exceptional case. Prince Inpyeong"s role has been evaluated as the absolute peace in the King Hyojong era. To solve sensitive diplomatic issues between Choson-Qing smoothly, Inpyeong took the critical role of an envoy. He took on almost all the difficult tasks in diplomacy. All his diplomatic journeys and achievements had the source in the close loving relationship with the king. The prince played a crucial role to cause the fall of Ming Dynasty and watched Qing Dynasty during the journeys. He provided information of the internal affairs of Qing Dynasty to King Hyojong. It was a significant source to make Hyojmg’s Plan to Conquer the North possible. His poetry was mainly to communicate with King Hyojong. The poems seek after a hero, enjoy the feeling of freedom elegantly, show sentiment of friendship and loyalty for his king and brother, Hyojong.

柴胡四物湯이 CCl₄로 誘發된 白鼠의 肝損傷에 미치는 影響

李在馥,白泰鉉,河智容 대한동의병리학회 1997 동의생리병리학회지 Vol.11 No.2

간은 혈액을 저장하고, 승발과 투설작용으로 인체의 기혈, 경략, 장부의 활동을 조장하는 기능과 담즙형성 및 배설작용 및 정신과 사고능력의 정상기능을 유지시키며 조혈과 해독작용을 통칭한다. 간에 영향을 줄 수 있는 요인은 많으나, 최근에는 중독에 의한 손상이 증가하고 있다. 시호사물탕은 동의보감에 '治産後發熱 及熱入血室'에 사용되며, 실험적으로 아직 연구되지 아니 하였다. 본 연구에서는 Sprague-Dawley계 흰쥐에 사염화탄소로 중독시켜 간손상을 일으킨 후 시호사물탕을 40㎎/㎏, 4㎎/㎏, 0.4㎎/㎏으로 투여하여 간손상에 미치는 영향을 알아보았다. 실험의 결과 다음의 결과를 얻을 수 있었다. 1. AST는 CCI₄+식염수투여군에 비하여 시호사물탕투여A군과 시호사물탕투여B군이 유의성있는 감소를 보였으며(P〈0.05), 시호사물탕투여C군의 경우 감소하는 경향을 보였다. 2. ALT는 CCI₄+식염수투여군에 비하여 시호사물탕투여A군과 시호사물탕투여B군이 유의성있는 감소를 보였으며 (P〈0.05), 시호사물탕투여C군의 경우 감소하는 경향을 보였다. 3. 총 Bilirubin의 경우 CCI₄+식염수투여군에 비하여 시호사물탕투여A군과 시호사물탕투여B군이 유의성있는 감소를 보였으며(P〈0.05), 시호사물탕투여C군의 경우 감소하는 경향을 보였다. 4. Albumin의 경우 CCI₄+식염수투여군에 비하여 시호사물탕투여A군, 시호사물탕투여B군, 시호사물탕투여C군 모두 유의성있는 증가를 보였다.(P〈0.05). 5. ALP의 경우 CCI₄+식염수투여군에 비하여 시호사물탕투여A군은 유의성있는 (P〈0.05)감소를 보였으며, 시호사물탕투여B군과 시호사물탕투여C군은 감소하는 경향을 보였다. 6. LDH의 경우 CCI₄+식염수투여군에 비하여 시호사물탕투여A군, 시호사물탕투여B군, 시호사물탕투여C군 모두 감소하는 경향을 보였으나, 유의성은 없었다. 7. γ-GT의 경우 CCI₄+식염수투여군에 비하여 시호사물탕투여A군, 시호사물탕투여B군, 시호사물탕투여C군 모두 감소하는 경향을 보였으나, 유의성은 없었다. 8. 