사회적 음주자에서 날트렉손 투여가 급성 음주 반응에 미치는 영향

김종현,김성곤,신성현,박제민,김명정 大韓神經精神醫學會 2004 신경정신의학 Vol.43 No.6

Objectives : We investigated the effects of naltrexone on acute alcohol response, stimulant and sedative, in healthy social drinkers using two doses of alcohol intake. Methods : Twenty four healthy male medical students were voluntarily participated. The experimental method was Cross-over design, Subjects received 25 mg/day or 50 mg/day of naltrexone on the experimental days. Biphasic Alcohol Effects Scale (BAES), alcohol craving, and blood alcohol concentration (BAC) were measured before drinking and at 15, 30, 45, 60, 90,and 120 min after drinking. Results : 1) Group of 0.6 mg/kg of alcohol intake. When the scores of stimulative subscale of BAES were compared bet-ween the naltrexone and control group, the scores were significantly lower in the naltrexone group at 15 and 90 min after drinking. Alcohol induced sedative effect was significantly higher in the naltrexone group at 90 min after drinking. The alcohol induced alcohol craving at 45 and 60 min after drinking was significantly lower in the naltrexone group as compared to the control. 2) Group of 0.3 ing/kg of alcohol intake. The alcohol induced stimulative effect evident in the control group seen in the time span of 15 to 45 min after drinking was not seen in the naltrexone group. The increase of alcohol induced alcohol craving noticed at 30 min after drinking in the control group was not seen in the naltrexone group. BAC at 15 min after drinking was lower in the naltrexone group compared to the control. Conclusion : Naltrexone is suggested to attenuate stimulative effect, to intensify sedative effect, and to block alcohol induced alcohol craving. These triple actions might be utilized for treatment and prevention of relapse of alcohol dependence.

초기 자궁내막암 환자에서의 복강경유도하 질식 자궁절제술과 복식 자궁절제술에 대한 비교 연구

김민규 ( Min Kyu Kim ),최동석 ( Dong Seok Choi ),김우영 ( Woo Young Kim ),최철훈 ( Chel Hun Choi ),김태중 ( Tae Joong Kim ),이정원 ( Jeong Won Lee ),김병기 ( Byoung Gie Kim ),이제호 ( Je Ho Lee ),배덕수 ( Duk Soo Bae ) 대한산부인과학회 2006 Obstetrics & Gynecology Science Vol.49 No.12

목적: 초기 자궁내막암의 수술적치료 시 복강경유도하 질식 자궁절제술 및 골반림프절절제술군과 복식수술군의 수술결과 및 재발율에 대해 비교하고자하였다. 연구 방법: 2003년 3월부터 2005년 5월까지 초기 자궁내막암 환자를 인구학적 특성이 같은 63명을 선택하여 복강경유도하 질식 자궁절제술 및 골반림프절절제술군 26명, 복식수술군 37명으로 나누어 수술 전후 혈색소 차이, 평균수술시간, 취득한 골반림프절갯수, 평균재원일수, 추가치료율, 재발률을 비교하였다. 결과: 복강경유도하 질식 자궁절제술 및 골반림프절절제술을 시행한 군과 복식수술을 시행한 군과의 비교에서 수술 전후 혈색소 차이, 평균수술시간, 취득한 골반림프절 갯수, 평균재원일수, 재발률에 통계적으로 유의한 차이가 없었다. 결론: 초기 자궁내막암의 수술 시 복강경유도하 질식자궁절제술 및 골반림프절절제술 방법이 복식방법에 비해 수술결과 및 재발률에 통계적으로 유의한 차이가 없으며 그 대체 수술법으로 많은 활용이 기대되었다. Objective: To compare laparoscopic surgery with conventional abdominal surgery in patients with early stage endometrial cancer. Methods: A retrospective review of 63 patients with early stage endometrial cancer managed between March 2003 and May 2005. Two groups were defined whether they had been treated by laparoscopy (case group: n=26) or by laparotomy (control group: n=37). We compared age, body mass index (BMI), hemoglobin change, operation time, number of pelvic lymph nodes, hospital stay, case with adjuvant treatment and recurrence between two groups. Results: There was no statistical difference in characteristics (age, BMI, nulliparity, previous abdominal surgery, FIGO stage, histologic grade). between case and control group. In addition, there was no statistical difference in operation data and outcomes between two groups. Hemoglobin changes were 1.1 g/dL (case group) vs 1.7 g/dL (control group) (p=0.072). Operation time was 131 min vs. 115 min. The numbers of lymph nodes obtained were 8.7 vs 7.7 (right) 9.2 vs. 7.6 (left). Hospital stays were 8.4 vs. 9.2 days. Adjuvant treatment cases were 7 vs. 15. Recurrent case was one in each group. Two patients initially evaluated by laparoscopy were converted to laparotomy due to bleeding and adhesion. Conclusion: Laparoscopic surgery for treatment of early endometrial cancer is a safe and effective alternative to laparotomy . However, long-term survival and risk of recurrence have yet to be determined.