간의 중량을 측정한 결과 CCI₄+식염수투여군에 비하여 시호사물탕투여A군은 유의성 있는 감소(P〈0.05)를 보였으며, 시호사물탕투여B군, 시호사물탕투여C군은 감소하는 경향을 보였다. 이상으로 보아 시호사물탕이 CCI₄로 유발된 중독성 간손상에 대하여 ALT, ALT, 총 Bilirubin, 직접 Bilirubin, Albumin, ALT에 대하여 유의성있는 감소(P〈0.05)를 보였으며, 간 손상의 회복에 대한 치료효과가 있는 것으로 사료된다. This study was carried out to investigate effects of Sihosamultang extract on the recovery of liver function in CCl₄-intoxicated rats, In this study, Sprague-Dawley Rats was divided into 5 experimental groups; Control(Placebo, 0,85% NaCl), Experimental(CCl₄-intoxicated + Placebo, 0.85% NaCI), SST-A(Treated with 40㎎ of Sihosamultang extract per ㎏, b.w after CCl₄-intoxicated), SST-B(Treated with 4㎎ of Sihosamultang extract per ㎏, b.w after CCl₄-intoxicated), SST-C(Treated with 0.4㎎ of Sihosamultang extract per ㎏, b.w after CCl₄-intoxicated). Biochemical assays for AST, ALT, Bilirubin, Albumin, ALP, LDH, γ-GT were performed. The results obtained were summarized as follows; 1. SST-A, SST-B groups showed lower serum AST level than Experimental group significantly(P〈0.05), and SST-C group showed slightly lower level than Experimental group. 2. SST-A, SST-B groups showed lower serum ALT level than Experimental group significantly(P〈0.05), and SST-C group showed slightly lower level than Experimental group. 3. Total Bilirubin level in serum were lower in SST-A, SST-B groups than in Experimental group significantly(P〈0.05), and SST-C group showed slightly lower level than Experimental group. 4. As to Albumin amount SST-A, SST-B and SST-C groups showed higher level than Experimental group significantly(P〈0.05). 5. As to tested ALP, SST-A group showed lower level than Experimental group significantly(P〈0.05), and SST-B, SST-C group showed slightly lower level than Experimental group. 6. LDH or γ-GT amount were lower in SST-A, SST-B, SST-C groups than Experimental group. 7. SST-A showed lower liver wet weight than Experimental group significantly (P 〈0.05), and SST-B, SST-C groups exhibited a tendency to lower liver wet weight than Experimental group.

복수과 진료 환자의 중복 처방에 대한 분석 및 평가

이지은,장혜경,오지영,유윤경,김현지,임숙인,연숙희,강진숙,최귀령 한국병원약사회 2003 병원약사회지 Vol.20 No.1

In an aging society, there is an increasing possibility of the duplication of the drugs given to patients because they take many kinds of drugs. Among the prescriptions given to the patients who was treated at multiple ambulatory clinics in St. Mary's hospital for one month of September, 2002, in which drugs that has identical or similar effects are prescribed, we analysed and evaluated them by patients' characteristics, kinds of medication, severity of side effects on a case by case basis. More, we assessed the potential additional costs. As a result of this study, the duplication rate turned out to be 6.69% and it was shown that the proportion of the elderly patients over 60 was high. The gastro-intestinal medications took up a large part and most of the cases showed that the danger caused by double taking of medicine was slight, but there were some examples in which the serious side effects were predicted. Expected additional costs were the average 11.349 won and it ranges from the minimum of 56 won to the maximum of 135,720 won. In conclusion, the management of drug histories of the patients who need the plural treatments is very important and the necessity of the individual and professional guidance of taking medicines for the elderly patients is emerged, too.

우측 대장에 발생한 연소성 용종 1예

이준식,박성한,홍현진,안광순,김지연,배용목,이은영,박선자 고신대학교의과대학 2007 고신대학교 의과대학 학술지 Vol.22 No.2

Juvenile polyp, also known as a retention polyp because they contain mucin cysts, is a relatively common hamartomatous lesion in chlidhood. It occurs in 1-2% of children and young adults; however it is uncommon in adulthood. Juvenile polyp is rare in the first year of life and is thus presumed to be acquired and not congenital. It is pedunculated, 2-3 cm in size, red-tan in colour, smooth and always shows fluid filled cystic spaces on cut surface. Histologically, Juvenile polyps are hamartomatous with distended, mucus-filled glands, often with cystic dilatation and edematous lamina propria containing abundant vasculature. The most common manifestation is rectal bleeding produced by auto-amputation of the polyp. Juvenile polyp has been considered not to be at increased risk of developing carcinoma. However, atypical epithelium is observed in some lesions, especially in larger polyps, which may give rise to adenoma and subsequent malignant transformation. Therefore the diagnosis of solitary juvenile polyp should not be made only with colonoscopy and biopsy, but should be totally resected endoscopically for pathologic evaluation. About 90% of the lesions are solitary and localized within 20cm from anal verge. In Korea, there are some reports of juvenile polyps located in the rectosigmoid colon. We report one case of juvenile polyp in adolescent that presented as a hematochezia & abdominal pain, which is located at right colon.

집단 따돌림 피해학생을 대상으로 한 인지행동적 집단치료 개발을 위한 예비 연구

이주현,신지용,연병길,한창환 대한소아청소년정신의학회 2000 소아청소년정신의학 Vol.11 No.2

본 연구는 집단 따돌림 피해학생을 대상으로 시행한 인지행동적 집단치료의 개발을 위한 예비 연구이다. 정상적인 중학교 1학년 학생 202명에게 도덕발달수준척도, 오퍼 자아상척도, 우울 및 상태ㆍ특성불안척도의 분포와 상관관계를 조사하였다. 자아상과 우울, 불안은 서로 강한 상관관계를 보였으나, 도덕발달과는 상관관계를 보이지 않았다. 이는 도덕발달이 정서에 크게 영향받지 않고 인지 발달적 과정에 의존하는 것을 시사한다. 그리고 교사의 관찰에 의해 집단 따돌림 피해를 받으면서 학교 적용에 어려움을 보이는 중학교 1학년 학생 5명의 도덕발달수준척도, 오퍼 자아상척도, 우울 및 상태ㆍ특성불안척도는 전체 집단과 유의한 차이를 보이지 않았다. 이 피해학생들을 대상으로 12회에 걸쳐 인지적 집단 정신치료를 시행하고, 치료 전후에 상기 4가지 척도의 변화를 평가하여 효용성을 검정하였으나 통계학적으로 유의한 변화는 없었다. 그러나 참가 학생들이 치료 기간 중에 기재한 일기에서 확인되는 심리적 마음자세(psychological mindedness)의 향상과 담임 교사의 긍정적인 평가보고는 의미있는 것으로 생각되었다. 중심 단어 : 집단 따돌림ㆍ인지행동적 집단치료ㆍ청소년ㆍ자아상ㆍ도덕발달ㆍ우울ㆍ불안 Bullying is the intentional, unprovoked abuse of power by one or more children to inflict pain on or cause distress to another child on repented occasions. Bullying can be considered to be a form of child abuse : peer abuse. Victims suffer adverse effects in the short and long term. If bullying is regarded as a form of abuse then professionals have a duty to detect it, take it seriously, and ensure that it is deal with to reduce the child's suffering and minimize the potential long term effects. This study is aimed preliminarily the development of cognitive behavioral group therapy for school bullied. 202 normal middle school freshman responded to self report scales-Offer's Self-Image Questionnaire-Revised, Children's Depression Inventory, Spielberger State/Trait Anxiety scale, and Defining Issues Test. The data were analyzed with Pearson's correlation to test the relationship among the above 4 variables. Self Image, depression, and anxiety were correlated strongly each other. But moral development was not correlated with self image, depression, and anxiety. Five school bullied students were referred by their teacher for school maladjustment. The author tried 12 sessions of Cognitive-Behavioral group therapy and assessed their behaviors pre- and post intervention by above 4 scales. There were no significant different intervention effects statistically. But It is meaningful that improvement of psychological mindedness in diary written by subject in treatment sessions KEY WORDS : School bullyingㆍCognitive-behavioral group therapyㆍAdolescentㆍSelf imageㆍMoral developmentㆍDepressionㆍAnxiety