都市 下水슬러지의 堆肥化에 關한 硏究

宋濟天,柳明辰,金東玟 동아대학교 환경문제연구소 1986 硏究報告 Vol.9 No.3

Composting may be defined as a biochemical process in which microorganisms break down and convert solid organic matters into stable, humus like end products. The composting of municipal wastewater sludge has become an accepted technology as a means of sludge stabilization and resource recovery in recent years. Investigated in this study were the compostability of municipal sludge without the use of bulking agents, e.g., wood chips, tree barks, sow-dusts and rice-hulls ; relevant, influencing factors such as moisture contents, pH values, aeration rates, and volatile solid contents ; and process reaction rates. The sample sludges, obtained from Seoul Cheongye Sewage Treatment plant, were those digested and dewatered by mechanical means with the aid of lime and ferric chloride. The pH values, moisture contents, voltile solid contents and C/N ratios of the sample sludge ranged 10 to 12, 60 to 70%, 55 to 55% and 8 to 12, respectively. In this study, three, 35-liter reactors were used; the reactors were operated on batch basis for the duration of 8 to 12days. In order to maintain aerobic conditions and expect air drying effects, air was supplied to each reactor in the rates of 50-300 ℓ/㎥, main in the first half of experiments and fixed to 120ℓ/㎥. min in the second half. The mixture was mixed completely once a day by mechanical agitator. The results of this study follow; 1. The municipal sludge can be composted without the use of bulking agents. 2. The composting of the sole sludge without any additives can be carried out in reduced reaction periods 1 to 2 weeks. 3. The compost sludge can be used as both, the soil conditioner and low-nutrient fertilizer. It contained 35 to 40% moisture, 35 to 45% valatile solids, 1.9 to 2.2% nitrogen, 2.1 to 2.5% phosphorus and 0.18 to 0.22% potassium, and was 7.5 to 8.0 in pH values and 8 to 10 in C/N ratios. 4. The optimum pH values, aeration rates, and moisture contents were 7.5 to 8, approximately 120ℓ/㎥.min and 55 to 60%, respectively.

High-performance Liquid Chromatographic Analysis for Quantitation of Marker Compounds of Artemisia capillaris Thunb

( Kyung Min Park ),( Ying Li ),( Bora Kim ),( Haiyan Zhang ),( Kyong Hwangbo ),( Dong Gen Piao ),( Mei Juan Chi ),( Mi Hee Woo ),( Jae Sue Choi ),( Je Hyun Lee ),( Dong Cheul Moon ),( Hyeun Wook Chang 영남대학교 약품개발연구소 2013 영남대학교 약품개발연구소 연구업적집 Vol.23 No.0

Two stable high-performance liquid chromatography (HPLC) methods were developed that could quantitatively analyze 10 major marker compounds of Artemisia capillaris Thunb and could also distinguish among `Injinho` and `Myeon-injin` and `Haninjin`--A. capillaris collected in autumn, A. capillaris collected in spring and A. iwayomogi, which can be misused as `Injinho` in Korean herbal drug markets. The first HPLC method was a reversed-phase chromatography using a C18 column with an isocratic solvent system of phosphoric acid (0.05%) and acetonitrile at the flow rate of 1.0 mL/min, ultraviolet (UV) detection wavelength at 254 nm and column temperature at 40°C. Calibration andquantitation were made by using acetaminophen as an internal standard (I.S-A) and chlorogenic acid (1) was determined within 20 min. The second HPLC method was a reversed-phase chromatography using a C18 column with a gradient solvent system of phosphate buffer (0.015 M, pH 6) and acetonitrile at the flow rate of 1.0 mL/min, UV detection wavelength at 254 nm and column temperature at 40°C. Calibration and quantitation were made by using ethylparaben as an internal standard (I.S-B) and 3,5-di-O-caffeoylquinic acid (2), 3,4-di-O-caffeoylquinic acid (3), 4,5-di-O-caffeoylquinic acid (4), hyperoside (5), isoquercitrin (6), isorhamnetin 3-O-robinobioside (7), isorhamnetin-3-O-galactoside (8), isorhamnetin-3-O-glucoside (9) and scoparone (10) were determined within 60 min. Pattern recognitionanalysis of data from the 60 samples classified them clearly into three groups. These assay methods could be applied for QA/QC of A. capillaris and Artemisia iwayomogi.

흰쥐의 중추성 Serotonin 고갈이 강제수영 중 부동자세 시간, 불안 및 수동적 회피 학습에 미치는 효과

박제민,김종백,강철중 大韓神經精神醫學會 1996 신경정신의학 Vol.35 No.3

흰쥐에서 p-chlorphenylalanine(PCPA)로 인한 강제수영 중 부동자세 시간의 단축이 불안이나 수동적 회피 학습 능력의 변화와 관련된 것인가를 검증하고자 하였다. 흰쥐에게 PCPA 300mg/kg 혹은 생리식염수를 하루 한 번 연속 3회 복강내 주사하였다. 마지막 주사 24시간 후에 공간에서의 활동성(ACT0), 높은 십자 통로 실험에서 개방 통로에 머무는 시간(ANX0), 왕복 회피상자에서 통과 지연 시간(STL0) 등을 측정하고 0.5mA 전기자극을 3초간 주어 회피 반응을 학습시켰다. 그리고 15분간 강제수영시켰다. 다음 날 같은 방법으로 활동성(ACT1), 불안(ANX1), 수동적 회피 반응 학습 정도(STL1), 5분간의 강제수영 중 총 부동 자세 시간 등을 측정하였다. 마지막 약물 혹은 대조액 투여 7일 후 수동적 회피 학습 유지 정도(STL7)를 다시 측정하였다. 결과는 아래와 같다. 1) 전체 대상군에서 ACT1은 ACT0보다 감소하였다. PCPA는 개방공간에서의 활동성에 영향을 주지 않았다. 2) PCPA 군은 높은 십자 통로 실험에서 ANX0와 ANX1 둘 다 대조군 보다 유의하게 길었다. 3) STL1은 PCPA군과 대조군 모두 STL0 보다 유의하게 지연되었다. PCPA는 STL0 이나 STL1에 영향을 주지 않았다. 그러나 대조군은 수동적 회피 학습 7일 후에도 통과 지연 시간이 유의하게 연장되어 있는데 반하여 PCPA군은 STL7이 STL0과 유의한 차이가 없었다. 4) 강제수영 중 부동자세 시간은 PCPA군이 대조군보다 유의하게 짧았다. 이상의 결과에서 PCPA 투여로 인한 강제수영 중 부동자세 시간의 단축은 PCPA가 비특이적 신체 활동을 증가시키거나 수동적 회피 반응 습득을 방해함으로써 일어나는 현상이 아니며, 진정 작용없이 탐색 행동을 증가시키거나, 스트레스 하에서의 행동 억제 현상을 차단함으로써 강제수영 중 부동자세 시간을 단축시킨다고 추론한다. 아울러 PCPA가 기억 고정 과정을 방해할 가능성이 있음을 제안한다. Purpose : The test if p-chlorophenylalanine(PCPA) induced decrease of immobility time during forced swimming test in rats is an epiphenomenon related with PCPA-induced changes in anxiety level or learning ability. Methods : 22 male rats(Sprague Dawley, weighting 180-280gm) were randomly assigned to PCPA or control group and treated with 300mg/kg of PCPA or saline i.p. at -72, -48, and -24 hrs. Number of segment(ACT0) entered with 4 limbs on the open field(L100×W100×H40cm, 25 segments), time(ANX0, sec) spent on the open arm(L110×W10cm, 50cm from the ground) in the elevated plus maze, and step-through latency(STL0, cut-off time : 300sec) in the shuttle box(L76.2×W34.3×H50.8cm, 7.5w electric bulb in the light chamber, 0.5mA for 3 sec in the dark chamber) were measured consequently at 24hrs after the last injection of PCPA or saline. After 20min of rest, animals were forced to swim for 15 min(cylinder : φ18×H40cm ; water : H15cm, 25℃). On the next day, the second trial of open field test(ACT1), elevated plus maze test(ANX1), passive avoidance learning test(STL1), and measurement of total immobility time(IT, sec) during the 5min of forced swim were done in the same manner as the first trial except that electrical shock was not given to the animals in the passive avoidance learning test and duration of forced swimming was shortened from 15min to 5min. To evaluate longterm effect of PCPA on maintenance of memory, measurement of step-through latency was repeated 7 days after the first trial(STL7). Result: 1) ACT1 was significantly decreased in the pooled sample, but the effect of PCPA on the general motor activity was not significant. 2) PCPA significantly prolonged the time spent on the open arms in the elevated plus maze test. 3) Comparing to STL0, STL1 was significantly prolonged in both groups. PCPA did not affect STL0 or STL1. STL7 was significantly prolonged in the control group, but not in the PCPA group. 4) PCPA shortened IT significantly. Conclusion : PCPA-induced decrease of immobility time during the forced swimming test in rats seems to be related with disinhibition of stress-related behavioral suppression. PCPA does not affect acquisition and short-term maintenance of passive avoidance learning, but necessity of further studies about its effect on long-term maintenance on memory is raised.