재발성 복부 통증을 호소한 환자에서 복벽에 발생한 자궁내막증 1예

홍현진,박성한,이준식,배용목,김지연,안광순,박선자 고신대학교의과대학 2008 고신대학교 의과대학 학술지 Vol.23 No.1

Endometriosis is the abnormal growth of endometrial tissue outside the uterine cavity. Endometriosis in the abdominal scar following cesarean section is very rare condition among the extrapelvic endometriosis. The frequency of abdominal wall endometriosis secondary to cesarean section is from 0.03% to 0.8% in some reports. It is difficult to diagnosed in spite of the typical symptoms. The typical symptom is aching swelling in the area of the surgical scar, which is influenced by the phases of menstruation. The definitive diagnosis is established by pathologic analysis. Surgical excision remains the treatment of choice. The majority of patients presented from 1 to 2 years after the precipitating operation. We experienced an unusual case of abdominal wall endometriosis presented over 10 years after cesarean section, who was admitted to our hospital complaining of recurrent left lower abdominal pain. So, we report this case with a brief review of the concerned literatures.

우리나라 기업의 조세혜택과 내재적 조세에 관한 실증적 연구

이양현,김지범,윤지환 중앙대학교 산업경영연구소 2000 산업경영연구 Vol.9 No.1

본 연구는 Scholes & Wolfson(1992) 의 「Taxes and Business Sstrategy」에서 효율적으로 세무계획을 하려면 투자와 자금조달결정에 있어서 외형적 조세(Explicit Tax)뿐만 아니라 내재적 조세(Implicit Tax)와 같은 간접적인 세금의 효과도 고려해야 한다는데 착안하여 우리 나라 기업소득에 대한 내재적 조세를 이론적으로 분석하고, 실증적으로 검증하였다. 그리고 내재적 조세의 발생에 가장 큰 영향을 미치는 원인변수인 조세혜택에 기업특성변수가 미치는 영향을 분석하였다. 분석결과 조세혜택기업(租稅惠澤企業)과 조세비혜택기업(租稅非惠澤企業) 사이에 부채비율, 수출비율, 자본집약도입비율, 이익증가율 등은 유의적인 차이가 있었지만, 기업규모, 연구개발비율은 유의적인 차이를 보이지 않았다. 또한 기업에 대한 조세혜택의 증가가 세전자기자본수익률의 감소를 가져올 것이라는 내재적 조세가설(內在的 租稅假說)을 실증분석한 결과 표본기간에 걸쳐 표본전체기업을 대상으로 한 분석에서는 독립변수인 세전자기자본조세혜택비율(稅前自己資本租稅惠澤比率)과 종속변수인 세전자기자본수익율(稅前自己資本收益率) 간에는 유의적인 음(-)의 상관관계가 나타나 내재적 조세가 존재하는 것으로 나타났다. Previous horizontal equity studies have measured the variation in explicit taxes only, when both implicit and explicit taxes are considered, apparent horizontal in equity disappear and all that remains is variation due to market imperfection. Researchers have long known of suspected that omitting implicit taxes is a problem in the examining of horizontal equity on the total tax burden. Scholes and Wolfson's(1992) definition of implicit taxes and the advent of experimental markets research provide opportunities to demonstrate the effects of implicit taxes on the measurement of horizontal equity. The concept of implicit tax is based on perfectly competitive market at equilibrium. So the after-tax returns of all investments are equal after adjusting for risk. The empirical test was complied to these two sub samples. tax-advantaged firms and tax-disadvantaged firms. The result of study provides an empirical evidence that there exists a significant inverse relation between pre-tax return and tax subsidy among only tax-disadvantaged firms listed on the korean stock market. Results of previous empirical studies are consistent with the predictions of implicit tax by hypothesis and are statistically significant inverse relation between tax subsidy(PTTSE) and pre-tax return(PTROE), but the empirical relation is weaker than predicted for a pertectly competitive and frictionless market, which is due to presence of market frictions or systematic measurement error. There finding should allow policy makers to enact more effective legislation and enable researchers to construct experiments that have greater power and more internal validity.

HIV 감염 환자에서 발생한 편평 콘딜롬 1예

이해웅,오상현,장성은,최지호,문기찬,고재경 대한피부과학회 2006 大韓皮膚科學會誌 Vol.44 No.4