오죽잎차와 오죽죽순차의 성분 분석 및 항산화 효과

김상민 ( Sang Min Kim ),전제승 ( Je Seung Jeon ),강석우 ( Suk Woo Kang ),김우리 ( Woo Ri Kim ),이기덕 ( Ki Deok Lee ),엄병헌 ( Byung Hun Um ) 한국응용생명화학회(구 한국농화학회) 2012 Journal of Applied Biological Chemistry (J. Appl. Vol.55 No.2

Nutritional components of Ojuk leaf tea and Ojuk shoot tea prepared from the leaves and shoots of black bamboo (Phyllostachys nigra Munro) by tea manufacturing process were evaluated. In addition, the extraction yield of water soluble components from these teas in the general tea brewing condition (water extraction in 80oC for 10 min.) and the contents of polyphenol and flavonoid were compared with not only the dried raw materials, but also green tea and mate tea. Finally, offline and online scavenging activities against 2,2`-azino-bis(3-ethylbenzothiazoline- 6-sulphonic acid) (ABTS) and di(phenyl)-(2,4,6 trinitrophenyl)iminoazanium free radicals were investigated to evaluate the antioxidant activity and explore the components showing ABTS free radical scavenging activity from tea infusion. These results demonstrated that these teas from black bamboo contain various nutritional components and can be used as traditional tea beneficial to human health.

Geometric effect of the hydrogel grid structure on in vitro formation of homogeneous MIN6 cell clusters.

Bae, Chae Yun,Min, Mun-kyeong,Kim, Hail,Park, Je-Kyun Royal Society of Chemistry 2014 Lab on a chip Vol.14 No.13

<P>A microstructure-based hydrogel was employed to study the relationship between spatial specificity and cellular behavior, including cell fate, proliferation, morphology, and insulin secretion in pancreatic β-cells. To effectively form homogeneous cell clusters in vitro, we made cell-containing hydrogel membrane constructs with an adapted grid structure based on a hexagonal micropattern. Homogeneous cell clusters (average diameter: 83.6 14.2 μm) of pancreatic insulinoma (MIN6) cells were spontaneously generated in the floating hydrogel membrane constructs, including a hexagonal grid structure (size of cavity: 100 μm, interval between cavities: 30 μm). Interestingly, 3D clustering of MIN6 cells mimicking the structure of pancreatic islets was coalesced into a merged aggregate attaching to each hexagonal cavity of the hydrogel grid structure. The fate and insulin secretion of homogeneous cell clusters in the hydrogel grid structure were also assessed. The results of these designable hydrogel-cell membrane constructs suggest that facultative in vitro β-cell proliferation and maintenance can be applied to biofunctional assessments.</P>

주정중독 환자에서 Fluoxetine이 혈중 알콜 농도와 음주효과에 미치는 영향

김명정,박제민,정봉주 大韓神經精神醫學會 1994 신경정신의학 Vol.33 No.1

The effect of chronic administration of fluoxetine on blood alcohol concentration and on a number of alcohol induced physical and psychological effects was studied. The subjects were 14 hospitalized alcoholics without significant depression. After 21 days of 60mg daily fluoxetine and placebo administration. 2.5ml/kg body weight of 40% alcohol was administrated in a single dose and blood alcohol concentrations were measured 10, 20, 40, 60, 120, and 240min, after the alcohol intake. Mania Rating Scale score(MRS), pulse rate, respiratory rate and subjective physical condition were measured before and 30min, after alcohol intake. The results were as follows: 1) Blood alcohol concentrations were significantly higher after fluoxetine administration than placebo administration at 40min, and 60min, after alcohol intake. 2) Fluoxetine suppressed the increase of pluse rate induced by alcohol intake. However, no significant difference was noted in the alcohol induced changes in the scores of MRS, respiratory rate, and subjective physical condition between the drug and placebo administrations. 3) After fluoxetine administration, a significant negative correlation was shown between blood alcohol concentration, 10min after alcohol intake and MRS. In contrast, however, a significant positive correlation was seen between the two variable after placebo administration. From these results, it is suggested that fluoxetine increases blood alcohol concentration only during higher alcohol concentration and that the reduction of alcohol intake after chronic administration of fluoxetine may be due, at least in part, to an attenuation of positie reinforcement of alcohol effect, rather than to an enhancement of the aversion reaction to alcohol.

Protective Effects of N-acetylcystenine and Selenium against Doxorubicin Toxicity in Rats

Yeo-sung Yoon,Sun-don Kim,Min-hye Lee,Heung-shik S.Lee,In-se Lee,Je-kyung Sung,Eun-sung Park 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.2

Effects of N-acetylcysteine and Selenium against Doxorubicin Toxicity in RatsEun-sung Park, Sun-don Kim1, Min-hye Lee, Heung-shik S. Lee, In-se Lee, Je-kyung Sung and Yeo-sung Yoon*

대두요구르트제조를 위한 두유에서의 젖산균의 산생성과 침전에 관한 연구

강성태,정종민,김제중,정병옥,이흠숙,정석진 서울産業大學校 1999 논문집 Vol.49 No.2

전지두유에 시유를 각각 다른 비율로 혼합하여 제조하여 L. bulgaricus를 접종한 후 산생성에 미치는 효과를 조사하였다. 우유의 첨가량을 늘릴수록 24시간 후의 적정산소가 증가하여 두유에 유유를 혼합시키는 것이 L. bulgaricus에 의한 산생성에 유리함을 알 수 있었다. 전지두유를 100℃에서 30분, 60분, 90분간 가열하고 121℃에서 15분간 열처리하여 L. bulgaricus와 L. casei를 단독으로 접종하거나 1:1(v/v)로 혼합하여 접종하고 두유의 열처리가 산생성과 커드의 침전에 미치는 영향을 평가하였다. 두유의 열처리는 전반적으로 열처리되지 않은 두유보다 산생성을 촉진하였으며 산생성은 가열처리 정도에 비례하였다. 또한 모든 배양에서 121℃ 15분간의 열처리는 100℃ 90분의 열처리보다 낮은 산생성을 보여주었다. 또한 두유의 열처리는 제조된 두유요구르트의 커드의 침전을 지연시키는 효과가 있었다. Soy milk was added with different ratio of market milk. Acid production of L. bulgaricus in mixtures were investigated. Acid production by L. bulgaricus increased in proportion to concentration of milk added to soy milk. And the effects of various heat treatment of soy milk on acid production by L. bulgaricus and L. casei alone or combined were investigated. Sedimentation characteristics of soy yogurt prepared from heat treated soy milk were also evaluated. Heat treatment of soy milk stimulated acid production by all cultures. Acid production was generally proportional to the degree of heat treatment. However, Heat treatment of soy milk at 121℃ for 15min showed rather lower acid production than that at 100℃ for 90 min. Heat treatment of soy milk generally retarded sedimentation of curd in soy yogurt beverage.